BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0035.0050.p
         (337 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300312096|ref|YP_003776188.1| peptide chain release factor 2 ...   696   0.0  
gi|300313560|ref|YP_003777652.1| peptide chain release factor 1 ...   223   2e-59

>gi|300312096|ref|YP_003776188.1| peptide chain release factor 2
           [Herbaspirillum seropedicae SmR1]
          Length = 337

 Score =  696 bits (1797), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 336/337 (99%), Positives = 337/337 (100%)

Query: 1   VKSEKLEQVNAELEDPNVWNDQKRAQDLGKEKKSLEAIVHTLIKIDSELTDARDLFEMAR 60
           +KSEKLEQVNAELEDPNVWNDQKRAQDLGKEKKSLEAIVHTLIKIDSELTDARDLFEMAR
Sbjct: 1   MKSEKLEQVNAELEDPNVWNDQKRAQDLGKEKKSLEAIVHTLIKIDSELTDARDLFEMAR 60

Query: 61  EEKDEDTIIAIEGDAENLKKMVEEMEFRRMFSGPMDANNCFIDIQAGAGGTEAQDWASML 120
           EEKDEDTIIAIEGDAENLKKMVEEMEFRRMFSGPMDANNCFIDIQAGAGGTEAQDWASML
Sbjct: 61  EEKDEDTIIAIEGDAENLKKMVEEMEFRRMFSGPMDANNCFIDIQAGAGGTEAQDWASML 120

Query: 121 LRQYLRYCERKGFKAEILEQSDGEVAGIKTATIKVEGEYAYGFLRTETGVHRLVRKSPFD 180
           LRQYLRYCERKGFKAEILEQSDGEVAGIKTATIKVEGEYAYGFLRTETGVHRLVRKSPFD
Sbjct: 121 LRQYLRYCERKGFKAEILEQSDGEVAGIKTATIKVEGEYAYGFLRTETGVHRLVRKSPFD 180

Query: 181 SSNGRHTSFSSLFVYPEVDESFEIEINPADVRVDTYRASGAGGQHINKTDSAVRLTHAPS 240
           SSNGRHTSFSSLFVYPEVDESFEIEINPADVRVDTYRASGAGGQHINKTDSAVRLTHAPS
Sbjct: 181 SSNGRHTSFSSLFVYPEVDESFEIEINPADVRVDTYRASGAGGQHINKTDSAVRLTHAPS 240

Query: 241 GIVVQCQNDRSQHRNRAEAWDMLRAKLFELELRKRMSEQQKLEDSKTDVGWGHQIRSYVL 300
           GIVVQCQNDRSQHRNRAEAWDMLRAKLFELELRKRMSEQQKLEDSKTDVGWGHQIRSYVL
Sbjct: 241 GIVVQCQNDRSQHRNRAEAWDMLRAKLFELELRKRMSEQQKLEDSKTDVGWGHQIRSYVL 300

Query: 301 DQSRIKDLRTNFETGNTKAVLDGDIDDFIAASLKQGV 337
           DQSRIKDLRTNFETGNTKAVLDGDIDDFIAASLKQGV
Sbjct: 301 DQSRIKDLRTNFETGNTKAVLDGDIDDFIAASLKQGV 337


>gi|300313560|ref|YP_003777652.1| peptide chain release factor 1
           [Herbaspirillum seropedicae SmR1]
          Length = 359

 Score =  223 bits (567), Expect = 2e-59,   Method: Compositional matrix adjust.
 Identities = 133/342 (38%), Positives = 200/342 (58%), Gaps = 24/342 (7%)

Query: 3   SEKLEQVNAELEDPNVWNDQKRAQDLGKEKKSLEAIVHTL---------IKIDSELTDAR 53
           +E+LE++N+ L   +        + + +E   L  +V            I+   EL    
Sbjct: 13  AERLEELNSLLAQEDATASMDNFRKMTREHAELGPLVALYHEYVQAAEDIRTAQELLADP 72

Query: 54  DLFEMAREEKDEDTIIAIEGDAENLKKMVEEMEFRRMFSGPMDANNCFIDIQAGAGGTEA 113
           D+   A+EE D      +EG   +L+KM+       +   P D  N F++I+AG GG E+
Sbjct: 73  DMKAFAQEEID-GAKARMEGLELDLQKML-------LPKDPNDERNIFLEIRAGTGGDES 124

Query: 114 QDWASMLLRQYLRYCERKGFKAEILEQSDGEVAGIKTATIKVEGEYAYGFLRTETGVHRL 173
             +A  LLR Y RY ER+ ++ EI+  S+ E+ G K    ++ G  AY  L+ E+G HR 
Sbjct: 125 ALFAGDLLRMYTRYAERQRWQVEIVSASESELGGYKEVIARIVGLGAYSRLKFESGGHR- 183

Query: 174 VRKSPFDSSNGR-HTSFSSLFVYPEVDESFEIEINPADVRVDTYRASGAGGQHINKTDSA 232
           V++ P   + GR HTS  ++ V PE DE  ++EINPAD+R+DTYRASGAGGQHINKTDSA
Sbjct: 184 VQRVPATETQGRIHTSACTVAVMPEADEVGDVEINPADIRIDTYRASGAGGQHINKTDSA 243

Query: 233 VRLTHAPSGIVVQCQNDRSQHRNRAEAWDMLRAKLFELELRKRMSEQQKLEDSKTDVGWG 292
           VR+TH P+GIVV+CQ+DRSQH+N+A+A  +L A++ +++LR++ SE+      K+ +G G
Sbjct: 244 VRITHLPTGIVVECQDDRSQHKNKAQAMKVLAARIKDVQLRQQQSEEAATR--KSLIGSG 301

Query: 293 ---HQIRSYVLDQSRIKDLRTNFETGNTKAVLDGDIDDFIAA 331
               +IR+Y   Q R+ D R N        ++DGD+D+   A
Sbjct: 302 DRSERIRTYNFPQGRMTDHRINLTLYKLDFIMDGDLDELTNA 343


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.315    0.132    0.372 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 998,716
Number of extensions: 40099
Number of successful extensions: 106
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 105
Number of HSP's successfully gapped: 2
Length of query: 337
Length of database: 1,619,870
Length adjustment: 86
Effective length of query: 251
Effective length of database: 1,212,660
Effective search space: 304377660
Effective search space used: 304377660
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 131 (55.1 bits)