BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0100.p (303 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313359|ref|YP_003777451.1| multidrug ABC transporter ATPas... 617 e-178 gi|300310523|ref|YP_003774615.1| branched-chain amino acid ABC t... 120 2e-28 gi|300309521|ref|YP_003773613.1| branched-chain amino acid ABC t... 120 2e-28 gi|300311269|ref|YP_003775361.1| multidrug ABC transporter ATPas... 119 3e-28 gi|300310327|ref|YP_003774419.1| metal ion ABC transporter ATPas... 116 2e-27 >gi|300313359|ref|YP_003777451.1| multidrug ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 303 Score = 617 bits (1590), Expect = e-178, Method: Compositional matrix adjust. Identities = 302/303 (99%), Positives = 303/303 (100%) Query: 1 MAALQINDIKKRYQSLQALGGVSLAIEEGEFFGLLGPNGAGKTTLISIIAGLNRADSGTV 60 MAALQINDIKKRYQSLQALGGVSLAIEEGEFFGLLGPNGAGKTTLISIIAGLNRADSGTV Sbjct: 1 MAALQINDIKKRYQSLQALGGVSLAIEEGEFFGLLGPNGAGKTTLISIIAGLNRADSGTV 60 Query: 61 TIHGHDVVSDYRAARMKLGVVPQELVFDPFFTVRETLRMQSGYFGLSSNDKWIDEVMENL 120 TIHGHDVVSDYRAARMKLGVVPQELVFDPFFTVRETLRMQSGYFGLSSNDKWIDEVMENL Sbjct: 61 TIHGHDVVSDYRAARMKLGVVPQELVFDPFFTVRETLRMQSGYFGLSSNDKWIDEVMENL 120 Query: 121 DLTNKADTNMRALSGGMKRRVLVAQALVHKPPVIVLDEPTAGVDVELRQTLWQFIGRLNR 180 DLTNKADTNMRALSGGMKRRVLVAQALVHKPPVIVLDEPTAGVDVELRQTLWQFIGRLNR Sbjct: 121 DLTNKADTNMRALSGGMKRRVLVAQALVHKPPVIVLDEPTAGVDVELRQTLWQFIGRLNR 180 Query: 181 EGHTIVLTTHYLEEAQALCNRIAMLKFGKVVALDSTEGLIRRISGSQMLLRLKRGSLPEG 240 EGHTIVLTTHYLEEAQALCNRIAMLKFGKVVALDSTEGLIRRISGSQM+LRLKRGSLPEG Sbjct: 181 EGHTIVLTTHYLEEAQALCNRIAMLKFGKVVALDSTEGLIRRISGSQMVLRLKRGSLPEG 240 Query: 241 LKSLVTHPEELLSGNKYTLRVNDYQEVEPILAALRAAGAEIDDLQLQQADLEDVFIQIMG 300 LKSLVTHPEELLSGNKYTLRVNDYQEVEPILAALRAAGAEIDDLQLQQADLEDVFIQIMG Sbjct: 241 LKSLVTHPEELLSGNKYTLRVNDYQEVEPILAALRAAGAEIDDLQLQQADLEDVFIQIMG 300 Query: 301 DEK 303 DEK Sbjct: 301 DEK 303 >gi|300310523|ref|YP_003774615.1| branched-chain amino acid ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 255 Score = 120 bits (300), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 75/228 (32%), Positives = 119/228 (52%), Gaps = 19/228 (8%) Query: 4 LQINDIKKRYQSLQALGGVSLAIEEGEFFGLLGPNGAGKTTLISIIAGLNRADSGTVTIH 63 L+I D+ KR+ LQAL GV + IE G+ +GL+GPNGAGKTT ++I GL + D+GT + Sbjct: 6 LKIRDVSKRFGGLQALNGVGITIERGQIYGLIGPNGAGKTTFFNVITGLYQPDTGTFELD 65 Query: 64 G--HDVVSDYRAARMKLGVVPQELVFDPFFTVRET------LRMQSGYFGLSSNDKWIDE 115 G + + + A+ + Q + TV E +R + FG K E Sbjct: 66 