BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0219.p (298 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313691|ref|YP_003777783.1| CDP-glucose-4,6-dehydratase [He... 244 8e-66 gi|300313692|ref|YP_003777784.1| UDP-glucose 4-epimerase [Herbas... 77 2e-15 gi|300311327|ref|YP_003775419.1| UDP-glucose 4-epimerase [Herbas... 55 6e-09 >gi|300313691|ref|YP_003777783.1| CDP-glucose-4,6-dehydratase [Herbaspirillum seropedicae SmR1] Length = 357 Score = 244 bits (622), Expect = 8e-66, Method: Compositional matrix adjust. Identities = 137/294 (46%), Positives = 183/294 (62%), Gaps = 9/294 (3%) Query: 6 GDVRDLASLNKAVQSFKPEFVFHLAAQALVRESYSDPKSTFDTNVGGSVNLLEAVRACDS 65 GDVRD L + + + +P+ VFHLA Q + SY P TF TNV G+ +LLEAVR+ S Sbjct: 62 GDVRDPKHLAETMAAVQPDVVFHLAGQPQIEHSYLTPLDTFATNVMGTAHLLEAVRSTPS 121 Query: 66 VRSLVFVTSDKCYENVEWIWAYRENDALGGRDPYSASKAAAEIVFSSYLRSWFTTHPH-- 123 VR++V V+S+KCYEN EW+W YRE+D +GG DP+S+SK AAE+V ++Y RS+F+ P Sbjct: 122 VRAVVVVSSNKCYENREWLWGYREDDPMGGHDPFSSSKGAAELVTAAYRRSFFSADPDSP 181 Query: 124 LGAASVRAGNVIGGGDWAADRIVPDCIRALVDKQAIVLRNPAATRPWQHVLEPISGYLKL 183 +G ASVR GNVIGGGD+AADR++PD IRAL Q + +R P A RPWQ VLEP+SGYL++ Sbjct: 182 VGVASVRTGNVIGGGDFAADRLIPDFIRALETDQPVTIRQPHAVRPWQFVLEPLSGYLQI 241 Query: 184 AAYLYDAPHQYEGAWNFGPPVGDARTVLDVAKHLATQFVDG-----QVVVEASANKLHEA 238 LY+ Y AWNFGP D +TV + +V +E + L EA Sbjct: 242 GKMLYEHGASYAEAWNFGPTDTDLQTVSQLCATFNHSLQKNDVEPVEVRLEQAGATLSEA 301 Query: 239 NLLQLNSDKARQMLQWSTRWDFEQTLQATGNWYQEVLLQGKDAAEVSLAQIKAY 292 +L+L++ KARQ L W R + L+ T WY L +D +S QI Y Sbjct: 302 -VLRLDTSKARQRLDWIPRMELYTALELTAQWYAGY-LNNEDLRALSENQIAFY 353 >gi|300313692|ref|YP_003777784.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 336 Score = 76.6 bits (187), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 84/290 (28%), Positives = 126/290 (43%), Gaps = 37/290 (12%) Query: 4 VEGDVRDLASLNKAVQSFKPEFVFHLAAQALVRESYSDPKSTFDTNVGGSVNLLEAVRAC 63 V+GD+R+ L ++ FK E V H A V ES P +D NV G+V LLEA++A Sbjct: 55 VKGDIRNREQLESVLREFKCEAVIHFAGLKAVGESVEQPLMYYDNNVVGTVRLLEAMQA- 113 Query: 64 DSVRSLVFVTSDKCYENVEWIWAYRENDALGGRDPYSASKAAAEIVFSSYLRSWFTTHPH 123 V+++VF +S Y + +++ E L +PY SK IV LR ++ HP Sbjct: 114 TGVKTIVFSSSATVYGDPQYL-PLDEAHPLSASNPYGRSK----IVIEDMLRDYYRAHPD 168 Query: 124 LGAASVRAGNVIG-------GGDWAADRIVPDCIRALVDKQAIVLR-----------NPA 165 A +R N +G G D +P+ + V + AI R P Sbjct: 169 WKIALLRYFNPVGAHESGLIGEDPGG---IPNNLMPFVAQVAIGRRERLNIWGGDYPTPD 225 Query: 166 AT--RPWQHVLEPISGYLKLAAYLYDAPHQYEGAWNFGPPVGDARTVLDVAKHLATQFVD 223 T R + HV++ G++ A DAP + A N G VG +VLDV K A + Sbjct: 226 GTGVRDFIHVVDLARGHVA-ALQALDAPQCF--AVNLGTGVG--YSVLDVLK--AYEKAC 278 Query: 224 GQVVVEASANKL-HEANLLQLNSDKARQMLQWSTRWDFEQTLQATGNWYQ 272 G+ + A A + + N +A++ L W E + W Q Sbjct: 279 GKTLPYAIAERRPGDIASCYANPAEAKRKLGWEATLGLEAMCADSWKWQQ 328 >gi|300311327|ref|YP_003775419.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 343 Score = 55.1 bits (131), Expect = 6e-09, Method: Compositional matrix adjust. Identities = 65/227 (28%), Positives = 96/227 (42%), Gaps = 27/227 (11%) Query: 4 VEGDVRDLASLNKAVQSFKPEFVFHLAAQALVRESYSDPKSTFDTNVGGSVNLLEAVRAC 63 V+GD+RD A L+ +S + E V H A V ES + P +D NV GS L EA+ A Sbjct: 58 VQGDIRDRAVLDGIFKSHRIEAVIHFAGLKAVGESVAQPLRYYDNNVHGSNVLFEAM-AA 116 Query: 64 DSVRSLVFVTSDKCYENVEWIWAYRENDALGGRDPYSASKAAAEIVFSSYLRSWFTTHPH 123 V+++VF +S Y + + E L +PY SK E + L P Sbjct: 117 HGVKNIVFSSSATVYGDPASV-PITEEFPLSATNPYGRSKLMVEQI----LGDLHVADPS 171 Query: 124 LGAASVRAGNVIG-------GGDWAA--DRIVP------DCIRALVDKQAIVLRNPAAT- 167 A +R N +G G D + + ++P D RA + P T Sbjct: 172 WRIALLRYFNPVGAHESGLIGEDPSGIPNNLLPFIAQVADGRRAALSVYGSDYPTPDGTG 231 Query: 168 -RPWQHVLEPISGYLKLAAYLYDAPHQYEGAWNFGPPVGDARTVLDV 213 R + HV++ G+LK L P Y +N G G +VL++ Sbjct: 232 VRDYIHVVDLALGHLKTLRKLEQGPGVY--TYNLG--TGRGNSVLEM 274 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.132 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 953,735 Number of extensions: 37757 Number of successful extensions: 100 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 100 Number of HSP's successfully gapped: 3 Length of query: 298 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 213 Effective length of database: 1,217,395 Effective search space: 259305135 Effective search space used: 259305135 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)