BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0253.p (543 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313528|ref|YP_003777620.1| glycosyltransferase [Herbaspiri... 1118 0.0 gi|300312060|ref|YP_003776152.1| glycosyl transferase group 1 pr... 69 9e-13 gi|300312062|ref|YP_003776154.1| membrane-anchored group 1 glyco... 65 1e-11 >gi|300313528|ref|YP_003777620.1| glycosyltransferase [Herbaspirillum seropedicae SmR1] Length = 543 Score = 1118 bits (2893), Expect = 0.0, Method: Compositional matrix adjust. Identities = 540/543 (99%), Positives = 541/543 (99%) Query: 1 MSPVTSSSPAQADADRYQRRADSCDVMLLLEGTFPYVSGGVSSWVNQIIRGFPHLRFGLC 60 MSPVT SSPA ADADRYQRRADSCDVMLLLEGTFPYVSGGVSSWVNQIIRGFPHLRFGLC Sbjct: 1 MSPVTFSSPAPADADRYQRRADSCDVMLLLEGTFPYVSGGVSSWVNQIIRGFPHLRFGLC 60 Query: 61 FIGSRRQDYGAPKYALPDNVVHLETHYIHENLSATQVVRSRGDAQAFAMMDQLHGMLRAY 120 FIGSRRQDYGAPKYALPDNVVHLETHYIHENLSATQVVRSRGDAQAFAMMDQLHGMLRAY Sbjct: 61 FIGSRRQDYGAPKYALPDNVVHLETHYIHENLSATQVVRSRGDAQAFAMMDQLHGMLRAY 120 Query: 121 DRSDSTSHSALAAQLAELIPMMADGGKLDQDAFLFSKQSWDYLTAQYRTFCTDPSFVDYF 180 DRSDSTSHSALAAQLAELIPMMADGGKLDQDAFLFSKQSWDYLTAQYRTFCTDPSFVDYF Sbjct: 121 DRSDSTSHSALAAQLAELIPMMADGGKLDQDAFLFSKQSWDYLTAQYRTFCTDPSFVDYF 180 Query: 181 WTVRIMHQPVWTLARVARQLIPARCYHTVSTGYAGFLGSLMKMQTGRPLILSEHGIYTKE 240 WTVRIMHQPVWTLARVARQLIPARCYHTVSTGYAGFLGSLMKMQTGRPLILSEHGIYTKE Sbjct: 181 WTVRIMHQPVWTLARVARQLIPARCYHTVSTGYAGFLGSLMKMQTGRPLILSEHGIYTKE 240 Query: 241 RKIDLFQSEWIRDNRNVFEKDASQIPYFREMWIRFFEALGRMCYQASNQIVALYETNRLR 300 RKIDLFQSEWIRDNRNVFEKDASQIPYFREMWIRFFEALGRMCYQASNQIVALYETNRLR Sbjct: 241 RKIDLFQSEWIRDNRNVFEKDASQIPYFREMWIRFFEALGRMCYQASNQIVALYETNRLR 300 Query: 301 QIIDGAPPERTRNIPNGIDLPRLSALRAQRSEKIPPVLCLLGRVVPIKDVKTFIRAMRTV 360 Q+IDGAPPERTRNIPNGIDLPRLSALRAQRSEKIPPVLCLLGRVVPIKDVKTFIRAMRTV Sbjct: 301 QVIDGAPPERTRNIPNGIDLPRLSALRAQRSEKIPPVLCLLGRVVPIKDVKTFIRAMRTV 360 Query: 361 VNQYPEAEAWIAGPEDEDPSYAAECHAIAEGLGLLDKVKFLGYQKIDELMPKIGLVVLSS 420 VNQYPEAEAWIAGPEDEDPSYAAECHAIAEGLGLLDKVKFLGYQKIDELMPKIGLVVLSS Sbjct: 361 VNQYPEAEAWIAGPEDEDPSYAAECHAIAEGLGLLDKVKFLGYQKIDELMPKIGLVVLSS 420 Query: 421 ISEALPLVLLEGFAAGVPGVATDVGSCRQLIYGLSEEDQALGAAGDVVGIAEPQALAQAA 480 ISEALPLVLLEGFAAGVPGVATDVGSCRQLIYGLSEEDQALGAAGDVVGIAEPQALAQAA Sbjct: 421 ISEALPLVLLEGFAAGVPGVATDVGSCRQLIYGLSEEDQALGAAGDVVGIAEPQALAQAA 480 Query: 481 LKLLTDPQRWYAAQAAGIARVERYYTQEMMFGAYRELYDNALAEQGAAATGAAATALRCP 540 LKLLTDPQRWYAAQAAGIARVERYYTQEMMFGAYRELYDNALAEQGAAATGAAATALRCP Sbjct: 481 LKLLTDPQRWYAAQAAGIARVERYYTQEMMFGAYRELYDNALAEQGAAATGAAATALRCP 540 Query: 541 VQH 543 VQH Sbjct: 541 VQH 543 >gi|300312060|ref|YP_003776152.1| glycosyl transferase group 1 protein [Herbaspirillum seropedicae SmR1] Length = 388 Score = 68.9 bits (167), Expect = 9e-13, Method: Compositional