BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0010.p (548 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312035|ref|YP_003776127.1| methyl-accepting chemotaxis tra... 1084 0.0 gi|300313312|ref|YP_003777404.1| methyl-accepting chemotaxis pro... 382 e-107 gi|300312219|ref|YP_003776311.1| methyl-accepting chemotaxis tra... 340 1e-94 gi|300312038|ref|YP_003776130.1| methyl-accepting chemotaxis tra... 335 8e-93 gi|300310474|ref|YP_003774566.1| methyl-accepting chemotaxis tra... 334 1e-92 >gi|300312035|ref|YP_003776127.1| methyl-accepting chemotaxis transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 548 Score = 1084 bits (2803), Expect = 0.0, Method: Compositional matrix adjust. Identities = 547/548 (99%), Positives = 547/548 (99%) Query: 1 MTITKRLIITLSTALLALLFVGADGLWQLQRAQQRLELIQNRIIPGMDSLNLLKGYLADS 60 MTITKRLIITLSTALLALLFVGADGLWQLQRAQQRLELIQNRIIPGMDSLNLLKGYLADS Sbjct: 1 MTITKRLIITLSTALLALLFVGADGLWQLQRAQQRLELIQNRIIPGMDSLNLLKGYLADS 60 Query: 61 RLAGYRLSVFANLSDKSGLQKAVDTANKLLDEEFAHYERDLIFDEKDRALIQADKANIEA 120 RLAGYRLSVFANLSDKSGLQKAVDTANKLLDEEFAHYERDLIFDEKDRALIQADKANIEA Sbjct: 61 RLAGYRLSVFANLSDKSGLQKAVDTANKLLDEEFAHYERDLIFDEKDRALIQADKANIEA 120 Query: 121 YRKALVPFFAAAYANDMDGVRATLQAGTPLAISAAAAKKGFEDHILIGRKMIDTIKQESD 180 YRKALVPFFAAAYANDMDGVRATLQAGTPLAISAAAAKKGFEDHILIGRKMIDTIKQESD Sbjct: 121 YRKALVPFFAAAYANDMDGVRATLQAGTPLAISAAAAKKGFEDHILIGRKMIDTIKQESD 180 Query: 181 EAYAFALRTMIAVVVVAVLLTGLLGLQVVRSVIRSLGKIQHTFEHVSQTLDLSRPVPVER 240 EAYAFALRTMIAVVVVAVLLTGLLGLQVVRSVIRSLGKIQHTFEHVSQTLDLSRPVPVER Sbjct: 181 EAYAFALRTMIAVVVVAVLLTGLLGLQVVRSVIRSLGKIQHTFEHVSQTLDLSRPVPVER 240 Query: 241 MDEIGRTAAAFNKLLARIVDVVATTRTSTDSVTVAARQIAAGNADLSARTEQQAAALEES 300 MDEIGRTAAAFNKLLARIVDVVATTRTSTDSVTVAARQIAAGNADLSARTEQQAAALEES Sbjct: 241 MDEIGRTAAAFNKLLARIVDVVATTRTSTDSVTVAARQIAAGNADLSARTEQQAAALEES 300 Query: 301 ASSLEQLTSVVLQNTQNARQANELAQSASHIASQGGDVVRQVVDTMNSINESSRKIVDII 360 ASSLEQLTSVVLQNTQNARQANELAQSASHIASQGGDVVRQVVDTMNSINESSRKIVDII Sbjct: 301 ASSLEQLTSVVLQNTQNARQANELAQSASHIASQGGDVVRQVVDTMNSINESSRKIVDII 360 Query: 361 AVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAAAAKEIKELIDDSV 420 AVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAAAAKEIKELIDDSV Sbjct: 361 AVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAAAAKEIKELIDDSV 420 Query: 421 SKVGSGSKLVEQAGTTMSDIVDSVQRVTVIVGDIANASEEQSAGISQVNQAIAQMDQGTQ 480 SKVGSGSKLVEQAGTTMSDIVDSVQRVTVIVGDIANASEEQSAGISQVNQAIAQMDQGTQ Sbjct: 421 SKVGSGSKLVEQAGTTMSDIVDSVQRVTVIVGDIANASEEQSAGISQVNQAIAQMDQGTQ 480 Query: 481 QNAALVEEAAAAAHALQQQAETLESVVAVFKLEGRLNGSIPVIQAPASAARLPASPAARQ 540 QNAALVEEAAAAAHALQQQAETLESVVAVFKLEGRLNGSIPVIQAPASAARLPASPAARQ Sbjct: 481 QNAALVEEAAAAAHALQQQAETLESVVAVFKLEGRLNGSIPVIQAPASAARLPASPAARQ 540 Query: 541 AEPVLEEF 548 AEP LEEF Sbjct: 541 AEPALEEF 548 >gi|300313312|ref|YP_003777404.