BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0045.p (707 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312090|ref|YP_003776182.1| ATP-dependent DNA helicase [Her... 1436 0.0 gi|300313605|ref|YP_003777697.1| ATP-dependent DNA helicase rep ... 199 7e-52 gi|300309775|ref|YP_003773867.1| ATP-dependent DNA helicase [Her... 185 1e-47 >gi|300312090|ref|YP_003776182.1| ATP-dependent DNA helicase [Herbaspirillum seropedicae SmR1] Length = 707 Score = 1436 bits (3718), Expect = 0.0, Method: Compositional matrix adjust. Identities = 704/707 (99%), Positives = 705/707 (99%) Query: 1 MPPELIQDAPDTTSLPDRESDPFARLNAQQREAVEHGGKLPPSQQPPLLIVAGAGSGKTN 60 MPPELIQDAPDTTSLPDRESDPFARLNAQQREAVEHGGKLPPSQQPPLLIVAGAGSGKTN Sbjct: 1 MPPELIQDAPDTTSLPDRESDPFARLNAQQREAVEHGGKLPPSQQPPLLIVAGAGSGKTN 60 Query: 61 TLAHRVARLVLEGADPQRILLLSFSRRAAADMGRRVGQVISQVLGLAASDTPPQLHWSGT 120 TLAHRVARLVLEGADPQRILLLSFSRRAAADMGRRVGQVI+QVLGLAASDTPPQLHWSGT Sbjct: 61 TLAHRVARLVLEGADPQRILLLSFSRRAAADMGRRVGQVIAQVLGLAASDTPPQLHWSGT 120 Query: 121 FHAIGARLLREYAKVIGLEEDFTIQDRGDSEDLMGLLRHDMGLSATEKRFPLKATCLAIY 180 FHAIGARLLREYAKVIGLEEDFTIQDRGDSEDLMGLLRHDMGLSATEKRFPLKATCLAIY Sbjct: 121 FHAIGARLLREYAKVIGLEEDFTIQDRGDSEDLMGLLRHDMGLSATEKRFPLKATCLAIY 180 Query: 181 SRAVNTRQALGEVLQQHFPWCCEWERPLGELFGRYVDAKEEQHVLDYDDLLLFWADMLGD 240 SRAVNTRQ LGEVLQQHFPWCCEWERPLGELFGRYVDAKEEQHVLDYDDLLLFWADMLGD Sbjct: 181 SRAVNTRQDLGEVLQQHFPWCCEWERPLGELFGRYVDAKEEQHVLDYDDLLLFWADMLGD 240 Query: 241 AQIAQSVGQRFDHVLVDEYQDTNRLQAEILQRMKPDGAGVTAVGDDAQAIYSFRGATVRN 300 AQIAQSVGQRFDHVLVDEYQDTNRLQAEILQRMKPDGAGVTAVGDDAQAIYSFRGATVRN Sbjct: 241 AQIAQSVGQRFDHVLVDEYQDTNRLQAEILQRMKPDGAGVTAVGDDAQAIYSFRGATVRN 300 Query: 301 ILDFPGLFTQPVRLLTLEQNYRSTQPILHASNAVIADAPERHVKHLWSDKPSSQKPQLVV 360 ILDFPGLFTQPVRLLTLEQNYRSTQPILHASNAVIADAPERHVKHLWSDKPSSQKPQLVV Sbjct: 301 ILDFPGLFTQPVRLLTLEQNYRSTQPILHASNAVIADAPERHVKHLWSDKPSSQKPQLVV 360 Query: 361 IPDEAEQARWVANRILAHREEGLTLKSQAVLFRTATHSAALELELARRNIPFVKFGGLKF 420 IPDEAEQARWVANRILAHREEGLTLKSQAVLFRTATHSAALELELARRNIPFVKFGGLKF Sbjct: 361 IPDEAEQARWVANRILAHREEGLTLKSQAVLFRTATHSAALELELARRNIPFVKFGGLKF 420 Query: 421 LESGHIKDLLALLRLAHNPSGRMAGLRALQLIPGVGPATARKVLDLMAVAADPADALLNF 