BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0026.p (725 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312072|ref|YP_003776164.1| hypothetical protein Hsero_2760... 797 0.0 gi|300312066|ref|YP_003776158.1| export protein [Herbaspirillum ... 423 e-119 gi|300312069|ref|YP_003776161.1| esterase/lipase/thioesterase fa... 255 1e-68 gi|300311322|ref|YP_003775414.1| O-antigen flippase [Herbaspiril... 98 2e-21 >gi|300312072|ref|YP_003776164.1| hypothetical protein Hsero_2760 [Herbaspirillum seropedicae SmR1] Length = 724 Score = 797 bits (2059), Expect = 0.0, Method: Compositional matrix adjust. Identities = 383/391 (97%), Positives = 384/391 (98%) Query: 335 ADLATPGMPTTGGRPDIGLLPGWNVLWLLSMDRRAREASLGTGDLAGSWSIHYRDRSSDR 394 A +A P MPTTGGRPDIGLLPGWNVLWLLSMDRRAREASLGTGDLAGSWSIHYRDRSSDR Sbjct: 334 AGVANPAMPTTGGRPDIGLLPGWNVLWLLSMDRRAREASLGTGDLAGSWSIHYRDRSSDR 393 Query: 395 PVSLARFPALTLLGQPGDARNPRTGKSEAFPPCQGCSNPNIADSAHMPAMNYLPYLLTGD 454 PVSLARFPALTLLGQPGDARNPRTGKSEAFPPCQGCSNPNIADSAHMPAMNYLPYLLTGD Sbjct: 394 PVSLARFPALTLLGQPGDARNPRTGKSEAFPPCQGCSNPNIADSAHMPAMNYLPYLLTGD 453 Query: 455 YYQLEELQFWATWAVFKSNPAYRGYAQGLVHRSQVRDQAWTLRSLGQAAYITPDDDALKK 514 YYQLEELQFWATWAVFKSNPAYRGYAQGLVHRSQVRDQAWTLRSLGQAAYITPDDDALKK Sbjct: 454 YYQLEELQFWATWAVFKSNPAYRGYAQGLVHRSQVRDQAWTLRSLGQAAYITPDDDALKK 513 Query: 515 ELQDIVKNNLDWYTAQYVNNSQANRLGIIVDNAIVYHGNTGIAPWMDDFFTSSIGYLNDL 574 ELQDIVKNNLDWYTAQYVNNSQANRLGIIVDNAIVY GNTGIAPWMDDFFTSSIGYLNDL Sbjct: 514 ELQDIVKNNLDWYTAQYVNNSQANRLGIIVDNAIVYQGNTGIAPWMDDFFTSSIGYLNDL 573 Query: 575 GYQEALPLLRYKAAFPVARMTHPSYCWIFGAAYSIPVRPDQNAPLYTDLGQAYQAMLAAN 634 GYQEALPLLRYKAAFPVARMTHP YCWIFGAAYSIPVRPDQNAPLYTDLGQAYQAMLAAN Sbjct: 574 GYQEALPLLRYKAAFPVARMTHPGYCWIFGAAYSIPVRPDQNAPLYTDLGQAYQAMLAAN 633 Query: 635 YRDNAPALAQTACASDPMAQLLKLRGGEMTGYSSQPAGFPSNLQPALAYSVDSDAPGARQ 694 YRDNAPALAQTACASD MAQLLKLRGGEMTGYSSQPAGFPSNLQPALAYSVDSDAPGARQ Sbjct: 634 YRDNAPALAQTACASDAMAQLLKLRGGEMTGYSSQPAGFPSNLQPALAYSVDSDAPGARQ 693 Query: 695 AWERFMWRSVKPDYGLGPQFSVMPRTAGKSN 725 AWERFM RSVKPDYGLGPQFSVMPRTAGKSN Sbjct: 694 AWERFMRRSVKPDYGLGPQFSVMPRTAGKSN 724 >gi|300312066|ref|YP_003776158.1| export protein [Herbaspirillum seropedicae SmR1] Length = 500 Score = 423 bits (1087), Expect = e-119, Method: Compositional matrix adjust. Identities = 220/238 (92%), Positives = 224/238 (94%), Gaps = 2/238 (0%) Query: 5 MHNDLRSKTVKAISHVGLGNAVGKLVSLSTTLVLARLLSPAEYGLMEIAMMLIAFIGFFN 64 MHNDLRSKTVKAISHVGLGNAVGKLVSLSTTLVLARLLSPAEYGLMEIAMMLIAFIGFFN Sbjct: 1 MHNDLRSKTVKAISHVGLGNAVGKLVSLSTTLVLARLLSPAEYGLMEIAMMLIAFIGFFN 60 Query: 65 EIGLTTAIVQRPQLSQEQVNGCFLIAMGCSVVLFLLTLLAAGPLAGFFRQPQLQPMISTL 124 EIGLTTAIVQRPQLSQEQVNGCFLIAMGCSVVLFLLTLLAAGPLAGFFRQPQLQPMISTL