BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0038.p (288 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309401|ref|YP_003773493.1| nitrate/sulfonate/bicarbonate A... 558 e-160 gi|300311840|ref|YP_003775932.1| nitrate/sulfonate/bicarbonate A... 323 7e-90 gi|300309716|ref|YP_003773808.1| nitrate/sulfonate/bicarbonate A... 91 1e-19 gi|300310979|ref|YP_003775071.1| alkanesulfonates ABC transporte... 83 2e-17 gi|300309985|ref|YP_003774077.1| nitrate/sulfonate/bicarbonate A... 70 1e-13 >gi|300309401|ref|YP_003773493.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 288 Score = 558 bits (1437), Expect = e-160, Method: Compositional matrix adjust. Identities = 287/288 (99%), Positives = 287/288 (99%) Query: 1 MAQTPPIRDEYEIPLTPLPGLVLQRQFSLPQRLWQQGWVRKGAILLLLALVWEGVARWQN 60 MAQTPPIRDEYEIPLTPLPGLVLQRQFSLPQRLWQQGWVRKGAILLLLALVWEGVARWQN Sbjct: 1 MAQTPPIRDEYEIPLTPLPGLVLQRQFSLPQRLWQQGWVRKGAILLLLALVWEGVARWQN 60 Query: 61 NDLLLPSFLQTAQAFIDGLRSGELFGKTWLSLQVLLKAYVLGIVAAFALTTLAVSTQFGR 120 NDLLLPSFLQTAQAFIDGLRSGELFGKTWLSLQVLLKAYVLGIVAAFALTTLAVSTQFGR Sbjct: 61 NDLLLPSFLQTAQAFIDGLRSGELFGKTWLSLQVLLKAYVLGIVAAFALTTLAVSTQFGR 120 Query: 121 DLLGTLTSMFNPLPAIALLPIALLWFGLGQGSLIFVLVHSVLWALAVNTYAGFLGVSDTL 180 DLLGTLTSMFNPLPAIALLPIALLWFGLGQGSLIFVLVHSVLWALAVNTYAGFLGVSDTL Sbjct: 121 DLLGTLTSMFNPLPAIALLPIALLWFGLGQGSLIFVLVHSVLWALAVNTYAGFLGVSDTL 180 Query: 181 RMAGRNYGLRGLGFVLQVLVPAALPSILAGLRIGWAFAWRTLIAAELVFGATSGKGGLGW 240 RMAGRNYGLRGLGFVLQVLVPAALPSILAGLRIGWAFAWRTLIAAELVFGATSGKGGLGW Sbjct: 181 RMAGRNYGLRGLGFVLQVLVPAALPSILAGLRIGWAFAWRTLIAAELVFGATSGKGGLGW 240 Query: 241 YIFQNRNELYTDKVFACLAMVILIGLLVENLVFNTLERVTVKKWGMQR 288 YIFQNRNELYTDKVFA LAMVILIGLLVENLVFNTLERVTVKKWGMQR Sbjct: 241 YIFQNRNELYTDKVFAGLAMVILIGLLVENLVFNTLERVTVKKWGMQR 288 >gi|300311840|ref|YP_003775932.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 289 Score = 323 bits (829), Expect = 7e-90, Method: Compositional matrix adjust. Identities = 168/284 (59%), Positives = 213/284 (75%) Query: 5 PPIRDEYEIPLTPLPGLVLQRQFSLPQRLWQQGWVRKGAILLLLALVWEGVARWQNNDLL 64 P +R E + P +QR S + L++ GW+RK ILL+LA +WE RW +N LL Sbjct: 6 PFVRPEILVDPNPHATAEVQRPLSAFESLYRMGWLRKTVILLVLAAIWEVYGRWLDNALL 65 Query: 65 LPSFLQTAQAFIDGLRSGELFGKTWLSLQVLLKAYVLGIVAAFALTTLAVSTQFGRDLLG 124 PSF +T QAF+ G+ SG L + +S+Q LL Y LGI+ A LTT+A+S++ G DLL Sbjct: 66 FPSFSETVQAFLSGIGSGVLLQRAAVSMQTLLVGYGLGILLAGLLTTVAISSRIGNDLLE 125 Query: 125 TLTSMFNPLPAIALLPIALLWFGLGQGSLIFVLVHSVLWALAVNTYAGFLGVSDTLRMAG 184 TLTSMFNPLPAIALLP+AL+WFGLG GS+IFVLVHSVLWA+A+NT+ GF VS+TLRM G Sbjct: 126 TLTSMFNPLPAIALLPLALIWFGLGTGSIIFVLVHSVLWAVALNTHGGFRAVSNTLRMVG 185 Query: 185 RNYGLRGLGFVLQVLVPAALPSILAGLRIGWAFAWRTLIAAELVFGATSGKGGLGWYIFQ 244 +NYGLR V +L+PAA PSIL GL++GWAFAWRTLIAAELVFG +SG GGLGWYIF+ Sbjct: 186 QNYGLRRFSLVKSILIPAAFPSILTGLKVGWAFAWRTLIAAELVFGVSSGAGGLGWYIFE 245 Query: 245 NRNELYTDKVFACLAMVILIGLLVENLVFNTLERVTVKKWGMQR 288 NRN+L T VF+ L VI+IGL+VENL+F +E+ T+++WGMQ Sbjct: 246 NRNQLETANVFSGLFFVIIIGLVVENLIFAQIEKRTIRRWGMQH 289 >gi|300309716|ref|YP_003773808.