BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0126.p (381 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313408|ref|YP_003777500.1| branched-chain amino acid ABC t... 772 0.0 gi|300310681|ref|YP_003774773.1| amino acid ABC transporter peri... 341 5e-95 gi|300313298|ref|YP_003777390.1| branched-chain amino acid ABC t... 328 3e-91 gi|300312236|ref|YP_003776328.1| amino acid ABC transporter peri... 70 4e-13 gi|300311113|ref|YP_003775205.1| branched-chain amino acid ABC t... 67 2e-12 >gi|300313408|ref|YP_003777500.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 381 Score = 772 bits (1994), Expect = 0.0, Method: Compositional matrix adjust. Identities = 380/381 (99%), Positives = 380/381 (99%) Query: 1 MQFARPLLALALAGCALVARADINVGVTVSTTGPAASLGIPEKNTIALLPTTIAGHKVNY 60 MQFARPLLALALAGCALVARADINVGVTVSTTGPAASLGIPEKNTIALLPTTIAGHKVNY Sbjct: 1 MQFARPLLALALAGCALVARADINVGVTVSTTGPAASLGIPEKNTIALLPTTIAGHKVNY 60 Query: 61 IILDDASDTTQAVKNARRFVSENNVDVIVGSSVSPNSISMIEVAADAQTPMISLAAAARI 120 IILDDASDTTQAVKNARRFVSENNVDVIVGSSVSPNSISMIEVAADAQTPMISLAAAARI Sbjct: 61 IILDDASDTTQAVKNARRFVSENNVDVIVGSSVSPNSISMIEVAADAQTPMISLAAAARI 120 Query: 121 VEPMDAKKKWVFKTPQNDIMMSLAIARDMVARGYQRIAFIGYSNAYGEGWYEEFKKAAGL 180 VEPMDAKKKWVFKTPQNDIMMSLAIARDMVARGYQRIAFIGYSNAYGEGWYEEFKKAAGL Sbjct: 121 VEPMDAKKKWVFKTPQNDIMMSLAIARDMVARGYQRIAFIGYSNAYGEGWYEEFKKAAGL 180 Query: 181 KKLELVANERFAPTDSSTTAQALKLVAAKPDAILIAASGTPAVVPQRALKERGFKGQIYQ 240 KKLELVANERFAPTDSSTTAQALKLVAAKPDAILIAASGTPAVVPQRALKERGFKGQIYQ Sbjct: 181 KKLELVANERFAPTDSSTTAQALKLVAAKPDAILIAASGTPAVVPQRALKERGFKGQIYQ 240 Query: 241 THGVANADFLRVGGKMLDGTLLPAGPVLVAAQLPASNPIRKVALDYTTKYEAVHGKDSVS 300 THGVANADFLRVGGKMLDGTLLPAGPVLVAAQLPASNPIRKVALDYTTKYEAVHGKDSVS Sbjct: 241 THGVANADFLRVGGKMLDGTLLPAGPVLVAAQLPASNPIRKVALDYTTKYEAVHGKDSVS 300 Query: 301 LFGGYAWDAGVLLRWAIPVALKGAKPGTPEFRAALRDALEATKEVTGVHGVYTMSSTDHL 360 LFGGYAWDAGVLLRWAIPVALKGAKPGTPEFRAALRD LEATKEVTGVHGVYTMSSTDHL Sbjct: 301 LFGGYAWDAGVLLRWAIPVALKGAKPGTPEFRAALRDVLEATKEVTGVHGVYTMSSTDHL 360 Query: 361 GLDQRAAVMVKIVDGAWQYQP 381 GLDQRAAVMVKIVDGAWQYQP Sbjct: 361 GLDQRAAVMVKIVDGAWQYQP 381 >gi|300310681|ref|YP_003774773.