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 544 Score = 382 bits (982), Expect = e-107, Method: Compositional matrix adjust. Identities = 226/512 (44%), Positives = 318/512 (62%), Gaps = 4/512 (0%) Query: 1 MTITKRLIITLSTALLALLFVGADGLWQLQRAQQRLELIQNRIIPGMDSLNLLKGYLADS 60 ++I RL I + ++ + + + ++ R ++P + SL+ + + Sbjct: 3 LSIVNRLYILIGAVIIGCVVLAGANMVKMNRTYADASFASENVVPSLLSLSTITQGINGM 62 Query: 61 RLAGYRLSVFANLSDKSGLQKAVDTANKLLDEEFAHYERDLIFDEKDRALIQADKANIEA 120 R+A +R D+ ++ + ++A K +++ YE++ + D D+ L++A + + + Sbjct: 63 RIATWRYLALTESVDRGAIESSFNSAKKRVEDALQKYEQEQLADATDKELLRAVRVSYQE 122 Query: 121 YRKALVPFFAAAYANDMDGVRATLQAGTPLAISAAAAKKGFEDHILIGRKMIDTIKQESD 180 + K+L A A + RA L P+ K + K+ + Sbjct: 123 FDKSLAEALKLANAGQWNEARALLANNQPVIDRL---NKAMTELRAYNEKISQAGAHRAQ 179 Query: 181 EAYAFALRTMIAVVVVAVLLTGLLGLQVVRSVIRSLGKIQHTFEHVSQTLDLSRPVPVER 240 E A A I + + + L+GL + R + RSL + E +++ DL+R + Sbjct: 180 ETMAAARLHAILISAAVIAVVTLMGLLIARKIARSLAEAVELAETIAEG-DLTREIDTSS 238 Query: 241 MDEIGRTAAAFNKLLARIVDVVATTRTSTDSVTVAARQIAAGNADLSARTEQQAAALEES 300 DEIG AA K+ +V +VAT RT ++V A+ QIA GN DLSARTEQQA++LE++ Sbjct: 239 TDEIGHLMAAMAKMNRNLVTIVATVRTGVNTVAAASSQIATGNMDLSARTEQQASSLEQT 298 Query: 301 ASSLEQLTSVVLQNTQNARQANELAQSASHIASQGGDVVRQVVDTMNSINESSRKIVDII 360 A++ E+LTS V QN +NA QA++LA +AS IA++GG+VV+QVV TM I SS I DII Sbjct: 299 AAATEELTSTVKQNAENALQADQLASAASGIAAEGGEVVKQVVTTMEEIRSSSLNIADII 358 Query: 361 AVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAAAAKEIKELIDDSV 420 +VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVA+EVRSLAQRSA+AAKEIK+LIDDSV Sbjct: 359 SVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSASAAKEIKQLIDDSV 418 Query: 421 SKVGSGSKLVEQAGTTMSDIVDSVQRVTVIVGDIANASEEQSAGISQVNQAIAQMDQGTQ 480 SKV +GS+LV AG TM +V+SV+RVT +V +I+ AS EQS GI QVNQAI+ MDQ TQ Sbjct: 419 SKVENGSQLVMSAGNTMQQVVESVRRVTDVVTEISAASREQSLGIEQVNQAISHMDQSTQ 478 Query: 481 QNAALVEEAAAAAHALQQQAETLESVVAVFKL 512 +NAALVEEA AAA +LQ+QA LE V VFKL Sbjct: 479 ENAALVEEATAAAQSLQEQARQLERAVNVFKL 510 >gi|300312219|ref|YP_003776311.1| methyl-accepting chemotaxis transducer transmembrane I protein [Herbaspirillum seropedicae SmR1] Length = 563 Score = 340 bits (873), Expect = 1e-94, Method: Compositional matrix adjust. Identities = 232/553 (41%), Positives = 325/553 (58%), Gaps = 34/553 (6%) Query: 1 MTITKRLIITLSTALLALLFVGADGLWQLQRAQQRLELIQNRIIPGMDSLNLLKGYLADS 60 MTI ++L + + +L ++ GL+Q R IP + +LN A Sbjct: 31 MTIARKLYLLIVAVVLGVIMQAVVGLYQSHRIASSAGYSATDTIPSVLALNG-----ATD 85 Query: 61 RLAGYRLSVFANLSDKSGLQKAVDTANKLLDEE---FAHYERDLIFDEKDRALIQADKAN 117 + G RL++ + + + +K V L + F +ER I D+ DR +++AD+ Sbjct: 86 AIYGIRLNIARYVIEPTAREKTVANMAALHQKAQDFFDQFERCCISDDTDRRMLEADRRF 145 Query: 118 IEAYRKALVPFFAAAYANDMDGVRATLQAGTPLAISAAAAKKGFEDHILIGRKMIDTIKQ 177 Y K + A D +R ++ +A + +H ++ + Sbjct: 