480 LESGHIKDLLALLRLAHNPSGRMAGLRALQLIPGVGPATARKVLDLMA AADPADALLNF Sbjct: 421 LESGHIKDLLALLRLAHNPSGRMAGLRALQLIPGVGPATARKVLDLMAAAADPADALLNF 480 Query: 481 GAPANIALQWQAFADTYALMRRASASWPTDILAARDWYLPQLQERYDDAQARAADIEQLA 540 GAPANIALQWQAFADTYALMRRASASWPTDILAARDWYLPQLQERYDDAQARAADIEQLA Sbjct: 481 GAPANIALQWQAFADTYALMRRASASWPTDILAARDWYLPQLQERYDDAQARAADIEQLA 540 Query: 541 VLANGYASREKFLTELTLDPPEASSDFSGPPLRDEDYLTLSTIHSAKGQEWRSVHILNVV 600 VLANGYASREKFLTELTLDPPEASSDFSGPPLRDEDYLTLSTIHSAKGQEWRSVHILNVV Sbjct: 541 VLANGYASREKFLTELTLDPPEASSDFSGPPLRDEDYLTLSTIHSAKGQEWRSVHILNVV 600 Query: 601 DGCIPSDLATGSEAEIAEERRLLYVAMTRARQYLHLVVPQRFYITQQTPQGDRHVNGIRS 660 DGCIPSDLATGSEAEIAEERRLLYVAMTRARQYLHLVVPQRFYITQQTPQGDRHVNGIRS Sbjct: 601 DGCIPSDLATGSEAEIAEERRLLYVAMTRARQYLHLVVPQRFYITQQTPQGDRHVNGIRS 660 Query: 661 RFITPRMLPHFEDSLWFSAETNGARTPMPESVRMLVRQRARQSWNKS 707 RFITPRMLPHFEDSLWFSAETNGARTPMPESVRMLVRQRARQSWNKS Sbjct: 661 RFITPRMLPHFEDSLWFSAETNGARTPMPESVRMLVRQRARQSWNKS 707 >gi|300313605|ref|YP_003777697.1| ATP-dependent DNA helicase rep [Herbaspirillum seropedicae SmR1] Length = 690 Score = 199 bits (506), Expect = 7e-52, Method: Compositional matrix adjust. Identities = 205/675 (30%), Positives = 327/675 (48%), Gaps = 96/675 (14%) Query: 20 SDPFARLNAQQREAVEHGGKLPPSQQPPLLIVAGAGSGKTNTLAHRVARLVLE-GADPQR 78 S P LN Q EAV + P L++AGAGSGKT + ++A L+ G + + Sbjct: 2 SAPSFGLNKPQSEAVHY-------MAGPCLVLAGAGSGKTRVITQKIAHLIENCGYESRN 54 Query: 79 ILLLSFSRRAAADMGRRVGQVISQVLGLAASDTPPQLHWS-GTFHAIGARLLREYAKVIG 137 I L+F+ +AA +M R+ +++ D H + TFH++G ++LR+ ++ +G Sbjct: 55 IAALTFTNKAALEMQERIAKLLK--------DPKQAKHLTVSTFHSLGVKILRQESQHLG 106 Query: 138 LEEDFTIQDRGDSEDLMGLLRHDMGLSATEKRFPLKATCLAIYSRAVNTRQALGEVLQQH 197 L++ F+I D S+D L++ L+ T KA + + + L + Sbjct: 107 LKDRFSIMD---SDDCFSLVQE---LAVTTD----KAIIRGVQNSISLWKNGLVDPDLAE 156 Query: 198 FPWCCEWERPLGELFGRYVDAKEEQHVLDYDDLLLFWADML-GDAQIAQSVGQRFDHVLV 256 E E ++ YV + +D+DDL+ ++ + ++ ++ ++L+ Sbjct: 157 KNARTEDEAQAARIYRSYVATLKAYQAVDFDDLIRLPVELFRSNEEVRDRWQRKLRYLLI 216 Query: 257 DEYQDTNRLQAEILQRMKPDGAG----VTAVGDDAQAIYSFRGATVRNI----LDFPGLF 308 DEYQDTN Q E++ ++ G G TAVGDD QAIY++RGAT+ N+ +DFP L Sbjct: 217 