matrix adjust. Identities = 78/295 (26%), Positives = 129/295 (43%), Gaps = 34/295 (11%) Query: 247 QSEWIRDNRNVFEKDASQIPYFREMWIRFFEALGRMCYQASNQIVALYETNRLRQIIDGA 306 E RD R+ ++A R + + F++ CY A V+ + LR I Sbjct: 113 HGEHGRDARDPHGRNARH-NLLRRLVLPFYD-----CYYA----VSADLRDWLRDTIK-V 161 Query: 307 PPERTRNIPNGIDLPRLSALRAQRSEKIP--PVLCLL----GRVVPIKDVKTFIRAMRTV 360 P ++ + NG+D R + E P P C++ GRV +KD T + A + Sbjct: 162 PADKNLLLANGVDTARFHPPADGQREATPELPAGCMVFGAVGRVQDVKDHATLVNAFIAL 221 Query: 361 VNQYPEAEA----WIAGPEDEDPSYAAECHAIAEGLGLLDKVKFLGYQ-KIDELMPKIGL 415 + P+ A I G P+ A+ A G+ D V G + ++ +M + + Sbjct: 222 LAMRPQQRARLRLAIVGEGPLLPALRAQVQAA----GIADLVWLPGARMDVEVMMRRFSV 277 Query: 416 VVLSSISEALPLVLLEGFAAGVPGVATDVGSCRQLIYGLSEEDQALGAAGDVVGIAEPQA 475 +LSSI+E +P+ +LE A G+P ++T VG +++ GA G + + A Sbjct: 278 FMLSSIAEGVPVTILEAMATGLPVISTAVGGVPEVV--------TEGATGQLTPAGDAAA 329 Query: 476 LAQAALKLLTDPQRWYAAQAAGIARVERYYTQEMMFGAYRELYDNALAEQGAAAT 530 LA+A + DP AAG+ R R ++ M AY +YD LA + +T Sbjct: 330 LARAMAAYVDDPALAARHGAAGLERCRRQHSMAAMLQAYAGMYDRMLAGKRGQST 384 >gi|300312062|ref|YP_003776154.1| membrane-anchored group 1 glycosyltransferase [Herbaspirillum seropedicae SmR1] Length = 406 Score = 65.1 bits (157), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 69/268 (25%), Positives = 112/268 (41%), Gaps = 33/268 (12%) Query: 274 RFFEALGRMCYQASNQIVALYETNRLRQIIDGAPPERTRNIPNGIDLPRLS-------AL 326 R AL + ++ + + E R G P + IPN +++ S AL Sbjct: 148 RLTRALETYALKRADAVTTICEGLRADIAARGVPLAKMTVIPNAVNIDDFSLGQQADPAL 207 Query: 327 RAQRSEKIPPVLCLLGRVVPIKDVKTFIRAMRTVVNQYPEAEAWIAGPEDEDPSYAAECH 386 Q + +L +G + + ++A+ ++ P+ + G ++ + H Sbjct: 208 ARQLGLEGKTLLGFIGSFYAYEGLPVLVQALPQLLAGNPDVRLLLVGGGPQERA----LH 263 Query: 387 AIAEGLGLLDKVKFLG----------YQKIDELM-PKIGLVVLSSISEALPLVLLEGFAA 435 A+A LG+ DKV F G Y ID L+ P++ + + ++ PL LE A Sbjct: 264 ALAAELGVSDKVVFAGRVPHEQVQRYYNLIDVLVYPRLKMRLTDLVT---PLKPLEAMAQ 320 Query: 436 GVPGVATDVGSCRQLIYGLSEEDQALGAAGDVVGIAEPQALAQAALKLLTDPQRWYAAQA 495 G VA+DVG R+LI G G + P+ALAQ L LL +P RW +A Sbjct: 321 GRLVVASDVGGHRELISD--------GRTGVLFAAGSPEALAQKVLALLAEPARWPQLRA 372 Query: 496 AGIARVERYYTQEMMFGAYRELYDNALA 523 AG VE Y+++Y +A Sbjct: 373 AGRHFVETERNWAASVARYQQIYPRLVA 400 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.136 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,619,171 Number of extensions: 66747 Number of successful extensions: 204 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 199 Number of HSP's successfully gapped: 4 Length of query: 543 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 453 Effective length of database: 1,193,720 Effective search space: 540755160 Effective search space used: 540755160 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)