BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0270.p (215 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313540|ref|YP_003777632.1| transcription regulator protein... 443 e-126 gi|300313534|ref|YP_003777626.1| GntR family transcription regul... 117 1e-27 gi|300313824|ref|YP_003777916.1| GntR family transcription regul... 107 1e-24 gi|300309587|ref|YP_003773679.1| GntR family transcription regul... 66 2e-12 gi|300312446|ref|YP_003776538.1| GntR family transcription regul... 58 7e-10 >gi|300313540|ref|YP_003777632.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 245 Score = 443 bits (1139), Expect = e-126, Method: Compositional matrix adjust. Identities = 215/215 (100%), Positives = 215/215 (100%) Query: 1 MYQDIYDAIMEHRLPPRTKLTEQTLCQIYDTARHTVRKVLARLAAEGMVDLEANRGAFIA 60 MYQDIYDAIMEHRLPPRTKLTEQTLCQIYDTARHTVRKVLARLAAEGMVDLEANRGAFIA Sbjct: 31 MYQDIYDAIMEHRLPPRTKLTEQTLCQIYDTARHTVRKVLARLAAEGMVDLEANRGAFIA 90 Query: 61 SPSPAEVRDMFELRNILEHAVLEKLGHTAGTRQIAGLRRMVEEERDCYLNGDRPRWIRLS 120 SPSPAEVRDMFELRNILEHAVLEKLGHTAGTRQIAGLRRMVEEERDCYLNGDRPRWIRLS Sbjct: 91 SPSPAEVRDMFELRNILEHAVLEKLGHTAGTRQIAGLRRMVEEERDCYLNGDRPRWIRLS 150 Query: 121 AQFHLALAELTGNALLVETLRKLVSRTTLMIAKTEAPGHNACSFDEHETVLEALEKGDIK 180 AQFHLALAELTGNALLVETLRKLVSRTTLMIAKTEAPGHNACSFDEHETVLEALEKGDIK Sbjct: 151 AQFHLALAELTGNALLVETLRKLVSRTTLMIAKTEAPGHNACSFDEHETVLEALEKGDIK 210 Query: 181 AAQAHMAHHLHLCEDRVRPDADDQFDLRAVLGKGL 215 AAQAHMAHHLHLCEDRVRPDADDQFDLRAVLGKGL Sbjct: 211 AAQAHMAHHLHLCEDRVRPDADDQFDLRAVLGKGL 245 >gi|300313534|ref|YP_003777626.1| GntR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 249 Score = 117 bits (292), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 75/198 (37%), Positives = 107/198 (54%), Gaps = 13/198 (6%) Query: 5 IYDAIMEHRLPPRTKLTEQTLCQIYDTARHTVRKVLARLAAEGMVDLEANRGAFIASPSP 64 I +AI+ +L P T+L EQ L ++ +R VR+ L L A G+V + A RG F+ P+P Sbjct: 17 ITEAILAKQLAPGTRLGEQQLADLFKVSRTQVREALTHLMARGIVTVSARRGWFVIEPTP 76 Query: 65 AEVRDMFELRNILE-----HAVLEKLGHTAGTRQIAGLRRMVEEERDCYLNGDRPRWIRL 119 A+ R+ FE R ++E A L +G TA I LR + E D L Sbjct: 77 AQAREAFEARRVIELGLLRQARLRPVGDTA----IGQLREHIAREEAAIAGADVGARSYL 132 Query: 120 SAQFHLALAELTGNALLVETLRKLVSRTTL--MIAKTEAPGHNACSFDEHETVLEALEKG 177 FH+ LAE GN+LL ETLR L +RTTL M+ ++ ++CS EH ++EALE+G Sbjct: 133 LGDFHVCLAECLGNSLLAETLRDLTARTTLTAMLHQSSEQAEDSCS--EHVLIVEALERG 190 Query: 178 DIKAAQAHMAHHLHLCED 195 D++ A+ M HL E Sbjct: 191 DLEQAEQLMHDHLQHVES 208 >gi|300313824|ref|YP_003777916.1| GntR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 228 Score = 107 bits (266), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 63/190 (33%), Positives = 97/190 (51%) Query: 1 MYQDIYDAIMEHRLPPRTKLTEQTLCQIYDTARHTVRKVLARLAAEGMVDLEANRGAFIA 60 + DI I RLPP KL E+ L ++Y +R +R L LA + +VDL ++GAF++ Sbjct: 14 IASDIAANIAAQRLPPGAKLPEEALARVYQVSRTKIRAALVMLAKDKLVDLIPDKGAFVS 73 Query: 61 SPSPAEVRDMFELRNILEHAVLEKLGHTAGTRQIAGLRRMVEEERDCYLNGDRPRWIRLS 