BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0217.p (396 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312062|ref|YP_003776154.1| membrane-anchored group 1 glyco... 79 6e-16 gi|300313501|ref|YP_003777593.1| glycosyl transferase [Herbaspir... 61 1e-10 gi|300311312|ref|YP_003775404.1| glycosyl transferase group 1 fa... 57 2e-09 >gi|300312062|ref|YP_003776154.1| membrane-anchored group 1 glycosyltransferase [Herbaspirillum seropedicae SmR1] Length = 406 Score = 79.0 bits (193), Expect = 6e-16, Method: Compositional matrix adjust. Identities = 65/214 (30%), Positives = 101/214 (47%), Gaps = 15/214 (7%) Query: 176 ASEIVTVSEAAAAALEQRNFR-KQIRVIHNGMQASRPSRQAAQAPA-----GDQARPLIG 229 A + T+ E A + R ++ VI N + S PA G + + L+G Sbjct: 161 ADAVTTICEGLRADIAARGVPLAKMTVIPNAVNIDDFSLGQQADPALARQLGLEGKTLLG 220 Query: 230 FLGALVPDKGAHVLLDALLRL---TPGGRVALAGPGQAEYTAHLRAHPATVTWEVSFVGE 286 F+G+ +G VL+ AL +L P R+ L G G E H A V+ +V F G Sbjct: 221 FIGSFYAYEGLPVLVQALPQLLAGNPDVRLLLVGGGPQERALHALAAELGVSDKVVFAGR 280 Query: 287 L--DDVSSFLQQIDILVVPSIRFEAFGMVI----LEAMEHCKPVICSDFGGMKEIVVNGE 340 + + V + ID+LV P ++ +V LEAM + V+ SD GG +E++ +G Sbjct: 281 VPHEQVQRYYNLIDVLVYPRLKMRLTDLVTPLKPLEAMAQGRLVVASDVGGHRELISDGR 340 Query: 341 TGMVVPAGDPDQLAAAIATLEADPALRKHMGTAG 374 TG++ AG P+ LA + L A+PA + AG Sbjct: 341 TGVLFAAGSPEALAQKVLALLAEPARWPQLRAAG 374 >gi|300313501|ref|YP_003777593.1| glycosyl transferase [Herbaspirillum seropedicae SmR1] Length = 400 Score = 61.2 bits (147), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 58/241 (24%), Positives = 105/241 (43%), Gaps = 27/241 (11%) Query: 176 ASEIVTVSEAAAAALEQRNFRKQIRVIHNGMQASRPSRQAAQAPAGDQARPLIGFLGALV 235 A +V + AA L R+ + ++HNG++ + A G + +GF+G L+ Sbjct: 153 ADAVVPIDHTVAATL-GRDL--SLEIVHNGLRVD-AGKHLDGAAQGARGTVRVGFMGVLI 208 Query: 236 PDKGAHVLLDALLRLTPGG---RVALAGPG-------------QAEYTAHLRA------H 273 KG + LL+A+ L G +AG + + ++RA H Sbjct: 209 ALKGIYELLEAMRILKNRGVAIECVIAGENARNLKGLRAWVLRKLGFARNVRADLEQMIH 268 Query: 274 PATVTWEVSFVGELDDVSSFLQQIDILVVPSIRFEAFGMVILEAMEHCKPVICSDFGGMK 333 + V +G + DV S +DIL PS +A G + EA + P + + + Sbjct: 269 DYGLEKHVQLLGFVKDVRSLYPTLDILCFPS-HLDAAGRPVFEAAFYSIPSVVAVKDPLP 327 Query: 334 EIVVNGETGMVVPAGDPDQLAAAIATLEADPALRKHMGTAGHARLKKCFTVERMAAQYAA 393 + V++ TG+ +P+ +P+ +A A+ L D LR+ +G F++E A+ A Sbjct: 328 DAVLHEHTGLAIPSPEPELIADALQRLAEDHVLRQTLGRQARDWAISNFSIESSASTMVA 387 Query: 394 L 394 + Sbjct: 388 I 388 >gi|300311312|ref|YP_003775404.1| glycosyl transferase group 1 family protein [Herbaspirillum seropedicae SmR1] Length = 419 Score = 57.0 bits (136), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 65/218 (29%), Positives = 102/218 (46%), Gaps = 22/218 (10%) Query: 190 LEQRNFRKQIRVIHNGMQASRPSRQAAQAPAGDQARPLIGFLGALVPDKGAHVLLDA--- 246 +EQ + K + ++H G++ + A AP Q + +G L KG +L++A Sbjct: 193 VEQSAWDK-VAIVHCGLERAF----YAGAPTTPQIALRLVCIGRLCEQKGQLLLVEAAAQ 247 Query: 247 LLRLTPGGRVALAGPGQ-----AEYTAH--LRAHPATVTWEVSFVGELDDVSSFLQQIDI 299 L+R + LAG G+ E AH L+ H W S V L Sbjct: 248 LMRRGVAFELVLAGDGEMRAQIEERIAHHDLQQHIRITGWISS-----AQVREELLAARA 302 Query: 300 LVVPSIRFEAFGMVILEAMEHCKPVICSDFGGMKEIVVNGETGMVVPAGDPDQLAAAI-A 358 +V+PS E +VI+EAM +PV+ + G+ E+V++ E G + PAGD ++L A+ A Sbjct: 303 MVLPSFA-EGLPVVIMEAMALRRPVLTTYIAGIPELVIHEENGWLFPAGDIERLTDAMQA 361 Query: 359 TLEADPALRKHMGTAGHARLKKCFTVERMAAQYAALLS 396 L+A P MG A R + +V AA+ A L + Sbjct: 362 CLQATPEQLAAMGQAAQLRAVERHSVNVEAAKLARLFN 399 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.138 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,273,412 Number of extensions: 54464 Number of successful extensions: 222 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 224 Number of HSP's successfully gapped: 5 Length of query: 396 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 309 Effective length of database: 1,207,925 Effective search space: 373248825 Effective search space used: 373248825 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 131 (55.1 bits)