BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0111.p (428 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313393|ref|YP_003777485.1| major facilitator superfamily p... 847 0.0 gi|300313884|ref|YP_003777976.1| phthalate permease of the major... 456 e-129 gi|300312451|ref|YP_003776543.1| D-galactonate transporter prote... 144 2e-35 gi|300309918|ref|YP_003774010.1| major facilitator superfamily p... 124 1e-29 gi|300310490|ref|YP_003774582.1| major facilitator superfamily p... 87 2e-18 >gi|300313393|ref|YP_003777485.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 847 bits (2188), Expect = 0.0, Method: Compositional matrix adjust. Identities = 427/428 (99%), Positives = 428/428 (100%) Query: 1 LKTIKGLRWWIIVLVCLGTITNYLARNSLGVLAPTLKDELGMSTQQYSYVVAAFQVGYTI 60 +KTIKGLRWWIIVLVCLGTITNYLARNSLGVLAPTLKDELGMSTQQYSYVVAAFQVGYTI Sbjct: 1 MKTIKGLRWWIIVLVCLGTITNYLARNSLGVLAPTLKDELGMSTQQYSYVVAAFQVGYTI 60 Query: 61 MQPVCGFIVDLIGLRIGFALFGVLWSVAGVLHAGATGWLSLAGFRALMGLTEAAAIPSGM 120 MQPVCGFIVDLIGLRIGFALFGVLWSVAGVLHAGATGWLSLAGFRALMGLTEAAAIPSGM Sbjct: 61 MQPVCGFIVDLIGLRIGFALFGVLWSVAGVLHAGATGWLSLAGFRALMGLTEAAAIPSGM 120 Query: 121 KAVAEWFPDKEKSVAVGWFNSGTSLGAMLAPPLVIFLQIRYGWQSAFVVTGILGFVWAAL 180 KAVAEWFPDKEKSVAVGWFNSGTSLGAMLAPPLVIFLQIRYGWQSAFVVTGILGFVWAAL Sbjct: 121 KAVAEWFPDKEKSVAVGWFNSGTSLGAMLAPPLVIFLQIRYGWQSAFVVTGILGFVWAAL 180 Query: 181 WFAFYRSPRDHAALSDSERETIISGQLRPVSAVRGKRPVKDVVRSRRFWAIALARFFAEP 240 WFAFYRSPRDHAALSDSERETIISGQLRPVSAVRGKRPVKDVVRSRRFWAIALARFFAEP Sbjct: 181 WFAFYRSPRDHAALSDSERETIISGQLRPVSAVRGKRPVKDVVRSRRFWAIALARFFAEP 240 Query: 241 AWQTFSFWIPLYFVNERHLDLKSIAMFAWLPFLAADLGGLAGGYLSPFLINRFRIPLVWS 300 AWQTFSFWIPLYFVNERHLDLKSIAMFAWLPFLAADLGGLAGGYLSPFLINRFRIPLVWS Sbjct: 241 AWQTFSFWIPLYFVNERHLDLKSIAMFAWLPFLAADLGGLAGGYLSPFLINRFRIPLVWS 300 Query: 301 RVCGVVLGAVMMIGPACVGLVASPYVAIALFCVGGFAHQMISGLVNTLSADVFDPEEVGT 360 RVCGVVLGAVMMIGPACVGLVASPYVAIALFCVGGFAHQMISGLVNTLSADVFDPEEVGT Sbjct: 301 RVCGVVLGAVMMIGPACVGLVASPYVAIALFCVGGFAHQMISGLVNTLSADVFDPEEVGT 360 Query: 361 ASGFAGMSAWIGGLGFSLLVGALADTVGYGPLFGMLGAFDLIGAVLLIILIRGQSTEERA 420 ASGFAGMSAWIGGLGFSLLVGALADTVGYGPLFGMLGAFDLIGAVLLIILIRGQSTEERA Sbjct: 361 ASGFAGMSAWIGGLGFSLLVGALADTVGYGPLFGMLGAFDLIGAVLLIILIRGQSTEERA 420 Query: 421 ARSLQSSV 