BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0125.p (686 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313407|ref|YP_003777499.1| ring-opening enzyme protein [He... 1381 0.0 gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrog... 108 1e-24 gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde ... 98 2e-21 gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semial... 93 9e-20 gi|300310355|ref|YP_003774447.1| NAD-dependent aldehyde dehydrog... 92 1e-19 >gi|300313407|ref|YP_003777499.1| ring-opening enzyme protein [Herbaspirillum seropedicae SmR1] Length = 686 Score = 1381 bits (3575), Expect = 0.0, Method: Compositional matrix adjust. Identities = 683/686 (99%), Positives = 683/686 (99%) Query: 1 MTAHPPLLQSFIAGRWLGQRPSQALHSAVNGSAVASTHEESIDFGEALEHARKTGLPALM 60 MTAHPPLLQSFIAGRWLGQRPSQALHSAVNGSAVASTHEESIDFGEALEHARKTGLPALM Sbjct: 1 MTAHPPLLQSFIAGRWLGQRPSQALHSAVNGSAVASTHEESIDFGEALEHARKTGLPALM 60 Query: 61 ALDFQQRAAILKALAKYLMEHKEVLYAVSAHTGATRADSWIDIEGGSGTLFTYASIGANE 120 ALDFQQRAAILKALAKYLMEHKEVLYAVSAHTGATRADSWIDIEGGSGTLFTYASIGANE Sbjct: 61 ALDFQQRAAILKALAKYLMEHKEVLYAVSAHTGATRADSWIDIEGGSGTLFTYASIGANE 120 Query: 121 FPSSNLVHEGPAMPLGKQGRFAGTHILVPRGGVAVHINAFNFPVWGMLEKFAPTFLAGMP 180 FPSSNLVHEGPAM LGKQGRFAGTHILVPRGGVAVHINAFNFPVWGMLEKFAPTFLAGMP Sbjct: 121 FPSSNLVHEGPAMRLGKQGRFAGTHILVPRGGVAVHINAFNFPVWGMLEKFAPTFLAGMP 180 Query: 181 CIVKPATATSYLAEAAVRLIQQSGLLPEGSLQLVIGSTGDLLERLQAQDVVTFTGSADTA 240 CIVKPATATSYLAEAAVRLIQQSGLLPEGSLQLVIGSTGDLLERLQAQDVVTFTGSADTA Sbjct: 181 CIVKPATATSYLAEAAVRLIQQSGLLPEGSLQLVIGSTGDLLERLQAQDVVTFTGSADTA 240 Query: 241 AKLRAHPNLIANSIPFNAEADSLNCAILGPDITADDEEFDLFVKEVAREMTTKAGQKCTA 300 AKLRAHPNLIANSIPFNAEADSLNCAILGPDITADDEEFDLFVKEVAREMTTKAGQKCTA Sbjct: 241 AKLRAHPNLIANSIPFNAEADSLNCAILGPDITADDEEFDLFVKEVAREMTTKAGQKCTA 300 Query: 301 IRRAIVPRRHVDAVAERLAARLSKIVVGDPALEAVRMGPLASRAQQRDVEERVALLRRSA 360 IRRAIVPRRHVDAVAERLAARLSKIVVGDPALE VRMGPLASRAQQRDVEERVALLR SA Sbjct: 301 IRRAIVPRRHVDAVAERLAARLSKIVVGDPALETVRMGPLASRAQQRDVEERVALLRHSA 360 Query: 361 DLILGAGSAFAPQGQGVGEGAFFAPTLLLARDTAAGSPVHEVEAFGPVSTLMPYDDLDEA 420 DLILGAGSAFAPQGQGVGEGAFFAPTLLLARDTAAGSPVHEVEAFGPVSTLMPYDDLDEA