BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0113.p (403 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313395|ref|YP_003777487.1| porin transmembrane protein [He... 794 0.0 gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) ... 148 8e-37 gi|300309527|ref|YP_003773619.1| outer membrane protein (porin) ... 138 1e-33 gi|300311557|ref|YP_003775649.1| outer membrane porin [Herbaspir... 126 3e-30 gi|300313813|ref|YP_003777905.1| transmembrane outer membrane po... 124 1e-29 >gi|300313395|ref|YP_003777487.1| porin transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 403 Score = 794 bits (2051), Expect = 0.0, Method: Compositional matrix adjust. Identities = 402/403 (99%), Positives = 402/403 (99%) Query: 1 MHDKNIARQPSAFKPARLCAALLAAGIALPAWAQSSVTLYGTVDLGLAYSSNQGGASNTY 60 MH KNIARQPSAFKPARLCAALLAAGIALPAWAQSSVTLYGTVDLGLAYSSNQGGASNTY Sbjct: 1 MHAKNIARQPSAFKPARLCAALLAAGIALPAWAQSSVTLYGTVDLGLAYSSNQGGASNTY 60 Query: 61 MNSGALAASKLGLTGTEDLGGGNMALFRLENGFNADTGAQSTAGVLFNRQSYVGLSSKDY 120 MNSGALAASKLGLTGTEDLGGGNMALFRLENGFNADTGAQSTAGVLFNRQSYVGLSSKDY Sbjct: 61 MNSGALAASKLGLTGTEDLGGGNMALFRLENGFNADTGAQSTAGVLFNRQSYVGLSSKDY 120 Query: 121 GQVTVGRQYTPYFQYVGALGPTNVLTGATGAHPGDIDALDTTLRMNNSITYTLPTLGGLQ 180 GQVTVGRQYTPYFQYVGALGPTNVLTGATGAHPGDIDALDTTLRMNNSITYTLPTLGGLQ Sbjct: 121 GQVTVGRQYTPYFQYVGALGPTNVLTGATGAHPGDIDALDTTLRMNNSITYTLPTLGGLQ 180 Query: 181 AGVQYGMGEQAGSAANGSSVSAALRYDYQAFSWSAGYTRLKNLANGNTAGSFGNVGSFAN 240 AGVQYGMGEQAGSAANGSSVSAALRYDYQAFSWSAGYTRLKNLANGNTAGSFGNVGSFAN Sbjct: 181 AGVQYGMGEQAGSAANGSSVSAALRYDYQAFSWSAGYTRLKNLANGNTAGSFGNVGSFAN 240 Query: 241 NSPVNAGYASADSTQLLATAARYTRGDLMLGLNYSNVQYKAGALSAFSQTAVFNTIGLIG 300 NSPVNAGYASADSTQLLATAARYTRGDLMLGLNYSNVQYKAGALSAFSQTAVFNTIGLIG Sbjct: 241 NSPVNAGYASADSTQLLATAARYTRGDLMLGLNYSNVQYKAGALSAFSQTAVFNTIGLIG 300 Query: 301 SYNVSPALVLAAGYSYTAEKARNGISSPAKYNQLSLEEVYSLSKRTALYAIQAYQRASGQ 360 SYNVSPALVLAAGYSYTAEKARNGISSPAKYNQLSLEEVYSLSKRTALYAIQAYQRASGQ Sbjct: 301 SYNVSPALVLAAGYSYTAEKARNGISSPAKYNQLSLEEVYSLSKRTALYAIQAYQRASGQ 360 Query: 361 TLRAGGVGSSIVNAVASVGDSQNGTPSSGSGQFVAMLGIRHSF 403 TLRAGGVGSSIVNAVASVGDSQNGTPSSGSGQFVAMLGIRHSF Sbjct: 361 TLRAGGVGSSIVNAVASVGDSQNGTPSSGSGQFVAMLGIRHSF 403 >gi|300310676|ref|YP_003774768.1| outer membrane