BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0093.p (372 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313353|ref|YP_003777445.1| histidinol-phosphate aminotrans... 708 0.0 gi|300313983|ref|YP_003778075.1| histidinol-phosphate aminotrans... 145 4e-36 gi|300313928|ref|YP_003778020.1| GntR family transcription regul... 64 2e-11 gi|300311875|ref|YP_003775967.1| aspartate/tyrosine/aromatic ami... 62 9e-11 gi|300311954|ref|YP_003776046.1| cobalamin biosynthesis protein ... 55 6e-09 >gi|300313353|ref|YP_003777445.1| histidinol-phosphate aminotransferase 1 protein [Herbaspirillum seropedicae SmR1] Length = 372 Score = 708 bits (1828), Expect = 0.0, Method: Compositional matrix adjust. Identities = 364/372 (97%), Positives = 367/372 (98%) Query: 1 MKPSVPKPEPESIDQLIANVIRPVVRGWQSYHVPSAEGMVKLDAMENPYLLPAELREQLA 60 MKPSVPKPEPESIDQLIANVIRPVVRGWQSYHVPSAEGMVKLDAMENPYLLPAELREQLA Sbjct: 1 MKPSVPKPEPESIDQLIANVIRPVVRGWQSYHVPSAEGMVKLDAMENPYLLPAELREQLA 60 Query: 61 QRLAALELNRYPVPAYTALKAAICKRLGVPAGYDVLLGNGSDELIALLSVACAQPGRTIL 120 QRLAALELNRYPVP+YTALKAAICK LGVPAGYDVLLGNGSDELI LLSVACAQPGRTIL Sbjct: 61 QRLAALELNRYPVPSYTALKAAICKGLGVPAGYDVLLGNGSDELITLLSVACAQPGRTIL 120 Query: 121 APEPGFVMYGVSAKMAGLEYVGVPLRADLSLDLPAMLAAMEAHKPVITWLGYPNNPTGTL 180 APEPGFVMYGVSAKMAGLEYVGVPL ADLSLDLPAMLAAMEAHKPVITWLGYPNNPTGTL Sbjct: 121 APEPGFVMYGVSAKMAGLEYVGVPLCADLSLDLPAMLAAMEAHKPVITWLGYPNNPTGTL 180 Query: 181 YEMADVLRIVEAAAPYGLVVVDEAYQPFAASTLMPALPQHNNLVLMRTVSKLGLAGIRLG 240 YEMADVLRIVEAAAPYGLVVVDEAYQPFAASTLMPALPQHNNLVLMRTVSKLGLAGIRLG Sbjct: 181 YEMADVLRIVEAAAPYGLVVVDEAYQPFAASTLMPALPQHNNLVLMRTVSKLGLAGIRLG 240 Query: 241 YLSAAPALLRELDKVRPPYNVNVLTEAAALFVLEHLDVLQAQAARLRQARSALPAELAAL 300 YLSAAPALLRELDKVRPPYNVNVLTEAAALF+LEHLDVLQAQAARLRQARSAL AELAAL Sbjct: 241 YLSAAPALLRELDKVRPPYNVNVLTEAAALFMLEHLDVLQAQAARLRQARSALAAELAAL 300 Query: 301 DGVQVFPSAANFLLIRVPGADKVCASLLAHRVLVKNAGRMHVLLQNCLRITVSSDEENAM 360 DGVQVFPSAANFLLIRVP ADKVCASLLAHRVLVKNAGRMHVLLQNCLRITVSSD+ENAM Sbjct: 301 DGVQVFPSAANFLLIRVPDADKVCASLLAHRVLVKNAGRMHVLLQNCLRITVSSDKENAM 360 Query: 361 FLAALKTALREL 372 FLAALKTALREL Sbjct: 361 FLAALKTALREL 372 >gi|300313983|ref|YP_003778075.1| histidinol-phosphate aminotransferase 1 protein [Herbaspirillum seropedicae SmR1] Length = 360 Score = 145 bits (367), Expect = 4e-36, Method: Compositional matrix adjust. Identities = 122/362 (33%), Positives = 183/362 (50%), Gaps = 21/362 (5%) Query: 17 IANVIRPVVRGWQSYHVPSAEGMVKLDAMENPYLLPAELREQLAQRLAALELNRYPVPAY 