Sbjct: 361 DLILGAGSAFAPQGQGVGEGAFFAPTLLLARDTAAGSPVHEVEAFGPVSTLMPYDDLDEA 420 Query: 421 LALAARGRGSLVASLVTRTPAVAARAIPVAAAWHGRLLVLDREAAAESTGHGSPLPQLKH 480 LALAARGRGSLVASLVTRTPAVAARAIPVAAAWHGRLLVLDREAAAESTGHGSPLPQLKH Sbjct: 421 LALAARGRGSLVASLVTRTPAVAARAIPVAAAWHGRLLVLDREAAAESTGHGSPLPQLKH 480 Query: 481 GGPGRAGGGEELGGSRAVKHYLQRTAVQGSPTMLAAVVGEHVRGARVRESGIHPFRHYFE 540 GGPGRAGGGEELGGSRAVKHYLQRTAVQGSPTMLAAVVGEHVRGARVRESGIHPFRHYFE Sbjct: 481 GGPGRAGGGEELGGSRAVKHYLQRTAVQGSPTMLAAVVGEHVRGARVRESGIHPFRHYFE 540 Query: 541 DLQIGDSLTTHRRTVTEADIVNFGCLSGDHFYMHFDEIAARQSPFGKRIAHGYFVLSAAA 600 DLQIGDSLTTHRRTVTEADIVNFGCLSGDHFYMHFDEIAARQSPFGKRIAHGYFVLSAAA Sbjct: 541 DLQIGDSLTTHRRTVTEADIVNFGCLSGDHFYMHFDEIAARQSPFGKRIAHGYFVLSAAA 600 Query: 601 GLFVSPAPGPVLANYGLDTLRFVKPVGIGDTISARLTCKRKIDRNKKDANGQGQGVVAWD 660 GLFVSPAPGPVLANYGLDTLRFVKPVGIGDTISARLTCKRKIDRNKKDANGQGQGVVAWD Sbjct: 601 GLFVSPAPGPVLANYGLDTLRFVKPVGIGDTISARLTCKRKIDRNKKDANGQGQGVVAWD 660 Query: 661 VEVSNQEGELVASYDILTLVAKKPLE 686 VEVSNQEGELVASYDILTLVAKKPLE Sbjct: 661 VEVSNQEGELVASYDILTLVAKKPLE 686 >gi|300311569|ref|YP_003775661.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 477 Score = 108 bits (271), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 142/514 (27%), Positives = 213/514 (41%), Gaps = 65/514 (12%) Query: 7 LLQSFIAGRWLGQRPSQAL-----HSAVNGSAVASTHEESIDFGEALEHARKTGLPALMA 61 + Q FIA RW+ +++ A+A + ID +A+ AR A Sbjct: 1 MKQHFIANRWVAPTSGESIPVLDPSDGQPFDAIARGNATDID--QAVRAARLAYEGAWGR 58 Query: 62 LDFQQRAAILKALAKYLMEHKEVLYAVSAHTGAT-----RADSWIDIEGGSGTLFTYASI 116 L +R+ +L LA L +H+E L + RAD+ + F + + Sbjct: 59 LSAAERSRLLMKLAYKLQDHQEELAQLEGRDCGKPMKQARADAAAIVR-----YFEFYAG 113 Query: 117 GANEFPSSNLVHEGPAMPLGKQGRFAGTHILVPRG--GVAVHINAFNFPVWGMLEKFAPT 174 A++ + ++ AG +L R GV HI +N+P+ Sbjct: 114 AADKLHGDTIPYQ------------AGYTVLTLREPHGVTGHIVPWNYPMQIFGRCVGGA 161 Query: 175 FLAGMPCIVKPATATSYLAEAAVRLIQQSGLLPEGSLQLVIG---STGDLLERLQAQDVV 231 AG C+VKPA L + G PEG+L +V G GDLL + + + Sbjct: 162 LAAGNTCVVKPAEDACLSILRVAELAAEVGF-PEGALNIVTGYGHEAGDLLVKHPDVNHI 220 Query: 232 TFTGSADTAAKLRAHPNLIANSIPFNAEADSLNCAILGPDITADDEEFDLFVKEVAREMT 291 +FTGS + L+ + + +L P I +D + + + V + Sbjct: 