protein (porin) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 352 Score = 148 bits (373), Expect = 8e-37, Method: Compositional matrix adjust. Identities = 143/405 (35%), Positives = 181/405 (44%), Gaps = 69/405 (17%) Query: 14 KPARLCAALLAAGIALPAWAQSSVTLYGTVDLGLAY-----SSNQGGASNTYMNSGALAA 68 K L AA+LA G A A AQSSV++YG +DLGL Y S GGA M+SG Sbjct: 2 KKTLLVAAMLAGG-ATTAHAQSSVSIYGLIDLGLTYYSSAPKSGGGGARLLRMDSGIAQG 60 Query: 69 SKLGLTGTEDLGGGNMALFRLENGFNADTGAQSTAGVLFNRQSYVGLSSKDYGQVTVGRQ 128 S++G GTEDLGGG A F LE GF AD G+ G+LF RQS+VGL S +G ++VGRQ Sbjct: 61 SRIGFKGTEDLGGGMNAFFTLETGFGADDGSLGQGGLLFGRQSFVGLGSTQWGSLSVGRQ 120 Query: 129 YTPYF----QY-VGALGPTNVLTGATGAHPGDIDALDTTL----RMNNSITYTLPTLGGL 179 Y QY +GAL P A AL+ + R NNSI Y GG Sbjct: 121 YDFMANIGGQYAMGALSPAGSFGWGLHADAAHGTALNNHIYAGDRTNNSIKYNSAKFGGF 180 Query: 180 QAGVQYGMGEQAGSAANGSSVSAALRYDYQAFSWSAGYTRLKNLANGNTAGSFGNVGSFA 239 G G+GE AG+ G +VSA +YD FS + YT +K ++ GSF Sbjct: 181 SFGAMVGLGETAGNNNAGRTVSATGQYDNGPFSGALAYTDIK-----DSTGSF------- 228 Query: 240 NNSPVNAGYASADSTQLLATAARYTRGDLM-LGLNYSNVQYKAGALSAFSQTAVFNTIGL 298 ST++ ARY G G+ Q K SA + T T+ Sbjct: 229 -------------STRIYGGGARYQIGAFTPFGV---VTQVKNTNTSAKATTYELGTV-- 270 Query: 299 IGSYNVSPALVLAAGYSYTAEKARNGISSPAKYNQLSLEEVYSLSKRTALYAIQAYQRAS 358 Y ++P LAA Y + +K G+ + L Y +SKRT LY Y R Sbjct: 271 ---YALTPLWDLAAAYQF--QKRNQGVGNA---QALVATVDYKISKRTDLYVGAVYDRDK 322 Query: 359 GQTLRAGGVGSSIVNAVASVGDSQNGTPSSGSGQFVAMLGIRHSF 403 G NA G G S+ Q LG+RH F Sbjct: 323 G------------YNAYPVFG---GGIQSATGNQTALRLGMRHRF 352 >gi|300309527|ref|YP_003773619.1| outer membrane protein (porin) signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 390 Score = 138 bits (347), Expect = 1e-33, Method: Compositional matrix adjust. Identities = 133/393 (33%), Positives = 189/393 (48%), Gaps = 48/393 (12%) Query: 35 SSVTLYGTVDLGLAYSSN----QGGASNTY-MNSGALAASKLGLTGTEDLGGGNMALFRL 89 SSVT+YG VD G+ Y S GG + + +NSG + S++G GTEDLGGG A+F L Sbjct: 22 SSVTIYGIVDTGVVYQSKVATPTGGTGSKFSLNSGVIQGSRIGFKGTEDLGGGLSAVFNL 81 Query: 90 ENGFNADTGA---QSTAGVLFNRQSYVGLSSKDYGQVTVGRQYTPYFQYVGALGPTNVLT 146 E GF+ DTGA T+ LF R+S VGL+ ++G V +GRQ T + + + Sbjct: 82 EAGFSNDTGALDDSKTSNTLFRRKSVVGLAG-NFGSVLLGRQ-TDFADTISSYTSVQDFG 139 Query: 147 