BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0016.p (338 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309380|ref|YP_003773472.1| Fe2+-dicitrate sensor membrane ... 645 0.0 gi|300313764|ref|YP_003777856.1| Fe2+-dicitrate sensor membrane ... 179 4e-46 gi|300311747|ref|YP_003775839.1| Fe2+-dicitrate sensor membrane ... 123 2e-29 gi|300312829|ref|YP_003776921.1| Fe2+-dicitrate sensor membrane ... 111 7e-26 gi|300313795|ref|YP_003777887.1| transmembrane sensor protein [H... 92 5e-20 >gi|300309380|ref|YP_003773472.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 336 Score = 645 bits (1665), Expect = 0.0, Method: Compositional matrix adjust. Identities = 331/338 (97%), Positives = 332/338 (98%), Gaps = 2/338 (0%) Query: 1 LAHSSWYQPTADAADVPPQVAERALEWLLELQTQPLAPSTLEAWEAWRSAHPDHERAWRR 60 +AHSSWYQPTADAADVPPQVAERALEWLLELQTQPLAPSTLEAWEAWRSAHPDHERAWRR Sbjct: 1 MAHSSWYQPTADAADVPPQVAERALEWLLELQTQPLAPSTLEAWEAWRSAHPDHERAWRR 60 Query: 61 IESVRGRLQALASPVNAALAQATLTPAPSGNRRQVIKTLALLVFGGGVAWGLTRSEPWQQ 120 IESVRGRLQALASPVNAALAQA LTP SGNRRQVIKTLALLVFGGGVAWGLTRSEPWQQ Sbjct: 61 IESVRGRLQALASPVNAALAQAALTP--SGNRRQVIKTLALLVFGGGVAWGLTRSEPWQQ 118 Query: 121 WRADLRTDIGQQRRLVLEDGSQLVLNTHSAVNLAFSAEERRLRLISGELLVTTGKAGGGR 180 WRADLRTDIGQQRRLVLEDGSQLVLNTHSAVNLAFSAEERRLRLISGELLVTTGKAGGGR Sbjct: 119 WRADLRTDIGQQRRLVLEDGSQLVLNTHSAVNLAFSAEERRLRLISGELLVTTGKAGGGR 178 Query: 181 PFLVETAQGLAYALGTRYAVRQRAGRTEVSVWEGAVRLVPRQAAQQALVLQAGQQATFSA 240 PFLVETAQGLAYALGTRYAVRQRAGRTEVSVWEGAVRLVPRQAAQQA VLQAGQQA FSA Sbjct: 179 PFLVETAQGLAYALGTRYAVRQRAGRTEVSVWEGAVRLVPRQAAQQARVLQAGQQAAFSA 238 Query: 241 QGVGDVTTADETRMAWTDGVIVARGMRLDAFLAELGRYTTDRLSCAPAVSGVRISGSYPV 300 QGVGDVTTADETRMAWTDGVIVARGMRLDAFLAELGRYTTDRLSCAPAVSGVRISGSYPV Sbjct: 239 QGVGDVTTADETRMAWTDGVIVARGMRLDAFLAELGRYTTDRLSCAPAVSGVRISGSYPV 298 Query: 301 ADSDAVIATLAAALGLQAQLRQRFWGARQWYLAPSPDA 338 ADSDAVIATLAAALGLQAQLRQRFWG RQWYLAPSPDA Sbjct: 299 ADSDAVIATLAAALGLQAQLRQRFWGGRQWYLAPSPDA 336 >gi|300313764|ref|YP_003777856.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 326 Score = 179 bits (453), Expect = 4e-46, Method: Compositional matrix adjust. Identities = 115/304 (37%), Positives = 166/304 (54%), Gaps = 8/304 (2%) Query: 24 ALEWLLELQTQPLAPSTLEAWEAWRSAHPDHERAWRRIESVRGRLQALASPVNAALAQAT 83 A WL+E+ PL + WR P+HERAW R ++ AL P A A Sbjct: 19 AATWLMEMHEGPLDAARRSKLAQWRQRSPEHERAWSRAALLQETFAAL--PPGGATALKQ 76 Query: 84 LTPAPSGNRRQVIKTLALLVFGGGVAWGLTRSEPWQQWRADLRTDIGQQRRLVLEDGSQL 143 + PA RR+ IK LA + G W + + A + T IG++R + L+DGS+L