221 SFTGSPNVG-------KLVVRAAAEHHTPVTLELGGKSPQIVFEDADMEAMLPVVVNAIV 273 Query: 292 TKAGQKCTAIRRAIVPRRHVDAVAERLAARLSKIVVGDPALEAVRMGPLASRAQQRDVEE 351 AGQ C+A R +V R + V ERL+ S++ VG ++ + GPL + QQ E Sbjct: 274 QNAGQTCSAGSRLLVQRGAYEQVLERLSGLFSQLQVGSSQMD-LDCGPLIRKTQQ----E 328 Query: 352 RVALLRRSADLILGAGSAFAPQGQ---GVGEGAFF-APTLLLARDTAAGSPVHEVEAFGP 407 RVA A G A A QG+ GV G F+ AP LL RD + + E FGP Sbjct: 329 RVAGFLSDAQR---DGIALAAQGKIAAGVPTGGFYQAPALL--RDVPPRHRLAQEEVFGP 383 Query: 408 VSTLMPYDDLDEALALAARGRGSLVASLVTRTPAVAARAIPVAAAWHGRLLVLDREAAAE 467 V MP++D A+ALA LVAS+ TR R + +A A + ++ A Sbjct: 384 VLAAMPFEDEAHAIALANGTDYGLVASVWTRD---GGRQMRLARAIRSGQVFINNYGA-- 438 Query: 468 STGHGSPLP--QLKHGGPGRAGGGEELGGSRAVK 499 G G LP +K G GR G E L G +K Sbjct: 439 --GGGVELPFGGVKASGHGREKGFEALYGFTVLK 470 >gi|300310471|ref|YP_003774563.1| NAD-dependent betaine aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 486 Score = 98.2 bits (243), Expect = 2e-21, Method: Compositional matrix adjust. Identities = 111/437 (25%), Positives = 186/437 (42%), Gaps = 67/437 (15%) Query: 11 FIAGRWLGQRPSQALHSAVNGSAVASTHEESI---------DFGEALEHARKTGLPALMA 61 +I G+W Q P + +AV VA EE + D +A++ AR+ +PA A Sbjct: 19 YIDGKW--QPPQGSERAAV----VAPATEERVCEIALGNAADVDQAVQAARR-AIPAWAA 71 Query: 62 LDFQQRAAILKALAKYLMEHKEVL-YAVSAHTGATRADSWIDIEGGSGTLFTYASIGANE 120 +RAA+L + L+E E+ A++ GA +YA Sbjct: 72 TSPLERAALLGRVHALLLERSELFAQALTLEMGAP---------------ISYARTAHVP 116 Query: 121 FPSSNLVHEGPAMPLGKQGRFAGTHILVPRG---------GVAVHINAFNFPVWGMLEKF 171 + +L + A + PRG GV I +N+P++ + K Sbjct: 117 LAAEHL--------RVARDNLADYPFIRPRGTTAIVREPIGVCALITPWNWPIYQITAKV 168 Query: 172 APTFLAGMPCIVKPATATSYLAEAAVRLIQQSGLLPEGSLQLVIGSTGDLLERLQAQ--- 228 P AG ++KP+ + A ++ ++G+ P G LV GS ++ L A Sbjct: 169 GPALAAGCTVVLKPSELSPLSALLFAEIVHEAGV-PAGVFNLVNGSGAEVGAALSAHPQV 227 Query: 229 DVVTFTGSADTAAKLRAHPNLIANSIPFNAEADSLNCAILGPDITADDEEFDLFVKEVAR 288 D+++ TGS L+A + + + P++ D + + + Sbjct: 228 DMISITGSTRAGV-------LVAQAAAVTVKRVAQELGGKSPNLVLPDADLERAIPPGVA 280 Query: 289 EMTTKAGQKCTAIRRAIVPRRHVDAVAERLAARLSKIVVGDPALEAVRMGPLASRAQQRD 348 GQ C+A R IVPR + V E A L+++ VGDP A GPLA+RAQ Sbjct: 281 