BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0150.p (459 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313431|ref|YP_003777523.1| zinc protease [Herbaspirillum s... 936 0.0 gi|300312757|ref|YP_003776849.1| Zn-dependent peptidase [Herbasp... 140 2e-34 gi|300313429|ref|YP_003777521.1| zinc protease-like signal pepti... 64 2e-11 >gi|300313431|ref|YP_003777523.1| zinc protease [Herbaspirillum seropedicae SmR1] Length = 459 Score = 936 bits (2419), Expect = 0.0, Method: Compositional matrix adjust. Identities = 458/459 (99%), Positives = 458/459 (99%) Query: 1 MKLKIRALLASALLGLFASSPMLAQAEPARQFVLGNGMNVIVKEDRRAPTAVQMVWYKVG 60 MKLKIRALLASALLGLFASSPMLAQAEPARQFVLGNGM VIVKEDRRAPTAVQMVWYKVG Sbjct: 1 MKLKIRALLASALLGLFASSPMLAQAEPARQFVLGNGMKVIVKEDRRAPTAVQMVWYKVG 60 Query: 61 GIDEVNGLTGVSHALEHMMFKGTRNHKVGEFSRLVAELGGQENAFTANDFTAYFQQIEKS 120 GIDEVNGLTGVSHALEHMMFKGTRNHKVGEFSRLVAELGGQENAFTANDFTAYFQQIEKS Sbjct: 61 GIDEVNGLTGVSHALEHMMFKGTRNHKVGEFSRLVAELGGQENAFTANDFTAYFQQIEKS 120 Query: 121 HLEKVMALEADRMANLQFDAAEFAKEIRVIMEERRWRTDDQPMGLLNEALNAAAWTAHPY 180 HLEKVMALEADRMANLQFDAAEFAKEIRVIMEERRWRTDDQPMGLLNEALNAAAWTAHPY Sbjct: 121 HLEKVMALEADRMANLQFDAAEFAKEIRVIMEERRWRTDDQPMGLLNEALNAAAWTAHPY 180 Query: 181 HHPVVGWMDDLQHMSVQDIAAWYRQWYAPNNATLVVAGDVDAQRVLALARKYFGKIPARR 240 HHPVVGWMDDLQHMSVQDIAAWYRQWYAPNNATLVVAGDVDAQRVLALARKYFGKIPARR Sbjct: 181 HHPVVGWMDDLQHMSVQDIAAWYRQWYAPNNATLVVAGDVDAQRVLALARKYFGKIPARR 240 Query: 241 LPAGKPQNEPQQLGMRRVTVKAPAENPYVVMAWKTPALRQVEQDQDVYALDVLSAVLDGY 300 LPAGKPQNEPQQLGMRRVTVKAPAENPYVVMAWKTPALRQVEQDQDVYALDVLSAVLDGY Sbjct: 241 LPAGKPQNEPQQLGMRRVTVKAPAENPYVVMAWKTPALRQVEQDQDVYALDVLSAVLDGY 300 Query: 301 DNARLSASLVRTGGKATAVGASYSGVARGPVLFTLEGSPAEGVTTAQLEGLLRGEVERIA 360 DNARLSASLVRTGGKATAVGASYSGVARGPVLFTLEGSPAEGVTTAQLEGLLRGEVERIA Sbjct: 301 DNARLSASLVRTGGKATAVGASYSGVARGPVLFTLEGSPAEGVTTAQLEGLLRGEVERIA 360 Query: 361 REGVSEQELQRVKTQLIASQVYKRDSVFGQAMEIGMMETAGLGQQNIDRIIERLKSVTAA 420 REGVSEQELQRVKTQLIASQVYKRDSVFGQAMEIGMMETAGLGQQNIDRIIERLKSVTAA Sbjct: 361 REGVSEQELQRVKTQLIASQVYKRDSVFGQAMEIGMMETAGLGQQNIDRIIERLKSVTAA 420 Query: 421 QVQAVAQQYFQDDTLTVATLVPLPLAGKKPAPPPAGLLH 459 QVQAVAQQYFQDDTLTVATLVPLPLAGKKPAPPPAGLLH Sbjct: 421 QVQAVAQQYFQDDTLTVATLVPLPLAGKKPAPPPAGLLH 459 >gi|300312757|ref|YP_003776849.1| Zn-dependent peptidase [Herbaspirillum seropedicae SmR1] Length = 929 Score = 140 bits (354), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 