146 FAEYEKHRMAMVDLGSAGKEDALRVAME--EKMAPTGQRLMAVLNEHKNYNEQLGRQGAE 203 Query: 178 ESDEAYAFALRTMIAVVVVAVLLTGLLGLQVVRSVIRSLGKIQHTFEHVSQTL---DLSR 234 +DE ++ IA + +++ L L +VR++ SL H ++ T+ DLS Sbjct: 204 AADEHLQLSIYLTIAAATLVIVMVSALSLLLVRNIRHSL----HAATELADTIGRGDLSS 259 Query: 235 PVPVERMDEIGRTAAAFNKLLARIVDVVATTRTSTDSVTVAARQIAAGNADLSARTEQQA 294 + + DE+ + + ++ +V ++ S ++ A R+IA+GN DLS+RTE QA Sbjct: 260 QIVLRGRDEVTQLMLSLQRMTGSLVQILGKVNDSAATIETATREIASGNLDLSSRTEAQA 319 Query: 295 AALEESASSLEQLTSVVLQNTQNARQANELAQSASHIASQGGDVVRQVVDTMNSINESSR 354 +LEE+ S++ QLT+ V QN NARQA+ELAQSAS +A Q G VV +V+ TM +IN SS Sbjct: 320 GSLEETVSAMGQLTTTVKQNADNARQADELAQSASGVAGQAGKVVDEVILTMEAINASSL 379 Query: 355 KIVDIIAVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAAAAKEIKE 414 KIVDII+VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSA+AA+EIKE Sbjct: 380 KIVDIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAREIKE 439 Query: 415 LIDDSVSKVGSGSKLVEQAGTTMSDIVDSVQRVTVIVGDIANASEEQSAGISQVNQAIAQ 474 LIDDSV+KV SGS+LV QAG+TM ++V SV+RV+ IV DI+ AS EQS GI+QVNQAI Sbjct: 440 LIDDSVNKVQSGSRLVLQAGSTMEEVVTSVRRVSGIVRDISVASGEQSDGIAQVNQAIGM 499 Query: 475 MDQGTQQNAALVEEAAAAAHALQQQAETLESVVAVFKLEGRLNGSIPVIQAPASAARLPA 534 MDQ TQQNAALVEEAAAA+ +L++QA+ L V+VF+LE R Sbjct: 500 MDQTTQQNAALVEEAAAASQSLREQAKGLAQAVSVFRLE-----------------RSAE 542 Query: 535 SPAARQAEPVLEE 547 +P EPV E+ Sbjct: 543 APQPESTEPVQEQ 555 >gi|300312038|ref|YP_003776130.1| methyl-accepting chemotaxis transmembrane I protein [Herbaspirillum seropedicae SmR1] Length = 579 Score = 335 bits (858), Expect = 8e-93, Method: Compositional matrix adjust. Identities = 215/375 (57%), Positives = 266/375 (70%), Gaps = 3/375 (0%) Query: 167 IGRKMIDTIKQESDEAYAFALRTMIAVVVVAVLLTGLLGLQVVRSVIRSLGKIQHTFEHV 226 + K D +++D YA A +I V + L LL + V R + R L + + V Sbjct: 170 VNDKGADISNKQADAIYAQARLWIIVTVAACIGLAMLLAVWVSRLIARPLEEAVSVAQRV 229 Query: 227 SQTLDLSRPVPVERMDEIGRTAAAFNKLLARIVDVVATTRTSTDSVTVAARQIAAGNADL 286 + DL+ + DE G+ A + +V +V R T ++T A+ +IA+GN DL Sbjct: 230 AGG-DLTTRIEPAGKDETGQLLVALKSMNDSLVGIVGQVRMGTQTITTASTEIASGNLDL 288 Query: 287 SARTEQQAAALEESASSLEQLTSVVLQNTQNARQANELAQSASHIASQGGDVVRQVVDTM 346 S+RTE+QA++LEE+AS++E+LTS V QN NARQAN+LA SAS +ASQGG VV QVVDTM Sbjct: 289 SSRTEEQASSLEETASAMEELTSTVKQNADNARQANQLAVSASEVASQGGAVVNQVVDTM 348 Query: 347 NSINESSRKIVDIIAVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSA 406 SIN SSRKI DII VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSA Sbjct: 349 GSINASSRKIADIIGVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSA 408 Query: 407 AAAKEIKELIDDSVSKVGSGSKLVEQAGTTMSDIVDSVQRVTVIVGDIANASEEQSAGIS 466 AAKEIKELI+ SV++V GSKLVE+AG TMS++V SV+RVT IVG+I++AS+EQS GI Sbjct: 409 GAAKEIKELIEGSVAQVDMGSKLVERAGITMSEVVQSVRRVTDIVGEISSASQEQSEGIE 468 Query: 467 QVNQAIAQMDQGTQQNAALVEEAAAAAHALQQQAETLESVVAVFKLEGRLNGSIPVIQAP 526 QVN AIAQMD+ TQQNAALVEEAAAAA +LQ+QAETL V FKLE + + P + P Sbjct: 469 QVNLAIAQMDEVTQQNAALVEEAAAAAQSLQEQAETLTQTVGFFKLEQQPDE--PASRPP 526 Query: 527 ASAARLPASPAARQA 541 AA + + A R A Sbjct: 527 VRAASVARTAAVRHA 541 >gi|300310474|ref|YP_003774566.