BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0141.p (188 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [... 376 e-106 gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspiri... 137 7e-34 gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspiri... 105 3e-24 gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein ... 104 5e-24 gi|300309599|ref|YP_003773691.1| enoyl-CoA hydratase [Herbaspiri... 80 1e-16 >gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [Herbaspirillum seropedicae SmR1] Length = 262 Score = 376 bits (965), Expect = e-106, Method: Compositional matrix adjust. Identities = 187/188 (99%), Positives = 188/188 (100%) Query: 1 MIHKDAIATQLDVRAQYWRAIARFPKPILAAVNGYALGAGCELLMHADIAIAARGAKIGQ 60 MIHKDAIATQLDVRAQYWRAIARFPKPILAAVNGYALGAGCELLMHADIAIAARGAKIGQ Sbjct: 75 MIHKDAIATQLDVRAQYWRAIARFPKPILAAVNGYALGAGCELLMHADIAIAARGAKIGQ 134 Query: 61 PEINVGTIPGAGGTQRLIRTVGKPLAMKMVLSGEFISADQALQAGLVAEVVDDDATLERT 120 PEINVGT+PGAGGTQRLIRTVGKPLAMKMVLSGEFISADQALQAGLVAEVVDDDATLERT Sbjct: 135 PEINVGTLPGAGGTQRLIRTVGKPLAMKMVLSGEFISADQALQAGLVAEVVDDDATLERT 194 Query: 121 LALAHSIAQKSPLAVRLAKEAMLQSFELGLEAGLLFERKSFSLMAASADRQEGIAAFQEK 180 LALAHSIAQKSPLAVRLAKEAMLQSFELGLEAGLLFERKSFSLMAASADRQEGIAAFQEK Sbjct: 195 LALAHSIAQKSPLAVRLAKEAMLQSFELGLEAGLLFERKSFSLMAASADRQEGIAAFQEK 254 Query: 181 RAAVFSGR 188 RAAVFSGR Sbjct: 255 RAAVFSGR 262 >gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 258 Score = 137 bits (344), Expect = 7e-34, Method: Compositional matrix adjust. Identities = 77/168 (45%), Positives = 105/168 (62%) Query: 18 WRAIARFPKPILAAVNGYALGAGCELLMHADIAIAARGAKIGQPEINVGTIPGAGGTQRL 77 W + R KP++AAV GYALG GCEL M D IAA AK GQPEI +G +PGAGGTQRL Sbjct: 88 WETLRRIRKPVIAAVAGYALGGGCELAMMCDFIIAADNAKFGQPEIKLGIVPGAGGTQRL 147 Query: 78 IRTVGKPLAMKMVLSGEFISADQALQAGLVAEVVDDDATLERTLALAHSIAQKSPLAVRL 137 R V K AM + L+G + A++A +AGLV+ +V D LE +A A IA+ + Sbjct: 148 PRAVSKAKAMDLALTGRMMDAEEAERAGLVSRIVAADKLLEEAMAAAIIIAEMPRQVAMM 207 Query: 138 AKEAMLQSFELGLEAGLLFERKSFSLMAASADRQEGIAAFQEKRAAVF 185 K+++ +++E L G+ +ER F A+ D++EG+ AF EKR VF Sbjct: 208 VKDSVNRAYETTLSEGMKYERALFYSCFATEDQKEGMKAFLEKRQPVF 255 >gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 105 bits (262), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 64/160 (40%), Positives = 94/160 (58%) Query: 29 LAAVNGYALGAGCELLMHADIAIAARGAKIGQPEINVGTIPGAGGTQRLIRTVGKPLAMK 88 +AA+NGYA+G G E + DI IA A++ PE VG +P AGGTQ L VG+ A + Sbjct: 101 IAAINGYAMGGGLECALACDIRIAEEQAQMALPEAAVGLLPCAGGTQNLPWLVGEGWAKR 160 Query: 89 MVLSGEFISADQALQAGLVAEVVDDDATLERTLALAHSIAQKSPLAVRLAKEAMLQSFEL 148 M+L GE ++A+ AL+ GLV EVV ER LALA ++SP +V +K+ + + Sbjct: 161 MILCGERVNAETALRIGLVEEVVPRGQARERALALAQQAEKQSPSSVAASKQLIQGARHN 220 Query: 149 GLEAGLLFERKSFSLMAASADRQEGIAAFQEKRAAVFSGR 188 L L ER+ F + + D++EG+ AF EKR+ + + Sbjct: 221 PLGTILQTERELFVDLFDTQDQREGVQAFLEKRSPQWKNQ 260 >gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein [Herbaspirillum seropedicae SmR1] Length = 263 Score = 104 bits (259), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 66/170 (38%), Positives = 90/170 (52%) Query: 19 RAIARFPKPILAAVNGYALGAGCELLMHADIAIAARGAKIGQPEINVGTIPGAGGTQRLI 78 +++ P P++ AVNG A GAG L + DI +AAR A + +G IP GGT L Sbjct: 94 KSLRALPFPVICAVNGVAAGAGANLPLACDIVLAARSASFVEVFCKLGLIPDTGGTYFLP 153 Query: 79 RTVGKPLAMKMVLSGEFISADQALQAGLVAEVVDDDATLERTLALAHSIAQKSPLAVRLA 138 R VG AM M L GE ISA+QA + GL+ + VDDD +E A+A A+ + Sbjct: 154 RLVGTARAMGMALLGEKISAEQAERWGLIWKCVDDDQLMEEAHAMAAHFARAPTKGLAAT 213 Query: 139 KEAMLQSFELGLEAGLLFERKSFSLMAASADRQEGIAAFQEKRAAVFSGR 188 K + S L L ER + + S D +EG+ AF EKRA F+G+ Sbjct: 214 KATLYASPAHTLPEQLDLERDTMRALGRSRDYREGVTAFLEKRAPQFTGQ 263 >gi|300309599|ref|YP_003773691.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 79.7 bits (195), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 55/170 (32%), Positives = 82/170 (48%) Query: 16 QYWRAIARFPKPILAAVNGYALGAGCELLMHADIAIAARGAKIGQPEINVGTIPGAGGTQ 75 Q+ I+ KPI+AAV+G A+G G LL+H D+ AA AK P +G P A + Sbjct: 83 QFLWQISHASKPIVAAVSGVAVGIGTTLLLHCDLVYAADNAKFSMPFTQLGLCPEAASSL 142 Query: 76 RLIRTVGKPLAMKMVLSGEFISADQALQAGLVAEVVDDDATLERTLALAHSIAQKSPLAV 135 L G A + +L GE AD+A Q G V V+ + L A +A ++ Sbjct: 143 LLPHIAGYQRAAEKLLLGEAFGADEACQMGFVNRVMAQEELLPYAQQQAAKLAALPAASI 202 Query: 136 RLAKEAMLQSFELGLEAGLLFERKSFSLMAASADRQEGIAAFQEKRAAVF 185 R+ K+ M ++ E ++ E + FS M + + +E AF EKR F Sbjct: 203 RVTKQLMKKNRVTATEQQMVAEIEHFSRMLTAPEAKEAFTAFFEKRKPDF 252 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.134 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 533,776 Number of extensions: 21057 Number of successful extensions: 104 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 102 Number of HSP's successfully gapped: 8 Length of query: 188 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 108 Effective length of database: 1,241,070 Effective search space: 134035560 Effective search space used: 134035560 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 128 (53.9 bits)