BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0117.p (462 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313399|ref|YP_003777491.1| acyl-CoA carboxylase biotin sub... 920 0.0 gi|300309729|ref|YP_003773821.1| biotin carboxylase [Herbaspiril... 418 e-118 gi|300313973|ref|YP_003778065.1| acetyl/propionyl-CoA carboxylas... 352 5e-98 gi|300313753|ref|YP_003777845.1| urea amidolyase [Herbaspirillum... 339 2e-94 gi|300310711|ref|YP_003774803.1| carbamoyl-phosphate synthase la... 58 2e-09 >gi|300313399|ref|YP_003777491.1| acyl-CoA carboxylase biotin subunit [Herbaspirillum seropedicae SmR1] Length = 462 Score = 920 bits (2378), Expect = 0.0, Method: Compositional matrix adjust. Identities = 458/462 (99%), Positives = 461/462 (99%) Query: 1 MTTSLRKLLIANRGEIAVRIIRAAQALGITTVAACSDADTDSLAARMADEVQRIGPARAD 60 MTTSLRKLLIANRGEIAVRIIRAAQALGITTVAACSDADTDSLAARMADEVQRIGPARAD Sbjct: 1 MTTSLRKLLIANRGEIAVRIIRAAQALGITTVAACSDADTDSLAARMADEVQRIGPARAD 60 Query: 61 RSYLNVDALLKAARDSGADALHPGYGFLSENAAFAEAVNAAGLIFVGPQADTIRRMGDKA 120 RSYLNVDALLKAARDSGADALHPGYGFLSENAAFAEAVNAAGLIFVGPQADTIRRMGDKA Sbjct: 61 RSYLNVDALLKAARDSGADALHPGYGFLSENAAFAEAVNAAGLIFVGPQADTIRRMGDKA 120 Query: 121 EARRTAAAAGVPVVPGSAGELEDIATALACAEEIGYPLLIKASAGGGGRGIRMARDATEL 180 EARRTAAAAGVPVVPGSAGELED+ATALACA+EIGYPLLIKASAGGGGRGIRMARDATEL Sbjct: 121 EARRTAAAAGVPVVPGSAGELEDLATALACADEIGYPLLIKASAGGGGRGIRMARDATEL 180 Query: 181 AREFPLAQAEAQAAFGSAAVYLERFIRRARHIEVQILGDGERAVHLFERECSLQRRRQKV 240 AREFPLAQAEAQAAFGSAAVYLERFIRRARHIEVQILGDGERAVHLFERECSLQRRRQKV Sbjct: 181 AREFPLAQAEAQAAFGSAAVYLERFIRRARHIEVQILGDGERAVHLFERECSLQRRRQKV 240 Query: 241 LEEAPSPALTPAQRQALCDSAVRLAEQLHYRGAGTLEYLFDDESGEFFFIEMNTRIQVEH 300 LEEAPSPALTPAQRQALCDSAVRLAE+LHYRGAGTLEYLFDDESGEFFFIEMNTRIQVEH Sbjct: 241 LEEAPSPALTPAQRQALCDSAVRLAERLHYRGAGTLEYLFDDESGEFFFIEMNTRIQVEH 300 Query: 301 PVTEMITGIDLVQAMLRIAGGEPLGLQQSDIRMHGAALEMRINAEDPERNFFPCPGTVAE 360 PVTEMITGIDLVQAMLRIAGGEPLGLQQSDIRM GAALEMRINAEDPERNFFPCPGTVAE Sbjct: 301 PVTEMITGIDLVQAMLRIAGGEPLGLQQSDIRMQGAALEMRINAEDPERNFFPCPGTVAE 360 Query: 361 LQWPQGEGIRVESHLYAGYRIPPYYDSLLAKLVVHGKDRAQAIARAQAAVLATRITGMAT 420 LQWPQGEGIRVESHLYAGYRIPPYYDSLLAKLVVHGKDRAQAIARAQAAVLATRITGMAT Sbjct: 361 LQWPQGEGIRVESHLYAGYRIPPYYDSLLAKLVVHGKDRAQAIARAQAAVLATRITGMAT 420 Query: 421 TLSLHQWLLADARVQSARFDTGALETWLAERTDTRAKQLEEA 462 TLSLHQWLLADARVQSARFDTGALETWLAERTDTRAKQLEEA Sbjct: 421 