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 273 Score = 90.9 bits (224), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 69/235 (29%), Positives = 117/235 (49%), Gaps = 8/235 (3%) Query: 35 QQGWVRKGAILLLLALVWEGVARWQNNDLLLPSFLQTAQAFIDGLRSGELFGKTWLSLQV 94 + GW+ +L W + +W L + A +++ SGEL SL Sbjct: 28 RAGWINPQ----ILPSPWAVLRKWVEYATPLKPYDPEAGSWLAWAFSGELVMDAIGSLYR 83 Query: 95 LLKAYVLGIVAAFALTTLAVSTQFGRDLLGTLTSMFNPLPAIALLPIALLWFGLGQGSLI 154 ++ +++G A L L S+Q L+ + P+P IA +P+A+LWFGLG + Sbjct: 84 VVAGFLIGAGLALPLGLLMGSSQRIYGLMNLTVQVIRPIPPIAYIPLAILWFGLGNPPAL 143 Query: 155 FVLVHSVLWALAVNTYAGFLGVSDTLRMAGRNYGLRGLGFVLQVLVPAALPSILAGLRIG 214 F++ + + +NT AG V A RN G L+V++PAA+P IL+G+RIG Sbjct: 144 FLISLGAFFPVLMNTIAGVRQVDSIYLRAARNLGASQSTLFLRVMLPAAVPYILSGVRIG 203 Query: 215 WAFAWRTLIAAELVFGATSGKGGLGWYIFQNRNELYTDKVFACLAMVILIGLLVE 269 A+ +I AE++ + GLG+ I + R ++DK+ A + + L+GL ++ Sbjct: 204 IGTAFIVVIVAEMI----AVSNGLGFRIMEAREYFWSDKIIAGMITIGLLGLAID 254 >gi|300310979|ref|YP_003775071.1| alkanesulfonates ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 279 Score = 83.2 bits (204), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 50/149 (33%), Positives = 78/149 (52%), Gaps = 4/149 (2%) Query: 122 LLGTLTSMFNPLPAIALLPIALLWFGLGQGSLIFVLVHSVLWALAVNTYAGFLGVSDTLR 181 LL T M +PA+AL+P+ +LWFG+ + + +F++ V + + +NT+ G V L Sbjct: 118 LLDTTLQMVRNIPALALIPLVILWFGIDETAKLFLVSVGVFFPVYLNTFHGIRSVDQGLI 177 Query: 182 MAGRNYGLRGLGFVLQVLVPAALPSILAGLRIGWAFAWRTLIAAELVFGATSGKGGLGWY 241 ++YGL G V++P ALP+IL GLR W LI AE + S + G+G+ Sbjct: 178 EMAKSYGLSGWPLYRDVILPGALPAILVGLRFALGLVWVLLIVAETI----SAQAGIGYM 233 Query: 242 IFQNRNELYTDKVFACLAMVILIGLLVEN 270 R L TD V + + L+G L + Sbjct: 234 TMNAREFLQTDVVLVGILLYALLGKLADT 262 >gi|300309985|ref|YP_003774077.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 278 Score = 70.5 bits (171), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 67/237 (28%), Positives = 109/237 (45%), Gaps = 9/237 (3%) Query: 37 GWVRKGAILLLLALVWEGVARWQ-NNDLLLPSFLQTAQAFIDGLRSGELFGKTWLSLQVL 95 GW+ A LLL+ E AR + LLP + A + GEL SL + Sbjct: 28 GWIVPLAFLLLV----EAGARLELIPARLLPPPSELAHTLWSLAQRGELLPHIAASLARV 83 Query: 96 LKAYVLGIVAAFALTTLAVSTQFGRDLLGTLTSMFNPLPAIALLPIALLWFGLGQGSLIF 155 +V+G A AL L ++ LL +P++A +P+ LLW G+ + + Sbjct: 84 AAGFVIGSALAVALGLLVGISRRAEALLEPSFQALRAIPSLAWIPLLLLWLGIDETPKVV 143 Query: 156 VLVHSVLWALAVNTYAGFLGVSDTLRMAGRNYGLRGLGFVLQVLVPAALPSILAGLRIGW 215 ++ + + +N AG + L G GL + +L+PA+LP + AGLR G Sbjct: 144 LIAIGAFFPVYLNLVAGIRDIDHKLIEVGTTSGLNTQALIGHILLPASLPYLFAGLRGGL 203 Query: 216 AFAWRTLIAAELVFGATSGKGGLGWYIFQNRNELYTDKVFACLAMVILIGLLVENLV 272 + AW L+AAEL+ + G+G+ + R D V A + ++ L+G L + L+ Sbjct: 204 SLAWMFLVAAELI----AATKGVGYLLSDGRESSRPDLVLAAILILALLGKLSDGLL 256 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.142 0.448 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 926,213 Number of extensions: 39435 Number of successful extensions: 222 Number of sequences better than 1.0e-08: 16 Number of HSP's gapped: 214 Number of HSP's successfully gapped: 16 Length of query: 288 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 204 Effective length of database: 1,222,130 Effective search space: 249314520 Effective search space used: 249314520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 130 (54.7 bits)