BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0044.p (299 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309409|ref|YP_003773501.1| glutamine ABC transporter perip... 520 e-149 gi|300313610|ref|YP_003777702.1| amino acid ABC transporter/sign... 204 6e-54 gi|300312810|ref|YP_003776902.1| glutamine ABC transporter/signa... 125 5e-30 gi|300311472|ref|YP_003775564.1| amino acid ABC transporter/sign... 104 7e-24 gi|300310494|ref|YP_003774586.1| amino acid ABC transporter/sign... 84 2e-17 >gi|300309409|ref|YP_003773501.1| glutamine ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 259 Score = 520 bits (1340), Expect = e-149, Method: Compositional matrix adjust. Identities = 259/259 (100%), Positives = 259/259 (100%) Query: 41 MSLKKLLTTAASALALSCAAGTALAADQTYVVGAGGTYRPFEFETPKKELVGFDIDLIKA 100 MSLKKLLTTAASALALSCAAGTALAADQTYVVGAGGTYRPFEFETPKKELVGFDIDLIKA Sbjct: 1 MSLKKLLTTAASALALSCAAGTALAADQTYVVGAGGTYRPFEFETPKKELVGFDIDLIKA 60 Query: 101 ISQASNFKIQLVSTPWEGIFATLGQGDRDIIISGITITDKRAQMVDFSLPYFPAEQVIIT 160 ISQASNFKIQLVSTPWEGIFATLGQGDRDIIISGITITDKRAQMVDFSLPYFPAEQVIIT Sbjct: 61 ISQASNFKIQLVSTPWEGIFATLGQGDRDIIISGITITDKRAQMVDFSLPYFPAEQVIIT 120 Query: 161 NADAKITSLSDLKKTQVAVVNSTAGDIVVSEELGKASTAIHRFDNTVLMLEELYRGGVDA 220 NADAKITSLSDLKKTQVAVVNSTAGDIVVSEELGKASTAIHRFDNTVLMLEELYRGGVDA Sbjct: 121 NADAKITSLSDLKKTQVAVVNSTAGDIVVSEELGKASTAIHRFDNTVLMLEELYRGGVDA 180 Query: 221 AVGDVGVIKFYIKSHPEKKFKVVGDSKFVRQYFGIAVKKGNKELLDKINAGLKKIVADGT 280 AVGDVGVIKFYIKSHPEKKFKVVGDSKFVRQYFGIAVKKGNKELLDKINAGLKKIVADGT Sbjct: 181 AVGDVGVIKFYIKSHPEKKFKVVGDSKFVRQYFGIAVKKGNKELLDKINAGLKKIVADGT 240 Query: 281 YAKIYKTWFDGDVPTLPLK 299 YAKIYKTWFDGDVPTLPLK Sbjct: 241 YAKIYKTWFDGDVPTLPLK 259 >gi|300313610|ref|YP_003777702.1| amino acid ABC transporter/signal transduction system periplasmic protein/domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 271 Score = 204 bits (520), Expect = 6e-54, Method: Compositional matrix adjust. Identities = 105/223 (47%), Positives = 147/223 (65%), Gaps = 1/223 (0%) Query: 68 QTYVVGAGGTYRPFEFETPKKELVGFDIDLIKAISQASNFKIQLVSTPWEGIFATLGQGD 127 + YVVG Y PF E +K++VGFDID++KA+++ +++ V TP+EG F L QGD Sbjct: 47 RVYVVGVESAYAPFSSENEQKDVVGFDIDIMKALAKKIGIEVKFVPTPFEGFFNFLAQGD 106 Query: 128 RDIIISGITITDKRAQMVDFSLPYFPAEQVI-ITNADAKITSLSDLKKTQVAVVNSTAGD 186 RD++IS ITITD+R + V FS PYF A Q I + AD K++ + DLK V ++T+GD Sbjct: 107 RDLLISAITITDERKKSVAFSEPYFVATQTIALPAADTKVSKMEDLKPLTVGTQSATSGD 166 Query: 187 IVVSEELGKASTAIHRFDNTVLMLEELYRGGVDAAVGDVGVIKFYIKSHPEKKFKVVGDS 246 +V + LGK S I RFD+T L L+EL GGVDA V D V+K YI ++P K + V D Sbjct: 167 ELVQQVLGKNSAKIKRFDSTPLALKELESGGVDAVVADEPVVKNYIANNPNSKLRTVTDP 226 Query: 247 KFVRQYFGIAVKKGNKELLDKINAGLKKIVADGTYAKIYKTWF 289 F ++ +GIAV+K + ELL KIN GL ++ ADG++A I +F Sbjct: 227 SFPKEDYGIAVRKDDPELLAKINKGLAEMKADGSFAAISAQYF 269 >gi|300312810|ref|YP_003776902.1| glutamine ABC transporter/signal transduction system periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 249 Score = 125 bits (314), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 88/247 (35%), Positives = 134/247 (54%), Gaps = 13/247 (5%) Query: 51 ASALALSCAAGTALAADQTYVVGAGGTYRPFEFETPKKELVGFDIDLIKAISQASNFKIQ 110 AS +ALS +A A +T VV + PFEF+ + + VGFDIDL AI++ + Sbjct: 11 ASLVALSVVGASARA--ETLVVACDTAFVPFEFKD-RNQYVGFDIDLWDAIAKDIGVTYK 67 Query: 111 LVSTPWEGIFATLGQGDRDIIISGITITDKRAQMVDFSLPYFPAEQVIITNADAKITSLS 