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 334 bits (856), Expect = 1e-92, Method: Compositional matrix adjust. Identities = 233/515 (45%), Positives = 315/515 (61%), Gaps = 8/515 (1%) Query: 1 MTITKRLIITLSTALLALLFVGADGLWQLQRAQQRLELIQNRIIPGMDSLNLLKGYLADS 60 + I K+L ++ + + L G + +L++ + I ++ +P MD+ L+G L Sbjct: 7 LNIAKKLALSFAVVIALTLLQGLFAIKELKQVNKASGEIVSKWMPTMDAARELQGSLPPL 66 Query: 61 RLAGYRLSVFANLSDKSGLQKAVDTANKLLDEEFAHYERDLIFDEKDRALIQADKANIEA 120 RL L +++ KA+ L + A +E+ + ++ Q K N+ A Sbjct: 67 RLNELELVTAVTPQERAAANKAIKDRLDALTRQRAIFEQRMSEPQEKEQYAQFLK-NLTA 125 Query: 121 YRKALVPFFAAAYANDMDGVRATLQAGTPLAISAAAAKKGFEDHIL-IGRKMIDTIKQES 179 Y K A D RA + + G D I+ I +E+ Sbjct: 126 YVKVDEQLAEMAANQQYDETRALFNGDSAKLYRSMV---GNLDAIVKINTSGSAQADEEA 182 Query: 180 DEAYAFALRTMIAVVVVAVLLTGLLGLQVVRSVIRSLGKIQHTFEHVSQTLDLS-RPVPV 238 + A R ++ ++ VL+ LL V +V R L + + V++ DL+ R P Sbjct: 183 STVFRGAQRWIVGLLAATVLIASLLAFLVATNVSRPLKEAVQIAQRVARG-DLTLRIRPA 241 Query: 239 ERMDEIGRTAAAFNKLLARIVDVVATTRTSTDSVTVAARQIAAGNADLSARTEQQAAALE 298 R DE G A + + D+V+ R TD++T A+ +IA GN DLS RTEQQA +LE Sbjct: 242 GR-DETGALMEALRAMNESLRDIVSEVRQGTDTITTASHEIARGNLDLSNRTEQQAGSLE 300 Query: 299 ESASSLEQLTSVVLQNTQNARQANELAQSASHIASQGGDVVRQVVDTMNSINESSRKIVD 358 E+AS++E+LTS V QN NARQAN+LA +AS IASQGG+VV QVV TM I ESSR+I D Sbjct: 301 ETASAMEELTSTVTQNADNARQANQLAATASDIASQGGEVVGQVVQTMEGITESSRRIAD 360 Query: 359 IIAVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAAAAKEIKELIDD 418 II+VIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSA AAKEIKELIDD Sbjct: 361 IISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSAGAAKEIKELIDD 420 Query: 419 SVSKVGSGSKLVEQAGTTMSDIVDSVQRVTVIVGDIANASEEQSAGISQVNQAIAQMDQG 478 SV KV +GS LVE+AG+TM+++V SV+RVT +V +I+ A++EQS GI++VN AI QMD+ Sbjct: 421 SVQKVSAGSALVERAGSTMTEVVASVRRVTDVVAEISAATQEQSGGINEVNLAITQMDET 480 Query: 479 TQQNAALVEEAAAAAHALQQQAETLESVVAVFKLE 513 TQQNAALVE+AAAAA +L+ QA L +VV FKL+ Sbjct: 481 TQQNAALVEQAAAAADSLKGQASNLAAVVGKFKLD 515 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.128 0.330 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,042,838 Number of extensions: 32941 Number of successful extensions: 391 Number of sequences better than 1.0e-08: 71 Number of HSP's gapped: 243 Number of HSP's successfully gapped: 73 Length of query: 548 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 458 Effective length of database: 1,193,720 Effective search space: 546723760 Effective search space used: 546723760 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)