BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0008.p (549 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [H... 1086 0.0 gi|300310257|ref|YP_003774349.1| acetyl-coenzyme A synthetase [H... 266 3e-72 gi|300310875|ref|YP_003774967.1| acetyl-coenzyme A synthetase [H... 193 3e-50 gi|300312417|ref|YP_003776509.1| propionyl-CoA ligase [Herbaspir... 168 1e-42 gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligas... 155 1e-38 >gi|300309365|ref|YP_003773457.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 549 Score = 1086 bits (2809), Expect = 0.0, Method: Compositional matrix adjust. Identities = 532/542 (98%), Positives = 534/542 (98%) Query: 8 RSPITAAHYHPAQPTPYPWDIPTHFNIAQAACDRWADGSGRTAIICEDADGSVTSYSYDQ 67 RS I+AAHY P+PY W IPTHFNIAQAACDRWADGSGRTAIICEDADGSVTSYSYDQ Sbjct: 8 RSVISAAHYRAGNPSPYEWQIPTHFNIAQAACDRWADGSGRTAIICEDADGSVTSYSYDQ 67 Query: 68 LKILSDRFANALRAAGVGRGDRIGIYLSQRIETVITHLAAYKLGAITVPLFYLFGPDAIA 127 LKILSDRFANALRAAGVGRGDRIGIYLSQRIETVITHLAAYKLGAITVPLFYLFGPDAIA Sbjct: 68 LKILSDRFANALRAAGVGRGDRIGIYLSQRIETVITHLAAYKLGAITVPLFYLFGPDAIA 127 Query: 128 YRLDNSGAVALVTDASGMEKATLAGELPALRLVFCVESSDLVLPQTTDFWDRLHAAPAEL 187 YRLDNSGAVALVTDASGMEKATLAGELPALRLVFCVESSDLVLPQTTDFWDRLHAAPAEL Sbjct: 128 YRLDNSGAVALVTDASGMEKATLAGELPALRLVFCVESSDLVLPQTTDFWDRLHAAPAEL 187 Query: 188 DPVLTLADDPAMIIYTSGTTGKAKGALHAHRVLLGHLPGVEVSHDSFPQEGDRFWTPADW 247 DPVLTLADDPAMIIYTSGTTGKAKGALHAHRVLLGHLPGVEVSHDSFPQEGDRFWTPADW Sbjct: 188 DPVLTLADDPAMIIYTSGTTGKAKGALHAHRVLLGHLPGVEVSHDSFPQEGDRFWTPADW 247 Query: 248 AWIGGLLDVLLPSLYHGVAVVARRLEKFDAAEVFGLLARHQIRNVFFPPTALKMLRGAAT 307 AWIGGLLDVLLPSLYHGVAVVARRLEKFDAAEVFGLLARHQIRNVFFPPTALKMLRGAAT Sbjct: 248 AWIGGLLDVLLPSLYHGVAVVARRLEKFDAAEVFGLLARHQIRNVFFPPTALKMLRGAAT 307 Query: 308 VRAQADFSLRSVASGGETLGDDLIAWGREALGVTINEFYGQTECNLVVSSSSHCYPSVSG 367 VRAQADFSLRSVASGGETLGDDLIAWGREALGVTINEFYGQTECNLVVSSSSHCYPSVSG Sbjct: 308 VRAQADFSLRSVASGGETLGDDLIAWGREALGVTINEFYGQTECNLVVSSSSHCYPSVSG 367 Query: 368 SMGRAVPGHVVQIVDEQGQVLPHGTVGNIAIRAPDPVMFLRYWRNEEATREKFAGDFLLT 427 SMGRAVPGHVVQIVDEQGQVLPHGTVGNIAIRAPDPVMFLRYWRNEEATREKFAGDFLLT Sbjct: 368 SMGRAVPGHVVQIVDEQGQVLPHGTVGNIAIRAPDPVMFLRYWRNEEATREKFAGDFLLT 427 Query: 428 GDLGSMDEQGYIRYLGRNDDVITSAGYRIGPAPIEECLMRHPAVRIAAVVGVKDALRTEV 487 GDLGSMDEQGYIRYLGRNDDVITSAGYRIGPA