BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0315.p (387 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313580|ref|YP_003777672.1| outer membrane porin [Herbaspir... 697 0.0 gi|300309527|ref|YP_003773619.1| outer membrane protein (porin) ... 500 e-142 gi|300310328|ref|YP_003774420.1| porin signal peptide protein [H... 299 3e-82 gi|300312191|ref|YP_003776283.1| outer membrane porin [Herbaspir... 251 6e-68 gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) ... 173 2e-44 >gi|300313580|ref|YP_003777672.1| outer membrane porin [Herbaspirillum seropedicae SmR1] Length = 387 Score = 697 bits (1799), Expect = 0.0, Method: Compositional matrix adjust. Identities = 379/387 (97%), Positives = 382/387 (98%) Query: 1 MKKSLLALAVLGAFAGAAQAQSSVTIYGIVDTGIAYSSKVGYTDANGNAATGSKFGINSG 60 MKKSLLALAVLGAFAGAAQAQSSVTIYGIVDTGIAYSSKVGYTDANGNAATGSKFGINSG Sbjct: 1 MKKSLLALAVLGAFAGAAQAQSSVTIYGIVDTGIAYSSKVGYTDANGNAATGSKFGINSG 60 Query: 61 VIQGSRIGFKGVEDLGGGLSAVFNLETGFTSDDGGLQGSDAVTSSNLFRRKSVVGLSGGF 120 VIQGSRIGFKGVEDLGGGLSAVFNLETGFTSDDGGLQGSDAVTSSNLFRRKSVVGLSGGF Sbjct: 61 VIQGSRIGFKGVEDLGGGLSAVFNLETGFTSDDGGLQGSDAVTSSNLFRRKSVVGLSGGF 120 Query: 121 GTVLLGRQTDFADTISAYTAVADFGGVVANSGSSLNRLQGTRTNNSVSYTTANLSGFTGN 180 GTVLLGRQTDFADTISAYTAVADFGGVVANSGSSLNRLQGTRTNNSVSYTTANLSGFTGN Sbjct: 121 GTVLLGRQTDFADTISAYTAVADFGGVVANSGSSLNRLQGTRTNNSVSYTTANLSGFTGN 180 Query: 181 LIYGFGEQAGKTSAGQAYGIGGKYDNGPLGLGINYYQSKAGSTPSDTSLIPAAGATNANT 240 LIYGFGEQAGKTSAGQAYGIGGKYDNGPLGLGINYYQSKAGSTPSDTSLIPAAGATNANT Sbjct: 181 LIYGFGEQAGKTSAGQAYGIGGKYDNGPLGLGINYYQSKAGSTPSDTSLIPAAGATNANT 240 Query: 241 ALGVAGNTALKSLNVVASYQFGPARVYANYSRVKQDLKGTPTVATATLGNAQKVDTYEIG 300 ALGVAGNTALKSLNVVASYQFGPARVYANYSRVKQDLKGTPTVATATL NAQK DTYEIG Sbjct: 241 ALGVAGNTALKSLNVVASYQFGPARVYANYSRVKQDLKGTPTVATATLANAQKADTYEIG 300 Query: 301 TAYSLSPSLKLLAAVDHSRATFTGVDGKGKLTQISLGADYSLSKRTDLYAFVSNMRATDI 360 TAYSLSPSLKLLAAVDHSRATF GV+ KGKLTQISLGADY LSKRTDLYAFVSNMRA+DI Sbjct: 301 TAYSLSPSLKLLAAVDHSRATFNGVNTKGKLTQISLGADYFLSKRTDLYAFVSNMRASDI 360 Query: 361 QNPGVTGGATGSDASQTAVITGIRHKF 387 QNPGVTGGATGSDASQTA+ITGIRHKF Sbjct: 361 QNPGVTGGATGSDASQTAIITGIRHKF 387 >gi|300309527|ref|YP_003773619.1| outer membrane protein (porin) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 390 Score = 500 bits (1287), Expect = e-142, Method: Compositional matrix adjust. Identities = 279/398 (70%), Positives = 316/398 (79%), Gaps = 19/398 (4%) Query: 1 MKKSLLALAVLGAFAGAAQAQSSVTIYGIVDTGIAYSSKVGYTDANGNAATGSKFGINSG 60 MKKSLLALAVLGAFAGAAQAQSSVTIYGIVDTG+ Y SKV A TGSKF +NSG Sbjct: 1 MKKSLLALAVLGAFAGAAQAQSSVTIYGIVDTGVVYQSKV----ATPTGGTGSKFSLNSG 56 Query: 61 VIQGSRIGFKGVEDLGGGLSAVFNLETGFTSDDGGLQGSDAVTSSNLFRRKSVVGLSGGF 120 VIQGSRIGFKG EDLGGGLSAVFNLE GF++D G L D+ TS+ LFRRKSVVGL+G F Sbjct: 57 VIQGSRIGFKGTEDLGGGLSAVFNLEAGFSNDTGALD--DSKTSNTLFRRKSVVGLAGNF 114 Query: 121 GTVLLGRQTDFADTISAYTAVADFGGVVANSGSSLNRLQGTRTNNSVSYTTANLSGFTGN 180 G+VLLGRQTDFADTIS+YT+V DFGG +G +L+RL+GTRTNNS+SYTTANL+GFTGN Sbjct: 115 GSVLLGRQTDFADTISSYTSVQDFGGWTGAAGHNLDRLEGTRTNNSISYTTANLNGFTGN 174 Query: 181 LIYGFGEQAGKTSAGQAYGIGGKYDNGPLGLGINYYQSKAGSTPSDTSLIPAAGATNANT 240 L+YGFGE AG+TSAGQA+G+GGKYDNGPLGLGINYYQSKAGSTP+D SL+ A G TN T Sbjct: 175 LMYGFGETAGQTSAGQAFGVGGKYDNGPLGLGINYYQSKAGSTPADASLLAATG-TNQFT 233 Query: 241 ALGVAGNTALKSLNVVASYQFGPARVYANYSRVKQDLK-----------GTPTVATATLG 289 G+TA K LNV ASYQFGPARVY N+SRVKQDL T++ TL Sbjct: 234 NGTNNGSTAQKVLNVAASYQFGPARVYGNWSRVKQDLNTQANLTAAGVGSLATISNRTLA 293 Query: 290 NAQKVDTYEIGTAYSLSPSLKLLAAVDHSRATFTGVDGKGKLTQISLGADYSLSKRTDLY 349 + K D YEIGTAYSLSPSLKLLAAVDH+RA F GV GK KLTQ+SLG DY LSKRTDLY Sbjct: 294 ASNKADIYEIGTAYSLSPSLKLLAAVDHTRAQFDGVSGKAKLTQVSLGTDYWLSKRTDLY 353 Query: 350 AFVSNMRATDIQNPGVTGGATGSDASQTAVITGIRHKF 387 AF++ +ATD+ NPGV ++G+D +Q AV GIRHKF Sbjct: 354 AFLAYSKATDLNNPGV-ADSSGTDGNQGAVAVGIRHKF 390 >gi|300310328|ref|YP_003774420.1| porin signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 405 Score = 299 bits (765), Expect = 3e-82, Method: Compositional matrix adjust. Identities = 187/417 (44%), Positives = 250/417 (59%), Gaps = 42/417 (10%) Query: 1 MKKSLLALAVLGAFAGAAQAQSS-VTIYGIVDTGIAYSSKVGYTDANGNAATGSKFGINS 59 MKKSLLALAVLGAFAGAA AQSS +TIYG +D + Y++K+ N TGS + S Sbjct: 1 MKKSLLALAVLGAFAGAAHAQSSSITIYGAIDASLTYTNKIATDGKN----TGSLTAVES 56 Query: 60 GVIQGSRIGFKGVEDLGGGLSAVFNLETGFTSDDGGLQGSDAVTSSNLFRRKSVVGLSGG 119 G+++GSR+G KG EDLGGGL AVF LE GF D G +A ++ R++ VGLSG Sbjct: 57 GLVKGSRLGIKGSEDLGGGLKAVFTLENGFNVDTG-----EAGQGKLMWGRQTTVGLSGN 111 Query: 120 FGTVLLGRQTDFADTISAYTAVADFG--GVVANSGSSLNRLQGTRTNNSVSYTTANLSGF 177 FGTVL+GRQ D + I + FG G V + G L+R G R NNS+ Y + N +G Sbjct: 112 FGTVLVGRQNDPLNDIGGIASAGGFGVPGTVHSKG--LDRTDGQRLNNSIRYNSPNFNGL 169 Query: 178 TGNLIYGFGEQAGKTSAGQAYGIGGKYDNGPLGLGINYYQSKAGSTPSDTSLIPAAGATN 237 + + IYGFGEQ G+ G++ G+G +Y NGPL LG+ Y+QSK G +S + A A N Sbjct: 170 SFSGIYGFGEQVGRAVGGRSIGLGSQYVNGPLSLGLGYFQSKMGGGSDGSSDVNAKAACN 229 Query: 238 ANTALGVAGNTALKSLNVVASYQFGPARVYANYSRVKQDLK------------------- 278 AG+T LK+ + A+YQFGPA+VY +S V+ L Sbjct: 230 GAGEKDHAGDTCLKTWTLAAAYQFGPAKVYGAFSHVRLPLAKLHTKVRKDNNFGAMYDAK 289 Query: 279 -GT---PTVATA-TLG--NAQKVDTYEIGTAYSLSPSLKLLAAVDHSRATFTGVDGKGKL 331 GT P+ ATA ++G N + T++IG +Y L PSL L++AV H+RA F G G+L Sbjct: 290 TGTGYNPSDATAFSIGGANHKSSQTFDIGMSYQLMPSLTLMSAVQHTRAKFVGA-ASGRL 348 Query: 332 TQISLGADYSLSKRTDLYAFVSNMRATDIQNPGVTGG-ATGSDASQTAVITGIRHKF 387 Q++LGA Y LSKRT YA V N+R++D+ N G++ G D SQTA+ TG+ H F Sbjct: 349 LQLNLGAKYDLSKRTSAYALVRNLRSSDMYNVGLSKDQEPGIDRSQTAISTGLIHTF 405 >gi|300312191|ref|YP_003776283.1| outer membrane porin [Herbaspirillum seropedicae SmR1] Length = 372 Score = 251 bits (642), Expect = 6e-68, Method: Compositional matrix adjust. Identities = 156/378 (41%), Positives = 221/378 (58%), Gaps = 45/378 (11%) Query: 24 VTIYGIVDTGIAYSSKVGYTDANGNAATGSKFGINSGVIQGSRIGFKGVEDLGGGLSAVF 83 VT+YG++D + Y+SKVG + GS+ ++SG SRIG +G E+LG GL+A+F Sbjct: 26 VTVYGVIDMSLVYTSKVGPDN-------GSRASLDSGDAMASRIGLRGREELGDGLAAIF 78 Query: 84 NLETGFTSDDGGLQGSDAVTSSNLFRRKSVVGLSGGFGTVLLGRQTDFADTI-SAYTAVA 142 LETGF DDG + T LF RKSVVGL+G FGT+ +GRQTD+ + I YT+ Sbjct: 79 QLETGFAGDDGAM-----ATPGTLFDRKSVVGLAGRFGTLTVGRQTDYLEEIGQQYTSFQ 133 Query: 143 DFGG--VVANSGSSLNRLQGTRTNNSVSYTTANLSGFTGNLIYGFGEQAGKTSAGQAYGI 200 FGG V A +SL+R+ G R+NNS+ + TA G G+ YG GE AG T +GQ+ G+ Sbjct: 134 IFGGGGVRAAHFNSLDRITGARSNNSLRFDTAEYGGLRGSFYYGAGEVAGSTRSGQSLGV 193 Query: 201 GGKYDNGPLGLGINYYQSKAGSTPSDTSLIPAAGATNANTALGVAGNTALKSLNVVASYQ 260 GG + G +G+G Y+QSK S +PA AG+T LK+ + ASYQ Sbjct: 194 GGNFRRGNVGVGAAYFQSKLASDA-----LPAR-----------AGDTELKTFTLGASYQ 237 Query: 261 FGPARVYANYSRVK----QDLKGTPTVATATLGNAQKVDTYEIGTAYSLSPSLKLLAAVD 316 GPA+++A +S+++ Q L + VA + +A + + ++G Y+L L LLA+V Sbjct: 238 AGPAKLFAAWSQIRRPTQQPLASSGLVA---ITSATRANVLDVGVDYALDSKLHLLASVI 294 Query: 317 HSRATFTGVDG---KGKLTQISLGADYSLSKRTDLYAFVSNMRATDIQNPGVTGGATGS- 372 H RA + G +G TQ++LG D