DEYQDTNTCQYELV-KLLVTGIGKKPMFTAVGDDDQAIYAWRGATIENLKLLGVDFPNL- 274 Query: 309 TQPVRLLTLEQNYRSTQPILHASNAVIADAPERHVKHLWSDKPSSQKPQLVVIPDEAEQA 368 L+ LEQNYRS+ IL A+NAVI++ P+ K LWS+ ++ + DE +A Sbjct: 275 ----HLIKLEQNYRSSTRILQAANAVISNNPKLFDKTLWSEHGLGDPITVMAMDDEEAEA 330 Query: 369 RWVANRILAHR-EEGLTLKSQAVLFRTATHSAALELELARRNIPFVKFGGLKFLESGHIK 427 +A + AHR E A+L+R + LE L R IP+V GG + + IK Sbjct: 331 DQIAITLSAHRFERRAKFADYAILYRGNHQARILEKSLRRERIPYVMSGGQSYFDRAEIK 390 Query: 428 DLLALLRLAHNPSGRMAGLRALQLIP--GVGPATARKVLDLMAVAADPA--DALLNFGAP 483 D++A LRL N A +RA+ P GVG AT +VL +A + +A+ G Sbjct: 391 DIIAYLRLIANQDDDPAFIRAVT-TPRRGVGQATL-EVLGTLAGQWQCSLFEAVYKGGLE 448 Query: 484 ANIA----LQWQAFAD-TYALMRRASASWPT-----------DILAARDW--YLPQLQER 525 + L + F D AL RA+ P+ D++ A ++ YL Sbjct: 449 DKLTDRQLLPLRKFCDFINALESRATRPGPSGSGENAGKVLDDMMEAINYEFYL------ 502 Query: 526 YDDAQARAAD------------IEQLAVLANGYASREKFLTELTLDPPEASSDFSGPPLR 573 YD + RAA +++ A EK L E+T + + S R Sbjct: 503 YDSFEERAAQARWQNVVDFINWLKERACGGRDGEGEEKNLLEIT----QMVALMSMLEGR 558 Query: 574 DE--DYLTLSTIHSAKGQEWRSVHILNVVDGCIP--SDLAT---GSEAEIAEERRLLYVA 626 DE D + +ST+H++KG E+ V ++ V +G +P D T + A + EERRL+YV Sbjct: 559 DEEQDAVRMSTLHASKGLEFPHVFLVGVEEGILPHKGDPDTPPEEAAARVEEERRLMYVG 618 Query: 627 MTRARQYLHLVVPQR 641 +TRA++ LHL +R Sbjct: 619 ITRAQRSLHLSWCKR 633 >gi|300309775|ref|YP_003773867.1| ATP-dependent DNA helicase [Herbaspirillum seropedicae SmR1] Length = 759 Score = 185 bits (469), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 197/706 (27%), Positives = 305/706 (43%), Gaps = 100/706 (14%) Query: 49 LIVAGAGSGKTNTLAHRVARLVLEG-ADPQRILLLSFSRRAAADMGRRVGQVISQVLGLA 107 LI+AGAGSGKT L R+A L+ G P IL ++F+ +AA +M R+ G+ Sbjct: 24 LILAGAGSGKTRVLTTRIAWLIQTGQVSPSGILAVTFTNKAAKEMTARLS-------GML 76 Query: 108 ASDTPPQLHWSGTFHAIGARLLREYAKVIGLEEDFTIQDRGDSEDLMGLLRHDMGLSATE 167 +T W GTFH + RLLR + K GL + F I D D + L M + + Sbjct: 77 PINTRGM--WIGTFHGLCNRLLRAHHKDAGLPQTFQILDTQDQLSAIKRLLKQMNVD--D 132 Query: 168 KRFPLKATCLAIYSRAVNTRQALGEVLQQHFPWCCEWERPLGELFGRYVDAKEEQHVLDY 227 +++P + I S +A + ++ R EL+ Y + + V+D+ Sbjct: 133 EKYPPRNLMYFINSAKDQGLRA------KDVDAYDDYNRKFVELYELYDQQCQREGVVDF 186 Query: 