113/425 (26%), Positives = 199/425 (46%), Gaps = 23/425 (5%) Query: 26 AEPARQFVLGNGMNVIVKEDRRAPTAVQMVWYKVGGIDEVNGLTGVSHALEHMMFKGTRN 85 AE ++ NG +++ D PT + Y VG E G TG++H LEH+MFKG+ Sbjct: 57 AEGISEYRFANGFKLLLLPDDSQPTVTVNITYLVGSRHENYGETGMAHLLEHLMFKGSPR 116 Query: 86 HKVGEFSRLVAELGGQENAFTANDFTAYFQQIEKS--HLEKVMALEADRMANLQFDAAEF 143 H + ++ G N T D T Y++ + S +L +A+EADRM N + + Sbjct: 117 HP--SIPQDFSKRGMNFNGTTWLDRTNYYETFQASPDNLRWAIAMEADRMLNSKIARKDL 174 Query: 144 AKEIRVIMEERRWRTDDQPMGLLNEALNAAAWTAHPYHHPVVGWMDDLQHMSVQDIAAWY 203 E+ V+ E + P ++ + + + A+ H Y +G D++++ ++++ A+Y Sbjct: 175 DSEMTVVRNEFE-AGETSPTRVMLKRMQSVAYDWHAYGRNTIGARSDIENVRIENLQAFY 233 Query: 204 RQWYAPNNATLVVAGDVDAQRVLALARKYFGKI--PARRLPAGKPQNEPQQLGMRRVTVK 261 R +Y P+NA L++AG DA +VL + FG++ P R LPA EP Q G R+ ++ Sbjct: 234 RTYYQPDNAVLLIAGKFDAAQVLQWVDQSFGRLPKPKRTLPAFW-TVEPTQDGERQFVIR 292 Query: 262 APAENPYVVMAWKTPALRQVEQDQDVYALDVLSAVLDGYDNARLSASLVRTGGKATAVGA 321 + V +A+K P+ D AL + +L N RL +LV TG Sbjct: 293 RRGDQQLVALAYKMPSALH----PDATALGFAADILTDTPNGRLHKALVETGMATEVYAM 348 Query: 322 SYSGVARGPVLFTLE---GSPAEGVTTAQLEGLLRGEVERIAREGVSEQELQRVKTQLIA 378 G+A G + + G + V A + VE + + + +E+ R+ + A Sbjct: 349 PLEGMAPGLQMMVAKVKVGGDLDAVRQAMISA-----VESFSTQPPTPEEVARLHRE--A 401 Query: 379 SQVYKRDSVFGQAMEIGMMETAGLGQQNIDRI-IERLKSVTAAQVQAVAQQYFQDDTLTV 437 + ++ Q + + M G + I +R++ +++A + A A +YF+ D TV Sbjct: 402 ANSFETLQNNPQQLAVAMSNAIARGDWRLLFIERDRMQRLSSADIAAAAGRYFRRDNRTV 461 Query: 438 ATLVP 442 +P Sbjct: 462 GLYLP 466 Score = 56.6 bits (135), Expect = 5e-09, Method: Compositional matrix adjust. Identities = 98/424 (23%), Positives = 177/424 (41%), Gaps = 61/424 (14%) Query: 41 IVKEDRRAPTAVQMVWYKVGGIDEVNGLTGVSHALEHMMFKGT----RNHKVGEFSRLVA 96 ++ + R T + + G + G V+ E M+ +GT R EF+RL Sbjct: 528 LLPKQSRGQTVSVSLHLRFGDAQSLFGQRNVAQLTEAMLMRGTDKLDRRQLADEFARL-- 585 Query: 97 ELGGQENAFTANDFTAYFQQIEKSHLEKVMALEADRMANLQFDAAEFAK---EIRVIMEE 153 ++ G + Y Q + +L +AL + + + DAAEF + E +E Sbjct: 586 KING----------SLYRFQTTRENLVPAIALVGEVFRHPRMDAAEFEQLKNETVAGLEA 635 Query: 154 RRWRTDDQPMGLLNEALNAAAWTAHPYHHPVVGW---------MDDLQHMSVQDIAAWYR 204 R P EAL + H H+P W + ++ ++++ + ++R Sbjct: 636 GR----KDPDTRAGEAL-----SVHFDHYPAGDWRAAQTLEQRIAAVRAVTLEQVKDFHR 686 Query: 205 QWYAPNNATLVVAGDVD-AQRVLALARKYFGKIPARRLPAGKPQNEPQQLGMRRVTVKAP 263 ++Y + L V GD D AQ A+ + + G A P + + +R+ V P Sbjct: 687 RFYGASQGELAVVGDFDPAQARSAIDQAFGGWTSA--APYARVLQTWADIPAKRLLVDTP 