LSKRTD+YA S +A D+ NPG+ A S Sbjct: 295 HDRADLSRAAGGATRGSTTQVNLGVDLLLSKRTDVYAMASRQQARDVLNPGIISAAYSSA 354 Query: 373 ---DASQTAVITGIRHKF 387 D+SQ + G+RHKF Sbjct: 355 PADDSSQNVLRVGLRHKF 372 >gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 352 Score = 173 bits (438), Expect = 2e-44, Method: Compositional matrix adjust. Identities = 150/402 (37%), Positives = 201/402 (50%), Gaps = 65/402 (16%) Query: 1 MKKSLLALAVLGAFAGAAQAQSSVTIYGIVDTGIAYSSKVGYTDANGNAATGSKF-GINS 59 MKK+LL A+L A A AQSSV+IYG++D G+ Y Y+ A + G++ ++S Sbjct: 1 MKKTLLVAAMLAGGATTAHAQSSVSIYGLIDLGLTY-----YSSAPKSGGGGARLLRMDS 55 Query: 60 GVIQGSRIGFKGVEDLGGGLSAVFNLETGFTSDDGGL-QGSDAVTSSNLFRRKSVVGL-S 117 G+ QGSRIGFKG EDLGGG++A F LETGF +DDG L QG LF R+S VGL S Sbjct: 56 GIAQGSRIGFKGTEDLGGGMNAFFTLETGFGADDGSLGQGGL------LFGRQSFVGLGS 109 Query: 118 GGFGTVLLGRQTDFADTI------SAYTAVADFG-GVVANS--GSSLNR--LQGTRTNNS 166 +G++ +GRQ DF I A + FG G+ A++ G++LN G RTNNS Sbjct: 110 TQWGSLSVGRQYDFMANIGGQYAMGALSPAGSFGWGLHADAAHGTALNNHIYAGDRTNNS 169 Query: 167 VSYTTANLSGFTGNLIYGFGEQAGKTSAGQAYGIGGKYDNGPLGLGINYYQSKAGSTPSD 226 + Y +A GF+ + G GE AG +AG+ G+YDNGP + Y K ST S Sbjct: 170 IKYNSAKFGGFSFGAMVGLGETAGNNNAGRTVSATGQYDNGPFSGALAYTDIKD-STGSF 228 Query: 227 TSLIPAAGATNANTALGVAGNTALKSLNVVASYQFGPARVYANYSRVKQDLKGTPTVATA 286 ++ I G A YQ G + ++VK T T A A Sbjct: 229 STRIYGGG----------------------ARYQIGAFTPFGVVTQVKN----TNTSAKA 262 Query: 287 TLGNAQKVDTYEIGTAYSLSPSLKLLAAVDHSRATFTGVDGKGKLTQISLGADYSLSKRT 346 T TYE+GT Y+L+P L AA + G G + DY +SKRT Sbjct: 263 T--------TYELGTVYALTPLWDLAAAYQFQKRN----QGVGNAQALVATVDYKISKRT 310 Query: 347 DLYAFVSNMRATDIQ-NPGVTGGATGSDASQTAVITGIRHKF 387 DLY R P GG + +QTA+ G+RH+F Sbjct: 311 DLYVGAVYDRDKGYNAYPVFGGGIQSATGNQTALRLGMRHRF 352 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.312 0.130 0.359 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,115,522 Number of extensions: 48690 Number of successful extensions: 220 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 162 Number of HSP's successfully gapped: 14 Length of query: 387 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 300 Effective length of database: 1,207,925 Effective search space: 362377500 Effective search space used: 362377500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 131 (55.1 bits)