BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0246.p (292 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313519|ref|YP_003777611.1| 6-phosphogluconate dehydrogenas... 580 e-167 gi|300312370|ref|YP_003776462.1| 3-hydroxyisobutyrate dehydrogen... 158 4e-40 gi|300310292|ref|YP_003774384.1| 3-hydroxyisobutyrate dehydrogen... 108 8e-25 gi|300311332|ref|YP_003775424.1| dehydrogenase [Herbaspirillum s... 74 1e-14 >gi|300313519|ref|YP_003777611.1| 6-phosphogluconate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 298 Score = 580 bits (1495), Expect = e-167, Method: Compositional matrix adjust. Identities = 290/292 (99%), Positives = 291/292 (99%) Query: 1 VIGLGAMGYGVASSLLRAGFNVHACDVRKEVLDKFAAAGGVACANPAELAGKVDVVITLV 60 VIGLGAMGYGVASSLLRAGFNVHACDVRKEVLDKFAAAGGVACANPAELAGKVDVVITLV Sbjct: 7 VIGLGAMGYGVASSLLRAGFNVHACDVRKEVLDKFAAAGGVACANPAELAGKVDVVITLV 66 Query: 61 VNAAQTETVLFGDNGAVSAMKPGSVVISSATVAPDFAIELGKRLAEKGLLLLDAPVSGGA 120 VNAAQTETVLFGDNGAVSAMKPGSVVISSATVAPDFAIELGKRL EKGLLLLDAPVSGGA Sbjct: 67 VNAAQTETVLFGDNGAVSAMKPGSVVISSATVAPDFAIELGKRLVEKGLLLLDAPVSGGA 126 Query: 121 ARAASGEMTMMTSGPAEVYAKIEDVLAGMAGKVYRLGDTHGIGSKVKIINQLLAGVHIAA 180 ARAASGEMTMMTSGPAEVYAKIEDVLAGMAGKVYRLGDTHGIGSKVKIINQLLAGVHIAA Sbjct: 127 ARAASGEMTMMTSGPAEVYAKIEDVLAGMAGKVYRLGDTHGIGSKVKIINQLLAGVHIAA 186 Query: 181 SAEAMALGLREGVNPDALYEVITHSAGNSWMFENRVPHILNGDYTPLSAVDIFVKDLGLV 240 SAEAMALGLREGVNPDALYEVITHSAGNSWMFENRVPHIL+GDYTPLSAVDIFVKDLGLV Sbjct: 187 SAEAMALGLREGVNPDALYEVITHSAGNSWMFENRVPHILSGDYTPLSAVDIFVKDLGLV 246 Query: 241 LDTARRSKFPLPLSAAAHQMFMMASTAGHGGEDDSAVIKIFPGIDLPPAKAK 292 LDTARRSKFPLPLSAAAHQMFMMASTAGHGGEDDSAVIKIFPGIDLPPAKAK Sbjct: 247 LDTARRSKFPLPLSAAAHQMFMMASTAGHGGEDDSAVIKIFPGIDLPPAKAK 298 >gi|300312370|ref|YP_003776462.1| 3-hydroxyisobutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 297 Score = 158 bits (400), Expect = 4e-40, Method: Compositional matrix adjust. Identities = 94/287 (32%), Positives = 158/287 (55%), Gaps = 8/287 (2%) Query: 2 IGLGAMGYGVASSLLRAGFNVHACDVRKEVLDKFAAAGGVACANPAELAGKVDVVITLVV 61 IGLG MG +A +L++AGF+V D+ K +DK GG A+ K DVVIT++ Sbjct: 8 IGLGNMGLPMAQNLVKAGFSVSGHDLVKASVDKLVEVGGKTEADSMAAVAKADVVITMLP 67 Query: 62 NAAQTETVLFGDNGAVSAMKPGSVVISSATVAPDFAIELGKRLAEKGLLLLDAPVSGGAA 121 + E++ G+ G +++ KPG+++I +T+AP+ A ++ +G +LDAPVSGG Sbjct: 68 ASRHVESLYLGEAGVLASAKPGTLLIDCSTIAPEAARKVAAAAKARGFEMLDAPVSGGTN 127 Query: 122 RAASGEMTMMTSGPAEVYAKIEDVLAGMAGKVYRLGDTHGIGSKVKIINQLLAGVHIAAS 181 A +G +T M G E + + L M +Y G++ G G VK+ N +L G+ + + Sbjct: 128 GATAGTLTFMVGGSKEAFEAAQPYLQKMGKAIYHAGES-GSGQTVKVCNNMLLGILMIGT 186 Query: 182 AEAMALGLREGVNPDALYEVITHSAGNSWMFE--NRVPHIL-----NGDYTPLSAVDIFV 234 +EA+ LG+ G++P L E+++ S+G +W E N P ++ + Y VD+ + Sbjct: 187 SEAIRLGMANGMDPKVLSEIMSKSSGRNWTLEVYNPCPGVMETAPASKGYAGGFGVDLML 246 Query: 235 KDLGLVLDTARRSKFPLPLSAAAHQMFMMASTAGHGGEDDSAVIKIF 281 KDLGL ++ A + +PL A A ++ + S G G D S++ + Sbjct: 247 