GKPYSPSAPHEVAKAGIARTFQNIRLFGEMTVLENVMVGCHVRTKQNVFGAVFRHKAARE 125 Query: 116 -----------VMENLDLTNKADTNMRALSGGMKRRVLVAQALVHKPPVIVLDEPTAGVD 164 +++ + + A R LS G +RR+ +A+AL P ++ LDEP AG++ Sbjct: 126 EEAAIREKSQKLLDFVGIGQFAKRTARHLSYGDQRRLEIARALATDPQLLALDEPAAGMN 185 Query: 165 VELRQTLWQFIGRLNREGHTIVLTTHYLEEAQALCNRIAMLKFGKVVA 212 + L + + ++ EG TI+L H ++ LCNRI +L +GK +A Sbjct: 186 ATEKLGLRELLVKIQAEGKTILLIEHDVKLMMGLCNRITVLDYGKPIA 233 >gi|300309521|ref|YP_003773613.1| branched-chain amino acid ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 255 Score = 120 bits (300), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 75/228 (32%), Positives = 119/228 (52%), Gaps = 19/228 (8%) Query: 4 LQINDIKKRYQSLQALGGVSLAIEEGEFFGLLGPNGAGKTTLISIIAGLNRADSGTVTIH 63 L+I D+ KR+ LQAL GV + IE G+ +GL+GPNGAGKTT ++I GL + D+GT + Sbjct: 6 LKIRDVSKRFGGLQALNGVGITIERGQIYGLIGPNGAGKTTFFNVITGLYQPDTGTFELD 65 Query: 64 G--HDVVSDYRAARMKLGVVPQELVFDPFFTVRET------LRMQSGYFGLSSNDKWIDE 115 G + + + A+ + Q + TV E +R + FG K E Sbjct: 66 GKPYSPSAPHEVAKAGIARTFQNIRLFGEMTVLENVMVGCHVRTRQNVFGAVFRHKAARE 125 Query: 116 -----------VMENLDLTNKADTNMRALSGGMKRRVLVAQALVHKPPVIVLDEPTAGVD 164 +++ + + A R LS G +RR+ +A+AL P ++ LDEP AG++ Sbjct: 126 EEAAIREKSQKLLDFVGIGQFAKRTARHLSYGDQRRLEIARALATDPQLLALDEPAAGMN 185 Query: 165 VELRQTLWQFIGRLNREGHTIVLTTHYLEEAQALCNRIAMLKFGKVVA 212 + L + + ++ EG TI+L H ++ LCNRI +L +GK +A Sbjct: 186 ATEKLGLRELLVKIQAEGKTILLIEHDVKLMMGLCNRITVLDYGKPIA 233 >gi|300311269|ref|YP_003775361.1| multidrug ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 913 Score = 119 bits (297), Expect = 3e-28, Method: Compositional matrix adjust. Identities = 76/236 (32%), Positives = 128/236 (54%), Gaps = 9/236 (3%) Query: 1 MAALQINDIKKRYQSLQALGGVSLAIEEGEFFGLLGPNGAGKTTLISIIAGLNRADSGTV 60 + A +++D+ Y AL V L I G GL+GP+G GK+TL+S+IAG R +G + Sbjct: 5 ICAARVHDVSHSYGKNPALSHVELDIPGGCMAGLIGPDGVGKSTLLSLIAGARRIRAGRI 64 Query: 61 TIHGHDV--VSDYRAARMKLGVVPQELVFD--PFFTVRETLRMQSGYFGLSSNDK--WID 114 + G D+ + A + +PQ L + P +V E L+ + FG + ++ ID Sbjct: 65 DVLGGDIGQAAHRDAVGPCIAYMPQGLGKNLYPTLSVEENLQFIARLFGQDAQERRQRID 124 Query: 115 EVMENLDLTNKADTNMRALSGGMKRRVLVAQALVHKPPVIVLDEPTAGVDVELRQTLWQF 174 ++ + L LSGGMK+++ + AL+H P +++LDEPT GVD R W+ Sbjct: 125 DLTHSTGLQPFLARPAGQLSGGMKQKLSLCCALIHDPDLLILDEPTTGVDPLARAQFWEL 184 Query: 175 IGRLNRE--GHTIVLTTHYLEEAQALCNRIAMLKFGKVVALDSTEGLIRRISGSQM 228 I RL + G ++++ T Y++EAQA + +A++ G+V+A + L+ R S + Sbjct: 185 IARLRQRHPGMSVIVATAYMDEAQAF-DWLAVMDQGQVLATGTPAALLARTGASSL 