BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0035.0003.p (349 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbasp... 714 0.0 gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Her... 138 7e-34 gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbasp... 124 1e-29 gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Her... 110 2e-25 gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Her... 93 4e-20 >gi|300312028|ref|YP_003776120.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 349 Score = 714 bits (1842), Expect = 0.0, Method: Compositional matrix adjust. Identities = 347/349 (99%), Positives = 347/349 (99%) Query: 1 MQYRPLGRTDLKVSLITLGTMTWGEQNSEADAHAQLDYAVERGINLIDVAEMYPVPPKAE 60 MQYRPLGRTDLKVSLITLGTMTWGEQNSEADAHAQLDYAVERGINLIDVAEMYPVPPKAE Sbjct: 1 MQYRPLGRTDLKVSLITLGTMTWGEQNSEADAHAQLDYAVERGINLIDVAEMYPVPPKAE 60 Query: 61 TQGLTERYLGTWLKKSGKRDQLLIATKCTGPARKPHNPRHVRGGINNLDRKGLTEALHGS 120 TQGLTERYLGTWLKKSGKRDQLLIATKCTGPARKPHNPRHVRGGINNLDRKGLTEALHGS Sbjct: 61 TQGLTERYLGTWLKKSGKRDQLLIATKCTGPARKPHNPRHVRGGINNLDRKGLTEALHGS 120 Query: 121 LERLQLDHVDLYQLHWPDRSVNSFGQLGYQHVEDESTVPIEETLTVLSEFVKAGKIRAIG 180 LERLQLDHVDLYQLHWPDRSVNSFGQLGYQHVEDESTVPIEETLTVLSEFVKAGKIR IG Sbjct: 121 LERLQLDHVDLYQLHWPDRSVNSFGQLGYQHVEDESTVPIEETLTVLSEFVKAGKIRTIG 180 Query: 181 LSNETAWGVAQFLRAAEQHGLERIVTIQNPYNLLNRSFEINLAEFAHREQVGLLAYSPLA 240 LSNETAWGVAQFLRAAEQHGLERIVTIQNPYNLLNRSFEINLAEFAHREQVGLLAYSPLA Sbjct: 181 LSNETAWGVAQFLRAAEQHGLERIVTIQNPYNLLNRSFEINLAEFAHREQVGLLAYSPLA 240 Query: 241 FGMLSGKYLDGARPAGGRLTLFERFSRYTNAQAERATAEYVALARQHGLDPAQMALAYVN 300 FGMLSGKYLDGARPAGGRLTLFERFSRYTNAQAERATAEYVALARQHGLDPAQMALAYVN Sbjct: 241 FGMLSGKYLDGARPAGGRLTLFERFSRYTNAQAERATAEYVALARQHGLDPAQMALAYVN 300 Query: 301 SRPFLTSNIIGATTLAQLRSNIDSAELQLDEDVLAAIEAIHRRQPNPAP 349 SRPFLTSNIIGATTLAQLRSNIDSAELQL EDVLAAIEAIHRRQPNPAP Sbjct: 301 SRPFLTSNIIGATTLAQLRSNIDSAELQLGEDVLAAIEAIHRRQPNPAP 349 >gi|300309662|ref|YP_003773754.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 335 Score = 138 bits (348), Expect = 7e-34, Method: Compositional matrix adjust. Identities = 100/348 (28%), Positives = 173/348 (49%), Gaps = 46/348 (13%) Query: 1 MQYRPLGRTDLKVSLITLGTMTWGEQNSEADAHAQLDYAVERGINLIDVAEMYPVPPKAE 60 M YR LG+++LKVS + LG+M +G+Q ++ + A E G+N ID A++Y Sbjct: 1 MHYRRLGKSNLKVSALCLGSMMFGDQTDLQESGRIVASAREHGVNFIDTADVY------- 53 Query: 61 TQGLTERYLGTWLKKSGKRDQLLIATKC-TGPARKPHNPRHVRGGINNLDRKGLTEALHG 119 T+G +E +G L +G+R + ++A+K + KP+ +N R + Sbjct: 54 TKGKSEEIVGKLL--AGQRHEWVLASKLGNAMSGKPNE--------SNYSRSWIVRETEN 103 Query: 120 SLERLQLDHVDLYQLHWPDRSVNSFGQLGYQHVEDESTVPIEETLTVLSEFVKAGKIRAI 179 L RL D++D+ +H + +DE+ +EE + L + ++ GKIR Sbjct: 104 