BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0034.0002.p (778 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309360|ref|YP_003773452.1| hypothetical protein Hsero_0014... 1480 0.0 gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [... 337 2e-93 gi|300314042|ref|YP_003778134.1| signal transduction protein con... 278 2e-75 gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protei... 276 5e-75 gi|300312026|ref|YP_003776118.1| signal transduction protein con... 273 5e-74 >gi|300309360|ref|YP_003773452.1| hypothetical protein Hsero_0014 [Herbaspirillum seropedicae SmR1] Length = 726 Score = 1480 bits (3831), Expect = 0.0, Method: Compositional matrix adjust. Identities = 722/726 (99%), Positives = 724/726 (99%) Query: 53 MLRHGLVLPVWNLDQSDIMALLQGAMLDRQVVSIEVIDPEEKTLARRAEPQRSAGHLIHR 112 MLRHGLVLPVWNLDQSDIMALL+GAMLDRQVVSIEVIDPEEK LARRAEPQRSAGHLIHR Sbjct: 1 MLRHGLVLPVWNLDQSDIMALLKGAMLDRQVVSIEVIDPEEKALARRAEPQRSAGHLIHR 60 Query: 113 TAPLVMQQNQQDVSLGSLHIVVSDHQLQKKLAADKSFYALVLLAQAIVSLLIIGLLVYRR 172 TAPLVMQQNQQDVSLGSLHIVVSDHQLQKKLAADKSFYALVLLAQAIVSLLIIGLLVYRR Sbjct: 61 TAPLVMQQNQQDVSLGSLHIVVSDHQLQKKLAADKSFYALVLLAQAIVSLLIIGLLVYRR 120 Query: 173 ILGPIGKLTQATARIQRGSFEQGIDLPQQDEIGQLARHMEAMQASLKTLFDEQAAILGNI 232 ILGPIGKLTQATARIQRGSFEQGIDLPQQDEIGQLARHMEAMQASLKTLFDEQAAILGNI Sbjct: 121 ILGPIGKLTQATARIQRGSFEQGIDLPQQDEIGQLARHMEAMQASLKTLFDEQAAILGNI 180 Query: 233 PAGVLFVRKGKIVFGNEAAQALLARDDRRLVGVAAEDLFVESVQQAVYVARAVARSSGAA 292 PAGVLFVRKGKIVFGNEAAQALLARDDRRLVGVAAEDLFVESVQQAVYVARAVARSSGAA Sbjct: 181 PAGVLFVRKGKIVFGNEAAQALLARDDRRLVGVAAEDLFVESVQQAVYVARAVARSSGAA 240 Query: 293 GEIVLLRNASGASFPAELHTSLIDPARPGEQIWVVIDVSARLEAENRIDQLAFYDPVTQL 352 GEIVLLRNASGASFPAELHTSLIDPARPGEQIWVVIDVSARLEAENRIDQLAFYDPVTQL Sbjct: 241 GEIVLLRNASGASFPAELHTSLIDPARPGEQIWVVIDVSARLEAENRIDQLAFYDPVTQL 300 Query: 353 PNKKLFMDRLRRGLDRRRTSGHAGAVFVMEIHRADLSYGSVKVDEMEQLLIECARRFSSC 412 PNKKLFMDRLRRGLDRRRTSGHAGAVFVMEIHRADLSYGSVK DEMEQLLIECARRFSSC Sbjct: 301 PNKKLFMDRLRRGLDRRRTSGHAGAVFVMEIHRADLSYGSVKADEMEQLLIECARRFSSC 360 Query: 413 ITAEQTVARMEGEKFALGTEIRSNELAECIRYCEALAQRILDAFTDPQSLSAARFPCSVN 472 ITAEQTVARMEGEKFALGTEIRSNELAECIRYCEALAQRILDAFTDPQSLSAARFPCSVN Sbjct: 