TLSLHQWLLADARVQSARFDTGALETWLAERTDTRAKQLEEA 462 >gi|300309729|ref|YP_003773821.1| biotin carboxylase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 418 bits (1075), Expect = e-118, Method: Compositional matrix adjust. Identities = 219/449 (48%), Positives = 300/449 (66%), Gaps = 5/449 (1%) Query: 5 LRKLLIANRGEIAVRIIRAAQALGITTVAACSDADTDSLAARMADEVQRIGPARADRSYL 64 K+LIANRGEIA+RI RA + LGI TV S+AD ++ ++ADE IGPA + SYL Sbjct: 2 FEKILIANRGEIALRIQRACRELGIKTVVVHSEADREAKYVKLADESVCIGPAPSTLSYL 61 Query: 65 NVDALLKAARDSGADALHPGYGFLSENAAFAEAVNAAGLIFVGPQADTIRRMGDKAEARR 124 N+ A++ AA + A A+HPGYGFLSENA FAE V +G +F+GP+A+ IR MGDK A++ Sbjct: 62 NMPAIISAAEVTDAQAIHPGYGFLSENADFAERVEKSGFVFIGPRAENIRMMGDKVSAKQ 121 Query: 125 TAAAAGVPVVPGSAGELEDIATALA-CAEEIGYPLLIKASAGGGGRGIRMARDATELARE 183 AGVP VPGS G L D + A +IGYP++IKA+ GGGGRG+R+ L Sbjct: 122 AMIRAGVPCVPGSDGALPDNPKEIVQIARKIGYPVIIKAAGGGGGRGMRVVHTEAALINA 181 Query: 184 FPLAQAEAQAAFGSAAVYLERFIRRARHIEVQILGDGER-AVHLFERECSLQRRRQKVLE 242 + + EA AAFG+ VY+E+++ RH+E+QIL D + A+ L ER+CS+QRR QKV+E Sbjct: 182 VTMTKTEAGAAFGNPEVYMEKYLENPRHVEIQILADEHKQAIWLGERDCSMQRRHQKVIE 241 Query: 243 EAPSPALTPAQRQALCDSAVRLAEQLHYRGAGTLEYLFDDESGEFFFIEMNTRIQVEHPV 302 EAP+P + + + + +++YRGAGT E+L+++E EF+FIEMNTR+QVEHPV Sbjct: 242 EAPAPGIPRKIIEKIGERCAEACRKMNYRGAGTFEFLYENE--EFYFIEMNTRVQVEHPV 299 Query: 303 TEMITGIDLVQAMLRIAGGEPLGLQQSDIRMHGAALEMRINAEDPERNFFPCPGTVAELQ 362 TEMITG+D+VQ +RIA GE L +Q DI + G A+E RINAEDP + F P PG + Sbjct: 300 TEMITGVDIVQEQIRIAAGEKLRYRQRDIELKGHAIECRINAEDPFK-FIPSPGRITAWH 358 Query: 363 WPQGEGIRVESHLYAGYRIPPYYDSLLAKLVVHGKDRAQAIARAQAAVLATRITGMATTL 422 P G GIRV+SH Y+GY +PP YDS++ K++ +G R QAI R Q A+ + G++T + Sbjct: 359 VPGGPGIRVDSHAYSGYFVPPNYDSMVGKVIAYGATREQAIRRMQIALSEMVVEGISTNI 418 Query: 423 SLHQWLLADARVQSARFDTGALETWLAER 451 LH+ L+ DAR + LE L+ER Sbjct: 419 PLHRELMVDARFFEGGTNIHYLEHKLSER 447 >gi|300313973|ref|YP_003778065.1| acetyl/propionyl-CoA carboxylase subunit alpha [Herbaspirillum seropedicae SmR1] Length = 671 Score = 352 bits (902), Expect = 5e-98, Method: Compositional matrix adjust. Identities = 207/453 (45%), Positives = 294/453 (64%), Gaps = 15/453 (3%) Query: 7 KLLIANRGEIAVRIIRAAQALGITTVAACSDADTDSLAARMADEVQRIGPARADRSYLNV 66 K+LIANRGEIA R+ A+ LGI TVA SDAD + DE R+GPA A SYL Sbjct: 4 KILIANRGEIACRVAATARRLGIATVAVYSDADARAKHVAACDESVRLGPAPARESYLCA 63 Query: 67 