BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0135.p (263 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein ... 533 e-153 gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspiri... 144 5e-36 gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspiri... 130 8e-32 gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [... 130 1e-31 gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspiri... 128 5e-31 >gi|300313417|ref|YP_003777509.1| enoyl-CoA hydratase II protein [Herbaspirillum seropedicae SmR1] Length = 263 Score = 533 bits (1372), Expect = e-153, Method: Compositional matrix adjust. Identities = 262/263 (99%), Positives = 262/263 (99%) Query: 1 MSSPSILLENRDGIAVITLNRPDKLNSFTVAMHLELRDAIATLQADPSVRVLLLTGAGRG 60 MSSPSILLENRDGIAVITLNRPDKLNSFTVAMHLELRDAIATLQADPSVRVLLLTGAGRG Sbjct: 1 MSSPSILLENRDGIAVITLNRPDKLNSFTVAMHLELRDAIATLQADPSVRVLLLTGAGRG 60 Query: 61 FCAGQDLGDRAVKPGDDAVDLGESIDKYYGPLVKSLRALPFPVICAVNGVAAGAGANLPL 120 FCAGQDLGDRAVKPGDD VDLGESIDKYYGPLVKSLRALPFPVICAVNGVAAGAGANLPL Sbjct: 61 FCAGQDLGDRAVKPGDDGVDLGESIDKYYGPLVKSLRALPFPVICAVNGVAAGAGANLPL 120 Query: 121 ACDIVLAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAERWG 180 ACDIVLAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAERWG Sbjct: 121 ACDIVLAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAERWG 180 Query: 181 LIWKCVDDDQLMEEAHAMAAHFARAPTKGLAATKATLYASPAHTLPEQLDLERDTMRALG 240 LIWKCVDDDQLMEEAHAMAAHFARAPTKGLAATKATLYASPAHTLPEQLDLERDTMRALG Sbjct: 181 LIWKCVDDDQLMEEAHAMAAHFARAPTKGLAATKATLYASPAHTLPEQLDLERDTMRALG 240 Query: 241 RSRDYREGVTAFLEKRAPQFTGQ 263 RSRDYREGVTAFLEKRAPQFTGQ Sbjct: 241 RSRDYREGVTAFLEKRAPQFTGQ 263 >gi|300313175|ref|YP_003777267.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 258 Score = 144 bits (364), Expect = 5e-36, Method: Compositional matrix adjust. Identities = 96/262 (36%), Positives = 145/262 (55%), Gaps = 9/262 (3%) Query: 1 MSSPSILLENRDGIAVITLNRPDKLNSFTVAMHLELRDAIATLQADPSVRVLLLTGAGRG 60 MS +I +E + + +I L+RP LN+ + + EL +A+ A + +++TG+ + Sbjct: 1 MSYENIQVETHEKVGLIRLHRPKALNALSDGLMTELGEALLAFDAQEEIGCIIITGSEKA 60 Query: 61 FCAGQDLGDRAVKPGDDAVDL--GESIDKYYGPLVKSLRALPFPVICAVNGVAAGAGANL 118 F AG D+ A G D +D+ GE I + + ++LR + PVI AV G A G G L Sbjct: 61 FAAGADISAMA---GYDYMDVFKGEFITRNW----ETLRRIRKPVIAAVAGYALGGGCEL 113 Query: 119 PLACDIVLAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAER 178 + CD ++AA +A F + KLG++P GGT LPR V A+AM +AL G + AE+AER Sbjct: 114 AMMCDFIIAADNAKFGQPEIKLGIVPGAGGTQRLPRAVSKAKAMDLALTGRMMDAEEAER 173 Query: 179 WGLIWKCVDDDQLMEEAHAMAAHFARAPTKGLAATKATLYASPAHTLPEQLDLERDTMRA 238 GL+ + V D+L+EEA A A A P + K ++ + TL E + ER + Sbjct: 174 AGLVSRIVAADKLLEEAMAAAIIIAEMPRQVAMMVKDSVNRAYETTLSEGMKYERALFYS 233 Query: 239 LGRSRDYREGVTAFLEKRAPQF 260 + D +EG+ AFLEKR P F Sbjct: 234 CFATEDQKEGMKAFLEKRQPVF 255 >gi|300313068|ref|YP_003777160.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 263 Score = 130 bits (328), Expect = 8e-32, Method: Compositional matrix adjust. Identities = 86/258 (33%), Positives = 124/258 (48%), Gaps = 5/258 (1%) Query: 4 PSIL-LENRDGIAVITLNRPDKLNSFTVAMHLELRDAIATLQADPSVRVLLLTGAGRGFC 62 P IL E++ GI +TLNRP N+ + M L+ + +++D +VR ++L G+ FC Sbjct: 6 PDILEREDQQGITTLTLNRPQHFNALSEEMLTALQAQLDDIKSDSTVRCVILAANGKAFC 65 Query: 63 AGQDLGDRAVKPGDDAVDLGESIDKYYGPLVKSLRALPFPVICAVNGVAAGAGANLPLAC 122 AG DL P E++ L++SLR