BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0221.p (315 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312049|ref|YP_003776141.1| UDP-glucose 4-epimerase [Herbas... 69 6e-13 gi|300313692|ref|YP_003777784.1| UDP-glucose 4-epimerase [Herbas... 64 2e-11 gi|300312786|ref|YP_003776878.1| nucleoside-diphosphate-sugar ep... 59 6e-10 gi|300309817|ref|YP_003773909.1| UDP-glucose 4-epimerase [Herbas... 57 2e-09 >gi|300312049|ref|YP_003776141.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 347 Score = 68.6 bits (166), Expect = 6e-13, Method: Compositional matrix adjust. Identities = 63/234 (26%), Positives = 101/234 (43%), Gaps = 28/234 (11%) Query: 5 LITGGLGYLGGRIASQLVKAGHRVR-----ATSRKAQ--------PAPAWLPEMQVLAPD 51 LITG G++G + ++L+ G +V +T R+A A AW + + D Sbjct: 19 LITGVAGFIGSNLLARLLTLGQQVTGLDNFSTGRQANLEEVRAEVGAAAW-ANFRFIEGD 77 Query: 52 WESTDELIAACTGHDAVVHLAAMNEIY-SGRDPVGALRTNGLASLMLQNAAQSAGVRRFI 110 + AC G D V+H AA+ + S DPV N L + AA+ AGV RF+ Sbjct: 78 ICDAATCLRACDGADYVLHQAALGSVSRSIEDPVRTNLNNVNGFLNMLVAARDAGVARFV 137 Query: 111 FFSTAHVYGAPLAGDIDEATLARPRHPYAITHRVAEDFVLAAHDSGQIEGVVVRLSNGFG 170 + +++ YG E + +P PYA+T V E + +E + +R N FG Sbjct: 138 YAASSSTYGDHPGLPKVEEVIGKPLSPYAVTKYVNELYAEVFARCYGMESIGLRYFNVFG 197 Query: 171 ------APMTADVDRWTLLVNDLCRQALTTGALKLQSAGTQLRDFITLDDVGRA 218 A + +W + + G L++ G RDF +D+V +A Sbjct: 198 RRQDPHGAYAAVIPQWVTAM-------IRNGELRINGDGQTSRDFCYIDNVVQA 244 >gi|300313692|ref|YP_003777784.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 336 Score = 63.9 bits (154), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 80/300 (26%), Positives = 127/300 (42%), Gaps = 40/300 (13%) Query: 3 SILITGGLGYLGGRIASQLVKAGHRVRATSRKAQPAPAWLPEMQ--------VLAPDWES 54 +IL+TGG GY+G +L+KAGH V +P + +Q ++ D + Sbjct: 2 NILVTGGAGYIGSHTCVELLKAGHEVIVFDNFCNSSPESVKRVQQITGKTLSLVKGDIRN 61 Query: 55 TDELIAACTGH--DAVVHLAAMNEI-YSGRDPVGALRTNGLASLMLQNAAQSAGVRRFIF 111 ++L + +AV+H A + + S P+ N + ++ L A Q+ GV+ +F Sbjct: 62 REQLESVLREFKCEAVIHFAGLKAVGESVEQPLMYYDNNVVGTVRLLEAMQATGVKTIVF 121 Query: 112 FSTAHVYGAPLAGDIDEATLARPRHPYAITHRVAEDFV---LAAHDSGQIEGVVVRLSNG 168 S+A VYG P +DEA +PY + V ED + AH +I ++R N Sbjct: 122 SSSATVYGDPQYLPLDEAHPLSASNPYGRSKIVIEDMLRDYYRAHPDWKI--ALLRYFNP 179 Query: 169 FGAPMTADV-DRWTLLVNDLC----------RQALTT-GALKLQSAGTQLRDFITLDDVG 216 GA + + + + N+L R+ L G GT +RDFI + D+ Sbjct: 180 VGAHESGLIGEDPGGIPNNLMPFVAQVAIGRRERLNIWGGDYPTPDGTGVRDFIHVVDLA 239 Query: 217 RA-VQHLVELDTS-----SLGNGLFNLGDRTMSILQMTEMVAARASKLLGRDIPIIRPAD 