BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0243.p (437 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 878 0.0 gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 520 e-149 gi|300311164|ref|YP_003775256.1| major facilitator superfamily p... 385 e-108 gi|300313342|ref|YP_003777434.1| major facilitator superfamily p... 374 e-105 gi|300309876|ref|YP_003773968.1| major facilitator superfamily p... 352 4e-98 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 878 bits (2269), Expect = 0.0, Method: Compositional matrix adjust. Identities = 436/437 (99%), Positives = 436/437 (99%) Query: 1 MTTASTTGVCLDVNDKSKDGLYKKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSQDLGF 60 MTTASTTGV LDVNDKSKDGLYKKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSQDLGF Sbjct: 1 MTTASTTGVGLDVNDKSKDGLYKKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSQDLGF 60 Query: 61 SETVFGLGAGLFFIGYFLFEVPSNILMHKVGARIWIARIMITWGILSAAFMFVQNATQFY 120 SETVFGLGAGLFFIGYFLFEVPSNILMHKVGARIWIARIMITWGILSAAFMFVQNATQFY Sbjct: 61 SETVFGLGAGLFFIGYFLFEVPSNILMHKVGARIWIARIMITWGILSAAFMFVQNATQFY 120 Query: 121 ILRFLLGLAEAGFYPGIILYLTYWFPSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDAFH 180 ILRFLLGLAEAGFYPGIILYLTYWFPSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDAFH Sbjct: 121 ILRFLLGLAEAGFYPGIILYLTYWFPSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDAFH 180 Query: 181 QNGGLEGWQWMFLIEAIPAVLIGVATVLYLDNDVKSAKWLNDEEKASLQADIDGDAKGKE 240 QNGGLEGWQWMFLIEAIPAVLIGVATVLYLDNDVKSAKWLNDEEKASLQADIDGDAKGKE Sbjct: 181 QNGGLEGWQWMFLIEAIPAVLIGVATVLYLDNDVKSAKWLNDEEKASLQADIDGDAKGKE 240 Query: 241 SKHGFGAIVKDARVWLMCLIYFSFVMGQYGLTLWMPTLVKATGVKGNLEIGLLSAIPFGC 300 SKHGFGAIVKDARVWLMCLIYFSFVMGQYGLTLWMPTLVKATGVKGNLEIGLLSAIPFGC Sbjct: 241 SKHGFGAIVKDARVWLMCLIYFSFVMGQYGLTLWMPTLVKATGVKGNLEIGLLSAIPFGC 300 Query: 301 AIIAMNLIGRSADRMRERRWHLVIPALMGGVGFVGAALFADNTAVSIASLSLAAAGVLTC 360 AIIAMNLIGRSADRMRERRWHLVIPALMGGVGFVGAALFADNTAVSIASLSLAAAGVLTC Sbjct: 301 AIIAMNLIGRSADRMRERRWHLVIPALMGGVGFVGAALFADNTAVSIASLSLAAAGVLTC 360 Query: 361 APLFWSLPTAFLSGAAAAVGIAAINSVGNLAGFVSPYLIGYLKDLTHNNATGMYMLAVML 420 APLFWSLPTAFLSGAAAAVGIAAINSVGNLAGFVSPYLIGYLKDLTHNNATGMYMLAVML Sbjct: 361 APLFWSLPTAFLSGAAAAVGIAAINSVGNLAGFVSPYLIGYLKDLTHNNATGMYMLAVML 420 Query: 421 VVGSIATLATKPGMVNK 437 VVGSIATLATKPGMVNK Sbjct: 421 VVGSIATLATKPGMVNK 437 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 520 bits (1339), Expect = e-149, Method: Compositional matrix adjust. Identities = 247/420 (58%), Positives = 323/420 (76%), Gaps = 1/420 (0%) Query: 18 KDGLYKKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSQDLGFSETVFGLGAGLFFIGYF 77 +D Y KV WR++P L LCYV +YLDRVNVGFAKLQM QDL FS+TV+GLGAG+FF+GYF Sbjct: 17 EDATYSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGYF 76 Query: 78 LFEVPSNILMHKVGARIWIARIMITWGILSAAFMFVQNATQFYILRFLLGLAEAGFYPGI 137 +FEVPSN+++HKVGAR+WIARIMITWGI+S A +V FYI+RFLLG+AEAGF+PG+ Sbjct: 77 VFEVPSNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYIMRFLLGVAEAGFFPGV 136 Query: 138 ILYLTYWFPSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDAFHQNGGLEGWQWMFLIEAI 197 ILYLTYW+P+ RR K+ A+FM+GI ++G++G PLSGWIM A G GWQWMF++EAI Sbjct: 137 ILYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPGVWGHTGWQWMFILEAI 196 Query: 198 PAVLIGVATVLYLDNDVKSAKWLNDEEKASLQADIDGDAKGKESKHGFGAIVKDARVWLM 257 P++++GV +LY+ + ++ A WL++EEK L+A+I + KE GA+ + RVWL Sbjct: 197 PSLILGVMVILYMKDRIRDADWLSEEEKRLLEANISHEESQKE-HLSLGAMFANGRVWLG 255 Query: 258 CLIYFSFVMGQYGLTLWMPTLVKATGVKGNLEIGLLSAIPFGCAIIAMNLIGRSADRMRE 317 LIYF FVMG YG++ W+PT++KATGV L +GLL+AIP+ A AM LIGRSAD RE Sbjct: 256 ALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSADARRE 315 Query: 318 RRWHLVIPALMGGVGFVGAALFADNTAVSIASLSLAAAGVLTCAPLFWSLPTAFLSGAAA 377 RRWH+ IPA +G +G + + + NT +++ASL+LA+ G+LT PLFWSLPTAFLSG AA Sbjct: 316 RRWHVAIPAFIGCIGLLLSTQYGHNTTLAMASLTLASVGILTTLPLFWSLPTAFLSGTAA 375 Query: 378 AVGIAAINSVGNLAGFVSPYLIGYLKDLTHNNATGMYMLAVMLVVGSIATLATKPGMVNK 437 A GIA INS+GNLAGFVSP+L+G+LKD T + GMY+LA LV+G + TL +VN+ Sbjct: 376 AAGIALINSLGNLAGFVSPFLVGWLKDATQSTNAGMYVLAASLVIGGLLTLTLPAKLVNR 435 >gi|300311164|ref|YP_003775256.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 385 bits (989), Expect = e-108, Method: Compositional matrix adjust. Identities = 197/403 (48%), Positives = 274/403 (67%) Query: 22 YKKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSQDLGFSETVFGLGAGLFFIGYFLFEV 81 Y+K+ WR++PFL+ +V+A++DRVNVGFAKLQM QDL FSE V+GLGAG+FFIGYF+FEV Sbjct: 25 YRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAVYGLGAGIFFIGYFIFEV 84 Query: 82 PSNILMHKVGARIWIARIMITWGILSAAFMFVQNATQFYILRFLLGLAEAGFYPGIILYL 141 PSN+L+ K+GAR +ARI I WG+ S A +V T FYILRFLLG+ EAGF+PG++LYL Sbjct: 85 PSNLLLEKIGARKTLARITILWGLASMAMAYVTTPTSFYILRFLLGIFEAGFFPGVVLYL 144 Query: 142 TYWFPSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDAFHQNGGLEGWQWMFLIEAIPAVL 