BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0148.p (438 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313429|ref|YP_003777521.1| zinc protease-like signal pepti... 870 0.0 gi|300312757|ref|YP_003776849.1| Zn-dependent peptidase [Herbasp... 93 5e-20 gi|300313431|ref|YP_003777523.1| zinc protease [Herbaspirillum s... 62 1e-10 >gi|300313429|ref|YP_003777521.1| zinc protease-like signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 438 Score = 870 bits (2247), Expect = 0.0, Method: Compositional matrix adjust. Identities = 432/438 (98%), Positives = 434/438 (99%) Query: 1 MKKFLCLLILPLGLLTSLPAPAALAIKSWTQPDGARVLFVPNHAIPMLDVSVQFDAGQRR 60 MKKFLCLLILPLGLL SLPA AALAI+SWTQPDGARVLFVPNHAIPMLDVSVQFDAGQRR Sbjct: 1 MKKFLCLLILPLGLLASLPAAAALAIQSWTQPDGARVLFVPNHAIPMLDVSVQFDAGQRR 60 Query: 61 DPAGKAGLAELTVASLTRGVTDASGTLTEAQILDGFADVAAQQHDGAGQDRAGVSLRTLS 120 DPAGKAGLAELTVASLTRGVTDASGTLTEAQILDGFADVAAQQHDGAGQDRAGVSLRTLS Sbjct: 61 DPAGKAGLAELTVASLTRGVTDASGTLTEAQILDGFADVAAQQHDGAGQDRAGVSLRTLS 120 Query: 121 SPAEREAALTLLARLLAHPSFPQASLERDRALAIANIKEELTKPEVIAEKAFMHAAYGSH 180 SPAEREAALTLLAR+LAHPSFPQASLERDRALAIANIKEELTKPEVIAEKAFMHAAYGSH Sbjct: 121 SPAEREAALTLLARMLAHPSFPQASLERDRALAIANIKEELTKPEVIAEKAFMHAAYGSH 180 Query: 181 PYAMDASEASMQAITREDLQAFHRAHYVANRAVIALIGDINLEQARAIASALTRELPQGA 240 PYAMDASEASMQAITREDLQAFHRAHYVANRAVIALIGDINLEQARAIASALTRELPQGA Sbjct: 181 PYAMDASEASMQAITREDLQAFHRAHYVANRAVIALIGDINLEQARAIASALTRELPQGA 240 Query: 241 ALPALPPVGAPKGSEERIAHPASQSHILIGAPAIQRGDPDFFALTVGNYVLGGGGFVSRL 300 ALPALPPV APKGSEERIAHPASQSHILIGAPAIQRGDPDFFALTVGNYVLGGGGFVSRL Sbjct: 241 ALPALPPVVAPKGSEERIAHPASQSHILIGAPAIQRGDPDFFALTVGNYVLGGGGFVSRL 300 Query: 301 TDEVREKRGLSYSVYSGFSPLAQPGPFQIGLQTKKEQTAEALRVTRATLDKFMQEGPTAA 360 TDEVREKRGLSYSVYSGFSPLAQPGPFQIGLQTKKEQTAEALRVTR TLDKFMQEGPTAA Sbjct: 301 TDEVREKRGLSYSVYSGFSPLAQPGPFQIGLQTKKEQTAEALRVTRVTLDKFMQEGPTAA 360 Query: 361 ELKAAKDNLAGGFALRIDSNAKLLENLSVIGFYGLPLDYLDHWIERIRAVSVQDVRAAFR 420 ELKAAKDNLAGGFALRIDSNAKLLENLSVIGFYGLPLDYLDHWIERIRAVSVQDVRAAFR Sbjct: 361 ELKAAKDNLAGGFALRIDSNAKLLENLSVIGFYGLPLDYLDHWIERIRAVSVQDVRAAFR 420 Query: 421 KHVHPEELSTIIVGESAP 438 KHVHPEELSTIIVGESAP Sbjct: 421 KHVHPEELSTIIVGESAP 438 >gi|300312757|ref|YP_003776849.1| Zn-dependent peptidase [Herbaspirillum seropedicae SmR1] Length = 929 Score = 92.8 bits (229), Expect = 5e-20, Method: Compositional matrix adjust. Identities = 102/413 (24%), Positives = 175/413 (42%), Gaps = 59/413 (14%) Query: 48 LDVSVQFDAGQRRDPAGKAGLAELTVASLTRGVTDASGTLTEAQILDGFADVAAQQHDGA 107 + VS+ G + G+ +A+LT A L RG + L Q+ D FA + Sbjct: 538 VSVSLHLRFGDAQSLFGQRNVAQLTEAMLMRG----TDKLDRRQLADEFARLKIN----- 588 Query: 108 GQDRAGVSLRTLSSPAEREAALTLLARLLAHPSFPQASLERDRALAIANIKEELTKPEVI 167 G