BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0186.p (505 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313465|ref|YP_003777557.1| penicillin-binding 1 (peptidogl... 1037 0.0 gi|300310721|ref|YP_003774813.1| penicillin-binding 1 (peptidogl... 565 e-162 gi|300312242|ref|YP_003776334.1| penicillin-binding 2 protein [H... 99 1e-21 gi|300309672|ref|YP_003773764.1| cell division FtsI protein/peni... 57 5e-09 >gi|300313465|ref|YP_003777557.1| penicillin-binding 1 (peptidoglycan synthetase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 771 Score = 1037 bits (2682), Expect = 0.0, Method: Compositional matrix adjust. Identities = 503/505 (99%), Positives = 505/505 (100%) Query: 1 VHAEYVAEMARQLVYAQYKDETYTRGLNVFTTITKTDQDAAYIALRRGIIDYERRHGYRG 60 +HAEYVAEMARQLVYAQYKDETYTRGLNVFTTITKTDQDAAYIALRRGIIDYERRHGYRG Sbjct: 267 MHAEYVAEMARQLVYAQYKDETYTRGLNVFTTITKTDQDAAYIALRRGIIDYERRHGYRG 326 Query: 61 PEGYMDLPENATKDAVEDAVEVELADHPDSDDMVSAVVLEAKPKEITAMLSTGEEISISG 120 PEGYMDLPENATKDAVEDAVEVELADHPDSDDMVSAVVLEAKPKEITAMLSTGEEISISG Sbjct: 327 PEGYMDLPENATKDAVEDAVEVELADHPDSDDMVSAVVLEAKPKEITAMLSTGEEISISG 386 Query: 121 AGLGFGANLLSDKAPPQKKIRRGAIIRVFQDGNNWIITQMPEVESAFVSVNSQDGAIRSL 180 AGLGFGANLLSDKAPPQKKIRRGAIIRVFQDGNNWIITQMPEVESAFVSVNSQDGAIRSL Sbjct: 387 AGLGFGANLLSDKAPPQKKIRRGAIIRVFQDGNNWIITQMPEVESAFVSVNSQDGAIRSL 446 Query: 181 VGGFDFNRNKFNHVTQAWRQPGSSFKPFIYSSSLEKGLSPATIINDAPLTFTQTGGQVWQ 240 VGGFDFNRNKFNHVTQAWRQPGSSFKPFIYSSSLEKGLSPATIINDAPLTFTQTGGQVWQ Sbjct: 447 VGGFDFNRNKFNHVTQAWRQPGSSFKPFIYSSSLEKGLSPATIINDAPLTFTQTGGQVWQ 506 Query: 241 PKNYGGKFEGPISMRRALQKSINLVSIRILERVGVKYAQEYITRFGFDADKNPPYLTLAL 300 PKNYGGKFEGPISMRRALQKSINLVSIRILERVGVKYAQEYITRFGFDADKNPPYLTLAL Sbjct: 507 PKNYGGKFEGPISMRRALQKSINLVSIRILERVGVKYAQEYITRFGFDADKNPPYLTLAL 566 Query: 301 GAGNVTPLQMVGAYSVFANGGYKINPYLITKVTDSNGEVLSQAKPATADDESNRVIDARN 360 GAGNVTPLQMVGAYSVFANGGYKINPYLITKVTDSNGEVLSQAKPATADDESNRVIDARN Sbjct: 567 GAGNVTPLQMVGAYSVFANGGYKINPYLITKVTDSNGEVLSQAKPATADDESNRVIDARN 626 Query: 361 AFIMDSMLRDVVRHGTAVKALSLKRTDLAGKTGTTNDSMDAWFAGYQPGLTAIAWIGYDQ 420 AFIMDSMLRDVVRHGTAVKALSLKRTDLAGKTGTTNDSMDAWFAGYQPGLTAIAWIGYDQ Sbjct: 627 AFIMDSMLRDVVRHGTAVKALSLKRTDLAGKTGTTNDSMDAWFAGYQPGLTAIAWIGYDQ 686 Query: 421 PKSLGDRETGGGLALPVWISYMSKALKDVPNVERKVPDGLIESGGEYYYAEFPPSAMVRD 480 PKSLGDRETGGGLALPVWISYMSKALKDVPNV+RKVPDGLIESGGEYYYAEFPPSAMVRD Sbjct: 687 PKSLGDRETGGGLALPVWISYMSKALKDVPNVDRKVPDGLIESGGEYYYAEFPPSAMVRD 746 Query: 481 LGMGDRSGLTPEEEKAKTQMKNELF 505 LGMGDRSGLTPEEEKAKTQMKNELF