BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0277.p (360 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313545|ref|YP_003777637.1| porin precursor transmembrane p... 722 0.0 gi|300311557|ref|YP_003775649.1| outer membrane porin [Herbaspir... 127 1e-30 gi|300309527|ref|YP_003773619.1| outer membrane protein (porin) ... 125 6e-30 gi|300313580|ref|YP_003777672.1| outer membrane porin [Herbaspir... 117 2e-27 gi|300310328|ref|YP_003774420.1| porin signal peptide protein [H... 112 5e-26 >gi|300313545|ref|YP_003777637.1| porin precursor transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 360 Score = 722 bits (1863), Expect = 0.0, Method: Compositional matrix adjust. Identities = 358/360 (99%), Positives = 359/360 (99%) Query: 1 MKKTRLALALASCMVAGSAMAQSSVTVSGLVDAYVGSMKNSGDTGSKATVGSGGMTTSWI 60 MKKTRLALALASCMVAGSAMAQSSVTVSGLVDAYVGSMKNSGDTGSKATVGSGGMTTSWI Sbjct: 1 MKKTRLALALASCMVAGSAMAQSSVTVSGLVDAYVGSMKNSGDTGSKATVGSGGMTTSWI 60 Query: 61 GFKGIEDLGGGLRAEFALTSFFQADTGAYGRFGGDNLFSRDANVGLAGDFGRVSLGRGLA 120 GFKGIEDLGGGLRAEFALTSFFQADTGAYGRFGGDNLFSRDANVGLAGDFGRVSLGRGLA Sbjct: 61 GFKGIEDLGGGLRAEFALTSFFQADTGAYGRFGGDNLFSRDANVGLAGDFGRVSLGRGLA 120 Query: 121 PSFLPTVLFNPFGDSFTFSPLVLHVYTPSGNFGSRTWTQANAADSGWSNEVIYTTPKFGG 180 PSFLPTVLFNPFGDSFTFSPLVLHVYTPSGNFGSRTWTQANAADSGWSN+VIYTTPKFGG Sbjct: 121 PSFLPTVLFNPFGDSFTFSPLVLHVYTPSGNFGSRTWTQANAADSGWSNQVIYTTPKFGG 180 Query: 181 LQANVHYQFGEVAGQSGKKNIGVNALYFNGGLALTALYFDAQVSNPNSGAAIIDATSPFS 240 LQANVHYQFGEVAGQSGKKNIGVNALYFNGGLALT LYFDAQVSNPNSGAAIIDATSPFS Sbjct: 181 LQANVHYQFGEVAGQSGKKNIGVNALYFNGGLALTGLYFDAQVSNPNSGAAIIDATSPFS 240 Query: 241 SITRQKTWMLGATYDFQVVKLYGTYQNSKDTTAGNPQAKDKLFSLGLSAPVGNGSILAGF 300 SITRQKTWMLGATYDFQVVKLYGTYQNSKDTTAGNPQAKDKLFSLGLSAPVGNGSILAGF Sbjct: 241 SITRQKTWMLGATYDFQVVKLYGTYQNSKDTTAGNPQAKDKLFSLGLSAPVGNGSILAGF 300 Query: 301 ARTKRTGDLLAGGDVSRNTTSVGYDYFMSKRTDLYAIVMNDKISTQSNAVSFGLGMRHRF 360 ARTKRTGDLLAGGDVSRNTTSVGYDYFMSKRTDLYAIVMNDKISTQSNAVSFGLGMRHRF Sbjct: 301 ARTKRTGDLLAGGDVSRNTTSVGYDYFMSKRTDLYAIVMNDKISTQSNAVSFGLGMRHRF 360 >gi|300311557|ref|YP_003775649.1| outer membrane porin [Herbaspirillum seropedicae SmR1] Length = 374 Score = 127 bits (319), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 132/405 (32%), Positives = 184/405 (45%), Gaps = 76/405 (18%) Query: 1 MKKTRLALALASCMVAGSAMAQSSVTVSGLVDAYVGSMKNSGDTG-SKATVGSGGMTTSW 59 MKK+ L L A G+A AQS+VT+ G+VD + + N+ G S + +G + S Sbjct: 1 MKKSSLLLLAAGLTSGGAAFAQSNVTIYGIVDTSIHYLSNANAQGNSNVRMDNGAIANSR 60 Query: 60 IGFKGIEDLGGGLRAEFALTSFFQADTGAYGRFGGDNLFSRDANVGLAGDFGRVSLGRGL 119 IGFKG EDLGGGL+A F L + F +DTGA + G +LF R + VGL G FG+V +GR Sbjct: 61 IGFKGTEDLGGGLKAMFQLENGFSSDTGAMQQ--GGSLFGRQSWVGLQGGFGKVRVGRQN 118 Query: 120 APSFLPTVLFNPFGDSFTFSPLVLHVYTPSGNFGSRTWTQANAADSGWSNEVIYTTPKFG 179 P LF+ D F PL + GN+ S +W A A+ N + Y FG Sbjct: 119 TP------LFDLLADH--FDPLTV------GNYASNSWLPAAASLVRSPNMLRY-DGDFG 