BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0016.p (539 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312041|ref|YP_003776133.1| acyl-CoA synthetase/AMP-acid li... 1070 0.0 gi|300311658|ref|YP_003775750.1| non-ribosomal peptide synthetas... 156 4e-39 gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligas... 140 3e-34 gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligas... 132 9e-32 gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [H... 119 4e-28 >gi|300312041|ref|YP_003776133.1| acyl-CoA synthetase/AMP-acid ligase II protein [Herbaspirillum seropedicae SmR1] Length = 539 Score = 1070 bits (2767), Expect = 0.0, Method: Compositional matrix adjust. Identities = 535/539 (99%), Positives = 538/539 (99%) Query: 1 VTPTLIHDTLLASAQARPQATALRYRGQQLDYAGLAAGVEQVAAALLALGLGPGERVAVY 60 +TPTLIHDTLLASAQARPQATALRYRGQQLDYAGLAAGVEQVAAALLALGLGPGERVAVY Sbjct: 1 MTPTLIHDTLLASAQARPQATALRYRGQQLDYAGLAAGVEQVAAALLALGLGPGERVAVY 60 Query: 61 LEKRMETVLALFGAAAAGLVFVPINPLLKPAQVSHILQDCSVRVLVTSEPRHPALADRLS 120 LEKRMETVLALFGAAAAGLVFVPINPLLKPAQVSHILQDC+VRVLV+SEPRHPALADRLS Sbjct: 61 LEKRMETVLALFGAAAAGLVFVPINPLLKPAQVSHILQDCAVRVLVSSEPRHPALADRLS 120 Query: 121 QCPALQTVVLVDAAAAAPATPAPPAPLGRLATLDWNAFLQAAPGPGSRRAHRRIDADIAA 180 QCPALQTVVLVDAAAAAPATPAPPAPLGRLATLDWNAFLQAAPGPGSRRAHRRIDADIAA Sbjct: 121 QCPALQTVVLVDAAAAAPATPAPPAPLGRLATLDWNAFLQAAPGPGSRRAHRRIDADIAA 180 Query: 181 ILYTSGSTGQPKGVVLSHRNLVAGAQSVAGYLGNHPQDRLLAVLPLSFDYGLSQLSTAFL 240 ILYTSGSTGQPKGVVLSHRNLVAGAQSVAGYLGNHPQDRLLAVLPLSFDYGLSQLSTAFL Sbjct: 181 ILYTSGSTGQPKGVVLSHRNLVAGAQSVAGYLGNHPQDRLLAVLPLSFDYGLSQLSTAFL 240 Query: 241 SGASVTLLNYLAPRDILHAVASEGITGLAGVPPLWQQLAGLPWPQGCSLRYLTNSGGALP 300 SGASVTLLNYLAPRDILHAVASEGITGLAGVPPLWQQLAGLPWPQGCSLRYLTNSGGALP Sbjct: 241 SGASVTLLNYLAPRDILHAVASEGITGLAGVPPLWQQLAGLPWPQGCSLRYLTNSGGALP 300 Query: 301 RSVLAQLRRALPQAQVFLMYGLTEAFRSTYLPPAELDRRPDSIGRAIPNAEIMVVRPDGS 360 RSVLAQLRRALPQAQVFLMYGLTEAFRSTYLPPAELDRRPDSIGRAIPNAEIMVVRPDGS Sbjct: 301 RSVLAQLRRALPQAQVFLMYGLTEAFRSTYLPPAELDRRPDSIGRAIPNAEIMVVRPDGS 360 Query: 361 PCAPGEVGELVHRGALVAQGYWNDPARTAERFRPAPGREAALGTPELAVWSGDSVRMDEQ 420 PCAPGEVGELVHRGALVAQGYWNDPARTAERFRPAPGREAALGTPELAVWSGDSVRMDEQ Sbjct: 361 PCAPGEVGELVHRGALVAQGYWNDPARTAERFRPAPGREAALGTPELAVWSGDSVRMDEQ 420 Query: 421 GYLYFVGRADDMIKVSGYRISPHEVEEVLHATGLADQFAVVGVAHPQQGQAVVAVAPSSC 480 GYLYFVGRADDMIKVSGYRISPHEVEEVLHATGLADQFAVVGVAHPQQGQAVVAV PSSC Sbjct: 421 GYLYFVGRADDMIKVSGYRISPHEVEEVLHATGLADQFAVVGVAHPQQGQAVVAVVPSSC 480 Query: 481 TASLEAVQQACRAHLPSYMLPAALHRWPGELPRSPNGKLDRKLMQQTLADHFMKADHAR 539 TASLEAVQQACRAHLPSYMLPAALHRWPGELPRSPNGKLDRKLMQQTLADHFMKADHAR Sbjct: 481 