76 I + + P G Q P +VKL+ E+PY P+ L Q R + L YP P Sbjct: 8 IVSRLTPYTPGEQ----PKIARLVKLNTNESPYG-PSPLALQAIAREVSDSLRLYPNPDA 62 Query: 77 TALKAAICKRL---GVPAGYDVLLGNGSDELIALLSVACAQPGRTILAPEPGFVMYGVSA 133 LK AI +R GV +V +GNGSDE++A A Q G+ IL P+ + Y A Sbjct: 63 EPLKQAIARRHAADGVSV-REVFVGNGSDEVLAHAFHALLQHGKPILFPDITYSFYPTYA 121 Query: 134 KMAGLEYVGVPLRADLSLDLPAMLAAMEAHKPVITWLGYPNNPTGTLYEMADVLRIVEAA 193 + +EY VPL D +L L +A +I PN PTG L +ADV RI++ Sbjct: 122 GLYQVEYRAVPLAEDFTLRSEDYLGHGQAIGGII--FPNPNAPTGCLLGLADVERILQ-G 178 Query: 194 APYGLVVVDEAYQPFAASTLMPALPQHNNLVLMRTVSK-LGLAGIRLGYLSAAPALLREL 252 P +VVVDEAY F + +P + ++ NL++++T+SK LAG+R+G+ L+ L Sbjct: 179 NPERVVVVDEAYIDFGGQSAIPLVQRYPNLLVVQTLSKSRALAGMRVGFAVGHADLIDAL 238 Query: 253 DKVRPPYNVNVLTE---AAALFVLEHLDVLQAQAARLRQARSALPAELAALDGVQVFPSA 309 ++V+ +N L A A +E D + + +R+AL A+LA L G QV PSA Sbjct: 239 ERVKNSFNSYPLDRPAIAGATAAIEDEDYFRKTCNMVIASRTALAAQLAGL-GFQVLPSA 297 Query: 310 ANFLLIRVPGADKVCASLLAHR--VLVKNAGRMHVLLQNCLRITVSSDEENAMFLAALKT 367 ANFL +R P D + ++V++ + LRIT+ +DE+ + + AL+ Sbjct: 298 ANFLFVRHPQHDAAQLAAALRADGIIVRHFKSERI--SQFLRITIGTDEDCGVLVEALRR 355 Query: 368 AL 369 L Sbjct: 356 HL 357 >gi|300313928|ref|YP_003778020.1| GntR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 472 Score = 63.9 bits (154), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 61/230 (26%), Positives = 107/230 (46%), Gaps = 23/230 (10%) Query: 95 VLLGNGSDELIALLSVACAQPGRTILAPEPGFVMYGVSAKMAGLEYVGVPLRADLSLDLP 154 VL +G+ + + L+ QPG T+L +PG+ ++AGL +GVP R +D Sbjct: 185 VLTTHGATQALDLVIRTMTQPGDTVLIDDPGYCNLIAMLRLAGLNVIGVP-RTPGGVDTD 243 Query: 155 AMLAAMEAHKPVITW-LGYPNNPTGTLYEMADVLRIVEAAAPYGLVVV-DEAYQPFAAST 212 + H P + + NPTGT Y A +R+++AA +G VV D+ ++ +T Sbjct: 244 VLETLARTHHPKMFFTTSVLQNPTGTSYTPACAMRVLQAAERHGFWVVEDDIFRELGQAT 303 Query: 213 --LMPALPQHNNLVLMRTVSKLGLAGIRLGYLSAAPALLREL-----------DKVRPPY 259 ++ AL ++ + + SK +R+GY++ L R++ ++ Sbjct: 304 DPMLAALDGLQRVIYVGSYSKTIAPSLRVGYIACQRELARQIVHTKMVLTLTNSEINERL 363 Query: 260 NVNVLTEAAALFVLEHLDVLQAQAARLRQARSALPAELAALDGVQVFPSA 309 NVLTE H +Q A L A++ L A+L+ + G+ F +A Sbjct: 364 VHNVLTEG------HHRRHVQTLNATLLSAQATLNAKLSEV-GLTPFTAA 406 >gi|300311875|ref|YP_003775967.1| aspartate/tyrosine/aromatic aminotransferase [Herbaspirillum seropedicae SmR1] Length = 431 Score = 61.6 bits (148), Expect = 9e-11, Method: Compositional matrix adjust. Identities = 62/249 (24%), Positives = 112/249 (44%), Gaps = 11/249 (4%) Query: 100 