GATGAHPGDIDALDTTLRMNNSITYTLPTLGGLQAGVQYGMGEQAGSAANGSSVSAALRY 206 G TGA ++D L+ T R NNSI+YT L G + YG GE AG + G + +Y Sbjct: 140 GWTGAAGHNLDRLEGT-RTNNSISYTTANLNGFTGNLMYGFGETAGQTSAGQAFGVGGKY 198 Query: 207 DYQAFSWSAGYTRLKNLANGNTAGSFGNVGSFANNSPVNAGYASADSTQLLATAARYTRG 266 D Y + K G+T + + N N + + ++L AA Y G Sbjct: 199 DNGPLGLGINYYQSK---AGSTPADASLLAATGTNQFTNGTNNGSTAQKVLNVAASYQFG 255 Query: 267 DLMLGLNYSNVQYK-----------AGALSAFSQTAVFNT----IGLIGS-YNVSPALVL 310 + N+S V+ G+L+ S + + I IG+ Y++SP+L L Sbjct: 256 PARVYGNWSRVKQDLNTQANLTAAGVGSLATISNRTLAASNKADIYEIGTAYSLSPSLKL 315 Query: 311 AAGYSYTAEKARNGISSPAKYNQLSLEEVYSLSKRTALYAIQAYQRASGQTLRAGGVGSS 370 A +T + +G+S AK Q+SL Y LSKRT LYA AY +A+ L GV S Sbjct: 316 LAAVDHTRAQF-DGVSGKAKLTQVSLGTDYWLSKRTDLYAFLAYSKAT--DLNNPGVADS 372 Query: 371 IVNAVASVGDSQNGTPSSGSGQFVAMLGIRHSF 403 +GT + Q +GIRH F Sbjct: 373 ------------SGTDGN---QGAVAVGIRHKF 390 >gi|300311557|ref|YP_003775649.1| outer membrane porin [Herbaspirillum seropedicae SmR1] Length = 374 Score = 126 bits (316), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 127/383 (33%), Positives = 177/383 (46%), Gaps = 37/383 (9%) Query: 31 AWAQSSVTLYGTVDLGLAYSSNQG--GASNTYMNSGALAASKLGLTGTEDLGGGNMALFR 88 A+AQS+VT+YG VD + Y SN G SN M++GA+A S++G GTEDLGGG A+F+ Sbjct: 19 AFAQSNVTIYGIVDTSIHYLSNANAQGNSNVRMDNGAIANSRIGFKGTEDLGGGLKAMFQ 78 Query: 89 LENGFNADTGAQSTAGVLFNRQSYVGLSSKDYGQVTVGRQYTPYFQYVGALGPTNVLTGA 148 LENGF++DTGA G LF RQS+VGL +G+V VGRQ TP F + + LT Sbjct: 79 LENGFSSDTGAMQQGGSLFGRQSWVGLQGG-FGKVRVGRQNTPLFDLLAD--HFDPLTVG 135 Query: 149 TGAHPGDIDALDTTLRMNNSITYTLPTLGGLQAGVQYGMGEQAGSAANGSSVSAALRYDY 208 A + A + +R N + Y G + A + GE+AGS GS S+ALRY Sbjct: 136 NYASNSWLPAAASLVRSPNMLRYD-GDFGPVAASASWAFGEKAGSVRTGSQFSSALRYTA 194 Query: 209 QAFSWSAGYTRLKNLANGNTAGSFGNVGSFAN--NSPVNAGYASADSTQLLATAARYTRG 266 + GY + + N + + N+ + + + V G+ + L + G Sbjct: 195 GPLALGGGYQQTVSSTNSSLKHTTWNLSASYDFGVAKVFGGWLNIKDDSGLTGSVMAADG 254 Query: 267 DLMLGLNYSNVQYKAGALSAFSQTAVFNTIGLI--GSYNVSPALVLAAGYSYTAEKARNG 324 D+ SN AGA + S G SY + AL L + Y K + Sbjct: 255 DV------SNGWTTAGAANNGSTGVKRKDNGFFLGSSYKATNALTLTGAFYYDRSKNVSY 308 Query: 325 ISS----PAKYNQLSLEEVYSLSKRTALYAIQAYQRASGQTLRAGGVGSSIVNAVASVGD 380 +S+ K L Y+LSKRT LY Y +A AV + + Sbjct: 309 