BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0281.p (259 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313548|ref|YP_003777640.1| nitrate/sulfonate/bicarbonate A... 513 e-147 gi|300312968|ref|YP_003777060.1| nitrate/sulfonate/bicarbonate A... 108 4e-25 gi|300309803|ref|YP_003773895.1| nitrate/sulfonate/bicarbonate A... 107 1e-24 gi|300312684|ref|YP_003776776.1| nitrate/sulfonate/bicarbonate A... 96 4e-21 gi|300309716|ref|YP_003773808.1| nitrate/sulfonate/bicarbonate A... 94 2e-20 >gi|300313548|ref|YP_003777640.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 259 Score = 513 bits (1322), Expect = e-147, Method: Compositional matrix adjust. Identities = 259/259 (100%), Positives = 259/259 (100%) Query: 1 MKTPFQGKLNRAATTLAPWFLLLVILGVWQFICSVWQVSDFIFPSPGSILQAMVDYAGPI 60 MKTPFQGKLNRAATTLAPWFLLLVILGVWQFICSVWQVSDFIFPSPGSILQAMVDYAGPI Sbjct: 1 MKTPFQGKLNRAATTLAPWFLLLVILGVWQFICSVWQVSDFIFPSPGSILQAMVDYAGPI 60 Query: 61 TEHALSTFWVTMVGFAIAVVVGVTLGFVIGSSPMLYAAAYPLMTAFNALPKAAFVPVLVV 120 TEHALSTFWVTMVGFAIAVVVGVTLGFVIGSSPMLYAAAYPLMTAFNALPKAAFVPVLVV Sbjct: 61 TEHALSTFWVTMVGFAIAVVVGVTLGFVIGSSPMLYAAAYPLMTAFNALPKAAFVPVLVV 120 Query: 121 WFGIGAGPAILTAFLISFFPIMVNIATGLATLEPELEDVLRVLGARRMDILLKVGLPRSM 180 WFGIGAGPAILTAFLISFFPIMVNIATGLATLEPELEDVLRVLGARRMDILLKVGLPRSM Sbjct: 121 WFGIGAGPAILTAFLISFFPIMVNIATGLATLEPELEDVLRVLGARRMDILLKVGLPRSM 180 Query: 181 PYFFASLKVAITLAFVGSTVSEMNASNQGIGYLLVSAGSSMKMPLAFAGLVVIGAMAMAM 240 PYFFASLKVAITLAFVGSTVSEMNASNQGIGYLLVSAGSSMKMPLAFAGLVVIGAMAMAM Sbjct: 181 PYFFASLKVAITLAFVGSTVSEMNASNQGIGYLLVSAGSSMKMPLAFAGLVVIGAMAMAM 240 Query: 241 YELFAIIEKRTTGWAHRGK 259 YELFAIIEKRTTGWAHRGK Sbjct: 241 YELFAIIEKRTTGWAHRGK 259 >gi|300312968|ref|YP_003777060.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 283 Score = 108 bits (270), Expect = 4e-25, Method: Compositional matrix adjust. Identities = 64/201 (31%), Positives = 103/201 (51%) Query: 54 VDYAGPITEHALSTFWVTMVGFAIAVVVGVTLGFVIGSSPMLYAAAYPLMTAFNALPKAA 113 + + I H L T T++ F I V G+ +G + SP A P + A N++P+ Sbjct: 73 LSFPAEIYSHLLITLTETVLAFVIGTVFGLAIGLWLALSPTASAILDPYVKAANSMPRVI 132 Query: 114 FVPVLVVWFGIGAGPAILTAFLISFFPIMVNIATGLATLEPELEDVLRVLGARRMDILLK 173 P+ +WFG+G + A + FF + N+ G+ + P + R+LGA +L Sbjct: 133 LAPIFAMWFGLGIWSKVALAVTLVFFIVFFNVYQGVKEVSPVVLANARMLGANARQLLRT 192 Query: 174 VGLPRSMPYFFASLKVAITLAFVGSTVSEMNASNQGIGYLLVSAGSSMKMPLAFAGLVVI 233 V LP + + F+SL +I LAFVG+ V E S +G+GYL++ A + + FAG+VV+ Sbjct: 193 VYLPSATSWVFSSLHTSIGLAFVGAVVGEYLGSARGVGYLILQAEGTFDINTVFAGIVVL 252 Query: 234 GAMAMAMYELFAIIEKRTTGW 254 A+ + +IEKR W Sbjct: 253 TIFALVLDLAVGVIEKRLMKW 273 >gi|300309803|ref|YP_003773895.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 267 Score = 107 bits (267), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 60/217 (27%), Positives = 114/217 (52%), Gaps = 4/217 (1%) Query: 17 APWFLLLVILGVWQFICSVWQVSDFIFPSPGSILQAMVDYAGPITEHALSTFWVTMVGFA 76 AP + + +L +WQ +C++ +V ++ P+P I++ + + L T +T + FA Sbjct: 19 APLIIGIALLLMWQIVCTLLEVPPYLVPTPALIVKTLAADWSMLMASLLVTLKITFLAFA 78 Query: 77 IAVVVGVTLGFVIGSSPMLYAAAYPLMTAFNALPKAAFVPVLVVWFGIGAGPAILTAFLI 136 +AVV+GV + FV S + + +P P A P++++W + A ++ Sbjct: 79 LAVVLGVAIAFVFVQSRFIEISLFPYAIILQVTPIVAIAPLIIIWVKDTTAALTVCATIM 138 Query: 137 SFFPIMVNIATGLATLEPELEDVLRVLGARRMDILLKVGLPRSMPYFFASLKVAITLAFV 196 + FPI+ N GL ++ P L ++ R+ A R L ++ +P ++PYFF L+++ LA + Sbjct: 139 ALFPIISNTTLGLRSVNPGLLNLFRLNKATRWQTLRRLRIPSALPYFFGGLRISSGLALI 198 Query: 197 GSTVSEMNA----SNQGIGYLLVSAGSSMKMPLAFAG 229 G+ V+E A + G+ Y ++ AG + +P FA Sbjct: 199 GAVVAEFVAGTGGTGSGLAYQILQAGFQLNIPRLFAA 235 >gi|300312684|ref|YP_003776776.