BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0005.p (467 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309362|ref|YP_003773454.1| transmembrane sensory transduct... 933 0.0 gi|300312357|ref|YP_003776449.1| cobalt/zinc/cadmium resistance ... 291 6e-80 gi|300311800|ref|YP_003775892.1| two component heavy metal senso... 213 3e-56 gi|300312679|ref|YP_003776771.1| periplasmic sensor signal trans... 136 3e-33 gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine ... 129 4e-31 >gi|300309362|ref|YP_003773454.1| transmembrane sensory transduction histidine kinase for cobalt/zinc/ cadmium resistance transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 467 Score = 933 bits (2411), Expect = 0.0, Method: Compositional matrix adjust. Identities = 467/467 (100%), Positives = 467/467 (100%) Query: 1 MRRFAPASLSLRLVALFGLLAMVILAALGTYLSHSLHVQLERRDDEELAGKCRSINHLVE 60 MRRFAPASLSLRLVALFGLLAMVILAALGTYLSHSLHVQLERRDDEELAGKCRSINHLVE Sbjct: 1 MRRFAPASLSLRLVALFGLLAMVILAALGTYLSHSLHVQLERRDDEELAGKCRSINHLVE 60 Query: 61 HAASLEDILARRHLVLDTLTGHDQLLVRIARADGSTLLESHDPTRTPPGIDLSQVDIRAW 120 HAASLEDILARRHLVLDTLTGHDQLLVRIARADGSTLLESHDPTRTPPGIDLSQVDIRAW Sbjct: 61 HAASLEDILARRHLVLDTLTGHDQLLVRIARADGSTLLESHDPTRTPPGIDLSQVDIRAW 120 Query: 121 DSIQSWKLGDLRARYVASKPVTRSGEQAVVLVVRTASDRMLLLAEYRSDVWWAAGVGALL 180 DSIQSWKLGDLRARYVASKPVTRSGEQAVVLVVRTASDRMLLLAEYRSDVWWAAGVGALL Sbjct: 121 DSIQSWKLGDLRARYVASKPVTRSGEQAVVLVVRTASDRMLLLAEYRSDVWWAAGVGALL 180 Query: 181 AILMSYFLVRSGLRPLRTMAAQTRAVSANRLDTRLDLQAAPRELHEIVESFNEMLDRLHR 240 AILMSYFLVRSGLRPLRTMAAQTRAVSANRLDTRLDLQAAPRELHEIVESFNEMLDRLHR Sbjct: 181 AILMSYFLVRSGLRPLRTMAAQTRAVSANRLDTRLDLQAAPRELHEIVESFNEMLDRLHR 240 Query: 241 SFQQLSQFSADLAHDVRTPLNNLMVQTQVALGKPRGIDEYQALLSSNIEEYERLSRMMES 300 SFQQLSQFSADLAHDVRTPLNNLMVQTQVALGKPRGIDEYQALLSSNIEEYERLSRMMES Sbjct: 241 SFQQLSQFSADLAHDVRTPLNNLMVQTQVALGKPRGIDEYQALLSSNIEEYERLSRMMES 300 Query: 301 MLFLARAENAHVVLQWQELDVAQELTRIADYFEGIADEAQVRLVVQGAGRLQADATLLRR 360 MLFLARAENAHVVLQWQELDVAQELTRIADYFEGIADEAQVRLVVQGAGRLQADATLLRR Sbjct: 301 MLFLARAENAHVVLQWQELDVAQELTRIADYFEGIADEAQVRLVVQGAGRLQADATLLRR 360 Query: 361 AVGNLVANAIRYTPADGVIRLQVDAAGDGMEISVSNPGAGIPAEQLERLFDRFYRADPSR 420 AVGNLVANAIRYTPADGVIRLQVDAAGDGMEISVSNPGAGIPAEQLERLFDRFYRADPSR Sbjct: 361 AVGNLVANAIRYTPADGVIRLQVDAAGDGMEISVSNPGAGIPAEQLERLFDRFYRADPSR 420 Query: 421 SSAGGSSGLGLAIVRSIMMLHGGQASVTSSPGQLTVFSLRFPGRSAV 467 SSAGGSSGLGLAIVRSIMMLHGGQASVTSSPGQLTVFSLRFPGRSAV Sbjct: 421 SSAGGSSGLGLAIVRSIMMLHGGQASVTSSPGQLTVFSLRFPGRSAV 467 >gi|300312357|ref|YP_003776449.1| cobalt/zinc/cadmium resistance two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 291 bits (746), Expect = 6e-80, Method: Compositional matrix adjust. Identities = 184/470 (39%), Positives = 279/470 (59%), Gaps = 18/470 (3%) Query: 8 SLSLRLVALFGLLAMVILAALGTYLSHSLHVQLERRDDEELAGKCRSINHLVEHAASLED 