744 Query: 264 AENPYVVMAWKTPALRQVEQDQDVYALDVLSAVLDGYD-NARLSASLVRTGGKATAVGAS 322 + V A + +R + D D AL V + +L G +RL+ + + G + + Sbjct: 745 DKENGVYFARQN--IRLSDTDPDYPALAVANFLLGGSSLKSRLADRVRQQDGLSYGI--- 799 Query: 323 YSGVA------RGPVLFTLEGSPAEGVTTAQLEGLLRGEVERIAREGVSEQELQRVKTQL 376 YSG+ G + +P +++ +R E+ ++G SE+ELQR K+ + Sbjct: 800 YSGLQVAALTDAGHLTIYAIAAPQ---NLDKVDAAVREEIALALKDGFSEEELQRAKSGI 856 Query: 377 IASQVYKR---DSVFGQAMEIGMMETAGLGQQNIDRIIERLKSVTAAQVQAVAQQYFQDD 433 + + +R D+V + + L QQ IDR +L S+T AQV A ++Y Sbjct: 857 LQQRNQQRAGNDNVAATWTLLMNQDRNFLWQQEIDR---KLASLTLAQVNAALRKYVDPA 913 Query: 434 TLTV 437 +T Sbjct: 914 RMTT 917 >gi|300313429|ref|YP_003777521.1| zinc protease-like signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 438 Score = 64.3 bits (155), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 69/284 (24%), Positives = 135/284 (47%), Gaps = 23/284 (8%) Query: 161 QPMGLLNEALNAAAWTAHPYHHPVVGWMD----DLQHMSVQDIAAWYRQWYAPNNATLVV 216 +P + +A AA+ +HPY MD +Q ++ +D+ A++R Y N A + + Sbjct: 163 KPEVIAEKAFMHAAYGSHPY------AMDASEASMQAITREDLQAFHRAHYVANRAVIAL 216 Query: 217 AGDVDAQRVLALARKYFGKIP-ARRLPAGKPQNEPQQLGMRRVTVKAPAENPYVVMAWKT 275 GD++ ++ A+A ++P LPA P P+ R + PA ++++ Sbjct: 217 IGDINLEQARAIASALTRELPQGAALPALPPVVAPKGSEER---IAHPASQSHILIG--A 271 Query: 276 PALRQVEQDQDVYALDVLSAVLDGYDN-ARLSASLVRTGGKATAVGASYSGVAR-GPVLF 333 PA+++ D D +AL V + VL G +RL+ + G + +V + +S +A+ GP Sbjct: 272 PAIQR--GDPDFFALTVGNYVLGGGGFVSRLTDEVREKRGLSYSVYSGFSPLAQPGPFQI 329 Query: 334 TLEGSPAEGVTTAQLEGLLRGEVERIAREGVSEQELQRVKTQLIASQVYKRDSVFGQAME 393 L+ + TA+ + R +++ +EG + EL+ K L + DS Sbjct: 330 GLQTKKEQ---TAEALRVTRVTLDKFMQEGPTAAELKAAKDNLAGGFALRIDSNAKLLEN 386 Query: 394 IGMMETAGLGQQNIDRIIERLKSVTAAQVQAVAQQYFQDDTLTV 437 + ++ GL +D IER+++V+ V+A +++ + L+ Sbjct: 387 LSVIGFYGLPLDYLDHWIERIRAVSVQDVRAAFRKHVHPEELST 430 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.132 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,290,845 Number of extensions: 56334 Number of successful extensions: 189 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 186 Number of HSP's successfully gapped: 4 Length of query: 459 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 371 Effective length of database: 1,203,190 Effective search space: 446383490 Effective search space used: 446383490 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)