BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0280.p (261 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313547|ref|YP_003777639.1| nitrate/sulfonate/bicarbonate A... 538 e-154 gi|300312621|ref|YP_003776713.1| ABC transporter ATPase [Herbasp... 184 7e-48 gi|300309802|ref|YP_003773894.1| nitrate/sulfonate/bicarbonate A... 177 7e-46 gi|300311645|ref|YP_003775737.1| taurine ABC transporter ATPase ... 173 1e-44 gi|300312685|ref|YP_003776777.1| nitrate/sulfonate/bicarbonate A... 172 3e-44 >gi|300313547|ref|YP_003777639.1| nitrate/sulfonate/bicarbonate ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 261 Score = 538 bits (1385), Expect = e-154, Method: Compositional matrix adjust. Identities = 260/261 (99%), Positives = 260/261 (99%) Query: 1 MSEPFVDFRHVYLAYDGAAEFAVEDIDLQTRAGEFIAIVGPSGCGKSTFMKLATGLKQPN 60 MSEPFVDFRHVYLAYDGAAEFAVEDIDLQTRAGEFIAIVGPSGCGKSTFMKLATGLKQPN Sbjct: 1 MSEPFVDFRHVYLAYDGAAEFAVEDIDLQTRAGEFIAIVGPSGCGKSTFMKLATGLKQPN 60 Query: 61 RGSVVIDGQTVTGPLKFVGMAFQAPTLLPWRTTLDNVLLPLEIVEPYRRDFKQKKAEYTE 120 RGSVVIDGQTVTGPLKFVGMAFQAPTLLPWRTTLDNVLLPLEIVEPYRRDFKQKKAEYTE Sbjct: 61 RGSVVIDGQTVTGPLKFVGMAFQAPTLLPWRTTLDNVLLPLEIVEPYRRDFKQKKAEYTE 120 Query: 121 RAKSLLKTVGLDGYADKFPWQLSGGMQQRASICRALIHEPKMLLLDEPFGALDAFTREEL 180 RA SLLKTVGLDGYADKFPWQLSGGMQQRASICRALIHEPKMLLLDEPFGALDAFTREEL Sbjct: 121 RALSLLKTVGLDGYADKFPWQLSGGMQQRASICRALIHEPKMLLLDEPFGALDAFTREEL 180 Query: 181 WCVLRDLWAERRFNVILVTHDLREAAFLADTIYVMSKRPGRIVVRRENPLPRPCPLETTY 240 WCVLRDLWAERRFNVILVTHDLREAAFLADTIYVMSKRPGRIVVRRENPLPRPCPLETTY Sbjct: 181 WCVLRDLWAERRFNVILVTHDLREAAFLADTIYVMSKRPGRIVVRRENPLPRPCPLETTY 240 Query: 241 TDGFNALVHELREHIGHVRNT 261 TDGFNALVHELREHIGHVRNT Sbjct: 241 TDGFNALVHELREHIGHVRNT 261 >gi|300312621|ref|YP_003776713.1| ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 257 Score = 184 bits (467), Expect = 7e-48, Method: Compositional matrix adjust. Identities = 97/201 (48%), Positives = 130/201 (64%), Gaps = 7/201 (3%) Query: 23 VEDIDLQTRAGEFIAIVGPSGCGKSTFMKLATGLKQPNRGSVVIDGQTVTGPLKFVGMAF 82 +E+ + +GE +A+VGPSGCGKST + +A GL++P+ GSV DGQT+ GP + F Sbjct: 18 IEEFTREIASGELVALVGPSGCGKSTLLHMAAGLEKPSSGSVAGDGQTIRGPHPERTLMF 77 Query: 83 QAPTLLPWRTTLDNVLLPLEIVEPYRRDFKQKKAEYTERAKSLLKTVGLDGYADKFPWQL 142 Q L PW T NV L LE + +KKA E+A+ L+ V L G+ +P Q+ Sbjct: 78 QENALYPWLTLQQNVALALEF-----QSVDKKKA--AEQARVWLENVKLKGFEHYYPHQV 130 Query: 143 SGGMQQRASICRALIHEPKMLLLDEPFGALDAFTREELWCVLRDLWAERRFNVILVTHDL 202 SGGM+QRA++ RA I +PK LLLDEPFGALDA TR L LRDL ++ V+LVTHD+ Sbjct: 131 SGGMRQRAALARAFISQPKALLLDEPFGALDALTRMTLQDALRDLIRQQGPTVVLVTHDV 190 Query: 203 REAAFLADTIYVMSKRPGRIV 223 EA FLAD I+V S RP +++ Sbjct: 191 DEALFLADRIFVFSPRPAKVL 211 >gi|300309802|ref|YP_003773894.1| nitrate/sulfonate/bicarbonate ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 278 Score = 177 bits (449), Expect = 7e-46, Method: Compositional matrix adjust. Identities = 95/216 (43%), Positives = 133/216 (61%), Gaps = 12/216 (5%) Query: 22 AVEDIDLQTRAGEFIAIVGPSGCGKSTFMKLATGLKQPNRGSVVIDG---QTVTGPLKFV 78 A++++ L GEF++++GPSGCGKST +K+ GL++P+ G + G TV P + + Sbjct: 35 ALDEVRLDIGRGEFVSLLGPSGCGKSTLLKMFAGLEEPSHGHIRWWGGNLATVGTPERTL 94 Query: 79 