BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0282.p (506 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313549|ref|YP_003777641.1| ABC transporter ATPase [Herbasp... 1020 0.0 gi|300310063|ref|YP_003774155.1| sugar ABC transporter ATPase [H... 273 2e-74 gi|300310382|ref|YP_003774474.1| sugar ABC transporter ATPase [H... 272 5e-74 gi|300310741|ref|YP_003774833.1| ABC transporter ATPase [Herbasp... 263 3e-71 gi|300310371|ref|YP_003774463.1| sugar ABC transporter ATPase [H... 248 7e-67 >gi|300313549|ref|YP_003777641.1| ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 518 Score = 1020 bits (2638), Expect = 0.0, Method: Compositional matrix adjust. Identities = 503/506 (99%), Positives = 503/506 (99%) Query: 1 VRKAYPGVLANDDVSLAVAPGEIHAVLGENGAGKSTLMKIIFGAVKPDAGEIRWNGEAVQ 60 VRKAYPGVLANDDVSLAVAPGEIHAVLGENGAGKSTLMKIIFGAVKPDAGEIRWNGEAVQ Sbjct: 13 VRKAYPGVLANDDVSLAVAPGEIHAVLGENGAGKSTLMKIIFGAVKPDAGEIRWNGEAVQ 72 Query: 61 IANPQVARKLGIAMVFQHFSLFDTLTVTENIALGLDAKTDLKELAERITRTGEKYGLELE 120 IANPQVARKLGIAMVFQHFSLFDTLTVTENIALGLDAKTDLKELAERITRTGEKYGLELE Sbjct: 73 IANPQVARKLGIAMVFQHFSLFDTLTVTENIALGLDAKTDLKELAERITRTGEKYGLELE 132 Query: 121 PQRHVHTLSVGERQRVEIVRALLTEPQLLILDEPTSVLTPQAVEKLFVTLRRLADEGCSI 180 PQRHVHTLSVGERQRVEIVRALLTEPQLLILDEPTSVLTPQAVEKLFVTLRRLADEGCSI Sbjct: 133 PQRHVHTLSVGERQRVEIVRALLTEPQLLILDEPTSVLTPQAVEKLFVTLRRLADEGCSI 192 Query: 181 LYISHKLDEIRALCHSATVMRGGRVTGNCDPRNESSASLSRMMIGDELVRLQRERNPESM 240 LYISHKLDEIRALCHSATVMRGGRVTGNCDPRNESSASLSRMMIGDELVRLQRERNPESM Sbjct: 193 LYISHKLDEIRALCHSATVMRGGRVTGNCDPRNESSASLSRMMIGDELVRLQRERNPESM 252 Query: 241 RNERKLHVNRLSLPKAHLFATELKDIECEVRAGEIVGIAGVSGNGQQELLAALSGEDQRA 300 RNERKLHVNRLSLPKAHLFATELKDIECEVRAGEIVGIAGVSGNGQQELLAALSGEDQRA Sbjct: 253 RNERKLHVNRLSLPKAHLFATELKDIECEVRAGEIVGIAGVSGNGQQELLAALSGEDQRA 312 Query: 301 APHMIMLGGKAVGDLSPNPRRAAGLGLVPEERLGRGAVPTLSLTANMLLSHQKAPYVKRG 360 APHMIMLG KAVGDLSPNPRRAAGLGLVPEERLGRGAVPTLSLTANMLLSHQKAPYVKRG Sbjct: 313 APHMIMLGSKAVGDLSPNPRRAAGLGLVPEERLGRGAVPTLSLTANMLLSHQKAPYVKRG 372 Query: 361 MIDFGFTRRAAASIIERFKVKAAGPDAPAKSLSGGNLQKFIVGREMERKPAVFVVAQPTW 420 MIDFGFTRRAAASIIERFKVKAAGPDAPAKSLSGGNLQKFIVGREMERKPAVFVVAQPTW Sbjct: 373 MIDFGFTRRAAASIIERFKVKAAGPDAPAKSLSGGNLQKFIVGREMERKPAVFVVAQPTW 432 Query: 421 GVDVGAAAQIHGEILKLKQEGCAVLVISEELDELFALCDRLHVIAKGRLSPSTPIEQATR 480 GVDVGAAAQIHGEILKLKQEGCAVLVISEELDELFALCDRLHVIAKGRLSPS