BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0334.p (316 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313598|ref|YP_003777690.1| D-3-phosphoglycerate dehydrogen... 635 0.0 gi|300310832|ref|YP_003774924.1| D-isomer specific 2-hydroxyacid... 130 2e-31 gi|300310960|ref|YP_003775052.1| dehydrogenase oxidoreductase [H... 124 8e-30 gi|300313735|ref|YP_003777827.1| D-3-phosphoglycerate dehydrogen... 123 2e-29 gi|300311826|ref|YP_003775918.1| D-lactate dehydrogenase [Herbas... 108 4e-25 >gi|300313598|ref|YP_003777690.1| D-3-phosphoglycerate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 328 Score = 635 bits (1639), Expect = 0.0, Method: Compositional matrix adjust. Identities = 311/316 (98%), Positives = 314/316 (99%) Query: 1 VRHLDCFRLLDGHDVKVFNNTIRGVGQLAARLSPFDAVVLIRERTALTRPLLEKLPLLKL 60 VRHLDCFRLLDGHDVKVFNNTIRGVGQLAARLSPFDAVVLIRERTALTRPLLEKLPLLKL Sbjct: 13 VRHLDCFRLLDGHDVKVFNNTIRGVGQLAARLSPFDAVVLIRERTALTRPLLEKLPLLKL 72 Query: 61 VSQTGKVSGHVDVAACTERGIAIVEGVGDPTAPAELTWALIMAAMRRVPQYCSALRAGAW 120 VSQTGKVSGHVDVAACTERGIAIVEGVGDPTAPAELTWALIMAAMRRVPQYCSALRAGAW Sbjct: 73 VSQTGKVSGHVDVAACTERGIAIVEGVGDPTAPAELTWALIMAAMRRVPQYCSALRAGAW 132 Query: 121 QQVSPTPHHNLIGTALKGRTLGIWGYGKIGRLVAGYGRAFGMQVLVWGRDASREQAIKDG 180 QQVSPTPHHNLIGTALKGRTLGIWGYGKIGRLVAGYGRAFGMQVLVWGRD+SREQAIKDG Sbjct: 133 QQVSPTPHHNLIGTALKGRTLGIWGYGKIGRLVAGYGRAFGMQVLVWGRDSSREQAIKDG 192 Query: 181 YQAAASKEDFFAQADVLSLHLRLNDATRGIVTASDLARMKATACFVNTSRAELVEPDALE 240 YQAAASKE+FFAQADVLSLHLRLNDATRGIV ASDLARMKATACFVNTSRAELVEPDALE Sbjct: 193 YQAAASKEEFFAQADVLSLHLRLNDATRGIVKASDLARMKATACFVNTSRAELVEPDALE 252 Query: 241 AALTAGRPGLAALDVYEREPLPVESALLKMDNVVAAPHLGYVEKDGYELYFRAAFQNIVA 300 AAL AGRPGLAALDVYEREPLPVESALL+MDNVVAAPHLGYVEKDGYELYFRAAFQNIVA Sbjct: 253 AALIAGRPGLAALDVYEREPLPVESALLQMDNVVAAPHLGYVEKDGYELYFRAAFQNIVA 312 Query: 301 YAAGAPTHVLNPEALG 316 YAAGAPTHVLNPEALG Sbjct: 313 YAAGAPTHVLNPEALG 328 >gi|300310832|ref|YP_003774924.1| D-isomer specific 2-hydroxyacid dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 319 Score = 130 bits (327), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 95/265 (35%), Positives = 135/265 (50%), Gaps = 10/265 (3%) Query: 47 LTRPLLEKLPLLKLVSQTGKVSGHVDVAACTERGIAIVEGVGDPT-APAELTWALIMAAM 105 +T +LE P LK+VS + D+ +RG+ + G T A A+ +ALI+A+ Sbjct: 56 ITNAMLEAAPQLKIVSTISVGVDNFDLDYFRQRGLMLAHTPGVLTEATADTIFALILASA 115 Query: 