201 TYWFP+ RRA+V +FM+ +AG +G P++G IM G L WQW+F++E IP++L Sbjct: 145 TYWFPAQRRARVNGLFMTSFAIAGAVGGPIAGLIMSGMEGVGHLANWQWLFILEGIPSIL 204 Query: 202 IGVATVLYLDNDVKSAKWLNDEEKASLQADIDGDAKGKESKHGFGAIVKDARVWLMCLIY 261 G A + +L +AKWL++ EK ++ + + F + RVW+ +Y Sbjct: 205 AGFAVLAWLPERPANAKWLSEAEKNAVTQVLAAENSAPGKHFSFKDACTNYRVWICAAVY 264 Query: 262 FSFVMGQYGLTLWMPTLVKATGVKGNLEIGLLSAIPFGCAIIAMNLIGRSADRMRERRWH 321 F V G + W P+++K GVKGNL+IGL+SAIPF +AM G +D+ ERR H Sbjct: 265 FCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISAIPFIAGTLAMIWNGFHSDKTGERRLH 324 Query: 322 LVIPALMGGVGFVGAALFADNTAVSIASLSLAAAGVLTCAPLFWSLPTAFLSGAAAAVGI 381 I L+ +G V + N +++ SL+LAA G+L P+FWS+P AFL+G AAA GI Sbjct: 325 SAIATLIACIGLVATGMTLGNATLALCSLTLAAIGILAAFPVFWSIPGAFLAGTAAAGGI 384 Query: 382 AAINSVGNLAGFVSPYLIGYLKDLTHNNATGMYMLAVMLVVGS 424 A INS+GNLAGFV+PY+IG LK T + ++G+Y +A + +V S Sbjct: 385 ALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVAALELVAS 427 >gi|300313342|ref|YP_003777434.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 374 bits (960), Expect = e-105, Method: Compositional matrix adjust. Identities = 198/407 (48%), Positives = 270/407 (66%), Gaps = 10/407 (2%) Query: 19 DGLYKKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSQDLGFSETVFGLGAGLFFIGYFL 78 DGLYKKV W+I+P+L+L YVIAYLDRVNVGFAKL M DLG SE +GLGAG+FF+GYF+ Sbjct: 13 DGLYKKVTWKILPWLLLGYVIAYLDRVNVGFAKLSMLSDLGMSEAAYGLGAGIFFLGYFI 72 Query: 79 FEVPSNILMHKVGARIWIARIMITWGILSAAFMF---------VQNATQFYILRFLLGLA 129 FEVPSN+++H+VGA+IWIARIMITWG++S+ F V N T FY LRFLLG + Sbjct: 73 FEVPSNLILHRVGAKIWIARIMITWGMISSLMAFTAPIAQWLGVSNETVFYTLRFLLGAS 132 Query: 130 EAGFYPGIILYLTYWFPSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDAFHQNGGLEGWQ 189 EAGF+PG+ILY+TYW+P+ ++V+AV + G P + ILG P+SG +M L GWQ Sbjct: 133 EAGFFPGVILYMTYWYPAAMHSRVMAVVLLGNPFSYILGGPISGALMQFMGSGDSLAGWQ 192 Query: 190 WMFLIEAIPAVLIGVATVLYLDNDVKSAKWLNDEEKASLQADIDGDAKGKESKHGFGAIV 249 WMF+IEA+PA L+G+ + L ND+ SAKWL EEKA L + + K K + ++ Sbjct: 193 WMFVIEALPACLLGIVILFVLSNDIASAKWLAPEEKAELARRLAAENKEKVNVP-LKSLY 251 Query: 250 KDARVWLMCLIYFSFVMGQYGLTLWMPTLVKATGVKGNLEIGLLSAIPFGCAIIAMNLIG 309 +W + L+Y ++G YG+ W PT+++ GVK L+IG++SAIP+ AM I Sbjct: 252 ALPILWFLALVYLLMMIGSYGINFWAPTIIRDAGVKSVLDIGMISAIPYILGATAMVFIT 311 Query: 310 RSADRMRERRWHLVIPALMGGVGFVGAALFADNTAVSIASLSLAAAGVLTCAPLFWSLPT 369 R A++ + R + V +++ G+ V LF N +++A +++A G LT LFW P Sbjct: 312 RHAEKAGKHRSYSVAVSMVAGLALVLCTLFPHNIYITLAGIAIAVGGSLTSIALFWGFPG 371 Query: 370 AFLSGAAAAVGIAAINSVGNLAGFVSPYLIGYLKDLTHNNATGMYML 416 A+ +GA AA GIA INSVGNL GFV PYL+G L + G+ ML Sbjct: 372 AYFTGATAAAGIAIINSVGNLGGFVGPYLLGALTTAFGSPRYGLMML 418 >gi|300309876|ref|YP_003773968.