TASLEAVQQACRAHLPSYMLPAALHRWPGELPRSPNGKLDRKLMQQTLADHFMKADHAR 539 >gi|300311658|ref|YP_003775750.1| non-ribosomal peptide synthetase [Herbaspirillum seropedicae SmR1] Length = 9160 Score = 156 bits (394), Expect = 4e-39, Method: Compositional matrix adjust. Identities = 158/531 (29%), Positives = 231/531 (43%), Gaps = 59/531 (11%) Query: 18 PQATALRYRGQQLDYAGLAAGVEQVAAALLALGLGPGERVAVYLEKRMETVLALFGAAAA 77 P A AL Q L Y L ++A L++LG+G +RV V +E+ + V+ L A Sbjct: 8276 PDAIALVLEEQSLSYGELNVRANRLAHHLMSLGVGAEQRVGVAVERSFDMVVGLLAVMKA 8335 Query: 78 GLVFVPINPLLKPAQVSHILQDCSVRVLVTSEPRHPALADRLS---QCPALQTVVLVDAA 134 G +VP++ P ++++++ D +RVL+T LA + +C AL L Sbjct: 8336 GAAYVPLDLDYPPERLAYMVADSRIRVLLTHARARRLLAAQAQPGLRCLALDDADLAAQL 8395 Query: 135 AAAPATPAPPAPLGRLATLDWNAFLQAAPGPGSRRAHRRIDADIAAILYTSGSTGQPKGV 194 A PA + + AP +A ++YTSGSTGQPKGV Sbjct: 8396 AGLPAH---------------DPVVAVAP------------QQMAYVIYTSGSTGQPKGV 8428 Query: 195 VLSHRNLVAGAQSVAGYLGNHPQDRLLAVLPLSFDYGLSQLSTAFLSGASVTLL--NYLA 252 +SH LV Q+ A PQ R L + SFD + T +SGA++ L + Sbjct: 8429 AVSHGPLVMHCQATAALYEMTPQWRELQFMSFSFDGAHERWITTLISGAALVLRGPEMWS 8488 Query: 253 PRDILHAVASEGITGLAGVPPLWQQLAGLPWPQGCS--LRYLTNSGGALPRSVLAQLRRA 310 A+ + ++ P QLA G + L G A+PR+ ++RR Sbjct: 8489 IEQSWQALHQQRVSNAVFPPAYLGQLAQWGEQHGDAPPLELYVFGGEAMPRASFERVRRH 8548 Query: 311 LPQAQVFLMYGLTEA------FRSTYLPPAELDRRPDSIGRAIPNAEIMVVRPDGSPCAP 364 L + YG TE ++ T + D IG+ + ++ P AP Sbjct: 8549 LAPRLLINGYGPTETVVTPLIWKGTSAAGVDFDGPFVPIGKPVGRRNAYILDACLQPVAP 8608 Query: 365 GEVGELVHRGALVAQGYWNDPARTAERFRPAPGREAALGTPELAVW-SGDSVRMDEQGYL 423 GE GEL G +A+GY PA++AERF P G P ++ +GD R G + Sbjct: 8609 GEAGELYIGGDGLARGYLERPAQSAERFVADP-----FGEPGARMYRTGDLARWLPDGNV 8663 Query: 424 YFVGRADDMIKVSGYRISPHEVEEVLHAT-GLADQFAVV---GVAHPQQGQAVVAVAPSS 479 ++GR D +K+ G+RI E+E +L G+A+ VV G Q V V Sbjct: 8664 EYLGRLDHQVKIRGFRIELGEIEALLRRQPGVAEAAVVVRSDGQGEETVRQLVAYVGAPQ 8723 Query: 480 CTASLEAVQQ--------ACRAHLPSYMLPAALHRWPGELPRSPNGKLDRK 522 A+ E Q+ A RA LP YMLPA LP + NGKLDRK Sbjct: 8724 AVAAPETAQRDFSDSLRAALRAGLPEYMLPAHFVLL-AALPVTHNGKLDRK 8773 Score = 146 bits (369), Expect = 4e-36, Method: Compositional matrix adjust. Identities = 160/546 (29%), Positives = 241/546 (44%), Gaps = 80/546 (14%) Query: 12 ASAQARPQATALRYRGQQLDYAGLAAGVEQVAAALLALGLGPGERVAVYLEKRMETVLAL 71 A A A P A A+ QQ+ YA L ++A LL G+G +R+ + + +E ++ L Sbjct: 5669 AQAAAIPDAVAVVCGPQQMSYAQLNRAANRLAHRLLQQGVGLEDRIGLAAPRSLEMIVGL 5728 Query: 72 FGAAAAGLVFVPINPLLKPAQVSHILQDCSVRVLVTSEPRHPALADRLSQCPALQTVVLV 131 AG +VP++P ++++++Q C RHP + R Sbjct: 5729 LAILKAGAAYVPLDPDYPADRLAYMVQACR---------RHPGVDGR------------- 5766 Query: 132 DAAAAAPATPAPPAPLGRLATLDWNAFLQAAPGPGSRRAHRRID-------ADIAAILYT 