DALLKAARDSGADALHPGYGFLSENAAFAEAVNAAGLIFVGPQADTIRRMGDKAEARRTA 126 + ++ AA +GA A+HPGYGFLSENA FA+A AGL+F+GP A I MG KA A+ Sbjct: 64 EKIIAAALATGAQAIHPGYGFLSENAGFAQACAQAGLVFIGPPASAIEAMGSKAAAKALM 123 Query: 127 AAAGVPVVPGSAGELEDIATALACAEEIGYPLLIKASAGGGGRGIRMARDATELAREFPL 186 A VP+VPG GE ++ + A+ IGYP+L+KASAGGGG+G+R+ + + Sbjct: 124 EQAAVPLVPGYHGEEQESGLLHSQADRIGYPVLLKASAGGGGKGMRVVERSEDFDAALAS 183 Query: 187 AQAEAQAAFGSAAVYLERFIRRARHIEVQILGDGE-RAVHLFERECSLQRRRQKVLEEAP 245 + EA A+FG V +E+++ R RHIE+Q+ DG V+LFER+CS+QRR QKVLEEAP Sbjct: 184 CKREAIASFGDDRVLVEKYLTRPRHIEIQVFADGHGHCVYLFERDCSVQRRHQKVLEEAP 243 Query: 246 SPALTPAQRQALCDSAVRLAEQLHYRGAGTLEYLFDDESGEFFFIEMNTRIQVEHPVTEM 305 +P ++ +R A+ ++AV A + Y GAGT+E++ ++ G F+F+EMNTR+QVEHPVTEM Sbjct: 244 APGMSEERRAAMGEAAVAAARAVGYVGAGTVEFI-ANQDGSFYFMEMNTRLQVEHPVTEM 302 Query: 306 ITGIDLVQAMLRIAGGEPLGLQQSDIRMHGAALEMRINAEDPERNFFPCPGTV------A 359 ITG+DLV+ LR+A G+ L L+Q +R+HG A+E RI AE+PE+ F P GT+ A Sbjct: 303 ITGLDLVEWQLRVAAGQALPLKQEQLRIHGHAIEARIYAENPEKGFLPAIGTLTRMRSAA 362 Query: 360 ELQWPQGEG-------IRVESHLYAGYRIPPYYDSLLAKLVVHGKDRAQAIARAQAAVLA 412 +++ G+ +R+++ + G I P+YD ++AKL+V G DR A+ Q A+ Sbjct: 363 AVEFRLGQSAPGDPAHVRIDAGVREGDAISPFYDPMIAKLIVWGPDRDAALRNMQRALAQ 422 Query: 413 TRITGMATTLSLHQWLLADARVQSARFDTGALE 445 ++ G+AT ++ Q L+A +A DTG +E Sbjct: 423 YQVVGLATNIAFLQRLVAGQAFSTAALDTGLIE 455 >gi|300313753|ref|YP_003777845.1| urea amidolyase [Herbaspirillum seropedicae SmR1] Length = 1207 Score = 339 bits (870), Expect = 2e-94, Method: Compositional matrix adjust. Identities = 205/452 (45%), Positives = 286/452 (63%), Gaps = 7/452 (1%) Query: 8 LLIANRGEIAVRIIRAAQALGITTVAACSDADTDSLAARMADEVQRIGPARADRSYLNVD 67 LLIANRG IA RI+R + L +T V S+AD SL AD +G A ++YL VD Sbjct: 5 LLIANRGAIACRILRTLRTLDVTGVCVYSEADLGSLHVLQADRAISLGEGPAAQTYLAVD 64 Query: 68 ALLKAARDSGADALHPGYGFLSENAAFAEAVNAAGLIFVGPQADTIRRMGDKAEARRTAA 127 +L AARDSGA A+HPGYGFLSENAAFAEA AAG+ FVGP +R G K AR A Sbjct: 65 KILAAARDSGAQAIHPGYGFLSENAAFAEACEAAGIAFVGPTPAQLRIFGLKHTARALAR 124 Query: 128 AAGVPVVPGSAGELEDIATALACAEEIGYPLLIKASAGGGGRGIRMARDATELAREFPLA 187 GVP++ G+ LE I ALA A +GYP+++K++AGGGG G+R+ R ELA F Sbjct: 125 EQGVPMLEGT-DLLESIDDALAAASRVGYPVMLKSTAGGGGIGMRVCRSDAELADAFDSV 183 Query: 188 QAEAQAAFGSAAVYLERFIRRARHIEVQILGDGE-RAVHLFERECSLQRRRQKVLEEAPS 246 + Q F A V++E++I RARH+EVQ+ GDG + L R+CS+QRR QKVLEE P+ Sbjct: 184 RRLGQNNFSDAGVFIEKYIERARHLEVQVFGDGRGEVIALGVRDCSVQRRNQKVLEETPA 