PIEQATR Sbjct: 433 GVDVGAAAQIHGEILKLKQEGCAVLVISEELDELFALCDRLHVIAKGRLSPSIPIEQATR 492 Query: 481 EQVGVWMSGLWDEPAALDKGAEVGNG 506 EQVGVWMSGLWDEPAALDKG EVGNG Sbjct: 493 EQVGVWMSGLWDEPAALDKGTEVGNG 518 >gi|300310063|ref|YP_003774155.1| sugar ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 521 Score = 273 bits (699), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 168/482 (34%), Positives = 267/482 (55%), Gaps = 25/482 (5%) Query: 1 VRKAYPGVLANDDVSLAVAPGEIHAVLGENGAGKSTLMKIIFGAVKPDAGEIRWNGEAVQ 60 +RK++ LA D+ L + PGEIHA++GENGAGKSTLMK++ G PD GEI +G V Sbjct: 12 IRKSFGATLALSDMHLTIRPGEIHALMGENGAGKSTLMKVLSGVHAPDQGEILLDGRPVA 71 Query: 61 IANPQVARKLGIAMVFQHFSLFDTLTVTENIALGLDAKT-----DLKELAERITRTGEKY 115 + +P +R GI +++Q ++ ++V N+ +G + +T D + R + Sbjct: 72 LRDPGASRAAGINLIYQELAVAPNISVAANVFMGSELRTRLGLIDHAAMRSRTDAVLRQL 131 Query: 116 GLELEPQRHVHTLSVGERQRVEIVRALLTEPQLLILDEPTSVLTPQAVEKLFVTLRRLAD 175 G LS+ E+Q+VEI RAL+ +++I+DEPT+ L+ + E+LF +RRL D Sbjct: 132 GAGFGASDLAGRLSIAEQQQVEIARALVHRSRIVIMDEPTAALSERETEQLFNVVRRLRD 191 Query: 176 EGCSILYISHKLDEIRALCHSATVMRGGRVTGNCDPRNESSASLSRMMIGDELVRLQRER 235 EG +I+YISH++ E+ AL TV+R G G S + +MM+G L Sbjct: 192 EGLAIIYISHRMAEVYALADRVTVLRDGSFVGELVRDEIDSERIVQMMVGRSL------- 244 Query: 236 NPESMRNERKLHVNRLSLPKAH----LFATELKDIECEVRAGEIVGIAGVSGNGQQELLA 291 E +++R + LP L +++ +VRAGE++G AG+ G G+ EL Sbjct: 245 -SEFYQHQRIAPADAAQLPTVMQVRALAGGKIRPASFDVRAGEVLGFAGLVGAGRTELAR 303 Query: 292 ALSGEDQRAAPHMIMLGGKAVGDLSPNPRRAAGLGLVPEERLGRGAVPTLSLTANMLLSH 351 L G D R+ I+L G+ V P AG+ VPE+R G+G +++ AN + + Sbjct: 304 LLFGADPRSGGD-ILLEGRPVHIDQPRAAMRAGIAYVPEDRKGQGLFLQMAVAANATM-N 361 Query: 352 QKAPYVKRGMI---DFGFTRRAAASIIERFKVKAAGPDAPAKSLSGGNLQKFIVGREMER 408 + + + G++ G RAA I+R VK A P+ P LSGGN QK ++ R +E Sbjct: 362 VASRHTRLGLVRSRSLGGVARAA---IQRLNVKVAHPETPVGKLSGGNQQKVLLARWLEI 418 Query: 409 KPAVFVVAQPTWGVDVGAAAQIHGEILKLKQEGCAVLVISEELDELFALCDRLHVIAKGR 468 P V ++ +PT GVD+ A ++I+ + +L +G AV+VIS EL E+ +CDR+ V+ +G Sbjct: 419 APKVLILDEPTRGVDIYAKSEIYQLVHRLASQGVAVVVISSELPEVIGICDRVLVMREGM 478 Query: 469 LS 470 ++ Sbjct: 479 IT 480 >gi|300310382|ref|YP_003774474.1| sugar ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 520 Score = 272 bits (695), Expect = 5e-74, Method: Compositional matrix adjust. Identities = 168/489 (34%), Positives = 275/489 (56%), Gaps = 