184 A + P P R A W + P P + A + ++A I+YT Sbjct: 5767 ----ARVSLPGP-----RKACAHWRS-----PRPWADDAAPAHNPGVVLHPDNLAYIIYT 5812 Query: 185 SGSTGQPKGVVLSHRNLVAGAQSVAGYLGNHPQDRLLAVLPLSFDYGLSQLSTAFLSGAS 244 SGSTG+PKG LSH N++ A + +D +FD+ + ++ A G Sbjct: 5813 SGSTGRPKGAQLSHANVLRLLGQTAAWFDFGREDVWTLFHSYAFDFSVWEIFGALCYGGR 5872 Query: 245 VTLLNYL---APRDILHAVASEGITGLAGVPPLWQQLAGLPW-----PQGCSLRYLTNSG 296 + ++ + +P D L + + +T L P ++QL LP + +LR + G Sbjct: 5873 LVVVPFAVSRSPEDFLALLVEQRVTVLNQTPSAFRQLLQLPALRQLPSEALALRTVIFGG 5932 Query: 297 GALP----RSVLAQLRRALPQAQVFLMYGLTE-AFRSTY--LPPAELDRRPDSIGRAIPN 349 AL R + Q P + MYG+TE TY L A+LD IG AIP+ Sbjct: 5933 EALEPEMLRPWITQFGDQRP--ALINMYGITETTVHVTYRRLLRADLDASRSPIGAAIPD 5990 Query: 350 AEIMVVRPDGSPCAPGEVGELVHRGALVAQGYWNDPARTAERFRPAPGREAALGTPELAV 409 + V+ + G GEL GA +A+GY N A +AERF P A L Sbjct: 5991 LGLYVLDQQLNLAPAGVPGELYVSGAGLARGYLNRAALSAERFVANPFARAGEEGSRLYR 6050 Query: 410 WSGDSVRMDEQGYLYFVGRADDMIKVSGYRISPHEVEEVL-------HATGLADQFA--- 459 +GD VR G + ++GR D +K+ G+RI E+E L AT + Q A Sbjct: 6051 -TGDLVRWRHDGQMDYLGRIDQQVKIRGFRIELGEIEAQLLAQPGVGAATVMVRQDAATP 6109 Query: 460 -VVGVAHPQQGQAVVAVAPSSCTASLEAVQQACRAHLPSYMLPAALHRWPGELPRSPNGK 518 +VG PQ G +AP + A++ A A LP YM+PAAL P LP + NGK Sbjct: 6110 RLVGYLSPQAG-----IAPEALDTG--ALRTALGAVLPDYMVPAALVMLPA-LPLNINGK 6161 Query: 519 LDRKLM 524 +DRK + Sbjct: 6162 VDRKAL 6167 Score = 145 bits (367), Expect = 6e-36, Method: Compositional matrix adjust. Identities = 159/527 (30%), Positives = 230/527 (43%), Gaps = 39/527 (7%) Query: 6 IHDTLLASAQARPQATALRYRGQQLDYAGLAAGVEQVAAALLALGLGPGERVAVYLEKRM 65 +H A RP A AL+ G Y L A +A L A G+GP VA+ E+ Sbjct: 2646 VHRLFEQQASRRPDAIALQADGAHWTYGQLNAAANALARRLQAAGVGPDTVVALLAERSA 2705 Query: 66 ETVLALFGAAAAGLVFVPINPLLKPAQVSHILQDCSVRVLVTSEPRHPALADRLSQCPAL 125 V AL AG ++P++P +++ +L+D L++ H L RL AL Sbjct: 2706 AMVTALLAVLKAGGAYLPLDPDYPAERLAWMLEDARPVCLLS----HRGLHRRL----AL 2757 Query: 126 QTVVLVDAAAAAPATPAPPAPLGRLATLDWNAFLQAAPGPGSRRAHRRIDADIAAILYTS 185 +L++ P + P +N Q P P H +A ++YTS Sbjct: 2758 PDTLLLELEDQLPGSQRGDEP-------QYNP--QHNPQPPLHGEH------LAYVIYTS 2802 Query: 186 GSTGQPKGVVLSHRNLVAGAQSVAGYLGNHPQDRLLAVLPLSFDYGLSQLSTAFLSGASV 245 GSTG+PKG SHR L + + D +L P FD + + ++GA + Sbjct: 2803 GSTGRPKGAGNSHRALYNRLRWMQQAYALQEGDAVLQKTPFGFDVSVWEFLWPLMTGARL 2862 Query: 246 TLL---NYLAPRDILHAVASEGITGLAGVPPLWQQLAGLPWPQGC-SLRYLTNSGGALPR 301 + + P ++ + G++ L VP + Q C SLR + SG ALP Sbjct: 2863 VMAPPGAHRDPAELAALIREHGVSTLHFVPSMLQAFLASGQAAACTSLRQVICSGEALPA 2922 Query: 302 SVLAQLRRALPQAQVFLMYGLTEA-FRSTYLPPAELDRRPDSIGRAIPNAEIMVVRPDGS 360 Q LPQA + +YG TEA T+ DR P IG+ I +V+ D + Sbjct: 2923 