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 284 Score = 95.5 bits (236), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 66/240 (27%), Positives = 116/240 (48%), Gaps = 6/240 (2%) Query: 21 LLLVILGVWQFICSVWQVSDFIFPSPGSILQAMVDY------AGPITEHALSTFWVTMVG 74 +L+++LG W+ + + F F P I+ + D+ GP+ L T T++G Sbjct: 40 ILVIVLGGWEVCARIGWIDPFFFSQPSLIVAQIYDWMVEGTSQGPLWTQVLVTLEETVLG 99 Query: 75 FAIAVVVGVTLGFVIGSSPMLYAAAYPLMTAFNALPKAAFVPVLVVWFGIGAGPAILTAF 134 F I V GV G V+G + +L + N++P+ + V+ FG+G + A Sbjct: 100 FLIGSVAGVIAGIVLGRNKLLSDVFSLYIQIANSIPRVVLGSIFVIAFGLGMASKVALAV 159 Query: 135 LISFFPIMVNIATGLATLEPELEDVLRVLGARRMDILLKVGLPRSMPYFFASLKVAITLA 194 ++ FF + N G+ + + ++LGA R + + V +P ++ + ASL V+ A Sbjct: 160 VMVFFVVFANAFQGVREADRYMIANAQILGASRRQVTMAVVIPSALSWILASLHVSFGFA 219 Query: 195 FVGSTVSEMNASNQGIGYLLVSAGSSMKMPLAFAGLVVIGAMAMAMYELFAIIEKRTTGW 254 VG+ V E S QGIG L+ +A + FA ++V+ +A+A L +E+R W Sbjct: 220 LVGAVVGEFLGSKQGIGLLISTAQGAFNASGVFAAMIVLAVVALAADWLLHALERRLLKW 279 >gi|300309716|ref|YP_003773808.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 273 Score = 93.6 bits (231), Expect = 2e-20, Method: Compositional matrix adjust. Identities = 64/255 (25%), Positives = 126/255 (49%), Gaps = 20/255 (7%) Query: 23 LVILGVWQFICSVWQVSDFIFPSPGSILQAMVDYAGPITEH------------------- 63 ++++ +W+ ++ I PSP ++L+ V+YA P+ + Sbjct: 17 IIVIALWEGASRAGWINPQILPSPWAVLRKWVEYATPLKPYDPEAGSWLAWAFSGELVMD 76 Query: 64 ALSTFWVTMVGFAIAVVVGVTLGFVIGSSPMLYAAAYPLMTAFNALPKAAFVPVLVVWFG 123 A+ + + + GF I + + LG ++GSS +Y + +P A++P+ ++WFG Sbjct: 77 AIGSLYRVVAGFLIGAGLALPLGLLMGSSQRIYGLMNLTVQVIRPIPPIAYIPLAILWFG 136 Query: 124 IGAGPAILTAFLISFFPIMVNIATGLATLEPELEDVLRVLGARRMDILLKVGLPRSMPYF 183 +G PA+ L +FFP+++N G+ ++ R LGA + + L+V LP ++PY Sbjct: 137 LGNPPALFLISLGAFFPVLMNTIAGVRQVDSIYLRAARNLGASQSTLFLRVMLPAAVPYI 196 Query: 184 FASLKVAITLAFVGSTVSEMNASNQGIGYLLVSAGSSMKMPLAFAGLVVIGAMAMAMYEL 243 + +++ I AF+ V+EM A + G+G+ ++ A AG++ IG + +A+ Sbjct: 197 LSGVRIGIGTAFIVVIVAEMIAVSNGLGFRIMEAREYFWSDKIIAGMITIGLLGLAIDLG 256 Query: 244 FAIIEKRTTGWAHRG 258 + + W HRG Sbjct: 257 MSRLNNYMLRW-HRG 270 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.328 0.140 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 807,723 Number of extensions: 33523 Number of successful extensions: 205 Number of sequences better than 1.0e-08: 16 Number of HSP's gapped: 197 Number of HSP's successfully gapped: 16 Length of query: 259 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 176 Effective length of database: 1,226,865 Effective search space: 215928240 Effective search space used: 215928240 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 129 (54.3 bits)