BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0134.p (561 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313416|ref|YP_003777508.1| NAD-dependent aldehyde dehydrog... 1122 0.0 gi|300313866|ref|YP_003777958.1| benzaldehyde dehydrogenase [Her... 65 2e-11 gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrog... 60 4e-10 >gi|300313416|ref|YP_003777508.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 561 Score = 1122 bits (2903), Expect = 0.0, Method: Compositional matrix adjust. Identities = 554/561 (98%), Positives = 555/561 (98%) Query: 1 MPTDFFNRHRATLEQAVSALQQRGYWSPYNESPSPRFYGEGAAEAGRAAYEARLERPFLF 60 MPTDFFNRHRATLEQAVSALQQRGYWSPYNESPSPRFYGEGAAEAGRAAYEARLERPFLF Sbjct: 1 MPTDFFNRHRATLEQAVSALQQRGYWSPYNESPSPRFYGEGAAEAGRAAYEARLERPFLF 60 Query: 61 TPEQAGGDRWVGAEVSPYGPPLGIRYPAPDLDRLLPSMQLAAASWREAGPQVWAGVALEI 120 TPEQAGGDRWVGAEVSPYGPPLGIRYPAPDLDRLLPSMQ AAASWREAGPQVWAGVALEI Sbjct: 61 TPEQAGGDRWVGAEVSPYGPPLGIRYPAPDLDRLLPSMQRAAASWREAGPQVWAGVALEI 120 Query: 121 VQRLNARSFEIAHAVQHTTGQAFMMAFQAGGPHAQDRALEAIACAWQQLQLIPAEARWEK 180 VQRLNARSFEIAHAVQHTTGQAF MAFQAGGPHAQDRALEAIACAWQQLQLIPAEARWEK Sbjct: 121 VQRLNARSFEIAHAVQHTTGQAFTMAFQAGGPHAQDRALEAIACAWQQLQLIPAEARWEK 180 Query: 181 PQGKHAPLVMRKTWRIVPRGIGLVIGCCTFPTWNSYPGLFANLATGNAVIVKPHPGAILP 240 PQGKHAPLVMRKTWRIVPRGIGLVIGCCTFPTWNSYPGLFANLATGNAVIVKPHPGAILP Sbjct: 181 PQGKHAPLVMRKTWRIVPRGIGLVIGCCTFPTWNSYPGLFANLATGNAVIVKPHPGAILP 240 Query: 241 LAITVQVAREVLAEAGFALDVVTLAAHDASDTSLARTLALRPEIGLIDFTGSSANGDWLE 300 LAITVQVAREVLAEAGFA DVVTLAAHDASDT LARTLALR EIGLIDFTGSSANGDWLE Sbjct: 241 LAITVQVAREVLAEAGFAPDVVTLAAHDASDTGLARTLALRHEIGLIDFTGSSANGDWLE 300 Query: 301 DRARQARVLTEKSGINQIIIDSTDNLAGMVQNIALSLALYSGQMCTTPQNIYIPRDGVRT 360 +RARQARVLTEKSGINQIIIDSTDNLAGMVQNIALSLALYSGQMCTTPQNIYIPRDGVRT Sbjct: 301 ERARQARVLTEKSGINQIIIDSTDNLAGMVQNIALSLALYSGQMCTTPQNIYIPRDGVRT 360 Query: 361 PQGQLSFVEVSQAIVQAVAHLTTDSAKAVELLGAIQNEAVLQRIELARGKGQVLLESTAL 420 PQGQLSFVEVSQAIVQAVAHLTTDSAKAVELLGAIQNEAVLQRIELARGKGQVLLESTAL Sbjct: 361 PQGQLSFVEVSQAIVQAVAHLTTDSAKAVELLGAIQNEAVLQRIELARGKGQVLLESTAL 420 Query: 421 VHPQFPGARVRTPLVMLARRNEAITEEYFGPISFIVPVDDTATAIALAQRIATDQGALTL 480 VHPQFPGARVRTPLVMLARRNEAITEEYFGPISFIVPVDDTATAIALAQRIATDQGALTL Sbjct: 421 VHPQFPGARVRTPLVMLARRNEAITEEYFGPISFIVPVDDTATAIALAQRIATDQGALTL 480 Query: 481 SAYTTDATLRQGLQHAAADAGVALSLNLTGGVLVNQSAAFSDYHGTGANPAANCTLTDAD 540 SAYTTDATLRQGLQHAAADAGVALSLNLTGGVLVNQSAAFSDYHGTGANPAANCTLTDAD Sbjct: 481 SAYTTDATLRQGLQHAAADAGVALSLNLTGGVLVNQSAAFSDYHGTGANPAANCTLTDAD 540 Query: 541 FVAARYRVVQTREPAASTTTA 561 FVAARYRVVQTREP ASTTTA Sbjct: 541 FVAARYRVVQTREPVASTTTA 561 >gi|300313866|ref|YP_003777958.1| benzaldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 487 Score = 65.1 bits (157), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 