EARDQFFALLPQAALHNLYGPTEAAIDVTHWTCQHGDRGPVPIGQPISGLRAVVLDADLN 2982 Query: 361 PCAPGEVGELVHRGALVAQGYWNDPARTAERFRPAPGREAALGTPELAVWSGDSVRMDEQ 420 G GEL G +A+GY P TAERF +P G E +GD VR E Sbjct: 2983 VLPCGVAGELYLGGIGLARGYLGRPGLTAERFVASP-----FGQGERLYRTGDLVRWRED 3037 Query: 421 GYLYFVGRADDMIKVSGYRISPHEVEEVLHATGLADQFAVVGVAHPQQGQAVVAVAPSSC 480 G + ++GR D +K+ G RI E+E L A ++ V VAH V+A +S Sbjct: 3038 GQIDYLGRLDHQVKIRGLRIELGEIEAQLLALDEVEEAVV--VAHEHGAGPVLAGYVASR 3095 Query: 481 TASLE---AVQQACRAHLPSYMLPAALHRWPGELPRSPNGKLDRKLM 524 + ++QA A LP YM+PA L LP + NGK+DRK + Sbjct: 3096 RWQRQDAATLKQALAARLPDYMVPAVLTL-LPTLPLNANGKIDRKAL 3141 Score = 137 bits (344), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 173/552 (31%), Positives = 238/552 (43%), Gaps = 83/552 (15%) Query: 3 PTLIHDTLLA-SAQARPQATALRYRGQQLDYAGLAAGVEQVAAALLALGLGPGERVAVYL 61 PTL ++A A A+PQ+ AL G LDYAGL VE +A AL +LG+ PG+ VA+ L Sbjct: 4561 PTLTVAQMIAHRAAAQPQSPALAAGGAVLDYAGLERRVEHLAHALRSLGVAPGDIVALQL 4620 Query: 62 EKRMETVLALFGAAAAGLVFVPINPLLKPAQVSHILQDCSVRVLVTSEPRHPALADRLSQ 121 + E VLAL G ++P++ A+ ILQ V +LV Sbjct: 4621 PRCAELVLALLATWRCGAAYLPLDTDSPVARRQQILQAAKVHLLVMEA------GQSAEG 4674 Query: 122 CPALQTVVLVDAAAAAPATPAPPAPLGRLATLDWNAFLQAAPGPGSRRAHRRIDADIAAI 181 PA T V D A +P +AP PGS R+DA A + Sbjct: 4675 LPADCTAVAWDELANSPVQA-------------------SAPLPGS-----RMDA-TAYV 4709 Query: 182 LYTSGSTGQPKGVVLSHRNLVAGAQSVAGYLGNHPQDRLLAVLPLSFDYGLSQLSTAFLS 241 L+TSGSTG PKGV++ H L A Q V L ++ D G + L A + Sbjct: 4710 LFTSGSTGAPKGVLIGHAQLAAYVQGVTQALRLDACRHWALTGTVAADLGNTALFGALAN 4769 Query: 242 GASVTLLNYLAPRD---ILHAVASEGITGLAGVPPLWQQLAGLPWPQGCSLRYLTNSGGA 298 GA + + + RD + GI L VP L P P + L G A Sbjct: 4770 GACLHVASAEDMRDGERFAKFLRQHGIDALKIVPSHLDALLETPAPYLPAT--LVLGGEA 4827 Query: 299 LPRSVLAQLRRALPQAQVFLMYGLTEA------FRSTYLPPAELDRRPDS---IGRAIPN 349 + LA++R PQ +VF YG TE R+ AE + P + + A+P+ Sbjct: 4828 TAPAQLARIRALAPQCRVFNHYGPTECTVGVLVHRAATEAEAEAEADPGASLPLTEALPH 4887 Query: 350 AEIMVVRPDGSPCAPGEVGELVHRGALVAQGYWNDPARTAERFRPAPGREAALGTPEL-- 407 A + V+ P G +G+L GA +A+GY + REA + P L Sbjct: 4888 AYVRVLDAQLRPTPAGAIGQLWLGGAQLARGYLD----------AQQDREAFIDDPWLPG 4937 Query: 408 --AVWSGDSVRMDEQGYLYFVGRADDMIKVSGYRISPHEVEEVLHATGLADQFAVVGVAH 465 SGD + QG + GRAD +KV G+RI P E+E L Sbjct: 4938 RRLYRSGDLAVLLPQGGVRLAGRADHQVKVRGFRIEPGEIEAALR-------------TQ 4984 Query: 466 PQQGQAVVAVAP----SSCTA-----SLEAVQQACRAHLPSYMLPAALHRWPGELPRSPN 516 P QA+V AP ++C A +L A + A LP+ M P+ P ELPR N Sbjct: 4985 PGVRQALVLAAPDGALAACLAADHGQALPAWRAALAEVLPAQMQPSRWLVLP-ELPRLGN 5043 Query: 517 GKLDRKLMQQTL 528 GK+DRK + + L Sbjct: 5044 GKVDRKALLEQL 5055 Score = 134 bits (338), Expect = 1e-32, Method: Compositional matrix