67 +L+ R+ ALF L+ +++ LG YL S L +R D+ L G+ +L+ ++E Sbjct: 12 TLTTRVTALFALMTSLVVTGLGFYLDRSAGQALSQRADQALIGRVEHFRNLMHDLYNVEQ 71 Query: 68 ILARRHLVLDTLTGHDQLLVRIARADGSTLLESHDP--------TRTPPGIDLSQVDIRA 119 + +R + +++ G++Q VRI R G +P T P G +S +R Sbjct: 72 -MEQRPALFESMMGNEQD-VRIFRRQGEAPFIQSNPDHLVPPAMTPLPVGSPVSSASLRT 129 Query: 120 WDSIQSWKLGDLRARYVAS-KPVTRSGEQAVVLVVRTASDRMLLLAEYRSDVWWAAGVGA 178 S + +R R+V++ V + G+ ++ ++ YR + AA Sbjct: 130 -----SQRADGVRVRWVSALADVGKGGDTVEIVAAYVMVQEARMMRSYRWRIAGAAAAAV 184 Query: 179 LLAILMSYFLVRSGLRPLRTMAAQTRAVSANRLDTRLDLQAAPRELHEIVESFNEMLDRL 238 +L L+ + L+R GL+PL M+ + ++ + L RLD + P EL + SFN MLDRL Sbjct: 185 VLTALLGFLLLRRGLQPLEAMSQRAAQITPDHLSARLDQEGMPAELRRVALSFNAMLDRL 244 Query: 239 HRSFQQLSQFSADLAHDVRTPLNNLMVQTQVALGKPRGIDEYQALLSSNIEEYERLSRMM 298 + L+QFS DLAH++RTP+N LM Q+QVALG+ R +EY+ +L SN+EE +RLSR++ Sbjct: 245 EAGYAHLAQFSGDLAHEIRTPINVLMGQSQVALGQRRSPEEYEQVLESNVEELQRLSRIV 304 Query: 299 ESMLFLARAENAHVVLQWQELDVAQELTRIADYFEGIADEAQVRLVVQGAGRLQADATLL 358 E++LFLA+A++A + ++ LD+A+EL RI DYFEGIADE +R VQ AG+ QA+ + Sbjct: 305 ENILFLAQADHAALAVERNALDLAEELGRITDYFEGIADERGMRFDVQAAGQCQANLIMW 364 Query: 359 RRAVGNLVANAIRYTPADGVIRLQVDAAGDGMEISVSNPGAGIPAEQLERLFDRFYRADP 418 RR V NLV NA+RY A+ IR+ ++ G+G I V N + A+ + R+FDRFYR D Sbjct: 365 RRVVSNLVINAVRYGRAESTIRILAESDGEGSRIMVENRSEDVTAQAMARMFDRFYRGDR 424 Query: 419 SRSSAGGSSGLGLAIVRSIMMLHGGQASV-TSSPGQLTVFSLRFPGRSAV 467 SRS+ S+GLGLAIV++IM LHGG A+V +PG + FSLRFP +AV Sbjct: 425 SRSAFTESNGLGLAIVKAIMALHGGTAAVECPAPGWIR-FSLRFPSAAAV 473 >gi|300311800|ref|YP_003775892.1| two component heavy metal sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 467 Score = 213 bits (542), Expect = 3e-56, Method: Compositional matrix adjust. Identities = 109/281 (38%), Positives = 177/281 (62%), Gaps = 4/281 (1%) Query: 176 VGALLAILMSYFLVRSGLRPLRTMAAQTRAVSANRLDTRLDLQAAPRELHEIVESFNEML 235 +G +LA++ SY++ R+GL PLR M+ + ++ S +L R+ ++A P+EL + +FN L Sbjct: 176 LGIVLAVISSYWIARAGLAPLRQMSDRAQSFSPRKLSARMPVEAMPQELSVLALAFNGAL 235 Query: 236 DRLHRSFQQLSQFSADLAHDVRTPLNNLMVQTQVALGKPRGIDEYQALLSSNIEEYERLS 295 DR+ ++ QL F+AD+AH++RTPL NL+ +TQVAL + R E++ +L SN+EE +R+ Sbjct: 236 DRIEDAYTQLEAFNADVAHELRTPLANLIGETQVALARERSAGEFKDVLQSNLEELDRIR 295 Query: 296 RMMESMLFLARAENAHVVLQWQELDVAQELTRIADYFEGIADEAQVRLVVQGAGRLQA-- 353 ++ MLFLAR++ VA E+ + D+FE + D++ +R+ V G A Sbjct: 296 SIVNDMLFLARSDQGEAATARVPARVADEVQKTVDFFEFVLDDSGMRIEVAGDTEATAMM 355 Query: 354 DATLLRRAVGNLVANAIRYTPADGVIRLQVDAAGDGMEISVSNPGAGIPAEQLERLFDRF 413 + L +RA+ NL+ NAI+++P ++R+ + + + ++VSNPG I L RLFDRF Sbjct: 356 ETALFKRALTNLIQNAIQHSPEGALLRIDIACQEETVRVAVSNPGKPIDEHHLPRLFDRF 415 Query: 414 YRADPSRSSAGGS--SGLGLAIVRSIMMLHGGQASVTSSPG 452 YR D +R GS GLGLAIV+++ +HGG+A +S G Sbjct: 416 YRVDSARQDINGSHGHGLGLAIVKAVARMHGGRAFASSKEG 456 >gi|300312679|ref|YP_003776771.1| periplasmic sensor signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 136 bits (343), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 96/291 (32%), Positives = 148/291 (50%), Gaps = 8/291 (2%) Query: 175 GVGALLAILMSYFLVRSGLRPLRTMAAQTRAVSANRLDTRLDLQAAPRELHEIVESFNEM 234 G +I + VR GLRPL+++ +L +D + AP EL ++ + NE+ Sbjct: 168 GALTFASIFVVLVAVRRGLRPLKSLQRNLEQRDHGKLGA-IDGRGAPVELQPLIVAMNEL 226 Query: 235 LDRLHRSFQQLSQFSADLAHDVRTPLNNLMVQTQVALGKPRGIDEYQALLSSNIEEYERL 294 + R+++ Q F AD+AH +RTPL L +Q ++ K + + L+ ER+ Sbjct: 227 MARINKGEQAQQNFMADVAHQLRTPLTGLKLQLELLHDKHQDQPDTARSLAMMNSSVERM 286 Query: 295 SRMMESMLFLARAENA-HVVLQWQELDVAQELTRIADYFEGIADEAQVRLVVQ-GAGRLQ 352 R +L LAR+E + +EL + + + +F AD+ ++ L RL+ Sbjct: 287 IRQSRQLLALARSEPGLFESRKLEELSLDKLVEESVQHFIEEADKKRIDLGFDLRPARLR 346 Query: 353 ADATLLRRAVGNLVANAIRYTPADGVIRLQVDAAGDGMEISVSNPGAGIPAEQLERLFDR 412 D LL + NL+ NA+RY+P G + ++ D +SV + G GI E E +FDR Sbjct: 347 GDRFLLSDLIDNLIDNAVRYSPPHGTVTVRCLEEQDATVLSVEDSGPGIAPEHRELIFDR 406 Query: 413 FYRADPSRSSAGGSSGLGLAIVRSIMMLHGGQASV-TSSPGQLTVFSLRFP 462 FYRA+ + SGLGLAIVR I HGG SV GQ T+F++RFP Sbjct: 407 FYRANDKVA----GSGLGLAIVREIAHDHGGVISVDCKEQGQGTIFTVRFP 453 >gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 444 Score = 129 bits (325), Expect = 4e-31, Method: Compositional matrix adjust. Identities = 94/272 (34%), Positives = 141/272 (51%), Gaps = 19/272 (6%) Query: 183 LMSYFLVRSGLRPLRTMAAQTRAVSANRLDTRLDLQAAPRELHEIVESFNEMLDRLHRSF 242 ++S+ V GL P+R AA+ A N L D+ P+E+ + + N++L RL + Sbjct: 155 VLSWVAVSRGLAPVRKAAAEVAARDMNSLQPLADV-GMPQEIQPLTHALNDLLARLKHAT 213 Query: 243 QQLSQFSADLAHDVRTPLNNLMVQTQVALGKPRGIDEYQ--ALLSSNIEEYERLSRMMES 300 + F AD AH++RTPL L +QTQ+A R +DE + A + ER + ++ Sbjct: 214 ETQRAFVADAAHELRTPLTALRLQTQLA---ERAVDEEERRAAFADLKNGLERTTHLVHQ 270 Query: 301 MLFLARAENAHVVLQWQELDVAQELTRIADYFEGIADEAQVRLVVQGAG------RLQAD 354 +L LAR E + + ++D+ L + +A+ + L + G R+ + Sbjct: 271 LLTLARQEPD--AMSFTQVDLRALLGSVVGDLSSVAEHGGIDLGLSDQGDDGRPLRVTGN 328 Query: 355 ATLLRRAVGNLVANAIRYTPADGVIRLQVDAAGDG-MEISVSNPGAGIPAEQLERLFDRF 413 A LR NLV NA+RYTPA GV+ + VD + G + V + G GI +L R+FDRF Sbjct: 329 ADALRILFNNLVDNALRYTPAGGVVDVGVDLSTAGYCTVVVQDSGPGIAESELPRVFDRF 388 Query: 414 YRADPSRSSAGG----SSGLGLAIVRSIMMLH 441 YRA S+ +G SGLGLAIVR I LH Sbjct: 389 YRAQHSQEGSGSRAAFGSGLGLAIVRQIADLH 420 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.134 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,389,790 Number of extensions: 56395 Number of successful extensions: 390 Number of sequences better than 1.0e-08: 41 Number of HSP's gapped: 334 Number of HSP's successfully gapped: 41 Length of query: 467 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 378 Effective length of database: 1,198,455 Effective search space: 453015990 Effective search space used: 453015990 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)