88/323 (27%), Positives = 129/323 (39%), Gaps = 51/323 (15%) Query: 195 RIVPRGIGLVIGCCTFPTWNSYPGLFANLATGNAVIVKPHPGAILPLAITVQVAREVLAE 254 R P G+ VI FP + + LA GNAV+VKP P++ +AR + + Sbjct: 142 RRAPLGVIGVISPFNFPLILTLRTVAPALAAGNAVVVKPD--LRTPVSGGFMLAR-IFEQ 198 Query: 255 AGFALDVVTLAAHDASDTSLARTLALRPEIGLIDFTGSSANGDWLEDRA-RQARVLT-EK 312 AG ++ + A L P + +I FTGS A G + + A R + L+ E Sbjct: 199 AGLPAGLLHVLPGGAET---GEALVTAPGVPMIAFTGSPAVGRRIGELAGRHLKKLSLEL 255 Query: 313 SGINQIIIDSTDNLAGMVQNIALSLALYSGQMCTTPQNIYIPRDGVRTPQGQLSFVE-VS 371 G N ++I +L + A L+ GQ+C I + S E + Sbjct: 256 GGANALVILEDADLDVAASHAAWGAWLHQGQICMAANRILV----------HASLEEGLL 305 Query: 372 QAIVQAVAHL-TTDSAKAVELLGAIQNEAVLQRIE-------------LARGKGQVLLES 417 Q + HL D A LG + +E LQR+ +A G+ + L Sbjct: 306 QRLADKARHLPVGDGASGQVALGPMIDERQLQRVHALVQESVAAGAQLVAGGRYEQLFYQ 365 Query: 418 TALVHPQFPGARVRTPLVMLARRNEAITEEYFGPISFIVPVDDTATAIALAQRIATDQGA 477 ++ PG RV EE FGP++ ++ D+ AIALA R A A Sbjct: 366 PTVLGGVRPGMRV-------------FEEEIFGPVASVIRFDNDEDAIALANRHAGGLAA 412 Query: 478 LTLS-----AYTTDATLRQGLQH 495 LS A A LRQG+ H Sbjct: 413 GVLSPSVGRAMAVAARLRQGMVH 435 >gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 60.1 bits (144), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 80/287 (27%), Positives = 123/287 (42%), Gaps = 29/287 (10%) Query: 213 WNSYPGLFAN-----LATGNAVIVKPHPGAILPLAITVQVAREVLAEAGF---ALDVVTL 264 WN +F LA GN +VKP A L + ++VA E+ AE GF AL++VT Sbjct: 147 WNYPMQIFGRCVGGALAAGNTCVVKPAEDACLSI---LRVA-ELAAEVGFPEGALNIVTG 202 Query: 265 AAHDASDTSLARTLALRPEIGLIDFTGSSANGDWLEDRARQAR--VLTEKSGINQIIIDS 322 H+A D L P++ I FTGS G + A + V E G + I+ Sbjct: 203 YGHEAGDL-----LVKHPDVNHISFTGSPNVGKLVVRAAAEHHTPVTLELGGKSPQIVFE 257 Query: 323 TDNLAGMVQNIALSLALYSGQMCTTPQNIYIPRDGVRTPQGQLSFVEVSQAIVQAVAHLT 382 ++ M+ + ++ +GQ C+ + + R +LS + SQ V + + + Sbjct: 258 DADMEAMLPVVVNAIVQNAGQTCSAGSRLLVQRGAYEQVLERLSGL-FSQLQVGS-SQMD 315 Query: 383 TDSAKAVELLGAIQNEAVLQRIELARGKGQVLLESTALVHPQFPGARVRTPLVM--LARR 440 D L+ Q E V + A+ G L + G + P ++ + R Sbjct: 316 LDCGP---LIRKTQQERVAGFLSDAQRDGIALAAQGKIAAGVPTGGFYQAPALLRDVPPR 372 Query: 441 NEAITEEYFGPISFIVPVDDTATAIALAQRIATDQGALTLSAYTTDA 487 + EE FGP+ +P +D A AIALA TD G L S +T D Sbjct: 373 HRLAQEEVFGPVLAAMPFEDEAHAIALAN--GTDYG-LVASVWTRDG 416 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.132 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,829,817 Number of extensions: 79622 Number of successful extensions: 342 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 343 Number of HSP's successfully gapped: 6 Length of query: 561 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 471 Effective length of database: 1,193,720 Effective search space: 562242120 Effective search space used: 562242120 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)