adjust. Identities = 153/507 (30%), Positives = 219/507 (43%), Gaps = 37/507 (7%) Query: 23 LRYRGQQLDYAGLAAGVEQVAAALLALGLGPGERVAVYLEKRMETVLALFGAAAAGLVFV 82 L Y LDY L ++A LLA G GP V V + E V+AL AG ++ Sbjct: 7216 LLYEDLVLDYRSLNQQANRLAHRLLAEGAGPERIVGVAARRAPEMVVALLAVLKAGAAYL 7275 Query: 83 PINPLLKPAQVSHILQDCSVRVLVTSEPRHPALADRLSQCPALQTVVLVDAAAAAPATPA 142 P++P L PA+++ ++ D + +L+T AL L+ PA V+ +DA A Sbjct: 7276 PLDPDLPPARLADMMADSGLSLLLT----QTALRAELT-APAAVRVLELDAPGQPWAEGM 7330 Query: 143 PPAPLGRLATLDWNAFLQAAPGPGSRRAHRRIDADIAAILYTSGSTGQPKGVVLSHRNLV 202 P D + ++ AP +A ++YTSGSTG+PKG H +L Sbjct: 7331 P----------DHDPGIEVAPD------------GLAYVIYTSGSTGKPKGAGNRHVSLH 7368 Query: 203 AGAQSVAGYLGNHPQDRLLAVLPLSFDYGLSQLSTAFLSGASVTLLNYLAPRD--ILHA- 259 + P + +L P FD + + ++GA + L RD +L A Sbjct: 7369 NRLAWMQEAYPIGPGNTVLQKTPFGFDVSVWEFFWPLMTGARLALAAPDEHRDPALLAAR 7428 Query: 260 VASEGITGLAGVPPLWQQLAGLPWPQGC-SLRYLTNSGGALPRSVLAQLRRALPQAQVFL 318 + + L VP + Q P C SLR + SG ALP + Q LPQ + Sbjct: 7429 IRRHAVDTLHFVPSMLQGFVAHPDSAQCHSLRQVICSGEALPAELQEQAFARLPQIALHN 7488 Query: 319 MYGLTEAFRSTYLPPAELDRRPDSIGRAIPNAEIMVVRPDGSPCAPGEVGELVHRGALVA 378 +YG TEA R IGR I + V+ +P G GEL G +A Sbjct: 7489 LYGPTEAAIDVTHWTCRPGERSVPIGRPISHTAAYVLDGGLNPLPHGIAGELYLGGIGLA 7548 Query: 379 QGYWNDPARTAERFRPAPGREAALGTPELAVWSGDSVRMDEQGYLYFVGRADDMIKVSGY 438 +GY P +AERF P AA G+ +GD VR G + ++GR D +K+ G Sbjct: 7549 RGYLGRPGLSAERFVADPF--AADGSRMYR--TGDLVRRRADGEIEYLGRTDHQVKIRGL 7604 Query: 439 RISPHEVEEVLHATGLADQFAVVGVAHPQQGQAVVAVAPS-SCTASLEAVQQACRAHLPS 497 RI E+E L A VV + P + +V A EA++ A LP Sbjct: 7605 RIELGEIEAQLLAQPGVAAAVVVALVEPGRPARLVGYAADPGGMLEAEALRAALLQSLPD 7664 Query: 498 YMLPAALHRWPGELPRSPNGKLDRKLM 524 YM+P L P LP + NGK+DRK + Sbjct: 7665 YMVPQRLILLPA-LPLNANGKVDRKAL 7690 Score = 115 bits (287), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 146/522 (27%), Positives = 222/522 (42%), Gaps = 52/522 (9%) Query: 14 AQARPQATALRYRGQQLDYAGLAAGVEQVAAALLALGLGPGERVAVYLEKRMETVLALFG 73 A A+P A ALR Q + + G + +A A G GP RVA+Y + ++ V + Sbjct: 1154 ALAQPDAVALRCGAQFISTSSCCNGSMRWRSAWHAAGAGPESRVALYAGRDIDFVCGMLA 1213 Query: 74 AAAAGLVFVPINPLLKPAQVSHILQDCSVRVLVTSEPRHPALADRLSQCPALQTVVLVDA 133 G +VP++P L +++ LQD R ++ PA L+Q ++ ++A Sbjct: 1214 VLDTGAAYVPLDPQLPAERLAWQLQDSGARWVLHEGQSAPAW---LAQAGV--PLLSLEA 1268 Query: 134 AAAAPATPAPPAPLGRLATLDWNAFLQAAPGPGSRRAHRRIDADIAAILYTSGSTGQPKG 193 A APA+P P P+ A A ++YTSGSTG+PKG Sbjct: 1269 AGTAPASPWTPFPVH--------------------------PAQAAYLIYTSGSTGRPKG 1302 Query: 194 VVLSHRNLVAGAQSVAGYLGNHPQDRLLAVLP-LSFDYGLSQLSTAFLSGASVTLLNY-- 250 V+++ L +V L R +A++ ++ D G + L A SG + L+ + Sbjct: 1303 VLVTRGGLANYVAAVLEKLALPAAARSMAMISTVAADLGHTTLYGALCSGRQLHLITHDL 