GMAFQAPTLLPWRTTLDNVLLPLEIVEPYRRDFKQKKAEYTERAKSLLKTVGLDGYADKF 138 M FQ TL+PW T NV LPL++ R A+ R + LK VGL+ + Sbjct: 95 AMVFQEATLMPWATVEQNVRLPLDLRHVPR-------AQADARVAASLKRVGLESFHAVL 147 Query: 139 PWQLSGGMQQRASICRALIHEPKMLLLDEPFGALDAFTREELWCVLRDLWAERRFNVILV 198 P +LSGGMQ RASI RAL P +LL+DEPFGALD FTR +L LRD+W+ + V+ V Sbjct: 148 PRELSGGMQMRASIARALATSPNLLLMDEPFGALDEFTRNKLDADLRDIWSRQDLTVVFV 207 Query: 199 THDLREAAFLADTIYVMSKRPGRIV--VRRENPLPR 232 TH + EA FL+ + VM+ RPGR++ V + P PR Sbjct: 208 THSIYEAVFLSSRVIVMAARPGRVIADVAIDAPGPR 243 >gi|300311645|ref|YP_003775737.1| taurine ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 261 Score = 173 bits (439), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 98/238 (41%), Positives = 133/238 (55%), Gaps = 14/238 (5%) Query: 22 AVEDIDLQTRAGEFIAIVGPSGCGKSTFMKLATGLKQPNRGSVVIDGQTVTGPLKFVGMA 81 A++ + L G F+ +G SGCGKST + G + G + +DGQ V+GP G+ Sbjct: 21 ALDRVSLDIPNGGFVVALGTSGCGKSTLLNAMAGFLPLSEGRISLDGQPVSGPGAERGVV 80 Query: 82 FQAPTLLPWRTTLDNVLLPLEIVEPYRRDFKQKKAEYTERAKSLLKTVGLDGYADKFPWQ 141 FQ +LLPW++ DNV L L+ + D + ERA+ LL+ VGL +A P++ Sbjct: 81 FQKDSLLPWKSVQDNVALGLKFAGVSKSDSR-------ERAQELLRLVGLQDFARAAPYE 133 Query: 142 LSGGMQQRASICRALIHEPKMLLLDEPFGALDAFTREELWCVLRDLWAERRFNVILVTHD 201 LSGGM+QR + RAL PK+LL+DEPFGALD+ TRE++ +L +WA V +TH Sbjct: 134 LSGGMRQRVGLARALAPNPKILLMDEPFGALDSMTREQMQELLVSVWARTGKQVFFITHS 193 Query: 202 LREAAFLADTIYVMSKRPGRIVVRRENPLPR----PCPLETTYTDGFNALVHELREHI 255 + EA FL + VMS RPGRIV R E R C T+ A ELRE I Sbjct: 194 IEEALFLGTEVIVMSPRPGRIVARFETDFVRRFAAGCEARAIKTEPAFA---ELREEI 248 >gi|300312685|ref|YP_003776777.1| nitrate/sulfonate/bicarbonate ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 275 Score = 172 bits (436), Expect = 3e-44, Method: Compositional matrix adjust. Identities = 95/237 (40%), Positives = 135/237 (56%), Gaps = 10/237 (4%) Query: 4 PFVDFRHV---YLAYDGAAEFAVEDIDLQTRAGEFIAIVGPSGCGKSTFMKLATGLKQPN 60 P ++ R+V ++ DG A A+ D + GEF+A+VGP+GCGKST + L TGL +P Sbjct: 20 PAIELRNVSCRFITADGRATVALRDFSMSVARGEFVAVVGPTGCGKSTTLSLITGLLKPT 79 Query: 61 RGSVVIDGQTVTGPLKFVGMAFQAPTLLPWRTTLDNVLLPLEIVEPYRRDFKQKKAEYTE 120 G V + GQ V G +G FQ + PWR+ +NV P R Q KA+ Sbjct: 80 LGEVRVMGQPVNGIDPRIGFVFQNDAVFPWRSVRENV-----AAGPLFRG--QPKAQAHA 132 Query: 121 RAKSLLKTVGLDGYADKFPWQLSGGMQQRASICRALIHEPKMLLLDEPFGALDAFTREEL 180 A ++ VGLD + D +P QLSGGM++R ++ + I+ P++LL+DEPF ALD TR + Sbjct: 133 LADEWIRRVGLDKFGDHYPHQLSGGMRKRVALAQTFINSPEILLMDEPFSALDMQTRTLM 192 Query: 181 WCVLRDLWAERRFNVILVTHDLREAAFLADTIYVMSKRPGRIVVRRENPLPRPCPLE 237 L LW+ +V+ VTHDL EA LAD +YV++ RP + LPRP +E Sbjct: 193 QDELLRLWSAHSGSVVFVTHDLEEAIALADKVYVLTARPATLKSVYPIDLPRPRVME 249 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.140 0.430 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 839,384 Number of extensions: 34478 Number of successful extensions: 461 Number of sequences better than 1.0e-08: 121 Number of HSP's gapped: 287 Number of HSP's successfully gapped: 133 Length of query: 261 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 178 Effective length of database: 1,226,865 Effective search space: 218381970 Effective search space used: 218381970 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 129 (54.3 bits)