BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0032.p (534 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312078|ref|YP_003776170.1| hypothetical protein Hsero_2767... 1086 0.0 gi|300310067|ref|YP_003774159.1| diguanylate cyclase GGDEF famil... 124 2e-29 gi|300311237|ref|YP_003775329.1| GGDEF domain-containing protein... 122 9e-29 gi|300310347|ref|YP_003774439.1| GGDEF domain transmembrane prot... 122 1e-28 gi|300311572|ref|YP_003775664.1| transmembrane protein [Herbaspi... 115 7e-27 >gi|300312078|ref|YP_003776170.1| hypothetical protein Hsero_2767 [Herbaspirillum seropedicae SmR1] Length = 534 Score = 1086 bits (2809), Expect = 0.0, Method: Compositional matrix adjust. Identities = 534/534 (100%), Positives = 534/534 (100%) Query: 1 MSKKPSTLTPVEIAREAFQRLGARRIAPTPDAYRIAYEEIQGDGPGASAESVLAAFATKL 60 MSKKPSTLTPVEIAREAFQRLGARRIAPTPDAYRIAYEEIQGDGPGASAESVLAAFATKL Sbjct: 1 MSKKPSTLTPVEIAREAFQRLGARRIAPTPDAYRIAYEEIQGDGPGASAESVLAAFATKL 60 Query: 61 SRQPGENGLLGTRLVRARENNDWAEFERQLIEFAEENWQNRPLGPQVQHGELVPIDLERE 120 SRQPGENGLLGTRLVRARENNDWAEFERQLIEFAEENWQNRPLGPQVQHGELVPIDLERE Sbjct: 61 SRQPGENGLLGTRLVRARENNDWAEFERQLIEFAEENWQNRPLGPQVQHGELVPIDLERE 120 Query: 121 FIRDSKKEKMLREMLARVLVFNLPSLLSTAPELANESRNSGQELKTAFSEQAMNEIIARL 180 FIRDSKKEKMLREMLARVLVFNLPSLLSTAPELANESRNSGQELKTAFSEQAMNEIIARL Sbjct: 121 FIRDSKKEKMLREMLARVLVFNLPSLLSTAPELANESRNSGQELKTAFSEQAMNEIIARL 180 Query: 181 KQLSFQIELKTDDMAQQQDLLMRLFQLLLENINGLLEQGSWLSSQIETVQSLIAGPLSTT 240 KQLSFQIELKTDDMAQQQDLLMRLFQLLLENINGLLEQGSWLSSQIETVQSLIAGPLSTT Sbjct: 181 KQLSFQIELKTDDMAQQQDLLMRLFQLLLENINGLLEQGSWLSSQIETVQSLIAGPLSTT 240 Query: 241 SLADATKNLKEVIYKQGLLKNTLSEEKVVVKNMMLTFVDRLSAMVSTTDNYQRTIRGFSQ 300 SLADATKNLKEVIYKQGLLKNTLSEEKVVVKNMMLTFVDRLSAMVSTTDNYQRTIRGFSQ Sbjct: 241 SLADATKNLKEVIYKQGLLKNTLSEEKVVVKNMMLTFVDRLSAMVSTTDNYQRTIRGFSQ 300 Query: 301 QISQAGNIADLNSVLSEIMTETQKAQEEATRSRDAMEDARQEVEKAEERIQTLEQQLLQM 360 QISQAGNIADLNSVLSEIMTETQKAQEEATRSRDAMEDARQEVEKAEERIQTLEQQLLQM Sbjct: 301 QISQAGNIADLNSVLSEIMTETQKAQEEATRSRDAMEDARQEVEKAEERIQTLEQQLLQM 360 Query: 361 GELVREDQLTGSLNRRGMDESLDREITNALRRDTPLCVALLDLDDFKRINDTHGHATGDE 420 GELVREDQLTGSLNRRGMDESLDREITNALRRDTPLCVALLDLDDFKRINDTHGHATGDE Sbjct: 361 GELVREDQLTGSLNRRGMDESLDREITNALRRDTPLCVALLDLDDFKRINDTHGHATGDE 420 Query: 421 VLVHMVQVIRETLRKLDVIARFGGEEFLVLMPETAPQDAVQIITRVQRELTKRIFMHESQ 480 VLVHMVQVIRETLRKLDVIARFGGEEFLVLMPETAPQDAVQIITRVQRELTKRIFMHESQ Sbjct: 421 VLVHMVQVIRETLRKLDVIARFGGEEFLVLMPETAPQDAVQIITRVQRELTKRIFMHESQ 480 Query: 481 RLLITFSAGVAVYHPGESQADLLKRADVALYKAKNAGKNRIVFADPPAPEQVGN 534 RLLITFSAGVAVYHPGESQADLLKRADVALYKAKNAGKNRIVFADPPAPEQVGN Sbjct: 481 RLLITFSAGVAVYHPGESQADLLKRADVALYKAKNAGKNRIVFADPPAPEQVGN 534 >gi|300310067|ref|YP_003774159.1| diguanylate cyclase GGDEF family protein [Herbaspirillum seropedicae SmR1] Length = 369 Score = 124 bits (312), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 72/182 (39%), Positives = 106/182 (58%), Gaps = 5/182 (2%) Query: 348 ERIQTLEQQLLQMGELVREDQLTGSLNRRGMDESLDREITNALRRDTPLCVALLDLDDFK 407 ER + L Q Q+ L D LTG NRR DE+L++E ++R TPL +A +D+D FK Sbjct: 181 ERTRDLAQANEQLRSLSLTDGLTGIANRRSFDETLEQEWRRSMRHGTPLMLAFIDVDWFK 240 Query: 408 RINDTHGHATGDEVLVHMVQVIRETLRKL-DVIARFGGEEFLVLMPETAPQDAVQIITRV 466 ND +GH GD VL + Q +R+++ ++ D +AR+GGEEF ++ +T Q ++ R+ Sbjct: 241 