1362 Query: 251 -LAPRDILHAVASEGITGLAGVPPLWQQLAGLPWPQGCSLRY-LTNSGGALPRSVLAQLR 308 P + + L VP Q L P+ R+ L G A +L ++ Sbjct: 1363 AFDPDGFAAYLHEHAVDVLKIVPSHLQALLHAARPEQVLPRHTLVLGGEATGWPLLERIA 1422 Query: 309 RALPQAQVFLMYGLTEAFRSTYLPPAELDRRPDS---IGRAIPNAEIMVVRPDGSPCAPG 365 P +V YG +E + A R + +G A+ + V+ G Sbjct: 1423 ALQPACRVLNHYGPSETTVGVLVQEAASASRRAAVLPVGSALVGVQAWVLDRQLRRAPVG 1482 Query: 366 EVGELVHRGALVAQGYWNDPARTAERFRPAPGREAALGTPELAVWSGDSVRMDEQGYLYF 425 VGEL GA +A+GY A TAERF P + + SGD R G + F Sbjct: 1483 AVGELYLGGAGLARGYHGRAALTAERFVAHP-----FASGQRLYRSGDRARYLGDGSIEF 1537 Query: 426 VGRADDMIKVSGYRISPHEV-EEVLHATGLADQFAVVGVAHPQQGQAVVAVAPSSCTASL 484 +GR DD +K+ G+R+ P EV +++L G+ D AV+ + Q + C A Sbjct: 1538 LGRIDDQVKIRGHRVEPGEVRQQLLRLAGVVDA-AVLALPAADDAQRLQLHGFIVCAAGA 1596 Query: 485 EAVQQACR----AHLPSYMLPAALHRWPGELPRSPNGKLDRK 522 A R A LP Y+LP L P LP + NGKLDR+ Sbjct: 1597 VADPAGWRATLAADLPDYLLP-TLTPVPA-LPLTANGKLDRQ 1636 Score = 56.6 bits (135), Expect = 5e-09, Method: Compositional matrix adjust. Identities = 61/202 (30%), Positives = 86/202 (42%), Gaps = 33/202 (16%) Query: 337 DRRPDSIGRAIPNAEIMVVRPDGSPCAPGEVGELVHRGALVAQGYWNDPARTAERFRPAP 396 D R + G P+ E+ ++ DG G VGE+ G V QGYW P +A F Sbjct: 375 DTRLVACGDIAPHHEVRIIE-DGRVQQDGAVGEIEVCGPSVTQGYWQRPQESAAAFVERD 433 Query: 397 GREAALGTPELAVW--SGDSVRMDEQGYLYFVGRADDMIKVSGYRISPHEVEEVLHA--- 451 GR W +GD + G LY GR D+I V G + P ++E + Sbjct: 434 GRR----------WLRTGD-LGFLRDGQLYIAGRRKDLIIVRGQNVYPQDIERAVEDQVE 482 Query: 452 ---TGLADQFAV-------VGV-AHPQQGQAVVAVAPSSCTASLEAVQQACRAHLPSYML 500 G FAV +GV A +G +A + +A EAV QA L + + Sbjct: 483 AVRKGRVAAFAVNTGDGEGIGVAAEISRGMQKLAAPGAIASAIAEAVAQAS---LEAPQV 539 Query: 501 PAALHRWPGELPRSPNGKLDRK 522 L+ PG LP++ +GKL R Sbjct: 540 VILLN--PGGLPKTSSGKLQRS 559 >gi|300313157|ref|YP_003777249.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 566 Score = 140 bits (353), Expect = 3e-34, Method: Compositional matrix adjust. Identities = 140/504 (27%), Positives = 214/504 (42%), Gaps = 54/504 (10%) Query: 54 GERVAVYLEKRMETVLALFGAAAAGLVFVPINPLLKPAQVSHILQD-------------C 100 G RVA+ + ++ +A+ AG V +NPL ++ H L+D C Sbjct: 73 GARVAIMMPNVLQYPIAIAAILRAGYTVVNVNPLYTARELEHQLKDSGAEAIFVLENFAC 132 Query: 101 SV-RVLVTSEPRHPALADRLSQCPALQ----TVVLVDAAAAAPATPAPPAPLGRLATLDW 155 ++ +V+ + +H +A AL+ +V+ PA P A + + Sbjct: 133 TLQKVVAHTNIKHIVVASMGELLGALKGTLVNLVVRHVKKLVPAWSLPNA-------ITF 185 Query: 156 NAFLQAAPGPGSRRAHRRIDADIAAILYTSGSTGQPKGVVLSHRNLVAGAQSVAGYL--- 212 + L A G + D DIA + YT G+TG KG VL+HRNLVA +L Sbjct: 186 SQALSQARGKTLQPVTLTHD-DIAFLQYTGGTTGVAKGAVLTHRNLVANVLQAEEWLTPA 244 Query: 213 --GNHPQDRLLAVLPLSFDYGLSQLSTAFLSGASVTLLNYLAP--RDI---LHAVASEGI 265 P D+L+ V L Y + L+ + G LN L P RDI + +A + Sbjct: 245 LKAGKPIDQLVFVCALPL-YHIFALTVCNMLGTREGALNLLIPNPRDIPGFVKELAKYKV 303 Query: 266 TGLAGVPPLWQQL---AGLPWPQGCSLRYLTNSGGALPRSVLAQLRRALPQAQVFLMYGL 322 V L+ L A R G A+ +SV + ++ L + YGL Sbjct: 304 NTFPAVNTLYNALLNNADFARLDFSGYRCCVGGGMAVQKSVADKWKK-LTGCPIIEGYGL 362 Query: 323 TEAFRSTYLPPAELDRRPDSIGRAIPNAEIMVVRPDGSPCAPGEVGELVHRGALVAQGYW 382 +E P +D +IG +P+ E ++ DG P G+ GE+ RG V GYW Sbjct: 363 SETSPIASANPCTIDEYTGTIGLPLPSTEFAILDDDGQPVPLGQPGEIAIRGPQVMPGYW 422 Query: 383 NDPARTAERFRPAPGREAALGTPELAVWSGDSVRMDEQGYLYFVGRADDMIKVSGYRISP 442 P TA+ + TP+ +GD MDE+GY V R DMI VSG+ + P Sbjct: 423 QRPDETAK-----------VMTPDGFFKTGDIGVMDERGYTKIVDRKKDMILVSGFNVYP 471 Query: 443 HEVEEVLHATGLADQFAVVGVAHPQQGQAV-VAVAPSSCTASLEAVQQACRAHLPSYMLP 501 +EVE V+ + A VGV G+AV + V T S + C+ Y P Sbjct: 472 NEVEGVVAQHPGVLECACVGVPDEHTGEAVKLFVVRKDKTLSAAQLMDYCKEQFTGYKKP 531 Query: 502 AALHRWPGELPRSPNGKLDRKLMQ 525 + + ELP++ GK+ R+ ++ Sbjct: 532 KYIE-FRDELPKTNVGKILRRELR 554 >gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 132 bits (331), Expect = 9e-32, Method: Compositional matrix adjust. Identities = 144/524 (27%), Positives = 223/524 (42%), Gaps = 69/524 (13%) Query: 35 LAAGVEQVAAALLALGLGPGERVAVYLEKRMETVLALFGAAAAGLVFVPINPLLKPAQVS 94 + A V+ +A AL G+ G+++ V+ + + + A G+V+VP N + P + + Sbjct: 68 INARVDALAHALTRRGVKKGDKMLVHSRNNQQIFESAWAAFKMGVVWVPTNFRITPPEAA 127 Query: 95 HILQDCSVRVLVTSEPRHPALADRLSQCPALQTVV-LVDAAAAAPATPAPPAPLGRLATL 153 ++ Q V++ + + PAL+ VV L DA R L Sbjct: 128 YLGQSSGACVMLYDRGFAQYVDAVKAASPALEHVVALADA---------------RAGEL 172 Query: 154 DWNAFLQAAPGPGSRRAHRRIDADIAAILYTSGSTGQPKGVVLSHRNLVAGAQSVAGYL- 212 D+ A ++ G R A D D YTSG+TG PK +LSH + A V +L Sbjct: 173 DYEALVREGSGAAFREAEVDYD-DPLWFFYTSGTTGHPKAGMLSHGQM---AFVVTNHLA 228 Query: 213 ----GNHPQDRLLAVLPLSFDYGLSQLSTAFLSGASVTLLNY-LAPRDILHAVASEGITG 267 G Q R L V PLS G+ + AS+ L + L + V + Sbjct: 229 DLMPGLTHQSRSLVVAPLSHGAGVHAVINTARGAASILLSSERLVVEEAWQLVEKYRVDN 288 Query: 268 LAGVPPLWQQLA---GLPWPQGCSLRYLTNSGGALPRS----VLAQLRRALPQAQVFLMY 320 L VP + + L + SL+++ +G + R+ L +L + L Q Y Sbjct: 289 LFTVPTIVKILVEDESVDRYDHSSLKHVIYAGAPMYRADQVYALKKLGKVLVQ-----YY 343 Query: 321 GLTEAFRS-TYLPPA---ELDRRPD----SIGRAIPNAEIMVVRPDGSPCAPGEVGELVH 372 GL E + T+LPP E D P + G +I ++ DG AP E GE+ Sbjct: 344 GLGEVTGNITFLPPYLHFEDDAHPQARVGTCGMPRTGMQIAILNDDGQELAPFETGEICV 403 Query: 373 RGALVAQGYWNDPARTAERFRPAPGREAALGTPELAVW--SGDSVRMDEQGYLYFVGRAD 430 RG V GY N+P A+ F+ W +GD +D+ G+LY GR+ Sbjct: 404 RGPAVFMGYHNNPEANAKAFKHD--------------WFHTGDLGHVDQDGFLYITGRSS 449 Query: 431 DMIKVSGYRISPHEVEEVLHATGLADQFAVVGVAHPQQGQAVVAVAPSSCTASLEAVQQA 