IEECLMRHPAVRIAAVVGVKDALRTEV Sbjct: 428 GDLGSMDEQGYIRYLGRNDDVITSAGYRIGPAAIEECLMRHPAVRIAAVVGVKDALRTEV 487 Query: 488 VKAFVVLKDGVTPDEALKAELQQHVRAQLAAHEYPRLISFVAALPTTATGKIMRKTLKEM 547 VKAFVVLKDGVTPDEALKAELQQHVRAQLAAHEYPRLISFVAALPTTATGKIMRKTLKEM Sbjct: 488 VKAFVVLKDGVTPDEALKAELQQHVRAQLAAHEYPRLISFVAALPTTATGKIMRKTLKEM 547 Query: 548 DQ 549 DQ Sbjct: 548 DQ 549 >gi|300310257|ref|YP_003774349.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 266 bits (680), Expect = 3e-72, Method: Compositional matrix adjust. Identities = 191/532 (35%), Positives = 273/532 (51%), Gaps = 25/532 (4%) Query: 27 DIPTHFNIAQAACDRWADGSGRTAIICEDADGSVTSYSYDQLKILSDRFANALRAAGVGR 86 D T N CDR G A+ G YS+ Q++ ++ R AN LR G+ Sbjct: 32 DFETGINACVECCDRHVKGDN-IALEFVSLHGEHQQYSFAQVRSMAARVANLLRQQGIQP 90 Query: 87 GDRIGIYLSQRIETVITHLAAYKLGAITVPLFYLFGPDAIAYRLDNSGAVALVTDASGME 146 G + L + E + T L + GA+ PLF FGP AI +RL SGA +VT+ + + Sbjct: 91 GQIVAGMLPRTPELLATVLGTLRAGAVYQPLFTAFGPKAIEHRLALSGASLIVTNVANRD 150 Query: 147 KATLAGELPALRLVFCVESSDLVLPQTTDFWDRLHAAPAELDPVLTLADDPAMIIYTSGT 206 K P ++ ES+D + DF + A + PVL D +++ TSGT Sbjct: 151 KLDEIANCP--QVCTVRESNDPLRAGDIDFRAAVDAQSDDFAPVLRSGADLMLMMSTSGT 208 Query: 207 TGKAKG------ALHAHRVLLGHLPGVEVSHDSFPQEGDRFWTPADWAWIGGLLDVLLPS 260 TG AKG AL A V + G+ PQ D+FW AD W GL ++ Sbjct: 209 TGAAKGVPVPLSALQAFSVYMREAVGL------LPQ--DKFWNMADPGWAYGLYYAIIGP 260 Query: 261 LYHGVAVVARRLEKFDAAEVFGLLARHQIRNVFFPPTALKMLRGAATVRAQA-DFSLRSV 319 L G + F + + + R I ++ PTA +M+ A A + LR+V Sbjct: 261 LAIGHGITFNE-AAFTVSGTYETIRRLGITSLAGAPTAYRMMMAAGEEAAASVKGRLRAV 319 Query: 320 ASGGETLGDDLIAWGREALGVTINEFYGQTECNLVVSSSSHC-YPSVSGSMGRAVPGHVV 378 +S GE L +++ W AL V I++ YGQTE +VV++ + V GS G A+PG+ V Sbjct: 320 SSAGEPLNAEVVRWFASALDVPIHDHYGQTELGMVVNNHHALRHEVVPGSAGFAMPGYRV 379 Query: 379 QIVDEQGQVLPHGTVGNIAIR-APDPVM-FLRYWRNEEATREKFAGDFLLTGDLGSMDEQ 436 ++DE Q L G +AI A P+ F YW+ + A + TGD ++ Sbjct: 380 VVLDENHQELGANQPGELAIDIARSPLYWFSGYWKQDTPA---IAHGYYSTGDNVELEPN 436 Query: 437 GYIRYLGRNDDVITSAGYRIGPAPIEECLMRHPAVRIAAVVGVKDALRTEVVKAFVVLKD 496 G I ++GR+DDVITSAGYRIGP +E L+ HPAV AV+G+ D RTE+VKAFVVL D Sbjct: 437 GSISFIGRSDDVITSAGYRIGPFDVESALLEHPAVADVAVIGLPDPERTEIVKAFVVLSD 496 Query: 497 GVTPDEALKAELQQHVRAQLAAHEYPRLISFVAALPTTATGKIMRKTLKEMD 548 +AL+ +L QHV+ +L+AH YPR I F+ ALP T +GK+ R L++M+ Sbjct: 497 QFKASDALREQLAQHVKRRLSAHAYPRAIEFLDALPKTPSGKLQRFVLRKME 548 >gi|300310875|ref|YP_003774967.1| acetyl-coenzyme A synthetase [Herbaspirillum seropedicae SmR1] Length = 660 Score = 193 bits (490), Expect = 3e-50, Method: Compositional matrix adjust. Identities = 172/562 (30%), Positives = 263/562 (46%), Gaps = 42/562 (7%) Query: 24 YPWDIPTHFNIAQAACD-RWADGSG-RTAIICEDADGSVTSYSYDQLKILSDRFANALRA 81 Y W N++ D +G+ +TA+I E DG VT SY +L +FAN L++ Sbjct: 73 YKWFADGQVNVSYNCLDVNLENGNADKTAVIFESDDGKVTRVSYRELHQKVCQFANGLKS 132 Query: 82 AGVGRGDRIGIYLSQRIETVITHLAAYKLGAITVPLFYLFGPDAIAYRLDNSGAVALVTD 141 G+ +GDR+ IY+ +E V A ++GA +F F ++ R+ ++GAVA++T Sbjct: 133 LGIKKGDRVVIYMPMSVEGVAAMQACARIGATHSVVFGGFSAKSLHERVIDAGAVAVLTA 192 Query: 142 ------------ASGMEKATLAGELPALR--LVFCVESSDLVLPQTTDFW--DRLHAAPA 185 S +++A G L+ +V+ +++ + D W D + Sbjct: 193 DYQVRGGKQLPLKSIVDEALDMGGCDTLKHVVVYKRAGAEINWVEGRDLWLSDVVANQSD 252 Query: 186 ELDPVLTLADDPAMIIYTSGTTGKAKGALHAHR-VLLGHLPGVEVSHDSFPQEGDRFWTP 244 +P A+ P I+YTSG+TGK KG HA LL + ++ + D P D +W Sbjct: 253 TCEPEWVDAEHPLFILYTSGSTGKPKGVQHASAGYLLWAVLTMKWTFDIKPD--DVYWCT 310 Query: 245 ADWAWIGGLLDVLLPSLYHGVA-VVARRLEKF-DAAEVFGLLARHQIRNVFFPPTALKML 302 AD WI G + L G +V + + +A + ++ARH+ + PTA++ L Sbjct: 311 ADIGWITGHTYIAYGPLAVGATQIVFEGVPTYPNAGRFWDMVARHKATIFYTAPTAIRSL 370 Query: 303 RGAATVR-----AQADF-SLRSVASGGETLGDDLIAWGREALG---VTINEFYGQTEC-N 352 AA Q D SLR + S GE + + W + +G I + + QTE Sbjct: 371 IKAADADEKVHPKQYDLSSLRILGSVGEPINPEAWMWYYKNVGHENCPIVDTFWQTETGG 430 Query: 353 LVVSSSSHCYPSVSGSMGRAVPGHVVQIVDEQGQVLPHGTVGNIAIRAPDPVMFLRYWRN 412 ++S P V GS +PG IVDE G +P+G G + ++ P P M W + Sbjct: 431 HMISPLPGATPQVPGSCTLPLPGITAAIVDETGNDIPNGNGGILVVKRPWPSMIRTIWND 490 Query: 413 EEATR-----EKFAGDFLLTGDLGSMD-EQGYIRYLGRNDDVITSAGYRIGPAPIEECLM 466 E + E+ G L GD + E GY GR DDV+ +G+R+G IE L+ Sbjct: 491 PERFKKSYFPEELGGKIYLAGDGAVRNKETGYFTITGRIDDVLNVSGHRMGTMEIESALV 550 Query: 467 RHPAVRIAAVVGVKDALRTEVVKAFVVLKD-GVTPDEA--LKAELQQHVRAQLAAHEYPR 523 + V AAVVG D E + AFVVLK T DEA + EL+ V ++ P+ Sbjct: 551 ANSIVAEAAVVGKPDETTGESICAFVVLKRPRPTGDEAKQIAKELRDWVAKEIGPIAKPK 610 Query: 524 LISFVAALPTTATGKIMRKTLK 545 I F LP T +GKIMR+ L+ Sbjct: 611 EIRFGDNLPKTRSGKIMRRLLR 632 >gi|300312417|ref|YP_003776509.1| propionyl-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 628 Score = 168 bits (425), Expect = 1e-42, Method: Compositional matrix adjust. Identities = 164/572 (28%), Positives = 267/572 (46%), Gaps = 65/572 (11%) Query: 26 WDIPTHFNIAQAACDRW----ADGSGRTAIICEDADGSVTSYSYDQLKILSDRFANALRA 81 W I N+ A DR A + AI E V +Y+ Q ++++ A +++A Sbjct: 45 WFIDGQTNLCHNAVDRHLPERAQQAALIAISTETNSERVYTYAELQQEVMA--MAASMQA 102 Query: 82 AGVGRGDRIGIYLSQRIETVITHLAAYKLGAITVPLFYLFGPDAIAYRLDNSGAVALVTD 141 GV RGDR+ IY+ E V LA ++GA+ +F F +++A R+D++ +V+ Sbjct: 103 LGVQRGDRVLIYMPMIAEAVFAMLACARIGAVHSVVFGGFASNSLASRIDDAQPRLIVSA 162 Query: 142 ASGMEKATLAGELPALRLVFCVESSDLVLPQTTDFWDR-----LHAAPAELD-------- 188 +G + +P L+ + PQ DR A ++D Sbjct: 163 DAGSRGGKV---IPYKGLLDEAIAMAQHRPQHVLLVDRGLAPMARTAQRDVDYAPLRTQH 219 Query: 189 -----PVLTLADD-PAMIIYTSGTTGKAKGALHAHRVLLGHLPGVEVS--HDSFPQEGDR 240 PV L + + I+YTSGTTGK KG R + G+ + S H Q G+ Sbjct: 220 LGQQVPVTWLESNASSYILYTSGTTGKPKGV---QRDVGGYAVALASSMQHIFCGQPGET 276 Query: 241 FWTPADWAWIGGLLDVLLPSLYHGVAVVARR--LEKFDAAEVFGLLARHQIRNVFFPPTA 298 ++ +D W+ G ++ L G+A V + DA + ++ ++++ +F PTA Sbjct: 277 YFCTSDIGWVVGHSYIVYGPLIAGMATVLYEGLPIRPDAGIWWSIVEKYKVTRMFSAPTA 336 Query: 299 LKMLRGA--ATVRAQADFSLRSVASGGETLGDDLIAWGREALGVTINEFYGQTECNLVVS 356 +++L+ + SL+++ GE L + +W AL V + + Y QTE + Sbjct: 337 IRVLKKQPPECMERYDTSSLKALYLAGEPLDETTSSWISGALKVPVIDNYWQTESGWPII 396 Query: 357 SSSHCY---PSVSGSMGRAVPGHVVQIVDE-QGQVLPHGTVGNIAIRAPDPVMFLR---- 408 S + P+ GS G +PG+ + I+DE G+ G +AI P P ++ Sbjct: 397 SIAKGIDDKPTRLGSPGVPMPGYRLAILDEATGEPCGPDQKGVVAIEGPLPPGCMQTVYG 456 Query: 409 --------YWRNEEATREKFAGDFLLTGDLGSMDEQGYIRYLGRNDDVITSAGYRIGPAP 460 YWR+ RE ++ T D G D+ GY LGR DDVI AG+R+G Sbjct: 457 DDERFVNTYWRS--LPREVYS-----TFDWGIRDKDGYYFILGRTDDVINVAGHRLGTRE 509 Query: 461 IEECLMRHPAVRIAAVVGVKDALRTEVVKAFVVLKDG---VTPDE--ALKAELQQHVRAQ 515 IEE + HP V AVVGV+D L+ +V AF + K TP++ AL+AE+ V Q Sbjct: 510 IEESISSHPNVSEVAVVGVEDKLKGQVAFAFAIPKQAYTIATPEQRKALEAEIMAVVDKQ 569 Query: 516 LAAHEYPRLISFVAALPTTATGKIMRKTLKEM 547 + A P + FV+ LP T +GK++R+ ++ + Sbjct: 570 IGAVGRPARVFFVSGLPKTRSGKLLRRAIQSI 601 >gi|300313115|ref|YP_003777207.1| long-chain-fatty-acid-CoA ligase [Herbaspirillum seropedicae SmR1] Length = 556 Score = 155 bits (391), Expect = 1e-38, Method: Compositional matrix adjust. Identities = 141/510 (27%), Positives = 228/510 (44%), Gaps = 36/510 (7%) Query: 56 ADGSVTSYSYDQLKILSDRFANALRAAGVGRGDRIGIYLSQRIETVITHLAAYKLGAITV 115 ADGS + ++ D A+AL GV +GD++ ++ + + AA+K+G + V Sbjct: 56 ADGSDRLIPWREINARVDALAHALTRRGVKKGDKMLVHSRNNQQIFESAWAAFKMGVVWV 115 Query: 116 PLFYLFGPDAIAYRLDNSGAVALVTDASGMEKA-TLAGELPALRLVFCV---ESSDLVLP 171 P + P AY +SGA ++ D + + PAL V + + +L Sbjct: 116 PTNFRITPPEAAYLGQSSGACVMLYDRGFAQYVDAVKAASPALEHVVALADARAGELDYE 175 Query: 172 QTTDFWDRLHAAPAELDPVLTLADDPAMIIYTSGTTGKAKGALHAHRVLLGHLPGVEVSH 231 AE+D DDP YTSGTTG K + +H G + V +H Sbjct: 176 ALVREGSGAAFREAEVD-----YDDPLWFFYTSGTTGHPKAGMLSH----GQMAFVVTNH 226 Query: 232 --DSFP--QEGDRFWTPADWAWIGGLLDVLLPSLYHGVAVVARRLEKFDAAEVFGLLARH 287 D P R A + G+ V+ + G A + E+ E + L+ ++ Sbjct: 227 LADLMPGLTHQSRSLVVAPLSHGAGVHAVI--NTARGAASILLSSERLVVEEAWQLVEKY 284 Query: 288 QIRNVFFPPTALKMLRGAATVRAQADFSLRSVASGGETLGDDLIAWGREALGVTINEFYG 347 ++ N+F PT +K+L +V SL+ V G + + + LG + ++YG Sbjct: 285 RVDNLFTVPTIVKILVEDESVDRYDHSSLKHVIYAGAPMYRADQVYALKKLGKVLVQYYG 344 Query: 348 QTECN---------LVVSSSSHCYPSVSGSMGRAVPGHVVQIVDEQGQVLPHGTVGNIAI 398 E L +H V G+ G G + I+++ GQ L G I + Sbjct: 345 LGEVTGNITFLPPYLHFEDDAHPQARV-GTCGMPRTGMQIAILNDDGQELAPFETGEICV 403 Query: 399 RAPDPVMFLRYWRNEEATREKFAGDFLLTGDLGSMDEQGYIRYLGRNDDVITSAGYRIGP 458 R P +F+ Y N EA + F D+ TGDLG +D+ G++ GR+ D+ S G + P Sbjct: 404 RGP--AVFMGYHNNPEANAKAFKHDWFHTGDLGHVDQDGFLYITGRSSDMYISGGSNVYP 461 Query: 459 APIEECLMRHPAVRIAAVVGVKDALRTEVVKAFVVLKDGVTPD-EALKAELQQHVRAQLA 517 IEE L+ HPAV AV+GV D E A +V K G D EAL A H+ +++ Sbjct: 462 REIEEALLTHPAVSEVAVLGVPDPKWGESGLAVIVCKAGQQVDGEALLA----HLESRIG 517 Query: 518 AHEYPRLISFVAALPTTATGKIMRKTLKEM 547 +++PR + ++P + GKI++K +K + Sbjct: 518 KYKWPRRFIYWDSMPKSGYGKIVKKQIKAL 547 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,860,628 Number of extensions: 83167 Number of successful extensions: 343 Number of sequences better than 1.0e-08: 12 Number of HSP's gapped: 289 Number of HSP's successfully gapped: 17 Length of query: 549 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 459 Effective length of database: 1,193,720 Effective search space: 547917480 Effective search space used: 547917480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)