BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0136.p (512 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313418|ref|YP_003777510.1| 3-hydroxyacyl-CoA dehydrogenase... 1050 0.0 gi|300309597|ref|YP_003773689.1| 3-hydroxyacyl-CoA dehydrogenase... 114 2e-26 gi|300313811|ref|YP_003777903.1| 3-hydroxyacyl-CoA dehydrogenase... 90 5e-19 >gi|300313418|ref|YP_003777510.1| 3-hydroxyacyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 512 Score = 1050 bits (2715), Expect = 0.0, Method: Compositional matrix adjust. Identities = 509/512 (99%), Positives = 510/512 (99%) Query: 1 MNNRKHPHALSRSALVAVIGSGTMGAGIAQVAAVAGHPVLLYDSREGAAGKAIGAICTNL 60 MNNRK+PHALSRSALVAVIGSGTMGAGIAQVAAVAGHPVLLYDSREGAAGKAIGAICTNL Sbjct: 1 MNNRKYPHALSRSALVAVIGSGTMGAGIAQVAAVAGHPVLLYDSREGAAGKAIGAICTNL 60 Query: 61 DKLVSKDKLTPALAQAASQRISVAHSLEELHQADLVIEAIVEQLAAKHELFGKLEAIVST 120 DKLVSKDKLTPALAQAASQRISVA SLEELHQADLVIEAIVEQLAAKHELFGKLEAIVST Sbjct: 61 DKLVSKDKLTPALAQAASQRISVAQSLEELHQADLVIEAIVEQLAAKHELFGKLEAIVST 120 Query: 121 RCILATNTSSLSITQIAAGLQHPERVVGMHFFNPVPMMALVEIISGLASDAALAQCLHDT 180 RCILATNTSSLSITQIAAGLQHPERVVGMHFFNPVPMMALVEIISGLASDAALAQCLHDT Sbjct: 121 RCILATNTSSLSITQIAAGLQHPERVVGMHFFNPVPMMALVEIISGLASDAALAQCLHDT 180 Query: 181 ALAWGKTPVMARSTPGFIVNRLARPYYAEGLRMLHEGGAQPATIDAVMREAGGFRMGPFE 240 ALAWGKTPVMARSTPGFIVNRLARPYYAEGLRMLHEGGAQPATIDAVMREAGGFRMGPFE Sbjct: 181 ALAWGKTPVMARSTPGFIVNRLARPYYAEGLRMLHEGGAQPATIDAVMREAGGFRMGPFE 240 Query: 241 LMDLIGHDVNFAVTQSVFHAYFNDPRFTPSLIQQELVHAGYLGRKSGRGFYRYGEAAGAP 300 LMDLIGHDVNFAVTQSVFHAYFNDPRFTPSLIQQELVHAGYLGRKSGRGFYRYGEAAGAP Sbjct: 241 LMDLIGHDVNFAVTQSVFHAYFNDPRFTPSLIQQELVHAGYLGRKSGRGFYRYGEAAGAP 300 Query: 301 RPETERDYPKPTATPQLYGDHPLVEMIHTRYPGQVQRHPARPDQLLMKVNAACLCITDGR 360 RPETERDYPKPTATPQLYGDHPLVEMIHTRYPGQVQRHPARPDQLLMKVNAACLCITDGR Sbjct: 301 RPETERDYPKPTATPQLYGDHPLVEMIHTRYPGQVQRHPARPDQLLMKVNAACLCITDGR 360 Query: 361 SATLRAWEHQEPDLVLVDLALDYADATRLALAKADQCRPQRYAEVAGVLQACGLDLSPMQ 420 SATLRAWEHQEPDLVLVDLALDYADATRLALAKADQCRPQRYAEVAGVLQACGLDLSPMQ Sbjct: 361 SATLRAWEHQEPDLVLVDLALDYADATRLALAKADQCRPQRYAEVAGVLQACGLDLSPMQ 420 Query: 421 DVPGMMVMRTVCMLVNEAADAVNQGVCNAADADMAMRKGVNYPRGPLAWANEIGIDTVTQ 480 DVPGMMVMRTVCMLVNEAADAVNQGVCNAADADMAMRKGVNYPRGPLAWANEIGIDTV Q Sbjct: 421 DVPGMMVMRTVCMLVNEAADAVNQGVCNAADADMAMRKGVNYPRGPLAWANEIGIDTVNQ 480 Query: 481 VLHHLCATYGEDRYRISPLLQRLYASGRKFHE 512 VLHHLCATYGEDRYRISPLLQRLYASGRKFHE Sbjct: 481 VLHHLCATYGEDRYRISPLLQRLYASGRKFHE 512 >gi|300309597|ref|YP_003773689.1| 3-hydroxyacyl-CoA dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 805 Score = 114 bits (284), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 97/290 (33%), Positives = 140/290 (48%), Gaps = 18/290 (6%) Query: 16 VAVIGSGTMGAGIAQVAAVAGHPVLLYD--SREGAAGKAIGAICTNLDKLVSK---DKLT 70 VAV+G+G MGA IA A PV+L+D ++EG + NL KL +K Sbjct: 19 VAVLGAGVMGAQIAAHCINARVPVVLFDLPAKEGPKNGIVQRAIENLKKLSPAPLGNKDD 78 Query: 71 PALAQAASQRISVAHSLEELHQADLVIEAIVEQLAAKHELFGKLEAIVSTRCILATNTSS 130 AL Q A+ L L DL+IEAI E++ KH+L+ K+ + + I A+NTS Sbjct: 79 AALIQVANYE----DDLAVLEGCDLIIEAIAERMDWKHDLYKKVAPHIGPKAIFASNTSG 134 Query: 131 LSITQIAAGLQH--PERVVGMHFFNPVPMMALVEIISGLASDA-ALAQCLHDTALAWGKT 187 LSI ++ G R G+HFFNP M LVE+I +++ L Q GK Sbjct: 135 LSINALSEGFDAGLKARFCGVHFFNPPRYMHLVELIPTQSTEPHILDQLEAFLTTTLGKG 194 Query: 188 PVMARSTPGFIVNRLARPYYAEGLRMLHEGGAQPATIDAVM-REAGGFRMGPFELMDLIG 246 V A TP F+ NR+ + + G +D + + G + G F D++G Sbjct: 195 VVRAFDTPNFVANRVGVFGILATIIEAEKYGLSVDVVDDLTGAKLGRAKSGTFRTADVVG 254 Query: 247 HDVNFAVTQSVFHAYFNDPRF----TPSLIQQELVHAGYLGRKSGRGFYR 292 D V +++ +DP F TP L+ + LV G LG+KSG GFY+ Sbjct: 255 LDTMGHVIRTMQDNLKDDPFFSSYATPPLLAK-LVEQGALGQKSGAGFYK 303 >gi|300313811|ref|YP_003777903.1| 3-hydroxyacyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 323 Score = 89.7 bits (221), Expect = 5e-19, Method: Compositional matrix adjust. Identities = 76/232 (32%), Positives = 111/232 (47%), Gaps = 11/232 (4%) Query: 14 ALVAVIGSGTMGAGIAQVAAVAGHPVLLYDSREGAAGKAIGAICTNLDKLVSKDKLTPAL 73 A VAV+G+G +GA A G V D A K AI + L + L Sbjct: 16 ARVAVVGTGVIGASWAAYFLAQGLEVSAVDPMPQAEQKLRAAIARHWPSLQAL-----GL 70 Query: 74 AQAASQ-RISVAHSLEE-LHQADLVIEAIVEQLAAKHELFGKLEAIVSTRCILATNTSSL 131 A+ ASQ R+ LE L AD V E E+ K +LF +++A + + +LA+++S L Sbjct: 71 AEHASQERLRFYDQLEAALEGADFVQENGPEREDLKIDLFRRMDAALPAQVVLASSSSGL 130 Query: 132 SITQIAAGLQHPERVVGMHFFNPVPMMALVEIISGLASDAALAQCLHDTALAWGKTPVMA 191 ++++ A +HP+RVV H FNP ++ LVE+I G + Q D A GK P+ Sbjct: 131 LMSKVQAACRHPQRVVLGHPFNPPHLIPLVEVIGGAQTSPEAVQTALDFYAAIGKKPIHV 190 Query: 192 RS-TPGFIVNRLARPYYAEGLRMLHEGGAQPATIDAVMREAGGFR---MGPF 239 R G I NRL + E ++ +G A ID + G R MGP+ Sbjct: 191 RKEVKGHIANRLQAALWREAFHLVEQGVASTEDIDTAIAYGPGLRWALMGPY 242 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,606,846 Number of extensions: 67716 Number of successful extensions: 264 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 256 Number of HSP's successfully gapped: 3 Length of query: 512 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 423 Effective length of database: 1,198,455 Effective search space: 506946465 Effective search space used: 506946465 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 133 (55.8 bits)