361 ITAEQTVARMEGEKFALGTEIRSNELAECIRYCEALAQRILDAFTDPQSLSAARFPCSVN 420 Query: 473 LGINIVRDEQYGASELVMQAELAMVQSRAAGRNVFRFFDPAMQALANQRSQLEAELRQAV 532 LGINIVRDEQYGASELVMQAELAMVQSRAAGRNVFRFFDPAMQALANQRSQLEAELRQAV Sbjct: 421 LGINIVRDEQYGASELVMQAELAMVQSRAAGRNVFRFFDPAMQALANQRSQLEAELRQAV 480 Query: 533 ARRQFIVYYQPQVTYEGDVVGAEALVRWQHPIRGIVSPTTFISLAEDMGLMDEIGRQVLH 592 ARRQFIVYYQPQVTYEGDVVGAEALVRWQHPIRGIVSPTTFISLAEDMGLMDEIGRQVLH Sbjct: 481 ARRQFIVYYQPQVTYEGDVVGAEALVRWQHPIRGIVSPTTFISLAEDMGLMDEIGRQVLH 540 Query: 593 RICSDLENWQREGLARDLVVAINVSAQEFKVDNFVYRFREIMREHHVPSHSVKVELTESM 652 RIC+DLENWQREGLARDLVVAINVSAQEFKVDNFVYRFREIMREHHVPSHSVKVELTESM Sbjct: 541 RICTDLENWQREGLARDLVVAINVSAQEFKVDNFVYRFREIMREHHVPSHSVKVELTESM 600 Query: 653 MVDHIEDVITKMQALRSNEVSISLDDFGTGYSSLSYLGRFPIDQIKIDQTFVREMQNDAA 712 MVDHIEDVITKMQALRSNEVSISLDDFGTGYSSLSYLGRFPIDQIKIDQTFVREMQNDAA Sbjct: 601 MVDHIEDVITKMQALRSNEVSISLDDFGTGYSSLSYLGRFPIDQIKIDQTFVREMQNDAA 660 Query: 713 MASIIRSIIMLGQSLNLSIIAEGVETEQQRDFLHAQGCLLYQGYWYSKPIPGEQFADLLN 772 MASIIRSIIMLGQSLNLSIIAEGVETEQQRDFLHAQGCLLYQGYWYSKPIPGEQFADLLN Sbjct: 661 MASIIRSIIMLGQSLNLSIIAEGVETEQQRDFLHAQGCLLYQGYWYSKPIPGEQFADLLN 720 Query: 773 TPGRFR 778 TPGRFR Sbjct: 721 TPGRFR 726 >gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1036 Score = 337 bits (864), Expect = 2e-93, Method: Compositional matrix adjust. Identities = 183/440 (41%), Positives = 266/440 (60%), Gaps = 2/440 (0%) Query: 329 DVSARLEAENRIDQLAFYDPVTQLPNKKLFMDRLRRGLDRR-RTSGHAGAVFVMEIHRAD 387 D+S R AE RI +LAF+D +T+LPN+ L +DRL++ + RT A +F+ H Sbjct: 584 DISDRKLAEERIRELAFFDALTRLPNRTLLLDRLKQAIALSLRTKTCAALLFIDLDHFKT 643 Query: 388 LSYGSVKVDEMEQLLIECARRFSSCITAEQTVARMEGEKFALGTEIRSNELAECIRYCEA 447 L+ ++ ++ + LL + A+R + + TVAR+ G++F + E + E A Sbjct: 644 LN-DTIGHEKGDTLLKQVAQRLLANVRENDTVARVGGDEFVVVLEGLNELQQEAATQTRA 702 Query: 448 LAQRILDAFTDPQSLSAARFPCSVNLGINIVRDEQYGASELVMQAELAMVQSRAAGRNVF 507 + ++IL A L + + ++G + EL+ QA+LAM +++ GRN Sbjct: 703 IGEKILTALGSTYQLDDVEYRTTASIGATVFLGRAASVDELMKQADLAMYKAKETGRNAL 762 Query: 508 RFFDPAMQALANQRSQLEAELRQAVARRQFIVYYQPQVTYEGDVVGAEALVRWQHPIRGI 567 RFFDPAMQ +R+ LEA LR+A+ Q +++YQPQV G V GAE LVRWQHP RG+ Sbjct: 763 