BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0031.0187.p
         (261 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313465|ref|YP_003777557.1| penicillin-binding 1 (peptidogl...   350   5e-98
gi|300310721|ref|YP_003774813.1| penicillin-binding 1 (peptidogl...   166   2e-42
gi|300312242|ref|YP_003776334.1| penicillin-binding 2 protein [H...    60   2e-10
gi|300309743|ref|YP_003773835.1| monofunctional biosynthetic pep...    57   2e-09

>gi|300313465|ref|YP_003777557.1| penicillin-binding 1
           (peptidoglycan synthetase) transmembrane protein
           [Herbaspirillum seropedicae SmR1]
          Length = 771

 Score =  350 bits (899), Expect = 5e-98,   Method: Compositional matrix adjust.
 Identities = 169/169 (100%), Positives = 169/169 (100%)

Query: 1   MHWSLRILLGLVGTIFGLAVMVLLGVTFIVALTYPKLPDLDTLTDYRPKIPLRIFSADGV 60
           MHWSLRILLGLVGTIFGLAVMVLLGVTFIVALTYPKLPDLDTLTDYRPKIPLRIFSADGV
Sbjct: 1   MHWSLRILLGLVGTIFGLAVMVLLGVTFIVALTYPKLPDLDTLTDYRPKIPLRIFSADGV 60

Query: 61  LIGEFGEERRNLVHFKDIPEIMKKAVLAIEDDRFYEHGGVDYQGIARATFSNLSGGGGGA 120
           LIGEFGEERRNLVHFKDIPEIMKKAVLAIEDDRFYEHGGVDYQGIARATFSNLSGGGGGA
Sbjct: 61  LIGEFGEERRNLVHFKDIPEIMKKAVLAIEDDRFYEHGGVDYQGIARATFSNLSGGGGGA 120

Query: 121 STITQQVARNFFLTSNEPTFLQKATRKFFYEIPLAWKIENNLTKDQILE 169
           STITQQVARNFFLTSNEPTFLQKATRKFFYEIPLAWKIENNLTKDQILE
Sbjct: 121 STITQQVARNFFLTSNEPTFLQKATRKFFYEIPLAWKIENNLTKDQILE 169


>gi|300310721|ref|YP_003774813.1| penicillin-binding 1
           (peptidoglycan synthetase) transmembrane protein
           [Herbaspirillum seropedicae SmR1]
          Length = 822

 Score =  166 bits (420), Expect = 2e-42,   Method: Compositional matrix adjust.
 Identities = 92/158 (58%), Positives = 111/158 (70%), Gaps = 8/158 (5%)

Query: 14  TIFGLAVMVLLGVTFIVALTYPKLPDLDTLTDYRPKIPLRIFSADGVLIGEFGEERRNLV 73
           +I GL V   L V + +    P+LP L+ +TDYRPK+PLRI+S D VL+GEFGEE+R+LV
Sbjct: 32  SILGLGVAGALLVVYALIFINPRLPSLELITDYRPKVPLRIWSEDKVLLGEFGEEKRSLV 91

Query: 74  HFKDIPEIMKKAVLAIEDDRFYEHGGVDYQGIARATFSNLSGGGG--GASTITQQVARNF 131
              DIP  MK AVLAIED RFYEHGG+D+ GIARA  +++  G    GASTIT QVARN 
Sbjct: 92  KLDDIPADMKNAVLAIEDYRFYEHGGIDWIGIARALVTDVLRGSASQGASTITMQVARNV 151

Query: 132 FLTSNEPTFLQKATRKFFYEIPLAWKIENNLTKDQILE 169
           FLT  E TF +K      YE+ LA KIE+ LTKDQILE
Sbjct: 152 FLTK-EKTFSRK-----LYEVLLANKIEHALTKDQILE 183


>gi|300312242|ref|YP_003776334.1| penicillin-binding 2 protein
           [Herbaspirillum seropedicae SmR1]
          Length = 753

 Score = 59.7 bits (143), Expect = 2e-10,   Method: Compositional matrix adjust.
 Identities = 35/110 (31%), Positives = 56/110 (50%), Gaps = 8/110 (7%)

Query: 65  FGEERRNLVHFKDIPEIMKKAVLAIEDDRFYEHGGVDYQGIARATFSNL-SGGGGGASTI 123
             E +   V  +D+   ++ A++A ED RFY+H G+D+  +A + + NL +    GASTI
Sbjct: 59  MDERKLAWVRLEDVSPALRHALIASEDRRFYQHSGIDWSAVAASAWGNLWNSKTRGASTI 118

Query: 124 TQQVARNFFLTSNEPTFLQKATRKFFYEIPLAW---KIENNLTKDQILEG 170
           T Q+A   +    +    +   R    ++  AW    +E N  KDQILE 
Sbjct: 119 TMQLAGLIY----DDLRRKSGARSLTQKVSQAWIAQSLERNWRKDQILEA 164


>gi|300309743|ref|YP_003773835.1| monofunctional biosynthetic
           peptidoglycan transglycosylase [Herbaspirillum
           seropedicae SmR1]
          Length = 235

 Score = 57.0 bits (136), Expect = 2e-09,   Method: Compositional matrix adjust.
 Identities = 36/99 (36%), Positives = 54/99 (54%), Gaps = 8/99 (8%)

Query: 73  VHFKDIPEIMKKAVLAIEDDRFYEHGGVDYQGIARATFSNLSGGG--GGASTITQQVARN 130
           V +  I   +K+A++A ED  F  H GVD++ + +A   N   G    G STITQQ+A+N
Sbjct: 65  VPYARISNNLKRAIIASEDSNFSSHDGVDWEALQKAYEKNEKKGKVVAGGSTITQQLAKN 124

Query: 131 FFLTSNEPTFLQKATRKFFYEIPLAWKIENNLTKDQILE 169
            FL S E ++L+K       E  + + +E  L K +I E
Sbjct: 125 LFL-SGERSYLRKGQ-----EFIITYMLEFLLDKQRIFE 157


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.324    0.141    0.431 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 816,597
Number of extensions: 32650
Number of successful extensions: 110
Number of sequences better than 1.0e-08: 4
Number of HSP's gapped: 107
Number of HSP's successfully gapped: 4
Length of query: 261
Length of database: 1,619,870
Length adjustment: 83
Effective length of query: 178
Effective length of database: 1,226,865
Effective search space: 218381970
Effective search space used: 218381970
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 129 (54.3 bits)