BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0133.p (356 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313415|ref|YP_003777507.1| phenylacetic acid degradation N... 736 0.0 gi|300311823|ref|YP_003775915.1| nitric oxide dioxygenase [Herba... 91 1e-19 gi|300313278|ref|YP_003777370.1| vanillate O-demethylase (vanill... 76 4e-15 gi|300312623|ref|YP_003776715.1| chaperone activity ATPase [Herb... 58 1e-09 >gi|300313415|ref|YP_003777507.1| phenylacetic acid degradation NADH oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 356 Score = 736 bits (1899), Expect = 0.0, Method: Compositional matrix adjust. Identities = 352/356 (98%), Positives = 353/356 (99%) Query: 1 MSKFYPLTISDVKQETRDTIVVSFAVPAELQDTFSYQQGQHLTLRSEINGEDLRRSYSIC 60 MSKFYPLTISDVKQETRDTIVVSFAVPAELQDTFSYQQGQHLTLRSEINGEDLRRSYSIC Sbjct: 1 MSKFYPLTISDVKQETRDTIVVSFAVPAELQDTFSYQQGQHLTLRSEINGEDLRRSYSIC 60 Query: 61 SAVQERQLRVAIKRAPGGLFSNWANESFVPGQRIDVMPPMGHFNVPLEAGNRKHYLAFAA 120 SAVQERQLRVAIKRAPGGLFSNWANESFVPGQRIDVMPPMGHFNVPLEAGNRKHYLAFAA Sbjct: 61 SAVQERQLRVAIKRAPGGLFSNWANESFVPGQRIDVMPPMGHFNVPLEAGNRKHYLAFAA 120 Query: 121 GSGITPMMSIIKTTLLSEPHSHFTLVYANRASSSVIFKEELTDLKDTYLERFNVVYVMSR 180 GSGITPMMSIIKTTLLSEPHSHFTLVYANRASSSVIFKEELTDLKD YLERFNVVYVMSR Sbjct: 121 GSGITPMMSIIKTTLLSEPHSHFTLVYANRASSSVIFKEELTDLKDAYLERFNVVYVMSR 180 Query: 181 EQQDVELFNGRIDRAKCDAFFASWIDLKDVDAAFLCGPEDMVQAVAASLQAHGLPKTQIK 240 EQQDVELFNGRIDRAKCDAFFASWIDLKDVDAAFLCGPE+MVQAVAASLQAHGLPKTQIK Sbjct: 181 EQQDVELFNGRIDRAKCDAFFASWIDLKDVDAAFLCGPEEMVQAVAASLQAHGLPKTQIK 240 Query: 241 TELFAASTPPRAHAARAVVGKQECEVTVIVDGYHNVFTMDKEKESVLEAGLKHGIDLRYS 300 TELFAASTPPRAHAAR VVGKQECEVTVIVDGYHNVFTMDKEKESVLEAGLKHGIDLRYS Sbjct: 241 TELFAASTPPRAHAARTVVGKQECEVTVIVDGYHNVFTMDKEKESVLEAGLKHGIDLRYS 300 Query: 301 CKGGVCATCRGKVVEGKVDMDANYALEDYEIARGFVLSCQSFPVSDKLLLDFDQDN 356 CKGGVCATCR KVVEGKVDMDANYALEDYEIARGFVLSCQSFPVSDKLLLDFDQDN Sbjct: 301 CKGGVCATCRCKVVEGKVDMDANYALEDYEIARGFVLSCQSFPVSDKLLLDFDQDN 356 >gi|300311823|ref|YP_003775915.1| nitric oxide dioxygenase [Herbaspirillum seropedicae SmR1] Length = 397 Score = 90.9 bits (224), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 62/228 (27%), Positives = 105/228 (46%), Gaps = 14/228 (6%) Query: 26 VPAELQDTFSYQQGQHLTLRSEINGEDLRRSYSICS----AVQERQLRVAIKRAPGGLFS 81 VP + + + GQ++ LR ++GE+ RR YS+ + A Q Q R+++KR PGG S Sbjct: 176 VPQDGGELLDFAPGQYIGLRMVVDGEEQRRQYSLSALLRQAGQPPQYRISVKREPGGKVS 235 Query: 82 NWANESFVPGQRIDVMPPMGHFNVPLEAGNRKHYLAFAAGSGITPMMSIIKTTLLSEPHS 141 ++ G +++ PP G F + + K + G GITPMMS++ L S Sbjct: 236 RQLHDQIHVGSVVELFPPAGEFTL---MASDKPLALISGGVGITPMMSMLAAALPSGRPI 292 Query: 142 HFTLVYANRASSSVIFKEELTDLKDTYLE-RFNVVYVMSREQQDVELFNGRIDRAKCDAF 200 HF ++A R F+ +L +L + + + Y R+ G I+R+ + Sbjct: 293 HF--IHAARHGGVHAFRRQLEELAARHPQLQLRFCYEQPRQGDAAPHATGLIERSLLQQW 350 Query: 201 FASWIDLKDVDAAFLCGPEDMVQAVAASLQAHGLPKTQIKTELFAAST 248 DL A+ GP+ ++A+ L G+P Q + E F + Sbjct: 351 LPGNPDLD----AYFVGPKSFMKAIKRQLAELGVPAAQSRYEFFGPAA 394 >gi|300313278|ref|YP_003777370.1| vanillate O-demethylase (vanillate degradation ferredoxin-like) oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 318 Score = 76.3 bits (186), Expect = 4e-15, Method: Compositional matrix adjust. Identities = 78/305 (25%), Positives = 134/305 (43%), Gaps = 39/305 (12%) Query: 53 LRRSYSICSAVQER-QLRVAIKR---APGGLFSNWANESFVPGQRIDVMPPMGHFN-VPL 107 L R YS+C+A E+ + ++ + R + GG S +E+ G + + P F VP Sbjct: 48 LVRQYSLCNAPHEQHRYQIGVLRDVNSRGG--SVAVHEALKLGDILTISAPRNQFALVPA 105 Query: 108 EAGNRKHYLAFAAGSGITPMMSIIKTTLLSEPHSHFTLVYANRASSSVIFKEELTDLKDT 167 + H L A G G+TP++ + + + + F + Y R+ + F+E + Sbjct: 106 Q-----HSLLLAGGIGVTPILCMAEALAAAG--ASFEMHYCARSPARQAFRERIA--ASG 156 Query: 168 YLERFNVVYVMSREQQDVELFNGRIDRAKCDAFFASWIDLKDVDAAFLCGPEDMVQAVAA 227 + R Y Q ++L DA A + ++CGP + V Sbjct: 157 FAARVVHHYDDGEAAQKLDL----------DALLAQADPATHL---YVCGPAGFIAHVVD 203 Query: 228 SLQAHGLPKTQIKTELFAASTPPRAHAARAVVGKQECEVTVIVDGYHNVFTMDKEKESVL 287 + +A G P+ Q+ E F A+ P +A G EV + G V+T+ E+ +V+ Sbjct: 204 TARARGWPEAQVHFEYFGAA-PVKAD------GNGAFEVKLASSG--QVYTIPPER-TVI 253 Query: 288 EAGLKHGIDLRYSCKGGVCATCRGKVVEGKVDMDANYALEDYEIARGFVLSCQSFPVSDK 347 + +HG+D+ SC+ G+C TC +V+EG+ D Y ++ A C S S Sbjct: 254 QVLCEHGVDVPVSCEQGICGTCLTRVLEGEPDHRDQYLTDEERAANDQFTPCCSRARSAL 313 Query: 348 LLLDF 352 L+LD Sbjct: 314 LVLDL 318 >gi|300312623|ref|YP_003776715.1| chaperone activity ATPase [Herbaspirillum seropedicae SmR1] Length = 349 Score = 58.2 bits (139), Expect = 1e-09, Method: Compositional matrix adjust. Identities = 58/246 (23%), Positives = 112/246 (45%), Gaps = 19/246 (7%) Query: 6 PLTISDVKQETRDTIVVSFAVPAELQDTFSYQQGQHLTLRSEINGEDLRRSYSICSAV-Q 64 P+ ++ + + D IV+S +PA D Y+ GQ++ + + RRSYS+ +A + Sbjct: 104 PVRVAKLDKVADDVIVLSLQLPAN--DRLQYKAGQYVEF---LLRDGKRRSYSMATAPHK 158 Query: 65 ERQLRVAIKRAPGGLFSNWANESFVPGQRIDVMPPMGHFNVPLEAGNRKHYLAFAAGSGI 124 + + + I+ PGGLF++ + + + P+G F L + K + A+G+G Sbjct: 159 DEHMSLHIRHLPGGLFTDQVFTTLKERDILRIEGPLGTFF--LREDSDKPIILLASGTGF 216 Query: 125 TPMMSIIKTTLLSEPHSHFTLVYANRASSSVIFKEELTDLKDTYLERFNVVYVMSREQQD 184 P+ +I++ + TL + R ++ + L + L F V V+S Q + Sbjct: 217 APIKAIVEQLEHAGSKRPVTLYWGGRRPQD-LYMDALCQQWEQTLPNFKYVPVVSNAQAE 275 Query: 185 VELFNGR---IDRAKCDAFFASWIDLKDVDAAFLCGPEDMVQAVAASLQAH-GLPKTQIK 240 + ++GR + RA + F DL + CG +V++ A GLP+ + Sbjct: 276 -DNWSGRTGFVHRAVMEDF----PDLSG-HQVYACGAPIVVESAQRDFSAQCGLPQDEFY 329 Query: 241 TELFAA 246 + F + Sbjct: 330 ADSFTS 335 Score = 56.6 bits (135), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 33/84 (39%), Positives = 48/84 (57%), Gaps = 5/84 (5%) Query: 265 EVTVIVDGYHNVFTMDKEKESVLEAGLKHGIDLRYSCKGGVCATCRGKVVEGKVDMDANY 324 +VTV G FT D + ES+L A ++ G+ L Y CK G C++C+GK+V G+V+ + Sbjct: 4 QVTVQPSGRQ--FTCDAD-ESILTAAIRAGVGLPYGCKNGACSSCKGKLVSGQVEHGPHQ 60 Query: 325 --ALEDYEIARGFVLSCQSFPVSD 346 AL +E G L C + P SD Sbjct: 61 ERALSAHEQQLGMALFCVAKPQSD 84 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,072,371 Number of extensions: 42470 Number of successful extensions: 105 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 104 Number of HSP's successfully gapped: 6 Length of query: 356 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 270 Effective length of database: 1,212,660 Effective search space: 327418200 Effective search space used: 327418200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)