RFFDPAMQTAVLERATLEAGLRKAIDGNQLLLHYQPQVVEGGRVTGAEVLVRWQHPQRGM 822 Query: 568 VSPTTFISLAEDMGLMDEIGRQVLHRICSDLENWQREGLARDLVVAINVSAQEFKVDNFV 627 V P FI LAE+ GL+ +G VL C L W + DL +A+NVSA + + +FV Sbjct: 823 VPPGDFIPLAEETGLILSLGNWVLETACRQLATWAAQPQMADLSIAVNVSAPQLREPDFV 882 Query: 628 YRFREIMREHHVPSHSVKVELTESMMVDHIEDVITKMQALRSNEVSISLDDFGTGYSSLS 687 + + +K+ELTES++VD++ED+I KM AL++ V SLDDFGTGYSSLS Sbjct: 883 QTVLDTIERTGANPRRLKLELTESLLVDNVEDIIQKMFALKAYGVEFSLDDFGTGYSSLS 942 Query: 688 YLGRFPIDQIKIDQTFVREMQNDAAMASIIRSIIMLGQSLNLSIIAEGVETEQQRDFLHA 747 YL R P++Q+KIDQ+FVR++ D ASI ++I+ L QSL L +IAEGVET+ QR FL Sbjct: 943 YLKRLPLNQLKIDQSFVRDVLIDPNDASIAKTIVNLAQSLGLGVIAEGVETDAQRSFLAD 1002 Query: 748 QGCLLYQGYWYSKPIPGEQF 767 GC YQGY++ +P+ E F Sbjct: 1003 AGCHAYQGYFFCRPVTVEAF 1022 >gi|300314042|ref|YP_003778134.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 948 Score = 278 bits (710), Expect = 2e-75, Method: Compositional matrix adjust. Identities = 158/444 (35%), Positives = 251/444 (56%), Gaps = 7/444 (1%) Query: 329 DVSARLEAENRIDQLAFYDPVTQLPNKKLFMDRLRRGLDRRRTSGHAGAVFVMEIHRADL 388 D+++ E ++ A D +T L N+ + L + + A V +++ R Sbjct: 504 DITSMKRYEAELEFQASRDTLTGLINRHMLRHHLEQAIGVAERQKQALWVVFVDLDRFKF 563 Query: 389 SYGSVKVDEMEQLLIECARRFSSCITAEQTVARMEGEKFALGTEIRSNELAECIRYCEAL 448 ++ + LL A R + + TVAR+ G++F + +EL R Sbjct: 564 VNDTLGHKAGDTLLRTVAGRLRAAVREADTVARLGGDEFIMILP-EQDELTLDTRSL--- 619 Query: 449 AQRILDAFTDPQSLSAARFPCSVNLGINIVRDEQYGASELVMQAELAMVQSRAAGRNVFR 508 QRI+DA P +L + ++G+ ++ L+ A++AM + + GRN F+ Sbjct: 620 -QRIMDAVAAPVTLGGHTLFMTCSVGVACYPNDGTDGETLIKHADIAMYRGKQTGRNNFQ 678 Query: 509 FFDPAMQALANQRSQLEAELRQAVARRQFIVYYQPQVTY-EGDVVGAEALVRWQHPIRGI 567 F+ PAM A +R ++E +LR A+ +F+++YQP+V G V+G+EAL+RWQHP G+ Sbjct: 679 FYTPAMNEQALERLRIEGDLRNALDHGEFLLHYQPKVDLASGRVIGSEALIRWQHPQLGM 738 Query: 568 VSPTTFISLAEDMGLMDEIGRQVLHRICSDLENWQREGLARDLVVAINVSAQEFKVDNFV 627 VSP FI LAE+ GL+ IG VL C+ WQ G A D+ V++N+S +F + V Sbjct: 739 VSPARFIGLAEETGLILPIGAWVLRAACTQCRQWQLGGYA-DISVSVNLSVGQFLQKDLV 797 Query: 628 YRFREIMREHHVPSHSVKVELTESMMVDHIEDVITKMQALRSNEVSISLDDFGTGYSSLS 687 +++ + +P S+++ELTES+M+ +E I + L+ V +S+DDFGTGYSSL+ Sbjct: 798 SSIADVLEDTGLPPESLELELTESLMMTDVEHAIGILAGLKKLGVRLSIDDFGTGYSSLA 