120 P+ E R++F +R LE A++ + A A L +E ER + + L Sbjct: 74 KPTVEETRNIFFVRRTLESAMVREFVARATPEDYARLEAHLELERQAVSDDNMQVRSTLL 133 Query: 121 AQFHLALAELTGNALLVETLRKLVSRTTLMIAKTEAPGHNACSFDEHETVLEALEKGDIK 180 + FH+ LA + GN +L + L KL R+ L+ ++ ACS +EH + A GD + Sbjct: 134 SDFHILLARIVGNDVLTDILEKLAGRSALITMLYQSTRDAACSSEEHRAFIAAARAGDTE 193 Query: 181 AAQAHMAHHL 190 A M HHL Sbjct: 194 RAMELMEHHL 203 >gi|300309587|ref|YP_003773679.1| GntR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 249 Score = 65.9 bits (159), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 62/217 (28%), Positives = 94/217 (43%), Gaps = 30/217 (13%) Query: 1 MYQDIYDAIMEHRLPPRTKLTEQTLCQIYDTARHTVRKVLARLAAEGMVDLEANRGAFIA 60 MYQ + + + RLPP L EQ +R ++R+ L L EG V++ G ++ Sbjct: 34 MYQPGFTS--QGRLPPERALAEQ-----LGVSRPSIREALVALELEGRVEIRMGSGVYLT 86 Query: 61 SPSPA--------------EVRDMFELRNILEHAVLEKLGHTAGTRQIAGLRRMVEE-ER 105 +P + D+ R I E A++ + A R I LR + + ER Sbjct: 87 QAAPPGQQEEPAPDKEMGNSLSDIMGARAIFEGAIVALVAPLAKPRDIKALRAIYDTMER 146 Query: 106 DCYLNGDRPRWIRLSAQFHLALAELTGNALLVETLRKLVS-RTTLMIAK----TEAPGHN 160 +C G P FH+ +A +TGN +LV+T+ +L R + AK E Sbjct: 147 ECQ-AGQSPAAT--DRDFHIRIARMTGNDVLVQTVARLYDERQDPISAKLLDHYEPEDSW 203 Query: 161 ACSFDEHETVLEALEKGDIKAAQAHMAHHLHLCEDRV 197 + EH +LEA+E D AQA M HL + R Sbjct: 204 LTALHEHREILEAIEARDAIQAQAAMQRHLKMSLKRT 240 >gi|300312446|ref|YP_003776538.1| GntR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 255 Score = 57.8 bits (138), Expect = 7e-10, Method: Compositional matrix adjust. Identities = 64/214 (29%), Positives = 97/214 (45%), Gaps = 28/214 (13%) Query: 9 IMEHRLPPRTKL-TEQTLCQIYDTARHTVRKVLARLAAEGMVD---------LEANRGAF 58 I L P KL TE + Q +R VR+ ++RL A G+V+ LEA +G Sbjct: 32 ISNGSLTPGDKLPTESEIMQTQGVSRTVVREAISRLQAAGLVETRHGIGTFVLEAKKGGN 91 Query: 59 IASPSPAEVRDMFELRNILEHAV---LEKLGHTAGTR---QIAGLRRMVEEERDCYLNGD 112 I + PA + M +L +LE + E G A R Q+ +R ++ R G Sbjct: 92 I-NIDPATITTMRDLLALLELRISLETETAGLAAARRTEEQLQEIREALDLFRANQQGGG 150 Query: 113 RPRWIRLSAQFHLALAELTGNALLVETLRKL----VSRTTLMIAKTEAPGHNACSFD--- 165 + +FH A+A TGN +E + L + R + A+ A A D Sbjct: 151 D--TVGPDFRFHFAIANATGNRYFIEIISHLGMGVIPRKRINTAEL-AHDQQASYLDRVQ 207 Query: 166 -EHETVLEALEKGDIKAAQAHMAHHLHLCEDRVR 198 EHE + EA+ +GD +AA+A M +HL +R+R Sbjct: 208 REHEDIYEAIARGDAEAARAAMRNHLTNSRERLR 241 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.135 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 610,279 Number of extensions: 21791 Number of successful extensions: 103 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 104 Number of HSP's successfully gapped: 11 Length of query: 215 Length of database: 1,619,870 Length adjustment: 81 Effective length of query: 134 Effective length of database: 1,236,335 Effective search space: 165668890 Effective search space used: 165668890 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 128 (53.9 bits)