163 Query: 180 GLQANVHYQFGEVAGQSGKKNIGVNALYFNGG-LALTALYFDAQVSNPNSGAAIIDATSP 238 + A+ + FGE AG + +AL + G LAL G S Sbjct: 164 PVAASASWAFGEKAGSVRTGSQFSSALRYTAGPLAL--------------GGGYQQTVSS 209 Query: 239 FSSITRQKTWMLGATYDFQVVKLYGTYQNSKD-------------------TTAGNP--- 276 +S + TW L A+YDF V K++G + N KD TTAG Sbjct: 210 TNSSLKHTTWNLSASYDFGVAKVFGGWLNIKDDSGLTGSVMAADGDVSNGWTTAGAANNG 269 Query: 277 ----QAKDKLFSLGLSAPVGNGSILAG---FARTKRTGDLLAG--GDVSRNTTSVGYDYF 327 + KD F LG S N L G + R+K + G GD R T DY Sbjct: 270 STGVKRKDNGFFLGSSYKATNALTLTGAFYYDRSKNVSYVSTGNVGDGKRYTLVGWADYA 329 Query: 328 MSKRTDLYAIV------------MNDKISTQSNAVSFGLGMRHRF 360 +SKRT LY + + + + +++ + G+G+RH F Sbjct: 330 LSKRTQLYTTIDYNKAKDAAVYELTNSANGKNSLTTVGVGIRHIF 374 >gi|300309527|ref|YP_003773619.1| outer membrane protein (porin) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 390 Score = 125 bits (313), Expect = 6e-30, Method: Compositional matrix adjust. Identities = 137/411 (33%), Positives = 188/411 (45%), Gaps = 72/411 (17%) Query: 1 MKKTRLALALASCMVAGSAMAQSSVTVSGLVDAYVGSMKN----SGDTGSKATVGSGGMT 56 MKK+ LALA+ +A AQSSVT+ G+VD V +G TGSK ++ SG + Sbjct: 1 MKKSLLALAVLGAFAG-AAQAQSSVTIYGIVDTGVVYQSKVATPTGGTGSKFSLNSGVIQ 59 Query: 57 TSWIGFKGIEDLGGGLRAEFALTSFFQADTGAYGRFGGDN-LFSRDANVGLAGDFGRVSL 115 S IGFKG EDLGGGL A F L + F DTGA N LF R + VGLAG+FG V L Sbjct: 60 GSRIGFKGTEDLGGGLSAVFNLEAGFSNDTGALDDSKTSNTLFRRKSVVGLAGNFGSVLL 119 Query: 116 GRGLAPSFLPTVLFNPFGDSFTFSPLVLHVYTPSGNFGSRTWTQANAAD----SGWSNEV 171 GR F D+ + YT +FG T + D + +N + Sbjct: 120 GRQ-----------TDFADT-------ISSYTSVQDFGGWTGAAGHNLDRLEGTRTNNSI 161 Query: 172 IYTTPKFGGLQANVHYQFGEVAGQ-SGKKNIGVNALYFNGGLALTALYFDAQVSNPNSGA 230 YTT G N+ Y FGE AGQ S + GV Y NG L L Y+ ++ + + A Sbjct: 162 SYTTANLNGFTGNLMYGFGETAGQTSAGQAFGVGGKYDNGPLGLGINYYQSKAGSTPADA 221 Query: 231 AIIDA--TSPFSS-----ITRQKTWMLGATYDFQVVKLYGTYQNSKDTTAGNPQAKDKLF 283 +++ A T+ F++ T QK + A+Y F ++YG + K N QA Sbjct: 222 SLLAATGTNQFTNGTNNGSTAQKVLNVAASYQFGPARVYGNWSRVKQDL--NTQANLTAA 279 Query: 284 SLGLSAPVGNGSILA-GFARTKRTG---------DLLAGGDVSR------------NTTS 321 +G A + N ++ A A G LLA D +R S Sbjct: 280 GVGSLATISNRTLAASNKADIYEIGTAYSLSPSLKLLAAVDHTRAQFDGVSGKAKLTQVS 339 Query: 322 VGYDYFMSKRTDLYAIV------------MNDKISTQSNAVSFGLGMRHRF 360 +G DY++SKRTDLYA + + D T N + +G+RH+F Sbjct: 340 LGTDYWLSKRTDLYAFLAYSKATDLNNPGVADSSGTDGNQGAVAVGIRHKF 390 >gi|300313580|ref|YP_003777672.1| outer membrane porin [Herbaspirillum seropedicae SmR1] Length = 387 Score = 117 bits (292), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 137/414 (33%), Positives = 187/414 (45%), Gaps = 81/414 (19%) Query: 1 MKKTRLALALASCMVAGSAMAQSSVTVSGLVD---AY---VGSMKNSGD--TGSKATVGS 52 MKK+ LALA+ +A AQSSVT+ G+VD AY VG +G+ TGSK + S Sbjct: 1 MKKSLLALAVLGAFAG-AAQAQSSVTIYGIVDTGIAYSSKVGYTDANGNAATGSKFGINS 59 Query: 53 