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 385 Score = 341 bits (875), Expect = 5e-95, Method: Compositional matrix adjust. Identities = 176/381 (46%), Positives = 246/381 (64%), Gaps = 5/381 (1%) Query: 1 MQFARPLLALALAGCALVAR----ADINVGVTVSTTGPAASLGIPEKNTIALLPTTIAGH 56 M R + A+ L G + +A A + VGV S+TGP + +GIP+KNT+ LLPT I Sbjct: 1 MLHKRLIAAVVLLGMSSLAMHPALAQVKVGVVTSSTGPVSLVGIPQKNTVPLLPTKIGDL 60 Query: 57 KVNYIILDDASDTTQAVKNARRFVSENNVDVIVGSSVSPNSISMIEVAADAQTPMISLAA 116 KV YI LDDASD T++V ++ ++E+NVD I+G S SPN++ +I A+A P+++ Sbjct: 61 KVEYISLDDASDPTKSVTEIKKLIAEHNVDAIIGPSGSPNAMGVISFVAEAGVPLLAPVG 120 Query: 117 AARIVEPMDAKKKWVFKTPQNDIMMSLAIARDMVARGYQRIAFIGYSNAYGEGWYEEFKK 176 A +V PM +KKWVFKT QND +++ A+ MV + FI ++ +GE W F Sbjct: 121 TAAVVLPMTEQKKWVFKTTQNDDLIAKALVDQMVKTKVKTAGFIAVNDPFGENWARVFSD 180 Query: 177 AAGLKKLELVANERFAPTDSSTTAQALKLVAAKPDAILIAASGTPAVVPQRALKERGFKG 236 A +++VANER+ TD+S TAQ+LK+VAA PDA+L+AA G V+PQ L ++G+KG Sbjct: 181 LAAKNNIKIVANERYQRTDTSVTAQSLKIVAANPDAVLVAAPGAATVLPQTTLFDQGYKG 240 Query: 237 QIYQTHGVANADFLRVGGKMLDGTLLPAGPVLVAAQLPASNPIRKVALDYTTKYEAVHGK 296 +IYQTHG A FL++G ++GT+L A +LV ++P SNP +KVA DY Y+ ++ Sbjct: 241 KIYQTHGAALPAFLQLGKNKVEGTILAASLMLVLPEIPDSNPSKKVATDYIEAYKKMYNA 300 Query: 297 DSVSLFGGYAWDAGVLLRWAIPVALKGAKPGTPEFRAALRDALEATKEVTGVHGVYTMSS 356 + FG +DAG+LL AIPVA + AKPG+ EFRAALRDALE TKEV G GVY MS+ Sbjct: 301 VPAT-FGANVYDAGLLLEKAIPVAARKAKPGSKEFRAALRDALENTKEVVGTQGVYNMSA 359 Query: 357 TDHLGLDQRAAVMVKIVDGAW 377 TDH G D R ++ + G W Sbjct: 360 TDHSGFDDRGRELITVKGGNW 380 >gi|300313298|ref|YP_003777390.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 381 Score = 328 bits (842), Expect = 3e-91, Method: Compositional matrix adjust. Identities = 160/368 (43%), Positives = 242/368 (65%), Gaps = 4/368 (1%) Query: 11 ALAGCALVARADINVGVTVSTTGPAASLGIPEKNTIALLPTTIAGHKVNYIILDDASDTT 70 A+ CA +A A++ +GV +S TGP + LGIP KN AL P TIAG KV IILDDA+D T Sbjct: 14 AMLACAGIAHAELTIGVVMSLTGPGSGLGIPAKNGFALWPETIAGEKVKMIILDDATDPT 73 Query: 71 QAVKNARRFVSENNVDVIVGSSVSPNSISMIEVAADAQTPMISLAAAARIVEPMDAKKKW 130 QA KNARR V+E+ VD+I+GS+ P ++++ +VA + QT ++++ +E + K W Sbjct: 74 QASKNARRLVAEDKVDLIIGSAAVPPTLAIADVALETQTVQLAISP----IETAEGKDAW 129 Query: 131 VFKTPQNDIMMSLAIARDMVARGYQRIAFIGYSNAYGEGWYEEFKKAAGLKKLELVANER 