AALRNMGQSCSAPTRLIVPRAALARVHELALATLAQMKVGDPNDAATTHGPLANRAQFLR 340 Query: 349 VEERV-ALLRRSADLILGAGSAFAPQGQGVGEGAFFAPTLLLARDTAAGSPVHEVEAFGP 407 V++ + A L+ A L+ G + G+ +G + PT+ + + + E FGP Sbjct: 341 VQQMIEAGLQDGARLLAGGPG----RPDGLAQGYYARPTIF--SEVHTDMVIAQEEIFGP 394 Query: 408 VSTLMPYDDLDEALALA 424 V ++PYD ++EA+A+A Sbjct: 395 VLAILPYDTVEEAIAIA 411 >gi|300310410|ref|YP_003774502.1| NADP-dependent succinate-semialdehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 484 Score = 92.8 bits (229), Expect = 9e-20, Method: Compositional matrix adjust. Identities = 120/454 (26%), Positives = 188/454 (41%), Gaps = 58/454 (12%) Query: 5 PPLL--QSFIAGRWLG--QRPSQALHSAVNGSAVASTHEESIDFGE---ALEHARKTGLP 57 P LL Q+F+ G+W Q A+H+ NG + H + E A+E A P Sbjct: 7 PALLRQQAFVNGQWCDADQGERLAVHNPANGELLG--HVPLMGAAETRRAIEAA-NAAWP 63 Query: 58 ALMALDFQQRAAILKALAKYLMEHKEVLYAV-SAHTGATRADSWIDIEGGSGTLFTYASI 116 A ++R+AIL+ + ++ + + L + +A G A++ +I + + +A Sbjct: 64 AWKKKTAKERSAILRRWYELMLANTDDLALIMTAEQGKPLAEARGEIGYAASFIEWFAEE 123 Query: 117 GANEF--------PSSNLVHEGPAMPLGKQGRFAGTHILVPRGGVAVHINAFNFPVWGML 168 G + PS+ +V A+ GV I +NFP + Sbjct: 124 GKRTYGDTIPSPSPSNRIVVIKEAI------------------GVCAAITPWNFPAAMIT 165 Query: 169 EKFAPTFLAGMPCIVKPATATSYLAEAAVRLIQQSGLLPEGSLQLVIGSTGDLLERLQAQ 228 K P AG P ++KPA AT + A A L +++G+ P G +V G+ + + + Sbjct: 166 RKAGPALAAGCPMVLKPAEATPFSALALAVLAERAGI-PAGVFSVVTGTPKGIGGEMTSN 224 Query: 229 DVV---TFTGSADTAAKLRAHPNLIANSIPFNAEADSLNCAILGPDITADDEEFDLFVKE 285 +V +FTGS L A+SI + SL P I DD + D V+ Sbjct: 225 PIVRKISFTGSTGVGKLLMQQS---ASSI----KKLSLELGGNAPFIVFDDADLDAAVEG 277 Query: 286 VAREMTTKAGQKCTAIRRAIVPRRHVDAVAERLAARLSKIVVGDPALEAVRMGPLASRAQ 345 AGQ C R V DA A +L + K VG E V GPL + Sbjct: 278 AIASKYRNAGQTCVCANRIYVQDGVYDAFAAKLVEAVKKFKVGQGTEEGVTQGPLINEQA 337 Query: 346 QRDVEERVA-LLRRSADLILGAGSAFAPQGQGVGEGAFFAPTLLLARDTAAGSPVHEVEA 404 + VE+ VA + + A ++LG + +G +FF PT+L D V E Sbjct: 338 VQKVEQHVADAVAKGARVLLGG------KRHALGH-SFFEPTVL--ADVTPAMQVAREET 388 Query: 405 FGPVSTLMPYDDLDEALALAARGRGSLVASLVTR 438 FGP++ L + +E LALA L + +R Sbjct: 389 FGPMAPLFRFKTDEEVLALANDTEFGLASYFYSR 422 >gi|300310355|ref|YP_003774447.1| NAD-dependent aldehyde dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 793 Score = 92.4 bits (228), Expect = 1e-19, Method: Compositional matrix adjust. Identities = 124/459 (27%), Positives = 179/459 (38%), Gaps = 55/459 (11%) Query: 1 MTAHPPLLQSFIAGRWLGQRPSQALHSAVNGSAVASTHEESIDFGEALEHARKTGLPALM 60 + +H FIAGR+ + +G +A + S +A A + LP Sbjct: 26 LASHEASFGHFIAGRFTQPKADLDDIDPASGKLLAHLSQGSAADVDAAVQAAQAALPGWQ 85 Query: 61 ALDFQQRAAILKALAKYLMEHKEVLYAVSAHTGAT--RADSWIDIEGGSGTLFTYA---S 115 AL RA L ALA+ + H +L + R +D+ + + YA Sbjct: 86 ALGGHGRARHLYALARTVQRHARLLAVLETLDNGKPLRESRDVDVPLVARHFYHYAGWAQ 145 Query: 116 IGANEFPSSNLVHEGPAMPLGKQGRFAGTHILVPRGGVAVHINAFNFPVWGMLEKFAPTF 175 + +EFP H+ V GV I +NFP+ + K AP Sbjct: 146 LQESEFPD---------------------HVPV---GVVGQIVPWNFPLLMLAWKIAPAL 181 Query: 176 LAGMPCIVKPATATSYLAEAAVRLIQQSGLLPEGSLQLVIG--STGDLLERLQAQDVVTF 233 G ++KPA TS A L QQ+GL P G L +V G +TG + + F Sbjct: 182 ALGNTVVLKPAENTSLTALLFAELAQQAGL-PAGVLNIVTGDGATGAAVVAHPGIQKIAF 240 Query: 234 TGSADTAAKLRAHPNLIANSIPFNAEADSLNCAILGPDITADDEEFDLFVKEVAREMTTK 293 TGS + LI + ++ +L P I +D + D ++ V + Sbjct: 241 TGSTEVG-------RLIREQTAGSGKSLTLELGGKSPFIVFEDADIDAAIEGVVDAIWFN 293 Query: 294 AGQKCTAIRRAIVPRRHVDAVAERLAARLSKIVVGDPALEAVRMGPLASRAQ----QRDV 349 GQ C A R +V D RL R+ K+ VG P + MG L S Q + V Sbjct: 294 QGQVCCAGSRLLVQEGIHDLFIARLKTRMQKLKVGAPLDKCSDMGALISPVQLERVRSLV 353 Query: 350 EERVALLRRSADLILGAGSAFAPQGQGVGEGAFFAPTLLLARDTAAGSPVHEVEAFGPVS 409 E+ V + ++L P G G F+ PTLL AA V E FGPV Sbjct: 354 EQGVREGAQCHQVVLD-----TPPG-----GCFYPPTLLTGVHPAA--TVASEEIFGPVL 401 Query: 410 TLMPYDDLDEALALAARGRGSLVASLVTRTPAVAARAIP 448 M + DEA+ LA R L AS+ + T +A P Sbjct: 402 VAMSFRTPDEAVQLANNSRYGLAASIWSETIGLALGVAP 440 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,122,161 Number of extensions: 92904 Number of successful extensions: 392 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 368 Number of HSP's successfully gapped: 19 Length of query: 686 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 594 Effective length of database: 1,184,250 Effective search space: 703444500 Effective search space used: 703444500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 134 (56.2 bits)