228 DDLLLFWADMLGDAQ-IAQSVGQRFDHVLVDEYQDTNRLQAEILQRMKPDGAGVTAVGDD 286 +LLL ++L Q + + RF H+LVDE+QDTN LQ + L+ M V AVGDD Sbjct: 187 AELLLRTYELLSRNQPLREHYQSRFRHILVDEFQDTNDLQYKWLKLMAGQHNAVFAVGDD 246 Query: 287 AQAIYSFRGATVRNILDFPGLFTQPVRLLTLEQNYRSTQPILHASNAVIADAPERHVKHL 346 Q+IY+FRGA V N+ F F L+ LEQNYRS IL +N +IA+ +R K+L Sbjct: 247 DQSIYAFRGANVGNMQAFEQEF-HVQNLIKLEQNYRSHGHILDTANELIANNSKRLGKNL 305 Query: 347 WSDKPSSQKPQLVVIPDEAEQARWVANRILAHREEGLTLKSQAVLFRTATHSAALELELA 406 +D ++ ++ + ++A+W+ ++ EG A+L+R+ S LE L Sbjct: 306 RTDAGHGEQVRVYEATSDLQEAQWIIEQVKDLIAEGWARSEIAILYRSNAQSRVLEHALF 365 Query: 407 RRNIPFVKFGGLKFLESGHIKDLLALLRLAHNPSGRMAGLRALQL-IPGVGPATARKVLD 465 IP+ +GG +F E IK +A L+L N A LR + G+G K ++ Sbjct: 366 SSAIPYRVYGGQRFFERAEIKHAIAYLQLMDNLHNDSAFLRVVNFPTRGIGA----KAME 421 Query: 466 LMAVAADPADALLNFGAP---ANIALQWQAFADTYALMRRASASWPTDILAARDWYLPQL 522 + AA L P AF R + P + L L Sbjct: 422 SLQDAARQYGCSLYAAVPYVAGKAGSSLNAFVKLIEGARFETQHLPLPEMVKVVLDLSGL 481 Query: 523 QERYDDAQA---RAADIEQLAVLANGYASREKFLTELTLDPP------------EASSDF 567 Y + + R ++EQL A + S E + LD P A + Sbjct: 482 MTHYRNEKEGADRIENLEQLVSAATLFVSEEGY----GLDAPAFLGPQAQATAGAAITTA 537 Query: 568 SGPPLRDED------------YLTLSTIHSAKGQ-------------------EWRSVHI 596 G + D D +L+ +++ + Q E+ +V I Sbjct: 538 DGVEVLDADAPLVTIMSPLSAFLSHASLEAGDNQAQAGQDALQLMTVHSAKGLEFDAVFI 597 Query: 597 LNVVDGCIPSDLATGSEAEIAEERRLLYVAMTRARQYLHLVVPQRFYITQQTPQGDRHVN 656 + +G P + + E + EERRL+YVA+TRAR+ L + Q + QT Sbjct: 598 TGMEEGLFPHENSAKEEGGVEEERRLMYVAITRARKRLFMTFSQTRMLHGQTRY------ 651 Query: 657 GIRSRF-----------ITPRMLPHFEDSLWFSAETNGARTPMPES 691 +RSRF ++P++ H WF A A +PE+ Sbjct: 652 NMRSRFFDEMPEQSIKWLSPKIAQHH----WF-ANPKSAWDEVPEA 692 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,357,760 Number of extensions: 104922 Number of successful extensions: 333 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 323 Number of HSP's successfully gapped: 4 Length of query: 707 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 615 Effective length of database: 1,184,250 Effective search space: 728313750 Effective search space used: 728313750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)