Sbjct: 61 EIGLTTAIVQRPQLSQEQVNGCFLIAMGCSVVLFLLTLLAAGPLAGFFRQPQLQPMISTL 120 Query: 125 AVGFIMGAAGSVPEAFLRKQMQFKAMAGITILGIVLQSAVSLGLALAGMGVWSLVWGSLV 184 AVGFIMGAAGSVPEAFLRKQMQFKAMAGITILGIVLQSAVSLGLALAGMGVWSLVWGSLV Sbjct: 121 AVGFIMGAAGSVPEAFLRKQMQFKAMAGITILGIVLQSAVSLGLALAGMGVWSLVWGSLV 180 Query: 185 ATATYSGGYFLLSPWRPRGRYGVREAVELVLFGLCDGASAAL--LHASADARVRAMVL 240 ATATYSGGYFLLSPWRPRGRYGVREAVELVLFGL S L+++AD + VL Sbjct: 181 ATATYSGGYFLLSPWRPRGRYGVREAVELVLFGLHVITSRVFWYLYSNADRAIVGRVL 238 >gi|300312069|ref|YP_003776161.1| esterase/lipase/thioesterase family protein [Herbaspirillum seropedicae SmR1] Length = 268 Score = 255 bits (651), Expect = 1e-68, Method: Compositional matrix adjust. Identities = 127/129 (98%), Positives = 129/129 (100%) Query: 213 LVLFGLCDGASAALLHASADARVRAMVLVNPWARTEAGQAQAYLKHYYRARLTSPDLWNK 272 +VL+GLCDGASAALLHASADARVRAMVLVNPWARTEAGQAQAYLKHYYRARLTSPDLWNK Sbjct: 94 VVLWGLCDGASAALLHASADARVRAMVLVNPWARTEAGQAQAYLKHYYRARLTSPDLWNK 153 Query: 273 ILRGQFNAVAALRSLLGQWRRAASPPAATPELLPIRLQQAYERFRGPVLLILSGADLTAR 332 ILRGQFNAVAALRSLLGQWRRAASPPAATPELLPIRLQQAYERFRGPVLLILSGADLTAR Sbjct: 154 ILRGQFNAVAALRSLLGQWRRAASPPAATPELLPIRLQQAYERFRGPVLLILSGADLTAR 213 Query: 333 EFADLATPG 341 EFADLATPG Sbjct: 214 EFADLATPG 222 >gi|300311322|ref|YP_003775414.1| O-antigen flippase [Herbaspirillum seropedicae SmR1] Length = 476 Score = 98.2 bits (243), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 58/189 (30%), Positives = 103/189 (54%), Gaps = 2/189 (1%) Query: 22 LGNAVGKLVSLSTTLVLARLLSPAEYGLMEIAMMLIAFIGFFNEIGLTTAIVQRPQLSQE 81 L V + + + +L+RLL+P+EYGL+ +A + + F ++G A++Q+ L++E Sbjct: 13 LSQMVRIALQMVSIFILSRLLAPSEYGLLAMATVAVNFASLLRDMGTGAAVIQKENLTRE 72 Query: 82 QVNGCFLIAMGCSVVLFLLTLLAAGPLA-GFFRQPQLQPMISTLAVGFIMGAAGSVPEAF 140 N F + +G V + L L+A P+ +FR P L P++ +A+ F + ++ +V A Sbjct: 73 TTNTVFWLNVGLGVSI-ALVLIAISPMVEDYFRTPHLAPVLCLMALSFPLTSSCAVHSAL 131 Query: 141 LRKQMQFKAMAGITILGIVLQSAVSLGLALAGMGVWSLVWGSLVATATYSGGYFLLSPWR 200 L ++ F+ +A I I+ L ++ LA G GV+SLVW +LV+ + +L S WR Sbjct: 132 LERESNFRTLARIEIVSAALALVTAVTLAFTGFGVYSLVWQTLVSAMLSALQLWLASRWR 191 Query: 201 PRGRYGVRE 209 P + E Sbjct: 192 PEKIWSRSE 200 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.418 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,405,788 Number of extensions: 107161 Number of successful extensions: 334 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 334 Number of HSP's successfully gapped: 4 Length of query: 725 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 633 Effective length of database: 1,184,250 Effective search space: 749630250 Effective search space used: 749630250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)