428 ARSLQSSV Sbjct: 421 ARSLQSSV 428 >gi|300313884|ref|YP_003777976.1| phthalate permease of the major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 418 Score = 456 bits (1174), Expect = e-129, Method: Compositional matrix adjust. Identities = 219/401 (54%), Positives = 289/401 (72%), Gaps = 1/401 (0%) Query: 1 LKTIKGLRWWIIVLVCLGTITNYLARNSLGVLAPTLKDELGMSTQQYSYVVAAFQVGYTI 60 +K ++G+RW+++ LV L I NYLARN+L V APT+ EL MSTQQYSY+V A+Q+ Y + Sbjct: 1 MKYVRGIRWYMVTLVTLALIVNYLARNTLSVAAPTMMKELDMSTQQYSYIVVAWQICYAV 60 Query: 61 MQPVCGFIVDLIGLRIGFALFGVLWSVAGVLHAGATGWLSLAGFRALMGLTEAAAIPSGM 120 MQPV G+I+D +G +IGF +F + WS+ A ATGW SLA FRAL+G+TEAA IP G+ Sbjct: 61 MQPVAGYILDAVGTKIGFGIFALAWSLVCAAAAFATGWQSLAFFRALLGITEAAGIPGGV 120 Query: 121 KAVAEWFPDKEKSVAVGWFNSGTSLGAMLAPPLVIFLQIRYGWQSAFVVTGILGFVWAAL 180 KA EWFP KE+SVA+GWFN G+S+GA+ APPLV++ + GW+ +FVV G LG +W L Sbjct: 121 KASTEWFPAKERSVAIGWFNIGSSIGALCAPPLVVWTILHGGWKMSFVVVGALGVIWFVL 180 Query: 181 WFAFYRSPRDHAALSDSERETIISGQLRPVSAVRGKRPVKDVVRSRRFWAIALARFFAEP 240 W FY+SPRD LS ER I+ GQ + V+ + K +VRSR FW+IA+ RF +EP Sbjct: 181 WMLFYKSPRDQKLLSPEERAYILEGQEKSPEKVQRESWTK-IVRSRNFWSIAIPRFLSEP 239 Query: 241 AWQTFSFWIPLYFVNERHLDLKSIAMFAWLPFLAADLGGLAGGYLSPFLINRFRIPLVWS 300 AWQTF+ WIPLY ERH+++K IAMFAWLPFLAAD+G + GGYLSP ++ L S Sbjct: 240 AWQTFNAWIPLYMATERHMNIKEIAMFAWLPFLAADIGCVLGGYLSPLFHKHLKVSLFTS 299 Query: 301 RVCGVVLGAVMMIGPACVGLVASPYVAIALFCVGGFAHQMISGLVNTLSADVFDPEEVGT 360 R +++G++ MIGPACVG V SPYVAIAL +GGFAHQ +SG + ++++DVF +V T Sbjct: 300 RKLVMLVGSLSMIGPACVGFVDSPYVAIALLSIGGFAHQTLSGALYSITSDVFTKNQVAT 359 Query: 361 ASGFAGMSAWIGGLGFSLLVGALADTVGYGPLFGMLGAFDL 401 A+G GMS ++G F+LL G L +GYGPLF +L AFDL Sbjct: 360 ATGLTGMSGYLGATLFTLLFGILVTQIGYGPLFVLLAAFDL 400 >gi|300312451|ref|YP_003776543.1| D-galactonate transporter protein [Herbaspirillum seropedicae SmR1] Length = 439 Score = 144 bits (362), Expect = 2e-35, Method: Compositional matrix adjust. Identities = 105/397 (26%), Positives = 184/397 (46%), Gaps = 18/397 (4%) Query: 7 LRWWIIVLVCLGTITNYLARNSLGVLAPTLKDELGMSTQQYSYVVAAFQVGYTIMQPVCG 66 +R+ I+ L+ + T NY R +L + +K EL + Y+ +AF Y + Q G Sbjct: 11 VRYLILFLLFVVTTVNYADRATLSIAGSAMKTELSLDPVMMGYIFSAFSWAYVLAQLPGG 70 Query: 67 FIVDLIGLRIGFALFGVLWSVAGVLHAGATGWLSLAG-------FRALMGLTEAAAIPSG 