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 449 Score = 352 bits (902), Expect = 4e-98, Method: Compositional matrix adjust. Identities = 183/415 (44%), Positives = 255/415 (61%), Gaps = 9/415 (2%) Query: 3 TASTTGVCLDVN----DKSKDGLYKKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSQDL 58 T S+ G L +++D +Y+KV+ RI+PFL +CYV+++LDR+N+GFA+LQM QDL Sbjct: 2 TPSSGGATLSTTPSLASQAEDAIYRKVWLRIIPFLFICYVVSFLDRINIGFAQLQMKQDL 61 Query: 59 GFSETVFGLGAGLFFIGYFLFEVPSNILMHKVGARIWIARIMITWGILSAAFMFVQNATQ 118 GFS+ ++GLGA +F++GY LFEVPSN+ + + GAR +RIM+ WG+ S M V +A Sbjct: 62 GFSDAMYGLGAAIFYVGYVLFEVPSNMALARFGARRTFSRIMVLWGLASVGMMAVTDAAH 121 Query: 119 FYILRFLLGLAEAGFYPGIILYLTYWFPSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDA 178 FY LRFLLG+ EAGF+PGI+LYL+YWFP+ RRA V+A+F +G+ VAG+LG +SGWIM Sbjct: 122 FYTLRFLLGVFEAGFFPGIVLYLSYWFPARRRAAVMAIFFAGVAVAGVLGGLVSGWIMRD 181 Query: 179 FHQNGGLEGWQWMFLIEAIPAVLIGVATVLYLDNDVKSAKWLNDEEKASLQADIDGDAKG 238 GL GWQWMF IE PAV++G+ + L + AKWL+ EE++ LQA +A Sbjct: 182 MAGVMGLYGWQWMFAIEGAPAVVLGLLAIFLLADGPAQAKWLSPEERSFLQARHQQEALR 241 Query: 239 KE--SKHGFGAI---VKDARVWLMCLIYFSFVMGQYGLTLWMPTLVKATGVKGNLEIGLL 293 S H A+ + +L IYF+ G L WMP +++ G+K +E+ L Sbjct: 242 THHVSGHSLAALRQALSHRTTYLFAFIYFALTCGSLTLNFWMPLMIRDFGIKDVVEVSLY 301 Query: 294 SAIPFGCAIIAMNLIGRSADRMRERRWHLVIPALMGGVGFVGAALFADNTAVSIASLSLA 353 S IP + + LI R +DR ER H + G + L + AVS+ LS+A Sbjct: 302 SVIPNAIGAVGLILIARRSDRRNERHGHFAVCTTGGALALAALTLHPSSFAVSLGLLSVA 361 Query: 354 AAGVLTCAPLFWSLPTAFLSGAAAAVGIAAINSVGNLAGFVSPYLIGYLKDLTHN 408 A + P+FWSLP L A GIA I+SVG +G VSP+LIG +K T N Sbjct: 362 AVLIFAALPIFWSLPPGLLPAQTIAPGIAFISSVGITSGIVSPWLIGLVKTRTGN 416 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.141 0.441 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,268,608 Number of extensions: 51906 Number of successful extensions: 306 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 274 Number of HSP's successfully gapped: 21 Length of query: 437 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 349 Effective length of database: 1,203,190 Effective search space: 419913310 Effective search space used: 419913310 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)