ILTRLDTDYLDILYMH--------------RDYQDEN---LEEAVQALGDLIRVGKIRGF 146 Query: 180 GLSNETAWGVAQFLRAAEQHGLERIVTIQNPYNLLNRSFEINLAEFAHREQVGLLAYSPL 239 G+SN W +A+ +R E++ + + Q YNLLNR E+ + +G++ YSP+ Sbjct: 147 GVSNFRGWRIAEIVRLCEKNNVPLPLVCQPYYNLLNRQPEVEILPACAHYGLGVVPYSPI 206 Query: 240 AFGMLSGKYLDGARPA-------GGRLTLFERFSRYTNAQAERATAEYVALARQHGLDPA 292 A G+L+GKY G +PA G + + F + A+R A G+ Sbjct: 207 ARGVLTGKYQPGQKPAQDTRAGRGDKRMMETEFREESLLIAQRLQQHCEA----RGVKLG 262 Query: 293 QMALAYVNSRPFLTSNIIGATTLAQLRSNIDSAELQLDEDVLAAIEAI 340 Q A A+V + ++S I G TLAQ+ D+AEL++ + A ++++ Sbjct: 263 QFATAWVLANSAISSVIAGPRTLAQMEDYYDAAELKISAEEEALVDSL 310 >gi|300312649|ref|YP_003776741.1| oxidoreductase protein [Herbaspirillum seropedicae SmR1] Length = 343 Score = 124 bits (310), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 119/352 (33%), Positives = 167/352 (47%), Gaps = 56/352 (15%) Query: 4 RPLGRTDLKVSLITLGTMTWGE---QNSEADAHAQLDYAVERGINLIDVAEMYPVPPKAE 60 R LG VS I LG M + Q +A++ A +D A+E GINL+D A+MY Sbjct: 18 RRLGSNGPLVSAIGLGCMGMSDFYAQRDDAESLATIDRALELGINLLDTADMY------- 70 Query: 61 TQGLTERYLGTWLKKSGKRDQLLIATKCTGPARKPHNPRHVRGGINNLDRKG--LTEALH 118 E LG +K G+RD+ IATK G R P +P G +D + +A+ Sbjct: 71 GPYTNEELLGRAIK--GRRDKFFIATKF-GIRRDPSDP-----GARGVDGSPAYIRQAVE 122 Query: 119 GSLERLQLDHVDLYQLHWPDRSVNSFGQLGYQHVEDESTVPIEETLTVLSEFVKAGKIRA 178 GSL+RL ++ +DLY YQH D +T PIE T+ VL++ V+AGKIR Sbjct: 123 GSLKRLGVETIDLY----------------YQHRIDPAT-PIEVTMGVLADLVQAGKIRY 165 Query: 179 IGLSNETAWGVAQFLRAAEQHGLERIVTIQNPYNLLNRSFEINLAEFAHREQVGLLAYSP 238 IGLS +A A RA H + + +Q+ Y+L R E + +G +AYSP Sbjct: 166 IGLSEASA---ATLERA---HRVHPVTALQSEYSLWTRDPEAEVLAACRTLGIGFVAYSP 219 Query: 239 LAFGMLSGKYL-------DGARPAGGRLTLFERFSRYTNAQAERATAEYVALARQHGLDP 291 L G L+G D R + R E F+R N Q E +A QHG P Sbjct: 220 LGRGFLTGAIQRFEDLAEDDFRRSNPRFQ-GENFAR--NLQLAEKVKE---MAGQHGCTP 273 Query: 292 AQMALAYVNSRPFLTSNIIGATTLAQLRSNIDSAELQLDEDVLAAIEAIHRR 343 +Q+ALA+V ++ I G L N S ++L + L A++AI R Sbjct: 274 SQLALAWVMAQDPHIVPIPGTKRRRYLEDNAGSVGVKLAPEDLQALDAIFPR 325 >gi|300313828|ref|YP_003777920.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 344 Score = 110 bits (275), Expect = 2e-25, Method: Compositional matrix adjust. Identities = 106/351 (30%), Positives = 163/351 (46%), Gaps = 51/351 (14%) Query: 1 MQYRPLGRTDLKVSLITLGT-MTWGEQNSEADAHAQLDYAVERGINLIDVAEMYPVPPKA 59 M YR GR+ L + I+LG +G ++ A L A +RG+ D+A Y P Sbjct: 14 MPYRRTGRSGLLLPAISLGLWQNFGGVDNFETGRAVLRRAFDRGVTHFDLANNYGPP--- 70 Query: 60 ETQGLTERYLGTWLKKS--GKRDQLLIATKC-----TGPARKPHNPRHVRGGINNLDRKG 112 G E G W+ K RD+L+I++K GP P R K Sbjct: 71 --AGSAEENFGRWMAKDFLPYRDELVISSKAGWQMWPGPYGGPSGAR-----------KH 117 Query: 113 LTEALHGSLERLQLDHVDLYQLHWPDRSVNSFGQLGYQHVEDESTVPIEETLTVLSEFVK 172 