BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0232.p (349 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313504|ref|YP_003777596.1| galactose-1-phosphate uridylylt... 435 e-123 gi|300313507|ref|YP_003777599.1| polysaccharide biosynthesis pro... 142 5e-35 gi|300313483|ref|YP_003777575.1| NAD_dependent epimerase/dehydra... 67 2e-12 gi|300311327|ref|YP_003775419.1| UDP-glucose 4-epimerase [Herbas... 57 2e-09 >gi|300313504|ref|YP_003777596.1| galactose-1-phosphate uridylyltransferase [Herbaspirillum seropedicae SmR1] Length = 336 Score = 435 bits (1119), Expect = e-123, Method: Compositional matrix adjust. Identities = 204/335 (60%), Positives = 260/335 (77%) Query: 7 FQNATILITGGTGSFGNALLDLLKNSQVGQIRIFSRDELKQELMRIRYNDPRLRFYIGDV 66 F ++ ++ITGGTGSFG +L+ N+ V +IR+FSRDE KQE +RI N+ +LRFYIGDV Sbjct: 2 FDDSVLMITGGTGSFGQTVLNRFLNTGVREIRVFSRDEKKQEDLRIALNNEKLRFYIGDV 61 Query: 67 RDRASLDGAMSGVDYVFHAAALKQVPSCEFFPMQAVQTNVIGSMNVVESAIKNNVKKAVF 126 RD SL AM GVDYVFHAAALKQVPSCEF+PM+AV+TNVIG+ NV+ + + V++ V Sbjct: 62 RDPDSLRAAMKGVDYVFHAAALKQVPSCEFYPMEAVKTNVIGTENVLRVSSECGVRRVVV 121 Query: 127 LSTDKAVYPVNAMGMTKALMEKITGAAARAAVEGNTVLSSVRYGNVMYSRGSVIPLFIEQ 186 LSTDKAVYP+NAMG++KA+ EK+ AAAR +G TV + RYGNVM SRGSVIPLF+ Q Sbjct: 122 LSTDKAVYPINAMGISKAMAEKVMVAAARTQRQGETVFCATRYGNVMASRGSVIPLFVSQ 181 Query: 187 IKAGKPLTLTVPEMTRFLLPLPHAIELVTFAFQHANQGDLFVKKAPACTVQMLAQALLEL 246 IKA KP+T+T P MTRFL+ L +++LV +AFQH QGD+FV+KAPA TV LA AL EL Sbjct: 182 IKAEKPITITDPNMTRFLMSLEDSVDLVLYAFQHGQQGDIFVQKAPASTVADLASALKEL 241 Query: 247 FGSSVPLKVIGMRHGEKIYETLATREELAKAEDMGDYYRVPMDDRDLNYGKYFTEGDTKE 306 F S +K IG RHGEK+YE+L +REE+A A+DMGDYYR+P D+RDLNY KYF+EGD Sbjct: 242 FSSDTVVKTIGTRHGEKLYESLISREEMAHAQDMGDYYRIPADNRDLNYAKYFSEGDQDI 301 Query: 307 PTLDDYHSHNTQQLDLQEVKQLLLTLPQVRAELQA 341 DDY SHNT++L+++++KQLLL L ++ EL A Sbjct: 302 SLFDDYTSHNTRRLNVEQIKQLLLGLDFIKEELNA 336 >gi|300313507|ref|YP_003777599.1| polysaccharide biosynthesis protein [Herbaspirillum seropedicae SmR1] Length = 648 Score = 142 bits (357), Expect = 5e-35, Method: Compositional matrix adjust. Identities = 96/282 (34%), Positives = 151/282 (53%), Gaps = 21/282 (7%) Query: 6 DFQNATILITGGTGSFGNALLDLLKNSQVGQIRIFSRDE-----LKQELM-RIRYNDPRL 59 + ++ +++TG GS G+ L + ++ + + E + Q+L ++ D L Sbjct: 287 NVRSKVVMVTGAGGSIGSELCRQILAVGPSKLLLIEQSEFALYAIHQQLEEKLADRDIVL 346 Query: 60 RFYIGDVRDRASLDGAMSGV--DYVFHAAALKQVPSCEFFPMQAVQTNVIGSMNVVESAI 117 + V+D + MS D V+HAAA K VP E P + ++ NV+G++ ++A Sbjct: 347 VPLLASVQDSERIREIMSTWHPDTVYHAAAYKHVPLVEHNPAEGIKNNVLGTLRTAQAAT 406 Query: 