Sbjct: 747 LGMGDRSGLTPEEEKAKTQMKNELF 771 >gi|300310721|ref|YP_003774813.1| penicillin-binding 1 (peptidoglycan synthetase) transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 822 Score = 565 bits (1455), Expect = e-162, Method: Compositional matrix adjust. Identities = 271/487 (55%), Positives = 369/487 (75%), Gaps = 7/487 (1%) Query: 1 VHAEYVAEMARQLVYAQYKDETYTRGLNVFTTITKTDQDAAYIALRRGIIDYERRHGYRG 60 +HAEYV EM RQ+VYAQYK++TYT+G NV+TT+ DQ AAY+A+R+G++DY+RRHGYRG Sbjct: 281 IHAEYVNEMVRQMVYAQYKEDTYTQGFNVYTTVNAADQQAAYLAVRKGVMDYDRRHGYRG 340 Query: 61 PEGYMDLPENATKDAVEDAVEVELADHPDSDDMVSAVVLEAKPKEITAMLSTGEEISISG 120 PE ++LP NA A A++ + DHPD+DD+++AVV+ A PK+I A+L +G++ISI G Sbjct: 341 PEETIELPANAEDRAA--AIDDVMDDHPDNDDILAAVVVAASPKQIDAVLRSGDKISIKG 398 Query: 121 AGLGFGANLLSDKAPPQKKIRRGAIIRVFQDG-NNWIITQMPEVESAFVSVNSQDGAIRS 179 L F A+ LS+KA +I GA+IRV QD NW + Q+P+VE+A VSV QDGAIR+ Sbjct: 399 EALRFVASALSEKAKKDIRIGPGALIRVMQDAKQNWQVAQLPQVEAALVSVVPQDGAIRA 458 Query: 180 LVGGFDFNRNKFNHVTQAWRQPGSSFKPFIYSSSLEKGLSPATIINDAPLTFTQTGGQV- 238 LVGGFDF +N FNHVTQAWRQPGS+FKPFIYS++LEKG +PA+I+NDAP+++ GQ Sbjct: 459 LVGGFDFTQNNFNHVTQAWRQPGSTFKPFIYSAALEKGFAPASIVNDAPVSYPAGPGQPN 518 Query: 239 WQPKNYGGKFEGPISMRRALQKSINLVSIRILERVGVKYAQEYIT-RFGFDADKNPPYLT 297 W+P++ GPI++R ALQKS+NLV+IR L+ +GV YA+++IT +GFDADK PPYL Sbjct: 519 WEPRD-DETPAGPITVRTALQKSVNLVAIRTLDYIGVPYAKDFITGTYGFDADKVPPYLP 577 Query: 298 LALGAGNVTPLQMVGAYSVFANGGYKINPYLITKVTDSNGEVLSQAKPATADDESNRVID 357 +ALGAG PLQ+ AYSVFANGGYK+ PYLI+++TD+ G+ LS+A+P + + RV+ Sbjct: 578 MALGAGQTNPLQLSAAYSVFANGGYKVQPYLISQITDARGQALSKAEPLIVGNGAPRVVS 637 Query: 358 ARNAFIMDSMLRDVVRHGTAVKALSLKRTDLAGKTGTTNDSMDAWFAGYQPGLTAIAWIG 417 RN+++M SML+ V + GT ++ S+ R+DLAGKTGTTND++D WFAGYQ L + W+G Sbjct: 638 ERNSYMMTSMLKSVAQGGTGARSNSIGRSDLAGKTGTTNDAVDGWFAGYQRTLATVVWMG 697 Query: 418 YDQPKSLGDRETGGGLALPVWISYMSKALKDVPNVERKVPDGLIESGGEYYYAEFPP-SA 476 YDQPKSLG +E G LALP+W+ YM+KALK VP + VP+G+ GE YY F P + Sbjct: 698 YDQPKSLGSKEFGAQLALPIWVDYMAKALKGVPQFDLPVPEGIRFIDGEPYYDNFTPNNG 757 Query: 477 MVRDLGM 483 +V ++G+ Sbjct: 758 LVTNVGV 764 >gi|300312242|ref|YP_003776334.1| penicillin-binding 2 protein [Herbaspirillum seropedicae SmR1] Length = 753 Score = 98.6 bits (244), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 81/252 (32%), Positives = 129/252 (51%), Gaps = 22/252 (8%) Query: 197 AWRQPGSSFKPFIYSSSLEKG-LSPATIINDAPLTFTQTGGQVWQPKNYGGKFEGPISMR 255 A RQ GS+ KPF+Y ++EK L+ A++++D+P+ + G ++ P+NY +++G +S+R Sbjct: 341 