BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0045.p (250 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309410|ref|YP_003773502.1| glutamine ABC transporter perme... 504 e-144 gi|300313611|ref|YP_003777703.1| amino acid ABC transporter perm... 301 5e-83 gi|300311471|ref|YP_003775563.1| amino acid ABC transporter perm... 131 5e-32 gi|300310495|ref|YP_003774587.1| amino acid ABC transporter perm... 119 2e-28 gi|300311602|ref|YP_003775694.1| glutamine ABC transporter perme... 117 1e-27 >gi|300309410|ref|YP_003773502.1| glutamine ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 250 Score = 504 bits (1297), Expect = e-144, Method: Compositional matrix adjust. Identities = 250/250 (100%), Positives = 250/250 (100%) Query: 1 MNSFFQWEIIGEYLPLFVEGTWMTLKAAIICVIAGTCWGLVLGVGRLAEARHGFWKYFLC 60 MNSFFQWEIIGEYLPLFVEGTWMTLKAAIICVIAGTCWGLVLGVGRLAEARHGFWKYFLC Sbjct: 1 MNSFFQWEIIGEYLPLFVEGTWMTLKAAIICVIAGTCWGLVLGVGRLAEARHGFWKYFLC 60 Query: 61 YCVQWPIRFYVSFLRGTPLFVQILLIHFALMPMLINPSGGLILSGDLAREIRSHYGAFAS 120 YCVQWPIRFYVSFLRGTPLFVQILLIHFALMPMLINPSGGLILSGDLAREIRSHYGAFAS Sbjct: 61 YCVQWPIRFYVSFLRGTPLFVQILLIHFALMPMLINPSGGLILSGDLAREIRSHYGAFAS 120 Query: 121 AVLAITLNSGAYVSEIFRAGIQSIDRGQSEASRSLGMSYLATLRRVVLPQAFRRMLPPLG 180 AVLAITLNSGAYVSEIFRAGIQSIDRGQSEASRSLGMSYLATLRRVVLPQAFRRMLPPLG Sbjct: 121 AVLAITLNSGAYVSEIFRAGIQSIDRGQSEASRSLGMSYLATLRRVVLPQAFRRMLPPLG 180 Query: 181 NNAIAIVKDSSLASAIGLAELAYAARTVSGAYARYWEPYLTISVIYWLITLALSALVRHL 240 NNAIAIVKDSSLASAIGLAELAYAARTVSGAYARYWEPYLTISVIYWLITLALSALVRHL Sbjct: 181 NNAIAIVKDSSLASAIGLAELAYAARTVSGAYARYWEPYLTISVIYWLITLALSALVRHL 240 Query: 241 EARYGKGDAR 250 EARYGKGDAR Sbjct: 241 EARYGKGDAR 250 >gi|300313611|ref|YP_003777703.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 247 Score = 301 bits (770), Expect = 5e-83, Method: Compositional matrix adjust. Identities = 149/246 (60%), Positives = 194/246 (78%), Gaps = 1/246 (0%) Query: 5 FQWEIIGEYLPLFVEGTWMTLKAAIICVIAGTCWGLVLGVGRLAEARHGFWKYFLCYCVQ 64 F+W II Y PLF +G MTL+ ++I ++ GT GL++G+ RLAE +HG WK+ L ++ Sbjct: 3 FRWSIIQGYAPLFAKGVLMTLQVSLISILIGTVIGLLVGMLRLAEVQHGPWKWPLK-SMR 61 Query: 65 WPIRFYVSFLRGTPLFVQILLIHFALMPMLINPSGGLILSGDLAREIRSHYGAFASAVLA 124 W FYV+F RGTPLFVQI+LIHFA+MP+L+ GL+++G+LAR I+ YGAF S +A Sbjct: 62 WLAGFYVAFFRGTPLFVQIMLIHFAVMPVLVQQEHGLLITGELARTIKQDYGAFLSGTVA 121 Query: 125 ITLNSGAYVSEIFRAGIQSIDRGQSEASRSLGMSYLATLRRVVLPQAFRRMLPPLGNNAI 184 ++LN+GAY+SEIFRAGIQSI+RGQS A+ SLGM+Y T+R VVLPQAFRRMLPPLGN AI Sbjct: 122 LSLNAGAYISEIFRAGIQSIERGQSYAAASLGMNYGLTMRYVVLPQAFRRMLPPLGNEAI 181 Query: 185 AIVKDSSLASAIGLAELAYAARTVSGAYARYWEPYLTISVIYWLITLALSALVRHLEARY 244 ++KDSSL SAIGLAELAYAARTV+G YARYWEPY+TISV+Y+ +T+ L+ +V LE++Y Sbjct: 182 TLLKDSSLVSAIGLAELAYAARTVAGTYARYWEPYITISVLYFSMTICLALVVARLESKY 241 Query: 245 GKGDAR 250 R Sbjct: 242 SAPHRR 247 >gi|300311471|ref|YP_003775563.