GSDELIALLSVACAQPGRTILAPEPGFVMYGVSAKMAGLEYVGVPLRADLSLDLPAMLAA 159 GS E +A L +A G T+L P P + ++ A +AG V + + A Sbjct: 137 GSKEGLAHLMLATLDKGDTVLVPNPSYPIHIYGAVIAGANIRSVRMSPGVDFFDELERAV 196 Query: 160 MEAH-KPVITWLGYPNNPTGTLYEMADVLRIVEAAAPYGLVVV------DEAYQPFAAST 212 E++ KP + LG+P+NPT E+ R+V+ A + ++VV D + + A + Sbjct: 197 RESYPKPKMMILGFPSNPTAQCVELEFFERVVKLAREHQILVVHDLAYADITFDGWKAPS 256 Query: 213 LMPALPQHNNLVLMRTVSK-LGLAGIRLGYLSAAPALLRELDKVRPPYNVNVLT--EAAA 269 +M V T+SK +AG R+G++ L+ L +++ ++ T + AA Sbjct: 257 IMQVPGAREVAVEFFTLSKSYNMAGWRIGFMVGNARLVAALARIKSYHDYGSFTPVQVAA 316 Query: 270 LFVLE-HLDVLQAQAARLRQARSALPAELAALDGVQVFPSAANFLLIRVPGADKVCASLL 328 + LE ++ A + R+ L L + P A+ ++ R+P + SL Sbjct: 317 IAALEGDQSCVEEIRANYERRRNVLVKGLHEAGWMVDVPKASMYIWARIPEPYRQFGSLE 376 Query: 329 AHRVLVKNA 337 R+L++ A Sbjct: 377 FARILLEQA 385 >gi|300311954|ref|YP_003776046.1| cobalamin biosynthesis protein [Herbaspirillum seropedicae SmR1] Length = 336 Score = 55.5 bits (132), Expect = 6e-09, Method: Compositional matrix adjust. Identities = 86/281 (30%), Positives = 122/281 (43%), Gaps = 37/281 (13%) Query: 47 NPYLLPAELREQLAQRLAALELNRYPVPAYTALKAAICKRLGVPAGYDVLLGNGSDELI- 105 PY PA L A +R P P+ AL+ A C G P +L G+ I Sbjct: 32 QPYPAPAPL---------ADAWHRLPEPS-AALEQAACDYYGAP---RMLAVAGTQAAIQ 78 Query: 106 ALLSVACAQPGR--TILAPEPGFVMYGVSAKMAGLEYVGVPLRA-DLSLDLPAMLAAMEA 162 AL + + GR ++ P + + + G E VP A D ++ P+ + Sbjct: 79 ALPQLRLRRDGRPAQVVVAAPIYAEHAHCWRRCGHEVSEVPYEALDQAVQSPSCQVLV-- 136 Query: 163 HKPVITWLGYPNNPTGTLYEMADVLRIV-EAAAPYGLVVVDEAYQPFAASTLMPALPQHN 221 L PNNPTG L E +LR + A G +VVDEA+ + A Sbjct: 137 -------LCNPNNPTGALVEPERLLRWAAQLAERGGWLVVDEAFGDTLTHYSVAAHTPQT 189 Query: 222 NLVLMRTVSKL-GLAGIRLGYLSAAPALLRELDKVRPPYNVNVLTEAAALFVLEHLDV-- 278 L+++R+V K GLAG+RLG+++AAPALL EL P+ ++ + L D Sbjct: 190 GLIVLRSVGKFFGLAGLRLGFVAAAPALLAELADWLGPWTISGPAQQIGCAALRDRDWQA 249 Query: 279 -----LQAQAARLRQ--ARSALPAELAALDGVQVFPSAANF 312 LQAQ RL+Q AR + A+ AL P A F Sbjct: 250 QTRQRLQAQGQRLQQLLARHGIAAQGTALFQWWPEPQAQAF 290 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,131,346 Number of extensions: 46570 Number of successful extensions: 206 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 204 Number of HSP's successfully gapped: 5 Length of query: 372 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 285 Effective length of database: 1,207,925 Effective search space: 344258625 Effective search space used: 344258625 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)