243 Query: 247 PALTPAQRQALCDSAVRLAEQLHYRGAGTLEYLFDDESGEFFFIEMNTRIQVEHPVTEMI 306 P L +ALC +A++LA+ ++YR AGT+E+++D +G+F+F+E+NTR+QVEH VTE + Sbjct: 244 PNLPDGMAEALCAAAIKLAKAVNYRSAGTVEFVYDSLAGQFYFLEVNTRLQVEHGVTEQV 303 Query: 307 TGIDLVQAMLRIAGGE--PLGLQQSDIRMHGAALEMRINAEDPERNFFPCPGTVAELQWP 364 G+DLV+ M+ +A G+ PL + ++ G A++ R+ AEDP R F PCPG + + +P Sbjct: 304 WGVDLVRWMIELAAGDLPPLAQLAAGLQPRGHAIQARLYAEDPGRQFQPCPGLLTAVDFP 363 Query: 365 --QGEGIRVESHLYAGYRIPPYYDSLLAKLVVHGKDRAQAIARAQAAVLATRITGMATTL 422 G+ +R+++ + AG I PY+D ++AKL+ R A AA+ AT + G+ T Sbjct: 364 PVDGQALRIDTWVEAGCEIAPYFDPMIAKLISWQPTREAARLALDAALRATLLYGVETNQ 423 Query: 423 SLHQWLLADARVQSARFDTGALETWLAERTDT 454 + +LAD S + T LE L R DT Sbjct: 424 HYLRQILADVPFASGQPWTRCLEQ-LVYRADT 454 >gi|300310711|ref|YP_003774803.1| carbamoyl-phosphate synthase large subunit [Herbaspirillum seropedicae SmR1] Length = 1077 Score = 57.8 bits (138), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 62/249 (24%), Positives = 97/249 (38%), Gaps = 36/249 (14%) Query: 98 VNAAGLIFVGPQADTIRRMGDKAEARRTAAAAGVPVVPGSAGELEDIATALACAEEIGYP 157 + A G+ VG D I D+ ++ G+ P E A AL A+EIGYP Sbjct: 649 LEANGVPIVGTSPDMIDAAEDRERFQKLLQDLGLRQPPNRTARTE--ADALQLAQEIGYP 706 Query: 158 LLIKASAGGGGRGIRMARDATELAREFPLAQAEAQAAFGSAAVYLERFIRRARHIEVQIL 217 L+++ S GGR + + + +L R EA + V L+RF+ A ++V L Sbjct: 707 LVVRPSYVLGGRAMEIVHEQRDLERYM----REAVKVSHDSPVLLDRFLNDAIEVDVDCL 762 Query: 218 GDGERA-------------VHLFERECSLQRRRQKVLEEAPSPALTPAQRQALCDSAVRL 264 DGER VH + CSL P +L+ + L + Sbjct: 763 SDGERTFIGGVMEHIEQAGVHSGDSACSL-----------PPYSLSKDTIEELKRQTALM 811 Query: 265 AEQLHYRGAGTLEYLFDDESGE------FFFIEMNTRIQVEHPVTEMITGIDLVQAMLRI 318 A+ L+ G +++ + F +E+N R P TG+ L + R Sbjct: 812 AKGLNVVGLMNVQFAIQQSEVDGKTVDTVFVLEVNPRASRTVPFVSKATGLQLAKIAARC 871 Query: 319 AGGEPLGLQ 327 G+ L Q Sbjct: 872 MVGQSLDSQ 880 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.133 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,207,153 Number of extensions: 45788 Number of successful extensions: 225 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 213 Number of HSP's successfully gapped: 5 Length of query: 462 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 373 Effective length of database: 1,198,455 Effective search space: 447023715 Effective search space used: 447023715 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)