119 +++D G + + LWSV L AG T W+S A R ++GL EA + P+ Sbjct: 71 WLLDRFGSKKIYMWSIALWSVFTFLQAGVT-WMSTAAAVASLFLLRFMVGLAEAPSFPAN 129 Query: 120 MKAVAEWFPDKEKSVAVGWFNSGTSLGAMLAPPLVIFLQIRYGWQSAFVVTGILGFVWAA 179 + VA WFP KE+ A FNS A+L PL+ ++ Y W F V G++G A Sbjct: 130 GRIVAAWFPTKERGTASAIFNSAQYFAAVLFTPLMAWIVHTYSWHHVFTVMGVVGIALAL 189 Query: 180 LWFAFYRSPRDHAALSDSERETIISG------QLRPVSA--VRGKRPVKDVVRSRRFWAI 231 +W SP+DH ++ +E I SG R V+A +G+ ++ ++ +R + Sbjct: 190 IWGRIIYSPKDHPRINQAELAHIESGGGLVDMDNRKVAAPSTKGRGYIRQLLSNRMLLGV 249 Query: 232 ALARFFAEPAWQTFSFWIPLYFVNERHLDLKSIAMFAWLPFLAADLGGLAGGYLSPFLIN 291 + ++ F W P+Y V ER + + A +P + +GG+ GG +S LI Sbjct: 250 YIGQYCINVITYFFLTWFPVYLVQERGMSILKAGFVASIPAVCGFIGGVVGGLISDRLIK 309 Query: 292 RFRIPLVWSRVCGVVLGAVMMIGPACVGLVASPYVAIALFCVGGFAHQMISGLVNTLSAD 351 R + + W+R +V G ++ + V + ++ + + + F + + L + AD Sbjct: 310 R-GLSVTWARKIPIVGGMLLSMTMILCNYVEAQWMVVGIMALAFFG-KGVGALGWAVVAD 367 Query: 352 VFDPEEVGTASGFAGMSAWIGGLGFSLLVGALADTVG 388 + VG + G M + G+ +++G + G Sbjct: 368 TAPKQIVGLSGGLFNMFGNVAGITTPIVIGYIVKETG 404 >gi|300309918|ref|YP_003774010.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 124 bits (311), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 86/308 (27%), Positives = 143/308 (46%), Gaps = 7/308 (2%) Query: 15 VCLGTITNYLARNSLGVLAPTLKDELGMSTQQYSYVVAAFQVGYTIMQPVCGFIVDLIGL 74 + L I NYL R++L + ++ EL +S Q +++AF + Y Q G ++D G Sbjct: 22 LMLAGIVNYLDRSTLSIANHSVSQELNLSASQMGLLLSAFSLAYAFSQLPIGAMLDKFGA 81 Query: 75 RIGFALFGVLWSVAGVLHAGATGWLSLAGFRALMGLTEAAAIPSGMKAVAEWFPDKEKSV 134 R+ +WSVA + R +GL EA P+G K V+EWF +E+ Sbjct: 82 RVMLGAGMFIWSVAQLAGGFVNNLQQFLVARVFLGLGEAPQFPAGAKVVSEWFAMRERGG 141 Query: 135 AVGWFNSGTSLGAMLAPPLVIFLQIRYGWQSAFVVTGILGFVWAALWFAFYRSPRDHAAL 194 G F + +++G LAPP++ L + +GW+ FV+ G+LG + W+ FYR+ R+ L Sbjct: 142 PTGIFVASSTIGPALAPPILTILMLSFGWRMMFVIMGVLGIAVSVGWYLFYRN-RNEVQL 200 Query: 195 SDSERETIISGQLRP--VSAVRGKRPVKDVVRSRRFWAIALARFFAEPAWQTFSFWIPLY 252 + SE + +A K + R W + + W+P Y Sbjct: 201 TPSEVLYLADDHDNDPNANAKLSWSEWKSLFTYRTTWGMLFGFMGVIYMVWLYLTWLPAY 260 Query: 253 FVNERHLDLKSIAMFAWLPFLAADLGGLAGGYLSPFLINRFRIPLVWSR---VCGVVLGA 309 +ERHL + +P++ LG L+ GY++ L+ R +P + SR +C ++GA Sbjct: 261 LEHERHLSIARTGWVVVIPYIFGTLGMLSSGYIADGLMKR-GMPAIRSRKWPICAGLIGA 319 Query: 310 VMMIGPAC 317 PA Sbjct: 320 GAFTIPAA 327 >gi|300310490|ref|YP_003774582.