VSTGNVGDGKRYTLVGWADYALSKRTQLYTTIDYNKAKDA-------------AVYELTN 355 Query: 381 SQNGTPSSGSGQFVAMLGIRHSF 403 S NG S +GIRH F Sbjct: 356 SANGKNS----LTTVGVGIRHIF 374 >gi|300313813|ref|YP_003777905.1| transmembrane outer membrane porin signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 354 Score = 124 bits (311), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 131/383 (34%), Positives = 164/383 (42%), Gaps = 61/383 (15%) Query: 33 AQSSVTLYGTVDLGLAYSSN---QGGASNTYMNSGALAASKLGLTGTEDLGGGNMALFRL 89 AQSSVT+YG VD G+ Y +N G S T M SGA S++G GTEDLGGG ALF+L Sbjct: 21 AQSSVTIYGLVDTGVEYLNNAGPNGAGSVTRMTSGATNTSRIGFRGTEDLGGGLKALFQL 80 Query: 90 ENGFNADTGA-QSTAGVLFNRQSYVGLSSKDYGQVTVGRQYTPYFQYVGALGPTN----- 143 ENGF D G+ A LFNRQS VGL +G+V GR Y+ + ++G P Sbjct: 81 ENGFKLDNGSFDGDANQLFNRQSTVGLEG-GFGRVVAGRSYSTTYDFLGPFDPMGYSANY 139 Query: 144 ---VLTGATGAHPGDIDALDTTLRMNNSITYTLPTLGGLQAGVQYGMGEQAGSAANGSSV 200 GATG D + T + +N + Y G++ G YG GE AG NG+ Sbjct: 140 SWATTAGATGNRK---DGMPTGV--SNLVKYQ-GEFSGVKIGAMYGFGEVAGETRNGAKY 193 Query: 201 SAALRYDYQAFSWSAGYTRLKNLANGNTAGSFGNVGSFANNSPVNAGYASADSTQLLATA 260 L + FS A Y R N + A N+P Y A S L A Sbjct: 194 GVGLGWGTGPFSVVATYDR--------------NNSATATNTP----YDRATSAHLAAGY 235 Query: 261 ARYTRGDLMLGLNYSNVQYKAGALSAFSQTAVFNTIGLIGSYNVSPALVLAAGYSYTAEK 320 LG Y GA S F G +Y +PA+ L Y K Sbjct: 236 QPSEAWKFNLGYRYYKKSLATGAADLRSD---FFWGG--AAYKFTPAMTLIGAIYYQNIK 290 Query: 321 ARNGISSPAKYNQLSLEEVYSLSKRTALYAIQAYQRASGQTLRAGGVGSSIVNAVASVGD 380 + P Y SL Y++SKRT LY AY +A L VG S +A Sbjct: 291 NVANDADPIMY---SLRLKYAMSKRTDLYLSTAYAKAKNGQL----VGLSRDDA------ 337 Query: 381 SQNGTPSSGSGQFVAMLGIRHSF 403 GS QF LG++H F Sbjct: 338 ------GYGSSQFGMTLGMQHRF 354 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.313 0.128 0.361 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,143,102 Number of extensions: 45264 Number of successful extensions: 188 Number of sequences better than 1.0e-08: 14 Number of HSP's gapped: 148 Number of HSP's successfully gapped: 14 Length of query: 403 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 316 Effective length of database: 1,207,925 Effective search space: 381704300 Effective search space used: 381704300 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 132 (55.5 bits)