270 R V L LD +LG G+ S+L + + K L I RP D Sbjct: 240 RGHVAALQALDAPQCFAVNLGTGV------GYSVLDVLKAYEKACGKTLPYAIAERRPGD 293 >gi|300312786|ref|YP_003776878.1| nucleoside-diphosphate-sugar epimerase [Herbaspirillum seropedicae SmR1] Length = 310 Score = 58.5 bits (140), Expect = 6e-10, Method: Compositional matrix adjust. Identities = 59/229 (25%), Positives = 98/229 (42%), Gaps = 23/229 (10%) Query: 1 MASILITGGLGYLGGRIASQLVKAGHRVRATSRKAQPAPAWL---PEMQVLAPDWESTDE 57 M+ I + G G +G IAS + G R R + P ++++ D +S Sbjct: 1 MSKIALFGAAGAIGRSIASAISAQGQHYRVVGRSEETLRQTFGADPRAEIVSWDPDSPAS 60 Query: 58 LIAACTGHDAVVHLAAMNEIYSGRDPVGALRTNGLASLMLQNAAQSAGVRRFIFFSTAHV 117 + AA G D +++L ++ Y+ + L LA A SAGVR+ + T + Sbjct: 61 VEAAAHGIDTLIYLVGVH--YTRFELHPELMRKTLA------GAISAGVRQILLIGTVYP 112 Query: 118 YGAPLAGDIDEATLARPRHPYAITHRV---AEDFVLAAHDSGQIEGVVVRLSNGFGAPMT 174 YG P + E PR P+ R+ ED ++ AH G+I V+RL + +G + Sbjct: 113 YGLPQTTPVRED---HPRAPHTFKGRMRKAQEDLLMQAHTEGRIRAAVLRLPDFYGPGVE 169 Query: 175 ADVDRWTLLVNDLCRQALTTGALKLQSAGTQLRDFITLDDVGRAVQHLV 223 A L++ + A G + + +F+ + DVG V LV Sbjct: 170 AS------LLHGAIKAAAQGGTADMIGPLDRPHEFVFVPDVGPVVAKLV 212 >gi|300309817|ref|YP_003773909.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 320 Score = 57.0 bits (136), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 59/193 (30%), Positives = 88/193 (45%), Gaps = 22/193 (11%) Query: 4 ILITGGLGYLGGRIASQLVKAG-HRVRATSRKAQPAPAWLPEMQ-VLAPDWESTDELIAA 61 IL+TG G++G + S+L G H +R R + ++ + + + AA Sbjct: 3 ILVTGASGFVGRALLSRLDSEGQHLLRGAFRPSSETVMDRERIERTVISGLDPETDWSAA 62 Query: 62 CTGHDAVVHLAA----MNEIYSGRDPVGALRTNGL-ASLMLQNAAQSAGVRRFIFFSTAH 116 +AVVH AA MNE + DP+ R L SL L AQ AGV+RFIF S+ Sbjct: 63 LHNIEAVVHCAARVHVMNE--TAGDPLAEFRRINLEGSLALARQAQQAGVQRFIFLSSIK 120 Query: 117 V------YGAPLAGDIDEATLARPRHPYAITHRVAEDFVLAAHDSGQIEGVVVRLSNGFG 170 V G P D A P PY I+ AE +L +G+++ V++R +G Sbjct: 121 VNGEQTSLGRPFTADDKPA----PTDPYGISKMEAEQALLELGRTGKMQVVIIRPVLVYG 176 Query: 171 APMTAD---VDRW 180 + A+ + RW Sbjct: 177 PGVKANFLSMMRW 189 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 940,496 Number of extensions: 39896 Number of successful extensions: 147 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 146 Number of HSP's successfully gapped: 5 Length of query: 315 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 230 Effective length of database: 1,217,395 Effective search space: 280000850 Effective search space used: 280000850 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)