KDLGLAVENALATNCAIPLGATARNLYDIHSMNGAGKLDFSSIFNML 293 >gi|300310292|ref|YP_003774384.1| 3-hydroxyisobutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 288 Score = 108 bits (269), Expect = 8e-25, Method: Compositional matrix adjust. Identities = 82/262 (31%), Positives = 131/262 (50%), Gaps = 2/262 (0%) Query: 1 VIGLGAMGYGVASSLLRAGFNVHACDVRKEVLDKFAAAGGVACANPAELAGKVDVVITLV 60 VIGLG MG G+A SL R G+ V D ++ A G + A LA V++V+ + Sbjct: 6 VIGLGNMGRGMAFSLQRGGYEVLGYDAQESTRTALAQQGLHCVDSIAALAPAVEMVVLSL 65 Query: 61 VNAAQTETVLFGDNGAVSAMKPGSVVISSATVAPDFAIELGKRLAEKGLLLLDAPVSGGA 120 + E V+ G+ G + PG V++ + T P+ L RL G+ L+DAPVSGG Sbjct: 66 PTSDIVEQVVEGEGGLLQHGAPGLVIVDTTTADPNSTRRLAARLQAAGMELVDAPVSGGP 125 Query: 121 ARAASGEMTMMTSGPAEVYAKIEDVLAGMAGKVYRLGDTHGIGSKVKIINQLLAGVHIAA 180 AA+ M M+ G AE A+IE VLA M+ K +G G G K++N L+ G H+ Sbjct: 126 KGAATATMGMVIGGSAETVARIEPVLAVMSAKRVHVGPV-GAGHVAKLLNNLVTGTHLLI 184 Query: 181 SAEAMALGLREGVNPDALYEVITHSAGNSWMFENRVP-HILNGDYTPLSAVDIFVKDLGL 239 + EA + G++ +++ + + NS + E P I N + + + KDL L Sbjct: 185 AGEAARIAAAAGLDARQIFQGLAAGSANSKVIEVFYPTWIFNEAFDSGFTMKLMRKDLRL 244 Query: 240 VLDTARRSKFPLPLSAAAHQMF 261 ++ ++ PL+ A +++ Sbjct: 245 AMNLIEETRSTTPLAREAGRLW 266 >gi|300311332|ref|YP_003775424.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 292 Score = 74.3 bits (181), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 75/279 (26%), Positives = 146/279 (52%), Gaps = 2/279 (0%) Query: 2 IGLGAMGYGVASSLLRAGFNVHACDVRKEVLDKFAAAGGVACANPAELAGKVDVVITLVV 61 +GLG MG+ +A++L+RAG+ V A + +++ AA G A A PAE A DV+I+++ Sbjct: 6 LGLGTMGFPMAANLVRAGYAVRAWNRSPAPVERLAALGAQAAATPAEAAADADVLISMLA 65 Query: 62 NAAQTETVLFGDNGAVSAMKPGSVVISSATVAPDFAIELGKRLAEKGLLLLDAPVSGGAA 121 + TET L A++A++PG++ ++ AT++ A+ L +G+ + APV G Sbjct: 66 DDDATETSLLDAG-ALAALRPGAIHVNMATISVALALRLAGLHQARGVAYVAAPVLGRVN 124 Query: 122 RAASGEMTMMTSGPAEVYAKIEDVLAGMAGKVYRLGDTHGIGSKVKIINQLLAGVHIAAS 181 A +G++ ++ +G + A ++ +L + K +RLG + K+ + I A Sbjct: 125 VAEAGQLNILAAGEEQALAAVQPLLDVLGQKTWRLGRQPEQANAAKLAMNFMIASAIGAM 184 Query: 182 AEAMALGLREGVNPDALYEVITHSAGNSWMFENRVPHILNGDYTPLS-AVDIFVKDLGLV 240 +EA+AL G++ E+ T +A + +++ I + + P + + +KD+ L Sbjct: 185 SEAVALVQGYGLDKAGFIELATSTAFAAPVYKGYGQAIADDRFEPAGFKLALGLKDVRLA 244 Query: 241 LDTARRSKFPLPLSAAAHQMFMMASTAGHGGEDDSAVIK 279 L+ ++ PL L++A + + G G D +A+ + Sbjct: 245 LEAGEQAHVPLSLASALRDLHIDGLAHGEGHLDWAALSR 283 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.134 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 913,507 Number of extensions: 36763 Number of successful extensions: 178 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 173 Number of HSP's successfully gapped: 4 Length of query: 292 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 208 Effective length of database: 1,222,130 Effective search space: 254203040 Effective search space used: 254203040 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)