106 RRVPQYCSALRAGAWQQVSPTPHHNLIGTALKGRTLGIWGYGKIGRLVAGYGR-AFGMQV 164 RRV + ++AG W+ + G + G+TLG+ G G+IG VA FGM + Sbjct: 116 RRVVELAEYVKAGRWKG---SIGEAQFGVNVHGKTLGLIGMGRIGSAVARRAHHGFGMPI 172 Query: 165 LVWGR--DASREQAIKDGYQAAASKEDFFAQADVLSLHLRLNDATRGIVTASDLARMKAT 222 L R D E+ + Y SKED AQAD + + L L T + A + A MK + Sbjct: 173 LYHNRRPDPEAERELGARY---VSKEDLLAQADFVCVMLPLTPQTERMFGAPEFALMKRS 229 Query: 223 ACFVNTSRAELVEPDALEAALTAGRPGLAALDVYEREPLPVESALLKMDNVVAAPHLGYV 282 A F+N SR +V+ AL AAL A LDV+E EPLP++S LLK+ NVVA PH+G Sbjct: 230 AIFINASRGRIVDEAALIAALQDKTIHGAGLDVFEVEPLPLQSPLLKLPNVVALPHIGSA 289 Query: 283 EKDGYELYFRAAFQNIVAYAAGAPT 307 + A N++A G P Sbjct: 290 THETRLAMAELAVTNLIAGLRGEPV 314 >gi|300310960|ref|YP_003775052.1| dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 326 Score = 124 bits (312), Expect = 8e-30, Method: Compositional matrix adjust. Identities = 93/273 (34%), Positives = 136/273 (49%), Gaps = 9/273 (3%) Query: 47 LTRPLLEKLPLLKLVSQTGKVSGHVDVAACTERGIAIVE--GVGDPTAPAELTWALIMAA 104 +T L P LK V ++DVAA T+ G+ V + T A+ WAL+MAA Sbjct: 57 VTAALFAANPQLKAVCNMAVGYNNIDVAAATQAGVMATNTPDVLNETT-ADFGWALMMAA 115 Query: 105 MRRVPQYCSALRAGAWQQVSPTPHHNLIGTALKGRTLGIWGYGKIGRLVAGYGRAFGMQV 164 RR+ + LRAG W++ S + + +G + G TLGI G G+IG+ +A F MQV Sbjct: 116 ARRITESEHFLRAGKWKKWS---YDSFVGPDIHGATLGIIGMGRIGQAIARRSLGFDMQV 172 Query: 165 LVWGRD-ASREQAIKDGYQAAASKEDFFAQADVLSLHLRLNDATRGIVTASDLARMKATA 223 L R + E + + SKE+ QAD + L L + T + A++LA MK TA Sbjct: 173 LYHNRSRLAPELEARANHARYVSKEELLRQADHVILVLPYSKDTHHTIAAAELALMKPTA 232 Query: 224 CFVNTSRAELVEPDALEAALTAGRPGLAALDVYEREPLPVESALLKMDNVVAAPHLGYVE 283 N +R +V+ AL AAL GR A +DV+E EP + L + NVV PH+ Sbjct: 233 TLTNIARGGIVDDAALIAALREGRIAAAGVDVFENEP-AFKPEFLDLSNVVLTPHIASAS 291 Query: 284 KDGYELYFRAAFQNIVAYAAGA-PTHVLNPEAL 315 A N++A +G P ++LNP+ + Sbjct: 292 TPTRLAMANCAADNLIAALSGQRPPNLLNPDVV 324 >gi|300313735|ref|YP_003777827.1| D-3-phosphoglycerate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 308 Score = 123 bits (308), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 92/267 (34%), Positives = 137/267 (51%), Gaps = 17/267 (6%) Query: 38 VVLIRERTALTRPLLEKLPLLKLVSQTGKVSGHVDVAACTERGIAIVEGVGDPTAPA-EL 96 V +I ++ +++ P+L+++S+ G +D A +RGIA+ G E Sbjct: 51 VAIIVRYGGVSARVMDAAPILRVISKHGTGIDSIDSQAAQQRGIAVKAAAGANAPAVAEH 110 Query: 97 TWALIMAAMRRVPQYCSALRAGAWQQVSPTPHHNLIGTALKGRTLGIWGYGKIGRLVAGY 156 