190 F+ PQ+ +M+ + M G + I F+GYS++YGE W +E ++ A +++ ER Sbjct: 130 TFRLPQSTAVMAGGVVNQMKKLGVKTIGFLGYSDSYGENWLKEVQRLATAAGMKMGTVER 189 Query: 191 FAPTDSSTTAQALKLVAAKPDAILIAASGTPAVVPQRALKERGFKGQIYQTHGVANADFL 250 F+ D+S TAQAL++V++ PDA+L+ ASG+ A +P +AL ERG+KG+I+QTH A+ D + Sbjct: 190 FSRADTSVTAQALRVVSSNPDAVLVVASGSGAAMPHKALIERGYKGKIFQTHSAASRDLI 249 Query: 251 RVGGKMLDGTLLPAGPVLVAAQLPASNPIRKVALDYTTKYEAVHGKDSVSLFGGYAWDAG 310 R+GGK ++G + +GP +V LP SN +K+A+D+ T+YE G S +LF + +DA Sbjct: 250 RLGGKDVEGAYVISGPAVVPEALPDSNASKKLAMDFVTRYEKQFGAGSRNLFAAHTYDAQ 309 Query: 311 VLLRWAIPVALKGAKPGTPEFRAALRDALEATKEVTGVHGVYTMSSTDHLGLDQRAAVMV 370 ++L+ +P ALK AKPGTP FRAAL++ALE + + G S DH GL A +M+ Sbjct: 310 LVLQKVVPEALKKAKPGTPAFRAALKEALETSGPIQITQGTLNYSPKDHFGLGDDARIML 369 Query: 371 KIVDGAWQ 378 I +G W+ Sbjct: 370 TIQNGNWK 377 >gi|300312236|ref|YP_003776328.1| amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 385 Score = 69.7 bits (169), Expect = 4e-13, Method: Compositional matrix adjust. Identities = 96/390 (24%), Positives = 162/390 (41%), Gaps = 46/390 (11%) Query: 7 LLALALAGCALVARADINVGVTVSTTGPAASLGIPEKNTIALLPTTIA------GHKVNY 60 L+ L+ AL A DI +GV + TGPAA G+ KN L I G K+ Sbjct: 14 LIGATLSQAALAA--DIKLGVAEALTGPAAKYGVAIKNGFTLASEEINAKGGVNGDKLAL 71 Query: 61 IILDDASDTTQAVKNARRFVSENNVDVIVGSSVSPNSISMIEVAADAQTPMISLAAAARI 120 +I D+ +A+ ++ + ++ V + G ++S ++ + +A A+ + + A Sbjct: 72 VIEDEQGKKEEAINVFKKLIFQDKVLAVFGPTLSNSAFAADPIANAAKVVVFGTSNTADG 131 Query: 121 VEPMDAKKKWVFKTPQNDIM----MSLAIARDMVARGYQRIAFI-GYSNAYGEGWYEEFK 175 + M + F+ N +M + + + + G +++A I G +A+ + Y+ FK Sbjct: 132 ITAMG---PFTFR---NSVMEADVLPVTVKAAVKHFGIKKVAVIYGNDDAFTKSGYDVFK 185 Query: 176 KAAGLKKLELVANERFAPTDSSTTAQALKLVAAKPDAILIAASGTPA---VVPQRALKER 232 +K+ + E +A D AQ K+ A PDAI+ + A ++ RAL Sbjct: 186 ATLEHQKIPVTTTEAYAKGDVDFKAQLTKIKAGNPDAIVCSCLAEEAANIILQTRAL--- 242 Query: 233 GFKGQIYQTHGVANADFLRVGGKMLDGTLLPAGPVLVAAQLPASNPIRKVALDYTTKYEA 292 G K +G+ + + D TL+ P Q PA+ + T Y+A Sbjct: 243 GMKQPFIGGNGLNSPKLFEIAKDAGDNTLM-GSPWSAENQTPANKA-------FITAYKA 294 Query: 293 VHGKDSVSLFGGYAWDAGVLLRWAIPVALKGAK--PGTPEFRAALRDALEATKEVTGVHG 350 G D F A+DA + + ALK K + R ALR AL A K + G G Sbjct: 295 KFGADP-DQFAAQAYDA----MYIVADALKNVKLSGNLAKDRDALRAALPAVK-IDGATG 