Sbjct: 77 VGPA---GRRKAIKLLAYALTLGPAGWLAYHALSGPEQGARIATAIGERREVQLDDGSRL 133 Query: 144 VLNTHSAVNLAFSAEERRLRLISGELLVTTG--KAGGGRPFLVETAQGLAYALGTRYAVR 201 LNT + + L F+ +R LRL GE++V T + RPFLV T QG LGTR+ VR Sbjct: 134 WLNTDTRITLDFTPAQRLLRLQRGEIMVLTAPDRQQPPRPFLVATRQGSLRPLGTRFTVR 193 Query: 202 QRAGRTEVSVWEGAVRLVPRQAAQQALVLQAGQQATFSAQGVGDVTTADETRMAWTDGVI 261 +R EV+V +GAV + P +AQQ VL +G Q F+ G D+T+ AW+ G++ Sbjct: 194 ERDQAVEVAVLQGAVEITPASSAQQT-VLPSGMQTRFTRSGSAAPRPCDDTQAAWSRGLL 252 Query: 262 VARGMRLDAFLAELGRYTTDRLSCAPAVSGVRISGSYPVADSDAVIATLAAALGLQAQLR 321 +A M L F+ ELGRY + L PA++G+ +SG +P+AD+D V+ LA L ++ Q Sbjct: 253 IAERMPLAQFIGELGRYRSGLLQADPAIAGLEVSGVFPLADTDRVLRLLAQTLPVRVQQL 312 Query: 322 QRFW 325 R+W Sbjct: 313 TRYW 316 >gi|300311747|ref|YP_003775839.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 342 Score = 123 bits (308), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 110/331 (33%), Positives = 151/331 (45%), Gaps = 34/331 (10%) Query: 20 VAERALEWLLELQTQP----LAPSTLEAWEAWRSAHPDHERAWRRI---ESVRGRLQALA 72 V ++A+ WL++L+ P +AP AWR+A P HE+ W + E RL AL Sbjct: 12 VLDQAIAWLVKLRAFPGDAAVAPELQAGIAAWRAADPRHEQVWSELMASEEQFQRLVALP 71 Query: 73 SP----------VNAALAQATLTPAPSGNRRQVIKTLALLVFGGGVAWGLTRSEPWQQWR 122 P +N + GN + +AL A GL + Sbjct: 72 VPASQWHGPLKRLNQRRQHSQSRRRWMGNAMLGLGAIALGAVAARQA-GLFEALD----G 126 Query: 123 ADLRTDIGQQRRLVLEDGSQLVLNTHSAVNLAFSAEERRLRLISGELLVTTGKAG---GG 179 A T G QR+L L DG+ L LNT S+V L F R L L GE+ + TG Sbjct: 127 ASYATRTGDQRQLRLGDGTALALNTSSSVQLRFDDTRRLLLLRQGEIFIDTGADAQWPHH 186 Query: 180 RPFLVETAQGLAYALGTRYAVRQRAGRTEVSVWEGAVRLVPRQAAQQALVLQAGQQATFS 239 RPF V+TA ALGTR+ VRQ T+++V EG R+ A LV Q G+ Sbjct: 187 RPFWVQTAHARLQALGTRFDVRQHGDGTQLTVLEG--RVAVHVAGVDPLVAQPGEIVDIH 244 Query: 240 AQG-----VGDVTTADETRMAWTDGVIVARGMRLDAFLAELGRYTTDRLSCAPAVSGVRI 294 G + D T AW + +V R MRL +A+L RY + L C PAV+ ++ Sbjct: 245 PDGRLVRRAARASAMDPT--AWLEQSLVVRQMRLADVVAQLARYRSGWLQCDPAVADWQV 302 Query: 295 SGSYPVADSDAVIATLAAALGLQAQLRQRFW 325 SG + + D+D + LA AL L + R RFW Sbjct: 303 SGVFQLQDTDVALEALAQALPLTIERRTRFW 333 >gi|300312829|ref|YP_003776921.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 316 Score = 111 bits (278), Expect = 7e-26, Method: Compositional matrix adjust. Identities = 94/310 (30%), Positives = 142/310 (45%), Gaps = 9/310 (2%) Query: 19 QVAERALEWLLELQTQPLAPSTLEAWEAWRSAHPDHERAWRRIESVRGRLQALASPVNAA 78 ++ E+A+EW+L+L+ L P A+E W++A P H A+ + +Q P Sbjct: 6 KIVEQAIEWMLQLEQGDLPPGERAAFEQWKAADPRHAMAYANLSQ---SVQQFDVPRQVG 62 Query: 79 