TWALIMA + V +R G W + + H +L L+GRTLG+ G G IGR VA Sbjct: 111 TWALIMACAKNVVGLDQRMREGHWDK---STHKSL---ELQGRTLGLVGLGAIGRRVAAV 164 Query: 157 GRAFGMQVLV---WGRDASREQAIKDGYQAAASKEDFFAQADVLSLHLRLNDATRGIVTA 213 A GM VL + ++A + + D + FAQ+DV+SLH L + ++ A Sbjct: 165 AAALGMPVLAHDPYAKEAPQGVQLVD-------LDTLFAQSDVVSLHCPLTAENKHMINA 217 Query: 214 SDLARMKATACFVNTSRAELVEPDALEAALTAGRPGLAALDVYEREPLPVESALLKMDNV 273 LARMK A VNT+R L++ AL AAL +G+ A LD +E+EP L ++ N Sbjct: 218 QSLARMKDGAILVNTARGGLIDEQALIAALDSGKLRAAGLDSFEKEPFTAPHPLQRVGNA 277 Query: 274 VAAPHLGYVEKDGYELYFRAAFQNIVA 300 V +PH+G V D Y A N++A Sbjct: 278 VLSPHIGGVTSDAYIAMGTGAASNVLA 304 >gi|300311826|ref|YP_003775918.1| D-lactate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 330 Score = 108 bits (271), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 98/311 (31%), Positives = 141/311 (45%), Gaps = 31/311 (9%) Query: 12 GHDVKVFNNTIR-GVGQLAARLSPFDAVVLIRERTALTRPLLEKLPLLKLVSQTGKVSG- 69 GH++ + + G QLAA A V L +LEKL L + T + +G Sbjct: 24 GHELVFLQDRLEAGTAQLAAGCQAVAAFV----NDDLGGVVLEKLAELGVRFVTTRSTGF 79 Query: 70 -HVDVAACTERGIAIVEGVGD--PTAPAELTWALIMAAMRRVPQYCSALRAGAWQQVSPT 126 H+DVAA G+ + V D P + AE L+ A R++ + R G ++ Sbjct: 80 NHIDVAAARALGLTVAR-VADYSPYSVAEFAVGLLQAVNRKIARASMRTREGNFEL---- 134 Query: 127 PHHNLIGTALKGRTLGIWGYGKIGRLVAGYGRAFGMQVLVWGRDASREQAIKDGYQAAAS 186 L+G L G+T+G+ G GKIG L A FG Q+L G D R Sbjct: 135 --DGLMGVDLHGKTVGVIGTGKIGVLFARIMAGFGCQIL--GSDVHRNPDFDALGGRYVE 190 Query: 187 KEDFFAQADVLSLHLRLNDATRGIVTASDLARMKATACFVNTSRAELVEPDALEAALTAG 246 + + FA +DV+SLH L +ATR +V A LA K VNTSR LV+ +A AAL +G Sbjct: 191 RTELFAASDVISLHCPLTEATRYLVNAQSLALAKPGCILVNTSRGGLVDTEAAIAALKSG 250 Query: 247 RPGLAALDVYEREPL-------------PVESALLKMDNVVAAPHLGYVEKDGYELYFRA 293 R A+DVYE+E V L+ NV+ H + ++ + Sbjct: 251 RLRGLAIDVYEQEASLFFQDLSSTIITDDVIQRLVSFPNVIVTGHQAFFTEEAIGQIMQT 310 Query: 294 AFQNIVAYAAG 304 +N+ A+AAG Sbjct: 311 TIENLSAFAAG 321 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 992,854 Number of extensions: 40873 Number of successful extensions: 179 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 158 Number of HSP's successfully gapped: 11 Length of query: 316 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 231 Effective length of database: 1,217,395 Effective search space: 281218245 Effective search space used: 281218245 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)