348 Query: 351 VYTMSST-----DHLGLDQRAAVMVKIVDG 375 + + +G D +V I G Sbjct: 349 KFAFRAAPAVAGKQVGFDADQEAIVNIAKG 378 >gi|300311113|ref|YP_003775205.1| branched-chain amino acid ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 383 Score = 67.4 bits (163), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 93/385 (24%), Positives = 169/385 (43%), Gaps = 27/385 (7%) Query: 5 RPLLALALAGCALVARA--DINVGVTVSTTGPAASLGIPEKNTIALLPTTIA------GH 56 + +A AL AL ++A I +GV+ TG +A +G+ ++ + L I G Sbjct: 6 QAFVAAALMSLALTSQAADPIKIGVSGPYTGGSAPMGVSMRDGVKLAVAEINAAGGLLGR 65 Query: 57 KVNYIILDDASDTTQAVKNARRFVSENNVDVIVGSSVSPNSISMIEVAADAQTPMISLAA 116 ++ I DD + + V+ A+ +++ V VG + +++ +A+ P+I+ A Sbjct: 66 QIQLIERDDEAKNERGVQIAQELINKEKVVATVGFINTGVALASQRFYQEAKIPVINNVA 125 Query: 117 AARIVEPMDAKKK--WVFKTPQNDIMMSLAIARDMVAR-GYQRIAFIGYSNAYGEGWYEE 173 ++ K+K +VF+T D + + IA + V + + ++A + S YG+ E+ Sbjct: 126 TGTVITKQFEKEKDNYVFRTSAADKIQAKMIADEAVDKQKFTKVAILADSTNYGQLGRED 185 Query: 174 FKKAAGLKKLELVANERFAPTDSSTTAQALKLVAAKPDAILIAASGTPAVVPQRALKERG 233 +K KK+ VA E++ D TAQ LK A+L G +++ Sbjct: 186 LEKVLADKKVTPVAVEKYNIKDVDMTAQLLKSKQGGAQAVLTYGIGPELAQIANGMEKLN 245 Query: 234 FKGQIYQTHGVANADFLRVGGKMLDGTLLPAGPVLVAAQLPASNPIRKVALD-YTTKYEA 292 + I + ++ +F+ GK +G +P + + P RK ++ Y Y+ Sbjct: 246 WHVPIIGSWPLSMGNFIDNSGKNGNGARMPQTFI-----QDGNTPKRKAFIEAYQKAYKV 300 Query: 293 VHGKDSVSLFGGYAWDAGVLLRWAIPVALKGAKPGTPEFRAALRDALEATKEVTGVHGVY 352 +VS GY D+ +LL AI A G G + RAAL + +V GV Y Sbjct: 301 DRMPSAVSAAQGY--DSILLLAAAIKQA--GTTDG-DKVRAALEN---LNTKVEGVVTTY 352 Query: 353 T--MSSTDHLGLDQRAAVMVKIVDG 375 + DH + VM ++ +G Sbjct: 353 DKPFTHDDHEAIKPAMVVMGEVKNG 377 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.132 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,164,610 Number of extensions: 49161 Number of successful extensions: 210 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 210 Number of HSP's successfully gapped: 11 Length of query: 381 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 294 Effective length of database: 1,207,925 Effective search space: 355129950 Effective search space used: 355129950 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)