LAQATL--TPAPSGNRRQVIKTLALLVFGGGVAWGLT-RSEPWQQWRADLRTDIGQQRRL 135 A L T RR+ +K +A L A L R P ADL T GQ++R Sbjct: 63 AAPVILRNTLQQKSKRRKALKRIAALAGLAVGAAALLDRVTPVAALTADLHTGTGQRQRT 122 Query: 136 VLEDGSQLVLNTHSAVNLAFSAEERRLRLISGELLVTTGKAGGGRPFLVETAQGLAYALG 195 L DGS + LN SA++ S R++RL+ GE+ V A PF +ET G A Sbjct: 123 TLADGSIITLNARSAIDHLISPVLRQVRLLEGEIQVQIA-AANALPFSIETVHGSVSATA 181 Query: 196 TRYAVRQRAGRTEVSVWEGAVRLVPRQAAQQALVLQAGQQATFSAQGVGDVTTADETRMA 255 + + RT++ + A L R Q L AGQ+ F +G A + Sbjct: 182 GTLMLGLQPLRTDLVAQQAAALLSTRNG--QHATLAAGQRTWFDQDEIGAPRAAGGAENS 239 Query: 256 WTDGVIVARGMRLDAFLAELGRYTTDRLSCAPAVSGVRISGSYPVADSDAVIATLAAALG 315 W +G A L+ +A L Y L PAV+ +RISG++P+ D+D ++ LA+AL Sbjct: 240 WVEGYYTASDNSLEEVVAALRPYRKGLLRLDPAVAQLRISGAFPLDDTDRALSALASALP 299 Query: 316 LQAQLRQRFW 325 + +W Sbjct: 300 ITVNRVSPYW 309 >gi|300313795|ref|YP_003777887.1| transmembrane sensor protein [Herbaspirillum seropedicae SmR1] Length = 342 Score = 92.4 bits (228), Expect = 5e-20, Method: Compositional matrix adjust. Identities = 84/276 (30%), Positives = 129/276 (46%), Gaps = 14/276 (5%) Query: 47 WRSAHPDHERAWRRIESVRGRLQAL-------ASPVNAALAQATLTPAPSGNRRQVIKTL 99 W+ H +A R ++ V + L SP+ A L +A + P+P R + Sbjct: 44 WQGQSRQHAQAARELQGVLAHCEGLLHSAQGDPSPLLAGL-EAAMAPSPRPGRGRRAVAA 102 Query: 100 ALLVFGGGVA-WGLTRSEPWQQWRADLRTDIGQQRRLVLEDGSQLVLNTHSAVNLAFSAE 158 L G+ W ++ P + AD+RT G L DGS+L+L+ SA N F A+ Sbjct: 103 LALACCIGLPLWLGLQAYPARYLLADMRTATGHWDARTLADGSRLLLSGSSAANFHFDAQ 162 Query: 159 ERRLRLISGELLVTTGKAGGGRPFLVETAQGLAYALGTRYAVRQRAGRTEVSVWEGAVRL 218 R + L+ GELLV + RPF+++TA G ALGTR+ VR+ T VS+ E V + Sbjct: 163 RRTVELVQGELLVEVAR-DPARPFVIQTADGSIRALGTRFLVRRDRAGTLVSMLESKVSI 221 Query: 219 VPRQAAQQA-LVLQAGQQATFSAQGVGDVTTADETRM--AWTDGVIVARGMRLDAFLAEL 275 P QA LVL+AGQ+ G + D + AW +V+ L L EL Sbjct: 222 QPGADTTQAPLVLEAGQRVRLHGGAAGPLEHIDAAALERAWRRHQLVSNARPLTEVLDEL 281 Query: 276 GRYTTDRLSCAPA-VSGVRISGSYPVADSDAVIATL 310 R+ R+ A ++G+ ++ P+ D+ + L Sbjct: 282 NRHRPGRILYDQAQLAGLHMTVVLPLDDTQRALQLL 317 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.130 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,012,146 Number of extensions: 38252 Number of successful extensions: 205 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 195 Number of HSP's successfully gapped: 6 Length of query: 338 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 252 Effective length of database: 1,212,660 Effective search space: 305590320 Effective search space used: 305590320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)