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 221 Score = 131 bits (330), Expect = 5e-32, Method: Compositional matrix adjust. Identities = 78/241 (32%), Positives = 121/241 (50%), Gaps = 28/241 (11%) Query: 8 EIIGEYLPLFVEGTWMTLKAAIICVIAGTCWGLVLGVGRLAEARHGFWKYFLCYCVQWPI 67 E++ + P +G TL A+ ++ G G VL V R+ R + WP Sbjct: 7 ELLQQAAPTLAKGVGYTLLFALASMLGGLVLGFVLAVARIVPWRP----------IHWPA 56 Query: 68 RFYVSFLRGTPLFVQILLIHFALMPMLINPSGGLILSGDLAREIRSHYGAFASAVLAITL 127 YVS +RGTPL VQI ++++ L P G+ + + VL ++L Sbjct: 57 AIYVSMMRGTPLLVQIFVVYYGL------PGMGI------------EFSPLTAGVLTLSL 98 Query: 128 NSGAYVSEIFRAGIQSIDRGQSEASRSLGMSYLATLRRVVLPQAFRRMLPPLGNNAIAIV 187 N+ AY+SE R I + RGQ A+ S+G++Y TLR +++PQA R +P + N I+++ Sbjct: 99 NASAYLSESLRGAILGVTRGQWNAAFSVGLTYFQTLRYIIVPQAVRIAVPSMSNTLISLI 158 Query: 188 KDSSLASAIGLAELAYAARTVSGAYARYWEPYLTISVIYWLITLALSALVRHLEARYGKG 247 KD+SL S I + EL A + V R Y+ + IYW+++L AL LE + GK Sbjct: 159 KDTSLVSVITVTELMLATKEVIAVTFRPLPLYVAAAAIYWMLSLCFEALQHRLEKKLGKA 218 Query: 248 D 248 Sbjct: 219 H 219 >gi|300310495|ref|YP_003774587.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 218 Score = 119 bits (298), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 81/239 (33%), Positives = 131/239 (54%), Gaps = 28/239 (11%) Query: 12 EYLPLFVEGTWMTLKAAIICVIAGTCWGLVLGVGRLAEARHGFWKYFLCYCVQWPIRFYV 71 ++LP+ ++G +T++ I + + GL+L + +++ + V V Sbjct: 8 DFLPILLKGALVTVEVTIYSFLLSSVIGLLLALMKVSRLKP----------VALAGATIV 57 Query: 72 SFLRGTPLFVQILLIHFALMPMLINPSGGLILSGDLAREIRSHYGAFASAVLAITLNSGA 131 + +RG P+ VQ+ I+F L P G+ LS AF + V+ + + A Sbjct: 58 NIIRGLPIIVQLFYIYFVL------PDIGIQLS------------AFQAGVVGLGIAYSA 99 Query: 132 YVSEIFRAGIQSIDRGQSEASRSLGMSYLATLRRVVLPQAFRRMLPPLGNNAIAIVKDSS 191 Y +E FRAGI+++D GQ EA++S+GM +RRVVLPQAFR LPP GN + ++KDSS Sbjct: 100 YQAENFRAGIEAVDMGQIEAAQSIGMRGPLIMRRVVLPQAFRIALPPYGNTLVMMLKDSS 159 Query: 192 LASAIGLAELAYAARTVSGAYARYWEPYLTISVIYWLITLALSALVRHLEARYGKGDAR 250 L S I + E+ A + ++ + + Y T++V+Y L++L L L+R LE R KG AR Sbjct: 160 LVSTITVLEMTRAGQMIASSTFQNMTVYTTVAVLYLLLSLPLVFLIRRLEIRNSKGRAR 218 >gi|300311602|ref|YP_003775694.1| glutamine ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 221 Score = 117 bits (292), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 74/173 (42%), Positives = 105/173 (60%), Gaps = 18/173 (10%) Query: 69 FYVSFLRGTPLFVQILLIHFALMPMLINPSGGLILSGDLAREIRSHYGAFASAVLAITLN 128 Y++ +RGTPL VQ+ L F L P G+IL A+ +L + + Sbjct: 58 IYLTLVRGTPLLVQLFLWFFGL------PHVGVILP------------AYVCGILGLGIY 99 Query: 129 SGAYVSEIFRAGIQSIDRGQSEASRSLGMSYLATLRRVVLPQAFRRMLPPLGNNAIAIVK 188 SGAYVSEI R IQSI+RGQ EA+RSLG+ Y +RRV+LPQAF RM+PPLGN IA++K Sbjct: 100 SGAYVSEIVRGAIQSIERGQMEAARSLGLPYRMAMRRVILPQAFVRMIPPLGNEFIALIK 159 Query: 189 DSSLASAIGLAELAYAARTVSGAYARYWEPYLTISVIYWLITLALSALVRHLE 241 +S+L S + +A+L + + R E YL I++IY L+T + ++R +E Sbjct: 160 NSALVSLLTIADLMHEGEKIISVTYRSLETYLAIALIYLLLTSVTTLILRRVE 212 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.141 0.441 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 794,182 Number of extensions: 32668 Number of successful extensions: 193 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 175 Number of HSP's successfully gapped: 19 Length of query: 250 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 167 Effective length of database: 1,226,865 Effective search space: 204886455 Effective search space used: 204886455 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 129 (54.3 bits)