AQRQAGSTLKPFLYELAIEKKWLTAASLLDDSPVNLATSAG-LYIPQNYDKQYKGLVSLR 399 Query: 256 RALQKSINLVSIRILERVGVKYAQEYITRFGFDADKNPPY--LTLALGAGNVTPLQMVGA 313 +L S+N+ ++R L V + + GF+ ++ Y +LALG+ +VT + A Sbjct: 400 TSLGSSLNIPAVRTLVMVTPERFFRRLQALGFNLRESGDYYGYSLALGSADVTLANLANA 459 Query: 314 YSVFANGGYKINPYLITKVTDSNGEVLSQAKPATADDESNRVIDARNAFIMDSMLRD--- 370 Y V AN G + P L T++ ++ PA A +V+DA A+I+ +L D Sbjct: 460 YRVLANQG-RYTP-LRTRLDEAR-------TPARA----TQVMDADAAYIVGDILSDRSA 506 Query: 371 VVRHGTAVKALSLKRTDLAGKTGTTNDSMDAWFAGYQPGLTAIAWIGYDQPKSLGDRETG 430 R ALS R A KTGT+ D D W GY T W+G + D +G Sbjct: 507 RARTFGLENALS-TRIWTAVKTGTSKDMRDNWCVGYSTRYTVGVWVGNASGAPMWD-VSG 564 Query: 431 GGLALPVWISYM 442 A PVW M Sbjct: 565 VTGAAPVWQEVM 576 >gi|300309672|ref|YP_003773764.1| cell division FtsI protein/penicillin-binding protein 2 [Herbaspirillum seropedicae SmR1] Length = 592 Score = 56.6 bits (135), Expect = 5e-09, Method: Compositional matrix adjust. Identities = 75/287 (26%), Positives = 117/287 (40%), Gaps = 54/287 (18%) Query: 192 NHVTQAWRQPGSSFKPFIYSSSLEKG-LSPATIINDAPLTFTQTGGQVWQPKNYGGKFEG 250 N V +PGS+ KPF + +L+ ++P+T+ AP T + +G Sbjct: 305 NRVVTDTFEPGSTMKPFTVALALDTHRVTPSTVFQTAPGKMTIGTATIGDSHAHG----- 359 Query: 251 PISMRRALQKSINLVSIRILERVGVKYAQEYITRFGFD-----------ADKNPPY---- 295 P+++ + ++KS N+ + +I + + E T GF A + PY Sbjct: 360 PLTVAQIIEKSSNIGTAKIALGMPPEEMWEMFTTVGFGQQPKFGFPGAVAGRVRPYKSWR 419 Query: 296 ----LTLALGAG-NVTPLQMVGAYSVFANGGYKINPYLITKVTDSNGEVLSQAKPATADD 350 T++ G G +V+ +Q+ AY +FA G I P TKVTDS Sbjct: 420 PIEQATMSYGHGISVSLIQLAHAYLIFARNG-DIIPLSFTKVTDS--------------P 464 Query: 351 ESNRVIDARNAFIMDSMLRDVVRHGTAVKALSLKRTDLAGKTGTTND--------SMDAW 402 RVI A A M ML VV G + + GKTGT A Sbjct: 465 IGQRVISADTALQMRRMLETVVAPGGTAPQAQVPGYRVGGKTGTAYKIEGGKYVRKYVAS 524 Query: 403 FAGYQP--GLTAIAWIGYDQPKSLGDRETGGGLALPVWISYMSKALK 447 F+G P I + D+P+ + GG +A PV+ + + AL+ Sbjct: 525 FSGIAPMSDPRLIISVMIDEPQG---QHYGGPVAGPVFANVAANALR 568 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.134 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,693,038 Number of extensions: 75481 Number of successful extensions: 214 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 210 Number of HSP's successfully gapped: 4 Length of query: 505 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 416 Effective length of database: 1,198,455 Effective search space: 498557280 Effective search space used: 498557280 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 133 (55.8 bits)