BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0030.p (220 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312076|ref|YP_003776168.1| signal transduction histidine k... 401 e-113 gi|300310534|ref|YP_003774626.1| transmembrane sensor histidine ... 79 4e-16 gi|300311282|ref|YP_003775374.1| transmembrane sensor histidine ... 77 1e-15 gi|300311886|ref|YP_003775978.1| multi-sensor signal transductio... 69 2e-13 gi|300312939|ref|YP_003777031.1| transmembrane nitrate/nitrite s... 55 3e-09 >gi|300312076|ref|YP_003776168.1| signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 356 Score = 401 bits (1030), Expect = e-113, Method: Compositional matrix adjust. Identities = 215/220 (97%), Positives = 215/220 (97%) Query: 1 MSHPLPPPATAIHIREQERERIARDLHDDLGGVLAGVGMALGRLQEQLAAQGLAAPLAEA 60 MSHPLPPPATAIHIREQERERIARDLHDDLGGVLAGVGMALGRLQEQLAAQGLAAPLAEA Sbjct: 137 MSHPLPPPATAIHIREQERERIARDLHDDLGGVLAGVGMALGRLQEQLAAQGLAAPLAEA 196 Query: 61 DEARQLLQQAMASMHDIIDELHPPIVEFGLVDALQWQCRQIQREYRLHCQLDCPPALEQE 120 DEARQLLQQAMASMH IIDELHPPIVEFGLVDALQWQCRQIQREYRLHCQLDCPP LEQE Sbjct: 197 DEARQLLQQAMASMHSIIDELHPPIVEFGLVDALQWQCRQIQREYRLHCQLDCPPTLEQE 256 Query: 121 DRFLILGLLRILREAASNAARHGRASALRVGLRMRQARLEMQIADDGCGIAPLHAKEDAT 180 DRFLILGLLRILREAASNAARHGRASALRV LRMRQARLEMQIADDGCGIAPLHAKE A Sbjct: 257 DRFLILGLLRILREAASNAARHGRASALRVDLRMRQARLEMQIADDGCGIAPLHAKEVAP 316 Query: 181 RHGHGLRNMRERAQALGGELFIEAGQDGGVVIRTLIPVAR 220 RHGHGLRNMRERAQALGGELFIEAGQDGGVVIRTLIPVAR Sbjct: 317 RHGHGLRNMRERAQALGGELFIEAGQDGGVVIRTLIPVAR 356 >gi|300310534|ref|YP_003774626.1| transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 677 Score = 78.6 bits (192), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 59/211 (27%), Positives = 104/211 (49%), Gaps = 16/211 (7%) Query: 14 IREQERERIARDLHDDLGGVLAGVGMALGRLQEQLAAQGLAAPLAEADEARQ-----LLQ 68 +RE+E+ IAR+LHD++G L + M L + + L P E R+ L++ Sbjct: 468 VREEEKRAIARELHDNMGASLTALSMHLEGVYQIL-------PPDEKWADRKVRMQGLMK 520 Query: 69 QAMASMHDIIDELHPPIVE-FGLVDALQWQCRQIQREYRLHCQLDCPPALEQEDRFLILG 127 +A+ I EL P ++ FGL A+ Q ++ + C P + + + Sbjct: 521 SLVATTRRIQTELRPNTLDLFGLKAAISEQLDELHERTGIACHASLPDEDVEVGHEMEIA 580 Query: 128 LLRILREAASNAARHGRASALRVGLRMRQARLEMQIADDGCGIAPLHAKEDATRHGHGLR 187 + R+L+E +N +H +AS + V L + + + + + DDG GI P +++ +GLR Sbjct: 581 IYRMLQEMLNNVTKHAKASKVDVILDIDEDHVALTVRDDGVGI-PEERRDN--HKTYGLR 637 Query: 188 NMRERAQALGGELFIEAGQDGGVVIRTLIPV 218 +RERA GGE+ I + G +I +P+ Sbjct: 638 GLRERATFFGGEVDIRSTPGKGALITISLPI 668 >gi|300311282|ref|YP_003775374.1| transmembrane sensor histidine kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 464 Score = 76.6 bits (187), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 70/223 (31%), Positives = 108/223 (48%), Gaps = 29/223 (13%) Query: 15 REQERERIARDLHDDLGGVLAGVGMALGRLQEQLAAQGLAAPLAEADEARQLLQQAMASM 74 +E+ER R++RDLHD + L + +LQ + A L +A AR ++ + Sbjct: 246 QEEERARLSRDLHDGISQWLVSI-----KLQIEAALVRLNGTPEQAATARAGFEKTATQI 300 Query: 75 