R ++ A+ L+ + HP A E+ + +A ++ P+ Sbjct: 589 -----GSLYRFQTTRENLVPAIALVGEVFRHPRMDAAEFEQLKNETVAGLEAGRKDPDTR 643 Query: 168 AEKA----FMHAAYGSHPYA--MDASEASMQAITREDLQAFHRAHYVANRAVIALIGDIN 221 A +A F H G A ++ A+++A+T E ++ FHR Y A++ +A++GD + Sbjct: 644 AGEALSVHFDHYPAGDWRAAQTLEQRIAAVRAVTLEQVKDFHRRFYGASQGELAVVGDFD 703 Query: 222 LEQAR-AIASAL---TRELPQGAALPALPPVGAPKGSEERIAHPASQSHILIGAPAIQRG 277 QAR AI A T P L + A + + P ++ + I+ Sbjct: 704 PAQARSAIDQAFGGWTSAAPYARVLQTWADIPAKR---LLVDTPDKENGVYFARQNIRLS 760 Query: 278 D--PDFFALTVGNYVLGGGGFVSRLTDEVREKRGLSYSVYSGF--SPLAQPGPFQIGLQT 333 D PD+ AL V N++LGG SRL D VR++ GLSY +YSG + L G I Sbjct: 761 DTDPDYPALAVANFLLGGSSLKSRLADRVRQQDGLSYGIYSGLQVAALTDAGHLTIYAIA 820 Query: 334 KKEQTAEALRVTRATLDKFMQEGPTAAELKAAK--------------DNLAGGFALRIDS 379 + + R + +++G + EL+ AK DN+A + L ++ Sbjct: 821 APQNLDKVDAAVREEIALALKDGFSEEELQRAKSGILQQRNQQRAGNDNVAATWTLLMNQ 880 Query: 380 NAKLLENLSVIGFYGLPLDYLDHWIERIRAVSVQDVRAAFRKHVHPEELSTII 432 + L + ++ ++++ V AA RK+V P ++T+I Sbjct: 881 DRNFLWQQEI--------------DRKLASLTLAQVNAALRKYVDPARMTTVI 919 >gi|300313431|ref|YP_003777523.1| zinc protease [Herbaspirillum seropedicae SmR1] Length = 459 Score = 62.0 bits (149), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 66/265 (24%), Positives = 126/265 (47%), Gaps = 23/265 (8%) Query: 163 KPEVIAEKAFMHAAYGSHPY------AMDASEASMQAITREDLQAFHRAHYVANRAVIAL 216 +P + +A AA+ +HPY MD +Q ++ +D+ A++R Y N A + + Sbjct: 161 QPMGLLNEALNAAAWTAHPYHHPVVGWMD----DLQHMSVQDIAAWYRQWYAPNNATLVV 216 Query: 217 IGDINLEQARAIASALTRELPQGAALPALPPVGAPKGSEER---IAHPASQSHILIG--A 271 GD++ ++ A+A ++P LPA P P+ R + PA ++++ Sbjct: 217 AGDVDAQRVLALARKYFGKIP-ARRLPAGKPQNEPQQLGMRRVTVKAPAENPYVVMAWKT 275 Query: 272 PAIQR--GDPDFFALTVGNYVLGGGGFVSRLTDEVREKRGLSYSVYSGFSPLAQPGPFQI 329 PA+++ D D +AL V + VL G +RL+ + G + +V + +S +A+ GP Sbjct: 276 PALRQVEQDQDVYALDVLSAVLDGYDN-ARLSASLVRTGGKATAVGASYSGVAR-GPVLF 333 Query: 330 GLQTKKEQ---TAEALRVTRATLDKFMQEGPTAAELKAAKDNLAGGFALRIDSNAKLLEN 386 L+ + TA+ + R +++ +EG + EL+ K L + DS Sbjct: 334 TLEGSPAEGVTTAQLEGLLRGEVERIAREGVSEQELQRVKTQLIASQVYKRDSVFGQAME 393 Query: 387 LSVIGFYGLPLDYLDHWIERIRAVS 411 + ++ GL +D IER+++V+ Sbjct: 394 IGMMETAGLGQQNIDRIIERLKSVT 418 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.133 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,210,238 Number of extensions: 47292 Number of successful extensions: 218 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 215 Number of HSP's successfully gapped: 4 Length of query: 438 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 350 Effective length of database: 1,203,190 Effective search space: 421116500 Effective search space used: 421116500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)