GGMTTSWIGFKGIEDLGGGLRAEFALTSFFQADTGAYGRFGGD-----NLFSRDANVGLA 107 G + S IGFKG+EDLGGGL A F L + F +D G G G D NLF R + VGL+ Sbjct: 60 GVIQGSRIGFKGVEDLGGGLSAVFNLETGFTSDDG--GLQGSDAVTSSNLFRRKSVVGLS 117 Query: 108 GDFGRVSLGRGLAPSFLPTVLFNPFGDSFTFSPLVLHVYTPSGNFGSRTWTQANAAD--- 164 G FG V LGR F D+ + YT +FG ++ + Sbjct: 118 GGFGTVLLGRQ-----------TDFADT-------ISAYTAVADFGGVVANSGSSLNRLQ 159 Query: 165 -SGWSNEVIYTTPKFGGLQANVHYQFGEVAGQ-SGKKNIGVNALYFNGGLALTALYFDAQ 222 + +N V YTT G N+ Y FGE AG+ S + G+ Y NG L L Y+ ++ Sbjct: 160 GTRTNNSVSYTTANLSGFTGNLIYGFGEQAGKTSAGQAYGIGGKYDNGPLGLGINYYQSK 219 Query: 223 VSNPNSGAAIIDATSPFSSI--------TRQKTWMLGATYDFQVVKLYGTYQNSKDTTAG 274 + S ++I A ++ T K+ + A+Y F ++Y Y K G Sbjct: 220 AGSTPSDTSLIPAAGATNANTALGVAGNTALKSLNVVASYQFGPARVYANYSRVKQDLKG 279 Query: 275 NPQAKDKLFSLGLSA---PVGNGSILAGFARTKRTGDLLAGGDVSRNT------------ 319 P + A +G L+ + LLA D SR T Sbjct: 280 TPTVATATLANAQKADTYEIGTAYSLSPSLK------LLAAVDHSRATFNGVNTKGKLTQ 333 Query: 320 TSVGYDYFMSKRTDLYAIVMNDKIS-TQSNAVSFG------------LGMRHRF 360 S+G DYF+SKRTDLYA V N + S Q+ V+ G G+RH+F Sbjct: 334 ISLGADYFLSKRTDLYAFVSNMRASDIQNPGVTGGATGSDASQTAIITGIRHKF 387 >gi|300310328|ref|YP_003774420.1| porin signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 405 Score = 112 bits (280), Expect = 5e-26, Method: Compositional matrix adjust. Identities = 97/287 (33%), Positives = 142/287 (49%), Gaps = 39/287 (13%) Query: 1 MKKTRLALALASCMVAGSAMAQSS-VTVSGLVDA---YVGSMKNSG-DTGSKATVGSGGM 55 MKK+ LALA+ AG+A AQSS +T+ G +DA Y + G +TGS V SG + Sbjct: 1 MKKSLLALAVLGAF-AGAAHAQSSSITIYGAIDASLTYTNKIATDGKNTGSLTAVESGLV 59 Query: 56 TTSWIGFKGIEDLGGGLRAEFALTSFFQADTGAYGRFGGDNLFSRDANVGLAGDFGRVSL 115 S +G KG EDLGGGL+A F L + F DTG G+ G ++ R VGL+G+FG V + Sbjct: 60 KGSRLGIKGSEDLGGGLKAVFTLENGFNVDTGEAGQ--GKLMWGRQTTVGLSGNFGTVLV 117 Query: 116 GRGLAPSFLPTVLFNPFGDSFTFSPLVLHVYTPSGNFGSRTWTQANAAD----SGWSNEV 171 GR P N G +G FG + D +N + Sbjct: 118 GRQNDP-------LNDIGG-----------IASAGGFGVPGTVHSKGLDRTDGQRLNNSI 159 Query: 172 IYTTPKFGGLQANVHYQFGEVAGQS-GKKNIGVNALYFNGGLALTALYFDAQVSNPNSGA 230 Y +P F GL + Y FGE G++ G ++IG+ + Y NG L+L YF +++ + G+ Sbjct: 160 RYNSPNFNGLSFSGIYGFGEQVGRAVGGRSIGLGSQYVNGPLSLGLGYFQSKMGGGSDGS 219 Query: 231 AIIDATSPFSSI--------TRQKTWMLGATYDFQVVKLYGTYQNSK 269 + ++A + + T KTW L A Y F K+YG + + + Sbjct: 220 SDVNAKAACNGAGEKDHAGDTCLKTWTLAAAYQFGPAKVYGAFSHVR 266 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.133 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,215,781 Number of extensions: 57196 Number of successful extensions: 225 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 173 Number of HSP's successfully gapped: 14 Length of query: 360 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 274 Effective length of database: 1,212,660 Effective search space: 332268840 Effective search space used: 332268840 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)