15/489 (3%) Query: 1 VRKAYPGVLANDDVSLAVAPGEIHAVLGENGAGKSTLMKIIFGAVKPDAGEIRWNGEAVQ 60 V K +PGVLA D+ +A GE+HA++GENGAGKSTLMKI+ G + D+G+I +G+ V+ Sbjct: 28 VCKRFPGVLALDNCQFELAAGEVHALMGENGAGKSTLMKILSGVYQRDSGDILLDGKPVE 87 Query: 61 IANPQVARKLGIAMVFQHFSLFDTLTVTENIALGLDAK------TDLKELAERITRTGEK 114 I P+ A+ LGI ++ Q +L + L+ +NI +G + + D EL + + Sbjct: 88 ITEPRQAQALGIGIIHQELNLMNHLSAAQNIFIGREPRKAMGLFIDEDELNRQAAAIFAR 147 Query: 115 YGLELEPQRHVHTLSVGERQRVEIVRALLTEPQLLILDEPTSVLTPQAVEKLFVTLRRLA 174 L+++P V L+V +Q VEI +AL + ++LI+DEPT+ L + +LF +R L Sbjct: 148 MRLDMDPSTPVGELTVARQQMVEIAKALSFDSRVLIMDEPTAALNNAEIAELFRIIRDLQ 207 Query: 175 DEGCSILYISHKLDEIRALCHSATVMRGGRVTGNCDPRNESSASLSRMMIGDELVRLQRE 234 +G I+YISHK+DE+R + +VMR G+ + S ++ MM+G L QR Sbjct: 208 AQGVGIVYISHKMDELRQIADRVSVMRDGKYIATVPMQETSMDTIISMMVGRALDGEQR- 266 Query: 235 RNPESMRNERKLHVNRLSLPKAHLFATELKDIECEVRAGEIVGIAGVSGNGQQELLAALS 294 P++ RN+ L V L+ +A ++D+ +R GEI+G AG+ G G+ E+ A+ Sbjct: 267 IPPDTSRNDVVLEVRGLNRGRA------IRDVSFTLRKGEILGFAGLMGAGRTEVARAIF 320 Query: 295 GEDQRAAPHMIMLGGKAVGDLSPNPRRAAGLGLVPEERLGRGAVPTLSLTANMLLSHQKA 354 G D A +I+ GGKAV SP A G+G + E+R G + + AN+ LS Sbjct: 321 GADPLEAGEIIIHGGKAVIK-SPADAVAHGIGYLSEDRKHFGLAVGMDVQANIALSSM-G 378 Query: 355 PYVKRGMIDFGFTRRAAASIIERFKVKAAGPDAPAKSLSGGNLQKFIVGREMERKPAVFV 414 + + G +D R AA + + +K + A+ LSGGN QK ++ + + R + Sbjct: 379 RFTRVGFMDQRAIREAAQMYVRQLAIKTPSVEQQARLLSGGNQQKIVIAKWLLRDCDILF 438 Query: 415 VAQPTWGVDVGAAAQIHGEILKLKQEGCAVLVISEELDELFALCDRLHVIAKGRLSPSTP 474 +PT G+DVGA ++I+ + L ++G A+++IS EL E+ + R+ V+ +GR++ Sbjct: 439 FDEPTRGIDVGAKSEIYKLLDALAEQGKAIVMISSELPEVLRMSHRVLVMCEGRITGELA 498 Query: 475 IEQATREQV 483 AT+E++ Sbjct: 499 RADATQEKI 507 Score = 75.9 bits (185), Expect = 8e-15, Method: Compositional matrix adjust. Identities = 59/228 (25%), Positives = 110/228 (48%), Gaps = 8/228 (3%) Query: 263 LKDIECEVRAGEIVGIAGVSGNGQQELLAALSGEDQRAAPHMIMLGGKAVGDLSPNPRRA 322 L + + E+ AGE+ + G +G G+ L+ LSG QR + I+L GK V P +A Sbjct: 38 LDNCQFELAAGEVHALMGENGAGKSTLMKILSGVYQRDSGD-ILLDGKPVEITEPRQAQA 96 Query: 323 AGLGLVPEERLGRGAVPTLSLTANMLLSHQKAPYVKRGM-IDFGFTRRAAASIIERFKVK 381 G+G++ +E + LS N+ + + P G+ ID R AA+I R ++ Sbjct: 97 LGIGIIHQEL---NLMNHLSAAQNIFIGRE--PRKAMGLFIDEDELNRQAAAIFARMRLD 151 Query: 382 AAGPDAPAKSLSGGNLQKFIVGREMERKPAVFVVAQPTWGVDVGAAAQIHGEILKLKQEG 441 P P L+ Q + + + V ++ +PT ++ A++ I L+ +G Sbjct: 152 M-DPSTPVGELTVARQQMVEIAKALSFDSRVLIMDEPTAALNNAEIAELFRIIRDLQAQG 210 Query: 442 CAVLVISEELDELFALCDRLHVIAKGRLSPSTPIEQATREQVGVWMSG 489 ++ IS ++DEL + DR+ V+ G+ + P+++ + + + M G Sbjct: 211 VGIVYISHKMDELRQIADRVSVMRDGKYIATVPMQETSMDTIISMMVG 258 >gi|300310741|ref|YP_003774833.1| ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 522 Score = 263 bits (671), Expect = 3e-71, Method: Compositional matrix adjust. Identities = 186/508 (36%), Positives = 274/508 (53%), Gaps = 30/508 (5%) Query: 3 KAYPGVLANDDVSLAVAPGEIHAVLGENGAGKSTLMKIIFGAVKPDAGEIRWNGEAVQIA 62 K++ A D+VSL + G IHA+LGENGAGKSTL+K + G D G I + V I Sbjct: 13 KSFGSFRALDEVSLKIRAGTIHALLGENGAGKSTLVKGLVGYSPLDQGSILADRREVDIR 72 Query: 63 NPQVARKLGIAMVFQHFSLFDTLTVTENIALGLDAKTDLKELAERITRTGEKYGLE---- 118 + +V +LGI MV+QHF+L +LTV EN+ L A+ DL RI + E+ LE Sbjct: 73 SARVPNQLGIGMVYQHFTLAPSLTVAENLLL---ARGDLP---WRIRWSSERAVLEEFMA 126 Query: 119 -----LEPQRHVHTLSVGERQRVEIVRALLTEPQLLILDEPTSVLTPQAVEKLFVTLRRL 173 L P+R V +LS GE+Q++EI++ L + LILDEPTSVLTPQ +++ + L Sbjct: 127 KMPFKLSPERTVSSLSAGEKQKLEILKQLYLRRRFLILDEPTSVLTPQEADEVLGLMHAL 186 Query: 174 A-DEGCSILYISHKLDEIRALCHSATVMRGGRVTGNCDPRNESSASLSRMMIGDELV-RL 231 + ++L I+HK E+ A TV+R GR+ G+ S L+ M+G + Sbjct: 187 TRQQELTVLMITHKFHEVSAYADDVTVLRKGRLVGSARVAETSPDMLAHWMMGQARAEKA 246 Query: 232 QRERNPESMRNERKLHVNRLSLPKAHLFATELKDIECEVRAGEIVGIAGVSGNGQQELLA 291 Q R ++ L V L++ A ++ + +VR GEIVG+AG+SGNGQ+EL+ Sbjct: 247 QVARPAVPVQASVGLEVRDLTVNNDRGVAA-VRALSLQVRRGEIVGLAGISGNGQKELVE 305 Query: 292 ALSGEDQRAAPHMIMLGGKAVGDLSPNPRRAAGLGLVPEERLGRGAVPTLSLTANMLLSH 351 AL G+ + I + G RR L PEE L + +S+ NM L + Sbjct: 306 ALLGQRRLLLGE-IRVEGAPYAATREEMRRLRVFAL-PEEPLRNACIAGMSVAENMALRN 363 Query: 352 QKAPYVKRG--MIDFGFTRRAAASIIERFKVKAAGPDAPAKSLSGGNLQKFIVGREM--- 406 KRG ID +R A ++I F VK P+ P +LSGGN+Q+ ++ RE+ Sbjct: 364 FDVAPFKRGGWRIDRSAMKRQAQALISAFNVKPPVPERPIGTLSGGNVQRAVLARELGED 423 Query: 407 --ERKPAVFVVAQPTWGVDVGAAAQIHGEILKLKQEGCAVLVISEELDELFALCDRLHVI 464 + V +VA P +G+D + A IH +L+ + G AVL++SE+LDEL L DR+ V+ Sbjct: 424 GQDGAANVLIVANPVFGLDFASVADIHARLLQARARGAAVLLVSEDLDELLELSDRILVM 483 Query: 465 AKGRL---SPSTPIEQATREQVGVWMSG 489 +GR+ + E A R +G WM+G Sbjct: 484 TEGRIVHVAQDVAKEGADRAALGRWMAG 511 >gi|300310371|ref|YP_003774463.1| sugar ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 518 Score = 248 bits (633), Expect = 7e-67, Method: Compositional matrix adjust. Identities = 182/519 (35%), Positives = 280/519 (53%), Gaps = 41/519 (7%) Query: 1 VRKAYPGVLANDDVSLAVAPGEIHAVLGENGAGKSTLMKIIFGAVKPD---AGEIRWNGE 57 + K++PGV A ++V+LAV GEIHA++GENGAGKSTLMK++ G V P +G+I + GE Sbjct: 10 IEKSFPGVKALNNVNLAVREGEIHAIVGENGAGKSTLMKVLSG-VYPHGSYSGDIVYKGE 68 Query: 58 AVQIANPQVARKLGIAMVFQHFSLFDTLTVTENIALGLD-AKTDLKELAERITRTGE--- 113 + + + LGI ++ Q +L L+V EN+ LG + A+ + + + R E Sbjct: 69 VRAFKDIRDSEHLGIIIIHQELALVPLLSVMENLFLGNEQARGGVIDWEQSYVRAKELLA 128 Query: 114 KYGLELEPQRHVHTLSVGERQRVEIVRALLTEPQLLILDEPTSVLTPQAVEKLFVTLRRL 173 K GL+ P V L VG++Q +EI +AL E +LLILDEPT+ L + L L L Sbjct: 129 KVGLKESPLTQVGDLGVGKQQLIEIAKALSKEVKLLILDEPTASLNESDSDALLELLLEL 188 Query: 174 ADEGCSILYISHKLDEIRALCHSATVMRGGRVTGNCDPRNE--SSASLSRMMIGDELVRL 231 +G + + ISHKL+EI + S TV+R G D R E S + + M+G E+ Sbjct: 189 KRQGIASILISHKLNEISKVADSITVLRDGTTVDTFDCRAEPISEDRIIQHMVGREMADR 248 Query: 232 QRERNPE---------SMRNERKLHVNRLSLPKAHLFATELKDIECEVRAGEIVGIAGVS 282 +R+P+ R LH +RL++ KD+ VRAGEIVGIAG+ Sbjct: 249 YPQRDPQIGEVIFEVRDWRVHHPLHTDRLAI----------KDVNMSVRAGEIVGIAGLM 298 Query: 283 GNGQQELLAALSGEDQRAAPHMI----MLGGKAVGDLSPNPRR-AAGLGLVPEERLGRGA 337 G G+ EL ++ G RA I L GK V DLS + A G+ V E+R G G Sbjct: 299 GAGRTELAKSIFG---RAYGKKITGQAFLHGKEV-DLSTIEKAIAKGIAYVTEDRKGDGL 354 Query: 338 VPTLSLTANMLLSHQKAPYVKRGMIDFGFTRRAAASIIERFKVKAAGPDAPAKSLSGGNL 397 V + N+ L++ +R +ID + AA ++ +++ + +LSGGN Sbjct: 355 VLEEDIKKNISLANL-GGVSERTVIDEAREYKIAADFKQQMRIRCSSVLQKVVNLSGGNQ 413 Query: 398 QKFIVGREMERKPAVFVVAQPTWGVDVGAAAQIHGEILKLKQEGCAVLVISEELDELFAL 457 QK ++ + + +P V ++ +PT G+DVGA +I+ I KL EG +++IS E+ EL + Sbjct: 414 QKVVLSKWLFSQPEVLILDEPTRGIDVGAKFEIYNIISKLAAEGKCIIMISSEMPELLGM 473 Query: 458 CDRLHVIAKGRLSPSTPIEQATREQV--GVWMSGLWDEP 494 CDR++V+ +G+ P +AT+E + + + DEP Sbjct: 474 CDRIYVMNEGQFVGHLPKAEATQENIMRAIMRNKRIDEP 512 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,647,026 Number of extensions: 72373 Number of successful extensions: 851 Number of sequences better than 1.0e-08: 132 Number of HSP's gapped: 543 Number of HSP's successfully gapped: 176 Length of query: 506 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 417 Effective length of database: 1,198,455 Effective search space: 499755735 Effective search space used: 499755735 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)