LP PVI V G+A AG L C Sbjct: 66 AGHDLRQMQATPQQT---YYEALFHRCSHLMQSLRELPVPVIAKVQGLATAAGCQLVATC 122 Query: 123 DIVLAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAERWGLI 182 D+ +AA SA F +GL T L R + RA M + G+ I A A WGLI Sbjct: 123 DLAIAAESARFAVSGINVGLFCSTPAVA-LTRNLPIKRAFEMLVTGKFIDAATAAAWGLI 181 Query: 183 WKCVDDDQLMEEAHAMAAHFARAPTKGLAATKATLYASPAHTLPEQLDLERDTMRALGRS 242 + V + +L A+A P +A KA +Y LP+ + M Sbjct: 182 NQAVPEQELDSATQALAREICSKPAISIARGKAMVYRQQMMALPDAYAHAAEVMACNIMD 241 Query: 243 RDYREGVTAFLEKRAPQF 260 + +EG+TAFL+KR P + Sbjct: 242 PEAQEGITAFLDKRKPNW 259 >gi|300313422|ref|YP_003777514.1| enoyl-CoA hydratase/isomerase [Herbaspirillum seropedicae SmR1] Length = 262 Score = 130 bits (326), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 94/259 (36%), Positives = 132/259 (50%), Gaps = 8/259 (3%) Query: 7 LLENRDGIAVI--TLNRPDKLNSFTVAMHLELRDAIATLQADPSVRVLLLTGAGRGFCAG 64 LL R G V+ TLNRP N+ E+ A+A + D SVRV++++G + F AG Sbjct: 10 LLAARHGEHVLHLTLNRPALRNALRNQSLREIVAALARAEEDDSVRVVVISGNEKAFAAG 69 Query: 65 QDLGDRAVKPGDDAVDLGESIDKYYGPLVKSLRALPFPVICAVNGVAAGAGANLPLACDI 124 DL + K DA+ + Y +++ P P++ AVNG A GAG L + DI Sbjct: 70 ADLNEMIHK---DAIATQLDVRAQYW---RAIARFPKPILAAVNGYALGAGCELLMHADI 123 Query: 125 VLAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAERWGLIWK 184 +AAR A + +G +P GGT L R VG AM M L GE ISA+QA + GL+ + Sbjct: 124 AIAARGAKIGQPEINVGTLPGAGGTQRLIRTVGKPLAMKMVLSGEFISADQALQAGLVAE 183 Query: 185 CVDDDQLMEEAHAMAAHFARAPTKGLAATKATLYASPAHTLPEQLDLERDTMRALGRSRD 244 VDDD +E A+A A+ + K + S L L ER + + S D Sbjct: 184 VVDDDATLERTLALAHSIAQKSPLAVRLAKEAMLQSFELGLEAGLLFERKSFSLMAASAD 243 Query: 245 YREGVTAFLEKRAPQFTGQ 263 +EG+ AF EKRA F+G+ Sbjct: 244 RQEGIAAFQEKRAAVFSGR 262 >gi|300311864|ref|YP_003775956.1| enoyl-CoA hydratase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 128 bits (322), Expect = 5e-31, Method: Compositional matrix adjust. Identities = 93/260 (35%), Positives = 133/260 (51%), Gaps = 6/260 (2%) Query: 5 SILLENRDGIAVITLNRPDKLNSFTVAMHLELRDAIATLQADPSVRVLLLTGAG-RGFCA 63 ++ LE AV+TL+ P N++T A +L + L AD + L++TG G + F A Sbjct: 6 NLKLEKTGHTAVLTLSNPPA-NTWTRAALADLTRLVKELNADRDIYTLVVTGEGEKFFSA 64 Query: 64 GQDLGDRAVKPGDDAVDLGESIDKYYGPLVKSLRALPFPVICAVNGVAAGAGANLPLACD 123 G DL + GD A + + + +G ++L A I A+NG A G G LACD Sbjct: 65 GADL--KLFADGDKA--MAREMARRFGEAFETLSAFRGVSIAAINGYAMGGGLECALACD 120 Query: 124 IVLAARSASFVEVFCKLGLIPDTGGTYFLPRLVGTARAMGMALLGEKISAEQAERWGLIW 183 I +A A +GL+P GGT LP LVG A M L GE+++AE A R GL+ Sbjct: 121 IRIAEEQAQMALPEAAVGLLPCAGGTQNLPWLVGEGWAKRMILCGERVNAETALRIGLVE 180 Query: 184 KCVDDDQLMEEAHAMAAHFARAPTKGLAATKATLYASPAHTLPEQLDLERDTMRALGRSR 243 + V Q E A A+A + +AA+K + + + L L ER+ L ++ Sbjct: 181 EVVPRGQARERALALAQQAEKQSPSSVAASKQLIQGARHNPLGTILQTERELFVDLFDTQ 240 Query: 244 DYREGVTAFLEKRAPQFTGQ 263 D REGV AFLEKR+PQ+ Q Sbjct: 241 DQREGVQAFLEKRSPQWKNQ 260 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 789,161 Number of extensions: 30825 Number of successful extensions: 115 Number of sequences better than 1.0e-08: 10 Number of HSP's gapped: 105 Number of HSP's successfully gapped: 10 Length of query: 263 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 180 Effective length of database: 1,226,865 Effective search space: 220835700 Effective search space used: 220835700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)