L + SL+R+ LD+VD++ Y H D T P+EET+ L+ + Sbjct: 118 LIASCEQSLKRMGLDYVDIF----------------YSHRVDADT-PLEETMGALAHLHR 160 Query: 173 AGKIRAIGLSNETAWGVAQFLRAAEQHGLERIVTIQNPYNLLNRSFEINLAEFAHREQVG 232 GK +G+S+ + + + G+ ++ N Y++LNR+ E L VG Sbjct: 161 QGKALYVGISSYSPELTRKAAAILKSEGVPLLIHQPN-YSMLNRAIEGGLLPALEELGVG 219 Query: 233 LLAYSPLAFGMLSGKYLDGARPAGGRLT----LFERFSRYTNAQAERATAEYVALARQHG 288 + +SPLA G+L+ KYL G P G R+T L +R N RA E +A + G Sbjct: 220 CIGFSPLAQGLLTSKYLGGV-PEGARVTRAPSLPQRMLSEDNLARIRALNE---IASKRG 275 Query: 289 LDPAQMALAYVNSRPFLTSNIIGATTLAQLRSNIDSA-ELQLDEDVLAAIE 338 AQMA+A+V +TS +IGA T+ QL ++D+ +L ++ LA+I+ Sbjct: 276 QTLAQMAIAWVLRDARVTSALIGARTVEQLDDSLDAVNKLDFTQEELASID 326 >gi|300312019|ref|YP_003776111.1| aryl-alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 329 Score = 92.8 bits (229), Expect = 4e-20, Method: Compositional matrix adjust. Identities = 109/362 (30%), Positives = 167/362 (46%), Gaps = 54/362 (14%) Query: 1 MQYRPLGRTDLKVSLITLGTM----TWGEQNSEADAHAQLDYAVERGINLIDVAEMYPVP 56 M+ R GRT +VS I G +WG+ +++ DA A L A++ G++ ID A++Y Sbjct: 1 MKSRRFGRTGAQVSEIGFGAWAIGGSWGDVSAQ-DAKAALHAALDAGVSFIDTADVY--- 56 Query: 57 PKAETQGLTERYLGTWLKKSGKRDQLLIATKCTGPARKPHNPRHVRGGINNLDRKGLTEA 116 G +E+ + LK+ G ++ +ATK A + +P HV G N DR L+ Sbjct: 57 ----GDGRSEQLIAQVLKERGG-ERPFVATK----AGRRLSP-HVASGYN--DRAQLSAF 104 Query: 117 LHGSLERLQLDHVDLYQLHWPDRSVNSFGQLGYQHVEDESTVPIEETLTVLSEFVKAGKI 176 + SL+ L +D +DL QLH P V YQ +E L V AGKI Sbjct: 105 IDRSLKNLGVDCLDLVQLHCPPTEVY------YQ----------QEVFGHLDALVAAGKI 148 Query: 177 RAIGLSNETAWGVAQFLRAAEQHGLERIVTIQNPYNLLNRSFEINLAEFAHREQVGLLAY 236 R G+S E V + L+A E ++ + I N + R + L E A R + ++A Sbjct: 149 RHYGVSVEK---VEEALKAIEYPNVKSVQIIFNIFR--QRPISLFLQE-AQRRDIAVIAR 202 Query: 237 SPLAFGMLSGKYLDGARPA----------GGRLTLFERFSRYTNAQAERATAEYVALARQ 286 PLA G+L+GK A G + E FS A A + AL Q Sbjct: 203 VPLASGLLTGKMSAQTTFAADDHRQFNRHGEAFDVGETFSGVPYEVALAAVEKIRALVPQ 262 Query: 287 HGLDPAQMALAYVNSRPFLTSNIIGATTLAQLRSNIDSAEL-QLDEDVLAAIEAIHRRQP 345 G+ AQ AL ++ +++ I GA +AQ +SN ++ L + +V+ A+ AI+ Sbjct: 263 -GVPMAQFALRWILMESGISTVIPGARNVAQAQSNSAASALPPITAEVMQALRAIYLHDI 321 Query: 346 NP 347 P Sbjct: 322 AP 323 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.134 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,092,969 Number of extensions: 44711 Number of successful extensions: 166 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 152 Number of HSP's successfully gapped: 6 Length of query: 349 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 263 Effective length of database: 1,212,660 Effective search space: 318929580 Effective search space used: 318929580 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)