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 447 Score = 87.4 bits (215), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 102/427 (23%), Positives = 169/427 (39%), Gaps = 36/427 (8%) Query: 2 KTIKGLRWWIIVLVCLGTITNYLARNSLGVLAPTLKDELGMSTQQYSYVVAAFQVGYTIM 61 + ++W I+ L I NY+ R ++G + L +LG+ + F VGY + Sbjct: 16 SAVSKIKWRILPLFVAMFIINYIDRVNIGFVRSHLSTDLGIGAAAFGLGAGLFFVGYALF 75 Query: 62 QPVCGFIVDLIGLRIGFALFGVLWSVAGVLHAGATGWLSLAGFRALMGLTEAAAIPSGMK 121 + ++ G R W + A S R L+G+ EA P + Sbjct: 76 EVPSNMLLQRYGARAWLTRIMFTWGLVAACMAFVQSETSFYFMRFLLGVAEAGFFPGVVY 135 Query: 122 AVAEWFPDKEKSVAVGWFNSGTSLGAMLAPPLV-IFLQIR----YGWQSAFVVTGILGFV 176 +W P+ E+ A+ F SG+++ ++L+ P+ L I +GWQ F++ G V Sbjct: 136 YFTQWLPNHERGRAMAVFLSGSAIASILSGPITGALLLIEGGGLHGWQWMFIIEGGASMV 195 Query: 177 WA-ALWFAFYRSPRDHAALSDSERE---TIISGQL------RP--VSAVRGKRPVKDVVR 224 + +W +PR+ LS +RE +I+ + RP VSA+ R + V+ Sbjct: 196 FCVVVWLVLDSTPREAKWLSQEQREALAAVIAAEQEQRAANRPAHVSALSLLRDPQIVLF 255 Query: 225 SRRFWAIALARFFAEPAWQTFSFWIPLYFVNERHLDLKSIAMFAWLPFLAADLGGLAGGY 284 ++AI L + A +FW+P H + + +F +P++ A +A Y Sbjct: 256 CFIYFAIQLTIYGA-------TFWLPTIIKKMGHFNDFQVGLFNSIPWIVA----IAAMY 304 Query: 285 LSPFLINRFRIPLVWSRVCGVVLGAVMMIGPACVGLVASPYVAIALFCVGGFAHQMISGL 344 L L R++ P W V+ A M I VA P + C G + S L Sbjct: 305 LFAVLAARYKKPQFWVATTLVISAAGMAISA-----VAGPVIGFVAVCFAGIGFKAASSL 359 Query: 345 VNTLSADVFDPEEVGTASGFAGMSAWIGGLGFSLLVGALADTVG--YGPLFGMLGAFDLI 402 + D +GG G L G G LF + GA L+ Sbjct: 360 FWPIPQAYLDARIAAAVLALINSLGNLGGFFAPATFGYLEQKTGSIQGGLFALSGA-SLL 418 Query: 403 GAVLLII 409 AVL+ + Sbjct: 419 AAVLVFL 425 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.141 0.452 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,393,543 Number of extensions: 60895 Number of successful extensions: 392 Number of sequences better than 1.0e-08: 24 Number of HSP's gapped: 370 Number of HSP's successfully gapped: 24 Length of query: 428 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 340 Effective length of database: 1,203,190 Effective search space: 409084600 Effective search space used: 409084600 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)