PYNDHYGHLAGDAVLRQVAQTLRQSIARVGDTVARYGGEEFAIIAADTDGQAGADMLRRM 300 Query: 467 QRELTKRIFMHESQRL-LITFSAGVAVYHPGESQA---DLLKRADVALYKAKNAGKNRIV 522 + H + IT SAGVA +PG+ A LL+ AD ALY+AK G+NR+V Sbjct: 301 CDAVHALAIAHAGSAMGRITISAGVASCNPGQPGAGLDGLLQAADAALYRAKMEGRNRVV 360 Query: 523 FA 524 A Sbjct: 361 LA 362 >gi|300311237|ref|YP_003775329.1| GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 585 Score = 122 bits (305), Expect = 9e-29, Method: Compositional matrix adjust. Identities = 64/156 (41%), Positives = 97/156 (62%), Gaps = 1/156 (0%) Query: 367 DQLTGSLNRRGMDESLDREITNALRRDTPLCVALLDLDDFKRINDTHGHATGDEVLVHMV 426 D LTG LNRR +++T A R L V L D+D FK+IND++GH GD +L + Sbjct: 416 DFLTGVLNRRAFFTHGQQQMTVAKRYGRELAVVLFDIDHFKQINDSYGHQAGDAILRGVA 475 Query: 427 QVIRETLRKLDVIARFGGEEFLVLMPETAPQDAVQIITRVQRELTKRIF-MHESQRLLIT 485 Q + + LRK+DV+AR+GGEEF++L+PE++ A +++ L F + +R+ +T Sbjct: 476 QSVSQLLRKVDVLARYGGEEFIILLPESSLLQAAAASEKLRAALAAGEFEIDGGRRIRVT 535 Query: 486 FSAGVAVYHPGESQADLLKRADVALYKAKNAGKNRI 521 S GVA + G L+K+AD+ALY+AK G+NR+ Sbjct: 536 ASFGVAAENGGPGLEHLIKQADIALYRAKKLGRNRV 571 >gi|300310347|ref|YP_003774439.1| GGDEF domain transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 334 Score = 122 bits (305), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 74/179 (41%), Positives = 109/179 (60%), Gaps = 6/179 (3%) Query: 346 AEERIQTLEQQLLQMGELVREDQLTGSLNRRGMDESLDREITNALRRDTPLCVALLDLDD 405 AE+R++ L ++L L D+LTG NR +DE LD EI A R L + LLD+D Sbjct: 148 AEQRVERLNEKL---ALLAVTDKLTGLANRMKLDEVLDNEINRADRYGKNLSLILLDIDH 204 Query: 406 FKRINDTHGHATGDEVLVHMVQVIRETLRKLDVIARFGGEEFLVLMPETAPQDAVQIITR 465 FK +ND GH+TGD VLV + +++R +R D+ R+GGEEFL+++PE +DA ++ + Sbjct: 205 FKEVNDNFGHSTGDAVLVRIAEILRANVRSSDLAGRWGGEEFLLILPECDMEDAARMAEK 264 Query: 466 VQRELTKRIFMHESQRLLITFSAGVAVYHPGESQADLLKRADVALYKAKNAGKNRIVFA 524 ++ + F+ +R T S GVA Y G + LL AD+ALY+AKN G+NR+ A Sbjct: 265 LRLLIENYNFVPVGRR---TCSFGVAAYQAGHDRTTLLTLADLALYRAKNTGRNRVEIA 320 >gi|300311572|ref|YP_003775664.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 395 Score = 115 bits (289), Expect = 7e-27, Method: Compositional matrix adjust. Identities = 64/159 (40%), Positives = 91/159 (57%), Gaps = 5/159 (3%) Query: 363 LVREDQLTGSLNRRGMDESLDREITNALRRDTPLCVALLDLDDFKRINDTHGHATGDEVL 422 L EDQLTG+LNRR + + + A+ R + L +A +D+D FK+IND HGH GD VL Sbjct: 230 LATEDQLTGALNRRELVRCGELLLARAVERKSVLALAFIDVDHFKQINDRHGHLAGDRVL 289 Query: 423 VHMVQVIRETLRKLDVIARFGGEEFLVLMPETAPQDAVQIITRVQRELTKRIFMHESQRL 482 + + ++ R DV+ R+GGEEF + P A QI R+ + + F H Sbjct: 290 AGIGATLAQSCRPGDVLGRYGGEEFCAVFPGVDHDQACQIGQRLLESVRTQRFEHGHP-- 347 Query: 483 LITFSAGVAVYHPGE--SQADLLKRADVALYKAKNAGKN 519 +T S G+AV PG S +L+ AD+ALY+AKN G+N Sbjct: 348 -VTISVGLAVLQPGNQRSWDELVHEADLALYRAKNEGRN 385 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.131 0.355 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,463,849 Number of extensions: 56878 Number of successful extensions: 330 Number of sequences better than 1.0e-08: 39 Number of HSP's gapped: 274 Number of HSP's successfully gapped: 39 Length of query: 534 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 444 Effective length of database: 1,193,720 Effective search space: 530011680 Effective search space used: 530011680 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)