239 Score = 114 bits (285), Expect = 9e-27, Method: Compositional matrix adjust. Identities = 70/224 (31%), Positives = 116/224 (51%), Gaps = 5/224 (2%) Query: 3 ALQINDIKKRY-QSLQALGGVSLAIEEGEFFGLLGPNGAGKTTLISIIAGLNRADSGTVT 61 A++ ++ R+ A+ VS I GE FG LG NG GKTT + ++ GL A SG Sbjct: 271 AIEAENLSMRFADGFTAVDHVSFRIRRGEIFGFLGSNGCGKTTTMKMLTGLLPASSGRAR 330 Query: 62 IHGHDVVSDYRAARMKLGVVPQELVFDPFFTVRETLRMQSGYFGLSSND--KWIDEVMEN 119 + G ++ R ++G + Q +VR+ L + + F L + + E+ Sbjct: 331 LFGQEIDPRDVCLRKRVGYMSQSFSLYKDLSVRQNLALHARLFHLPAAQIGPRVAEMARR 390 Query: 120 LDLTNKADTNMRALSGGMKRRVLVAQALVHKPPVIVLDEPTAGVDVELRQTLWQFIGRLN 179 L D+ L G+++R+ +A A +H P +++LDEPT+GVD R WQ + L+ Sbjct: 391 FALMPVMDSLAADLPMGIRQRLSLAVATIHGPEILILDEPTSGVDPVARDHFWQLLVTLS 450 Query: 180 REGH-TIVLTTHYLEEAQALCNRIAMLKFGKVVALDSTEGLIRR 222 R TI L+TH++ EA C+R++M+ GKV+ D+ L+ + Sbjct: 451 RRDKVTIFLSTHFMNEALR-CDRMSMMHAGKVLDSDTPAALMHK 493 >gi|300310327|ref|YP_003774419.1| metal ion ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 345 Score = 116 bits (291), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 68/215 (31%), Positives = 128/215 (59%), Gaps = 7/215 (3%) Query: 4 LQINDIKKRYQSLQALGGVSLAIEEGEFFGLLGPNGAGKTTLISIIAGLNRADSGTVTIH 63 ++I + +R+ +++A+ V L+I +GE FG++G +GAGK+TL+ + LNR SG + + Sbjct: 2 IEIQAVTQRFGNVEAVRKVDLSIRKGEIFGIIGRSGAGKSTLVRTLNLLNRPTSGRIVLD 61 Query: 64 GHDVVS----DYRAARMKLGVVPQELVFDPFFTVRETLRMQSGYFGLSSND--KWIDEVM 117 G D+ S R AR +G++ Q +V + + + G S + ++ ++ Sbjct: 62 GQDLTSLSAAQLREARRGIGMIFQHFNLLSSRSVYDNIALPLELAGKSKAEIAAKVEPLL 121 Query: 118 ENLDLTNKADTNMRALSGGMKRRVLVAQALVHKPPVIVLDEPTAGVDVELRQTLWQFIGR 177 E + LT D +SGG K+RV +A+AL + P V++ DE T+ +D E +++ + + + Sbjct: 122 ELVGLTALRDRYPAQISGGQKQRVGIARALANDPKVLLSDEATSALDPETTRSILELLRK 181 Query: 178 LNRE-GHTIVLTTHYLEEAQALCNRIAMLKFGKVV 211 +N+E G TIVL TH +E + +C+R+A+++ G+V+ Sbjct: 182 INKELGLTIVLITHQMEVIKQICDRVAVMEAGQVI 216 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.137 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 897,851 Number of extensions: 37115 Number of successful extensions: 497 Number of sequences better than 1.0e-08: 130 Number of HSP's gapped: 325 Number of HSP's successfully gapped: 148 Length of query: 303 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 218 Effective length of database: 1,217,395 Effective search space: 265392110 Effective search space used: 265392110 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)