490 DM G + P E+EE L + AV+GV P+ G++ +AV C A + +A Sbjct: 450 DMYISGGSNVYPREIEEALLTHPAVSEVAVLGVPDPKWGESGLAVI--VCKAGQQVDGEA 507 Query: 491 CRAHLPS----YMLPAALHRWPGELPRSPNGKLDRKLMQQTLAD 530 AHL S Y P W +P+S GK+ +K ++ L + Sbjct: 508 LLAHLESRIGKYKWPRRFIYWD-SMPKSGYGKIVKKQIKALLEE 550 >gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 549 Score = 119 bits (299), Expect = 4e-28, Method: Compositional matrix adjust. Identities = 144/510 (28%), Positives = 219/510 (42%), Gaps = 59/510 (11%) Query: 40 EQVAAALLALGLGPGERVAVYLEKRMETVLALFGAAAAGLVFVPINPLLKPAQVSHILQD 99 ++ A AL A G+G G+R+ +YL +R+ETV+ A G + VP+ L P +++ L + Sbjct: 73 DRFANALRAAGVGRGDRIGIYLSQRIETVITHLAAYKLGAITVPLFYLFGPDAIAYRLDN 132 Query: 100 CSVRVLVT--SEPRHPALADRLSQCPALQTVVLVDAAAAAPATPAPPAPLGRLATLDWNA 157 LVT S LA L PAL+ V V+++ L T D+ Sbjct: 133 SGAVALVTDASGMEKATLAGEL---PALRLVFCVESS-----------DLVLPQTTDFWD 178 Query: 158 FLQAAPGPGSRRAHRRIDADIAAILYTSGSTGQPKGVVLSHRNLVAGAQSVAGYLGNHPQ 217 L AAP + D A I+YTSG+TG+ KG + +HR L+ V + PQ Sbjct: 179 RLHAAPA--ELDPVLTLADDPAMIIYTSGTTGKAKGALHAHRVLLGHLPGVEVSHDSFPQ 236 Query: 218 --DRLLAVLPLSFDYGL-SQLSTAFLSGASVTL--LNYLAPRDILHAVASEGITGLAGVP 272 DR ++ GL L + G +V L ++ +A I + P Sbjct: 237 EGDRFWTPADWAWIGGLLDVLLPSLYHGVAVVARRLEKFDAAEVFGLLARHQIRNVFFPP 296 Query: 273 PLWQQLAGLPWPQG---CSLRYLTNSGGALPRSVLAQLRRALPQAQVFLMYGLTEAF--- 326 + L G + SLR + + G L ++A R AL + YG TE Sbjct: 297 TALKMLRGAATVRAQADFSLRSVASGGETLGDDLIAWGREAL-GVTINEFYGQTECNLVV 355 Query: 327 -RSTYLPPAELDRRPDSIGRAIPNAEIMVVRPDGSPCAPGEVGELVHRGA--LVAQGYWN 383 S++ P+ S+GRA+P + +V G G VG + R ++ YW Sbjct: 356 SSSSHCYPSV----SGSMGRAVPGHVVQIVDEQGQVLPHGTVGNIAIRAPDPVMFLRYWR 411 Query: 384 DPARTAERFRPAPGREAALGTPELAVWSGDSVRMDEQGYLYFVGRADDMIKVSGYRISPH 443 + T E+F + +GD MDEQGY+ ++GR DD+I +GYRI P Sbjct: 412 NEEATREKFAGD------------FLLTGDLGSMDEQGYIRYLGRNDDVITSAGYRIGPA 459 Query: 444 EVEEVLHATGLADQFAVVGVAHPQQGQAVVA-------VAPSSCTASLEAVQQACRAHLP 496 +EE L AVVGV + + V A V P + +QQ RA L Sbjct: 460 AIEECLMRHPAVRIAAVVGVKDALRTEVVKAFVVLKDGVTPDEALKA--ELQQHVRAQLA 517 Query: 497 SYMLPAALHRWPGELPRSPNGKLDRKLMQQ 526 ++ P L + LP + GK+ RK +++ Sbjct: 518 AHEYP-RLISFVAALPTTATGKIMRKTLKE 546 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,615,997 Number of extensions: 67544 Number of successful extensions: 323 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 274 Number of HSP's successfully gapped: 19 Length of query: 539 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 449 Effective length of database: 1,193,720 Effective search space: 535980280 Effective search space used: 535980280 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)