857 Query: 688 YLGRFPIDQIKIDQTFVREMQNDAAMASIIRSIIMLGQSLNLSIIAEGVETEQQRDFLHA 747 YL RFPID +KID++FVR++ DA A+I SII L + L L +IAEGVET++Q D+L Sbjct: 858 YLKRFPIDVLKIDRSFVRDITIDADDAAITASIISLARHLKLRVIAEGVETDEQLDYLRH 917 Query: 748 QGCLLYQGYWYSKPIPGEQFADLL 771 GC QGY +S P+P ++F +L Sbjct: 918 YGCDEIQGYLFSPPVPADKFEQIL 941 >gi|300312606|ref|YP_003776698.1| EAL domain transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 695 Score = 276 bits (705), Expect = 5e-75, Method: Compositional matrix adjust. Identities = 158/434 (36%), Positives = 253/434 (58%), Gaps = 10/434 (2%) Query: 336 AENRIDQLAFYDPVTQLPNKKLFMDRLRRGLDRR-RTSGHAGAVFVMEIHRADLSYGSVK 394 A + Q+ +D +T+LPN+ L DRL + +++ R GH +F M++ S+ Sbjct: 258 ANEELTQMVLHDQLTRLPNRTLLEDRLNQAINKAAREQGHFALMF-MDLDGFKAINDSLG 316 Query: 395 VDEMEQLLIECARRFSSCITAEQTVARMEGEKFALGTEIRSNELAECIRYCEALAQRILD 454 ++LL+E A+R +S A TVAR+ G++F + ++ + E A + A+++++ Sbjct: 317 HHVGDRLLVEVAQRLTSSSRANDTVARLGGDEFVILVDLNTPEDAMGV------AEKLVE 370 Query: 455 AFTDPQSLSAARFPCSVNLGINIVRDEQYGASELVMQAELAMVQSRAAGRNVFRFFDPAM 514 A P + S ++GI I ++ +LV+ A+ AM ++ +GRN + FF+P+M Sbjct: 371 AVNQPFKVDQHELRVSASVGIAIYPEDGATRHDLVINADAAMYHTKRSGRNGYHFFEPSM 430 Query: 515 QALANQRSQLEAELRQAVARRQFIVYYQPQVTYE-GDVVGAEALVRWQHPIRGIVSPTTF 573 A A Q+ Q +LR A+ R QF + YQP+ + G V+GAEAL+RW+HP+ G+V+P F Sbjct: 431 NANAQQQLQWLQDLRVALERGQFRLVYQPKFSLPAGPVLGAEALLRWEHPVHGLVAPDQF 490 Query: 574 ISLAEDMGLMDEIGRQVLHRICSDLENWQREGLARDLVVAINVSAQEFKVDNFVYRFREI 633 I LAE GL+ IG VL C + W G D +++N+SA +F + + R Sbjct: 491 IGLAERSGLILPIGAWVLDEACRQMREWFALGYV-DWTMSVNLSALQFADPHLLEVVRTT 549 Query: 634 MREHHVPSHSVKVELTESMMVDHIEDVITKMQALRSNEVSISLDDFGTGYSSLSYLGRFP 693 + H +P + +E+TES + E + ++ + V IS+DDFGTGYSSL YL R P Sbjct: 550 LERHQLPPPCLTLEVTESTAMHDTEASLQTLRQIADLGVEISIDDFGTGYSSLLYLKRLP 609 Query: 694 IDQIKIDQTFVREMQNDAAMASIIRSIIMLGQSLNLSIIAEGVETEQQRDFLHAQGCLLY 753 +++KID+ FVRE++ D A+I+ +I+ LG++LNL I+AEGVET +Q+DFL A GC Sbjct: 610 ANELKIDRGFVRELRQDGEDAAIVSAIVALGRTLNLRIVAEGVETVEQQDFLTAAGCDAL 669 Query: 754 QGYWYSKPIPGEQF 767 QGY +P+ E F Sbjct: 670 QGYLMGRPMTPELF 683 >gi|300312026|ref|YP_003776118.