118 KNNVKKAVFLSTDKAVYPVNAMGMTKALMEKITGAAARAAVEGNTVLSSVRYGNVMYSRG 177 +N V V +STDKAV P N MG +K L E + A AA T S VR+GNV+ S G Sbjct: 407 ENGVADFVLISTDKAVRPTNIMGASKRLAEMVLQAI--AATSPGTKFSMVRFGNVLGSSG 464 Query: 178 SVIPLFIEQIKAGKPLTLTVPEMTRFLLPLPHAIELVTFAFQHANQGDLFV-KKAPACTV 236 SV+P F +QI+ G P+TLT ++TR+ + +P A +LV A A GD+FV A + Sbjct: 465 SVVPKFRQQIRDGGPITLTHADITRYFMTIPEASQLVIQAGAMAKGGDVFVLDMGQAVRI 524 Query: 237 QMLAQALLELFGSSV----------PLKVIGMRHGEKIYETL 268 LA+ ++EL G +V +++ G+R GEK+YE L Sbjct: 525 MDLARRMIELSGLTVKDELNPEGDIAIEITGLRPGEKLYEEL 566 >gi|300313483|ref|YP_003777575.1| NAD_dependent epimerase/dehydratase [Herbaspirillum seropedicae SmR1] Length = 333 Score = 67.4 bits (163), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 46/137 (33%), Positives = 68/137 (49%), Gaps = 15/137 (10%) Query: 6 DFQNATILITGGTGSFGNALLDLLKNSQVGQIRIF------SRDELKQELMRIRYNDPRL 59 D I++ GG G G+ +D L V +I I+ SR+ L+ L DPR+ Sbjct: 2 DLTGKKIVVIGGAGLIGSHTVDHLVKEDVKEILIYDNFLRGSRENLQNAL-----KDPRV 56 Query: 60 RFYI--GDVRDRASLDGAMSGVDYVFHAAALKQVPSCEFFPMQAVQTNVIGSMNVVESAI 117 + Y GD+ L A+ G D VFH AAL + C +P A NV G+ NV+E+ + Sbjct: 57 KIYDVGGDIMQTDILQSALEGADGVFHLAAL-WLLQCHEYPRSAFDVNVRGTFNVMEACV 115 Query: 118 KNNVKKAVFLSTDKAVY 134 VK+ V+ S+ +VY Sbjct: 116 AKGVKRLVY-SSSASVY 131 >gi|300311327|ref|YP_003775419.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 343 Score = 57.4 bits (137), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 54/171 (31%), Positives = 74/171 (43%), Gaps = 40/171 (23%) Query: 10 ATILITGGTGSFGN-------------ALLDLLKNSQVGQIRIFSRDELKQELMRIRYND 56 ATIL+TGG G G+ +LD L NS+ I D + Q + Sbjct: 4 ATILVTGGAGYIGSHTCVELIHAGYAVVVLDNLCNSRASVI-----DRIAQ------ISG 52 Query: 57 PRLRFYIGDVRDRASLDGAMSG--VDYVFHAAALKQVPSCEFFPMQAVQTNVIGSMNVVE 114 R F GD+RDRA LDG ++ V H A LK V P++ NV GS + E Sbjct: 53 QRPHFVQGDIRDRAVLDGIFKSHRIEAVIHFAGLKAVGESVAQPLRYYDNNVHGSNVLFE 112 Query: 115 SAIKNNVKKAVFLST-----DKAVYPV---------NAMGMTKALMEKITG 151 + + VK VF S+ D A P+ N G +K ++E+I G Sbjct: 113 AMAAHGVKNIVFSSSATVYGDPASVPITEEFPLSATNPYGRSKLMVEQILG 163 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,012,324 Number of extensions: 40036 Number of successful extensions: 134 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 132 Number of HSP's successfully gapped: 4 Length of query: 349 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 263 Effective length of database: 1,212,660 Effective search space: 318929580 Effective search space used: 318929580 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)