HDIIDE-------LHPPIVE-FGLVDALQWQCRQ--------IQREYRLHCQLDCPPALE 118 + ++ E L P I++ GL AL+ C + ++ + Q D L Sbjct: 301 NGVLAEVRRISHDLRPAILDDLGLAAALEHLCGEWRDAAGAGVEIVFENQAQADDGATLP 360 Query: 119 QEDRFLILGLLRILREAASNAARHGRASALRVGLRMRQARLEMQIADDGCGIAPLHAKED 178 + L RI +EA +N ARH AS ++V L L +Q+ D+G G L +D Sbjct: 361 D---VVNTVLFRIAQEALTNIARHAGASRIKVRLYQGHNLLMLQVQDNGHGF-DLGQVDD 416 Query: 179 ATRHGHGLRNMRERAQALGGELFIEAGQDG----GVVIRTLIP 217 +RHG GLRNMRER +A+GGEL I++ G V+ R +P Sbjct: 417 HSRHGIGLRNMRERIEAVGGELGIDSSPAGTSISAVIARASLP 459 >gi|300311886|ref|YP_003775978.1| multi-sensor signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 69.3 bits (168), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 56/210 (26%), Positives = 100/210 (47%), Gaps = 7/210 (3%) Query: 12 IHIREQERERIARDLHDDLGGVLAGVGMALGRLQEQLAAQGLAAPLAEADEARQLLQQAM 71 I ++E+ER+ +AR LHDD+G L + + L +++ + +G ++ + Sbjct: 265 IEVQERERQHLARGLHDDVGQSLTALKINLETIKKMGSLEG-----PSLQNGIDIVTAVL 319 Query: 72 ASMHDIIDELHPPIVE-FGLVDALQWQCRQIQREYRLHCQLDCPPALEQEDRFLILGLLR 130 A + + +L PP ++ GL+ AL+ Q + ++ + EQ + R Sbjct: 320 AQVRSLSLDLRPPQLDNLGLIAALRSYVEQKSKLANVNGWFESSGHDEQLHHDIENTAFR 379 Query: 131 ILREAASNAARHGRASALRVGLRMRQARLEMQIADDGCGIAPLHAKEDA-TRHGHGLRNM 189 I++EA +N RH R + V + + ++ + I DDG G A+ +A + GL NM Sbjct: 380 IVQEAVTNILRHARCQNIWVKIDRQHDQVYLSIRDDGRGFDIERARSNAISGTSFGLLNM 439 Query: 190 RERAQALGGELFIEAGQDGGVVIRTLIPVA 219 ERA +GG + I + G I +PVA Sbjct: 440 EERAVLVGGSMEITSAPGAGTEIVMHLPVA 469 >gi|300312939|ref|YP_003777031.1| transmembrane nitrate/nitrite sensor histidine kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 650 Score = 55.5 bits (132), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 56/193 (29%), Positives = 93/193 (48%), Gaps = 12/193 (6%) Query: 17 QERERIARDLHDDLGGVLAGVGMALGRLQEQLAAQGLAAPLAEADEARQLLQQAMASMHD 76 ER +A+ LHD + L + + + L++ LA L A R +Q+ + + Sbjct: 445 NERNLLAQGLHDSIAQGLNFLNLQVQMLEDSLARDRLDEIRDIAPLLRTGVQENYEDVRE 504 Query: 77 IIDELHPPIVEFGLVDALQWQCRQIQREYRLHCQL----DCPP-ALEQEDRFLILGLLRI 131 ++ + + L ++ ++QR+ L Q+ D PP A EQ+ L +L I Sbjct: 505 LLLNFRTRLQDSNLDSEMRKALDKLQRQSGLQGQITLTGDGPPLAPEQQ-----LQVLFI 559 Query: 132 LREAASNAARHGRASALRVGLRMRQARLEMQIADDGCGIAPLHAKEDATRHGHGLRNMRE 191 L+EA SN +H RA +++ + RQ M ++DDG G A+ H GLR M+E Sbjct: 560 LQEALSNIRKHARAQQVQISVDNRQD-FRMTVSDDGHGFTLESARAKGLEHV-GLRIMQE 617 Query: 192 RAQALGGELFIEA 204 RA+ LG L I++ Sbjct: 618 RAERLGANLEIDS 630 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.139 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 571,703 Number of extensions: 19554 Number of successful extensions: 95 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 91 Number of HSP's successfully gapped: 5 Length of query: 220 Length of database: 1,619,870 Length adjustment: 82 Effective length of query: 138 Effective length of database: 1,231,600 Effective search space: 169960800 Effective search space used: 169960800 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 129 (54.3 bits)