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1106 Score = 273 bits (697), Expect = 5e-74, Method: Compositional matrix adjust. Identities = 154/439 (35%), Positives = 256/439 (58%), Gaps = 11/439 (2%) Query: 336 AENRIDQLAFYDPVTQLPNKKLFMDRLRRGLDRRRTSGHAGAVFVMEIHRADLSYGSVKV 395 A+ RI LA YD +T LPN+ L + + AV +M++ R + Sbjct: 662 ADERIRHLAHYDELTGLPNRFLCTQHVSNAITHAEHRNGMVAVILMDLDRFKNINDTFGH 721 Query: 396 DEMEQLLIECARRFSSCITAEQTVARMEGEKFALGTEIRSNELAECIRYCEALAQRILDA 455 + E +L E A+RF + + +AR+ G++F + ++ + ++ E +AQ++L Sbjct: 722 ETGEAVLQEIAQRFRNSLRELDILARVGGDEFI----VLVDDFDDPVQLGE-IAQKLLTE 776 Query: 456 FTDPQSLSAARFPCSVNLGINIVRDEQYGASELVMQAELAMVQSRAAGRNVFRFFDPAMQ 515 P + SV++GI + A L+ A++AM +++ G++ +RFF + Sbjct: 777 ARKPFIIDGQEAMLSVSIGIATYPGDGDNAQTLIKNADIAMYRAKHHGKDDYRFFSDEIN 836 Query: 516 ALANQRSQLEAELRQAVARRQFIVYYQPQVTY-EGDVVGAEALVRWQHPIRGIVSPTTFI 574 +R LEAELR+A+ R +F+V+YQP++ EG +VGAEALVRWQHP+RG++ P FI Sbjct: 837 TNTVERIALEAELRRAIERAEFVVHYQPKIELAEGRIVGAEALVRWQHPLRGLLPPGEFI 896 Query: 575 SLAEDMGLMDEIGRQVLHRICSD--LENWQREGLARDLVVAINVSAQEFKVDNFVYRFRE 632 +LAE+ L+D+IG VL C D L Q+ R ++IN++ +F + + +E Sbjct: 897 TLAEETRLIDQIGLLVLDVACRDIGLMRQQQMTCGR---ISINLTGSQFGDERLLDDIKE 953 Query: 633 IMREHHVPSHSVKVELTESMMVDHIEDVITKMQALRSNEVSISLDDFGTGYSSLSYLGRF 692 ++ H P ++ E+TESM++ + E I M +R+ +IS+DDFGTGYSSL+YL RF Sbjct: 954 VVDRHGTPPSCLEFEITESMVMHNRERAIAIMDGIRAEGFTISIDDFGTGYSSLAYLKRF 1013 Query: 693 PIDQIKIDQTFVREMQNDAAMASIIRSIIMLGQSLNLSIIAEGVETEQQRDFLHAQGCLL 752 P++ +KID++F+ ++ ++ ++I+++II + +LNL ++ EGVETE+Q L G Sbjct: 1014 PVNTLKIDKSFINDIPDNPNSSAIVQAIIAMSHALNLKVVTEGVETEKQLQALREFGSDE 1073 Query: 753 YQGYWYSKPIPGEQFADLL 771 YQGY++SKP+P QF LL Sbjct: 1074 YQGYYFSKPVPYAQFVQLL 1092 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.135 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,999,007 Number of extensions: 74520 Number of successful extensions: 367 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 325 Number of HSP's successfully gapped: 19 Length of query: 778 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 685 Effective length of database: 1,179,515 Effective search space: 807967775 Effective search space used: 807967775 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)