170 L + GI L + D+ ++GITI D+R +++DFS Y+ + ++ + I S++ Sbjct: 68 LQPMDFNGIIPALQTKNVDVALAGITIKDERKKVIDFSDGYYDSGFSLMVPVGSSIKSVA 127 Query: 171 DLKKTQVAVVNSTAGDIVVSEELGKA---STAIHRFDNTVLMLEELYRGGVDAAVGDVGV 227 DL +AV + T+ + + KA +T +H+F N EL G VDAA+ D Sbjct: 128 DLAGKALAVKSGTS-----AFDYAKANFKATELHQFPNIDNAYLELQTGRVDAAMHDTPN 182 Query: 228 IKFYIKSHPEKKFKVVGDSKFVRQYFGIAVKKGNKELLDKINAGLKKIVADGTYAKIYKT 287 + +YI + + + KVVG QY GI KG+ L+ K+NA L KI ADG YA+IY+ Sbjct: 183 VLYYIATAGKGRAKVVGTQMMAHQY-GIGFPKGS-PLVPKVNAALAKIKADGRYAEIYRK 240 Query: 288 WFDGDVP 294 WF + P Sbjct: 241 WFGAEPP 247 >gi|300311472|ref|YP_003775564.1| amino acid ABC transporter/signal transduction system periplasmic protein/domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 267 Score = 104 bits (260), Expect = 7e-24, Method: Compositional matrix adjust. Identities = 83/255 (32%), Positives = 122/255 (47%), Gaps = 17/255 (6%) Query: 51 ASALALSCA-AGTALAADQTYVVGAGGTYRPFEFETPKK-ELVGFDIDLIKAISQASNFK 108 AS+L++S A ++ + T V G Y PF F+ PK EL GF++D+ K ++ K Sbjct: 19 ASSLSVSAADLLQSVKSSGTLKVALEGNYPPFNFKDPKTGELTGFEVDVAKLLAAKLGVK 78 Query: 109 IQLVSTPWEGIFATLGQGDRDIIISGITITDKRAQMVDFSLPYFPAEQVIITNADAK--I 166 +T W GI A LG G D+II+ + ITD+R + DFS PY + +I D K Sbjct: 79 PVFTTTEWSGILAGLGAGKYDVIINQVGITDERQKAFDFSDPYTLSSAQLIVRKDEKREF 138 Query: 167 TSLSDLKKTQVAVVNSTAGDIVVSEELGKASTAI--HRFDNTVLMLEELYRGGVDAAVGD 224 SL DLK ++ + T E+ KA I + + L +L G +DAA+ D Sbjct: 139 KSLEDLKGKKLGLGQGTN-----FEQKAKAVPGIDVRTYPGSPEYLADLAAGRIDAALND 193 Query: 225 VGVIKFYIKSH--PEKKFKVVGDSKFVRQYFGIAVKKGNKELLDKINAGLKKIVADGTYA 282 ++ + +KS P K +G GI +KGN E +N L I ADG++ Sbjct: 194 SLLVGYILKSTNLPLKAGSPIG----AVDKIGIPFRKGNPEFKAALNKALADIKADGSFK 249 Query: 283 KIYKTWFDGDVPTLP 297 + WF DV P Sbjct: 250 AASEKWFGIDVSQPP 264 >gi|300310494|ref|YP_003774586.1| amino acid ABC transporter/signal transduction system periplasmic protein/domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 258 Score = 84.0 bits (206), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 61/231 (26%), Positives = 115/231 (49%), Gaps = 14/231 (6%) Query: 66 ADQTYVVGAGGTYRPFEF-ETPKKELVGFDIDLIKAISQASNFKIQLVSTPWEGIFATLG 124 A +Y VGA T PF F + +L G +D + A+ + F + + TP+ + +L Sbjct: 31 AQASYNVGATSTGVPFTFLDIKTNKLQGMMVDTVTAVGNKAGFSVNIQQTPFAALIPSLT 90 Query: 125 QGDRDIIISGITITDKRAQMVDFSLPYFPAEQVIITNA-DAK-ITSLSDLKKTQVAVVNS 182 DII + + T+ RAQ+VDFS P + + + NA D K +L DLK +V + Sbjct: 91 GNKIDIIAAAMLKTEARAQIVDFSDPVYSYGEGLFVNAQDGKSYKNLDDLKGE---IVGA 147 Query: 183 TAGDIVVSEELGKAS--TAIHRFDNTVLMLEELYRGGVDAAVGDVGVIKFYIKSHPEKKF 240 G + V + L K + +D+ M+ ++ G + A GD ++ + + + Sbjct: 148 QVGTVFV-DALNKKGGFKEVKTYDSVADMMRDVALGRIKAGFGDRPIVAYQLAQGANPQV 206 Query: 241 KVVGDSKFVRQYFG---IAVKKGNKELLDKINAGLKKIVADGTYAKIYKTW 288 ++V ++ G + ++KG+ +L++++N + ++ ADGT A+I + W Sbjct: 207 RLV--KEYQPTLMGDVCLVIRKGDPKLMERLNKAIAELKADGTLARIVEQW 255 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 789,050 Number of extensions: 26447 Number of successful extensions: 103 Number of sequences better than 1.0e-08: 15 Number of HSP's gapped: 91 Number of HSP's successfully gapped: 15 Length of query: 299 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 214 Effective length of database: 1,217,395 Effective search space: 260522530 Effective search space used: 260522530 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)