BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0081.p (445 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313342|ref|YP_003777434.1| major facilitator superfamily p... 875 0.0 gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 393 e-110 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 375 e-105 gi|300311164|ref|YP_003775256.1| major facilitator superfamily p... 332 5e-92 gi|300313738|ref|YP_003777830.1| major facilitator superfamily p... 313 2e-86 >gi|300313342|ref|YP_003777434.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 875 bits (2262), Expect = 0.0, Method: Compositional matrix adjust. Identities = 445/445 (100%), Positives = 445/445 (100%) Query: 1 MANASQGSAVQFDGLYKKVTWKILPWLLLGYVIAYLDRVNVGFAKLSMLSDLGMSEAAYG 60 MANASQGSAVQFDGLYKKVTWKILPWLLLGYVIAYLDRVNVGFAKLSMLSDLGMSEAAYG Sbjct: 1 MANASQGSAVQFDGLYKKVTWKILPWLLLGYVIAYLDRVNVGFAKLSMLSDLGMSEAAYG 60 Query: 61 LGAGIFFLGYFIFEVPSNLILHRVGAKIWIARIMITWGMISSLMAFTAPIAQWLGVSNET 120 LGAGIFFLGYFIFEVPSNLILHRVGAKIWIARIMITWGMISSLMAFTAPIAQWLGVSNET Sbjct: 61 LGAGIFFLGYFIFEVPSNLILHRVGAKIWIARIMITWGMISSLMAFTAPIAQWLGVSNET 120 Query: 121 VFYTLRFLLGASEAGFFPGVILYMTYWYPAAMHSRVMAVVLLGNPFSYILGGPISGALMQ 180 VFYTLRFLLGASEAGFFPGVILYMTYWYPAAMHSRVMAVVLLGNPFSYILGGPISGALMQ Sbjct: 121 VFYTLRFLLGASEAGFFPGVILYMTYWYPAAMHSRVMAVVLLGNPFSYILGGPISGALMQ 180 Query: 181 FMGSGDSLAGWQWMFVIEALPACLLGIVILFVLSNDIASAKWLAPEEKAELARRLAAENK 240 FMGSGDSLAGWQWMFVIEALPACLLGIVILFVLSNDIASAKWLAPEEKAELARRLAAENK Sbjct: 181 FMGSGDSLAGWQWMFVIEALPACLLGIVILFVLSNDIASAKWLAPEEKAELARRLAAENK 240 Query: 241 EKVNVPLKSLYALPILWFLALVYLLMMIGSYGINFWAPTIIRDAGVKSVLDIGMISAIPY 300 EKVNVPLKSLYALPILWFLALVYLLMMIGSYGINFWAPTIIRDAGVKSVLDIGMISAIPY Sbjct: 241 EKVNVPLKSLYALPILWFLALVYLLMMIGSYGINFWAPTIIRDAGVKSVLDIGMISAIPY 300 Query: 301 ILGATAMVFITRHAEKAGKHRSYSVAVSMVAGLALVLCTLFPHNIYITLAGIAIAVGGSL 360 ILGATAMVFITRHAEKAGKHRSYSVAVSMVAGLALVLCTLFPHNIYITLAGIAIAVGGSL Sbjct: 301 ILGATAMVFITRHAEKAGKHRSYSVAVSMVAGLALVLCTLFPHNIYITLAGIAIAVGGSL 360 Query: 361 TSIALFWGFPGAYFTGATAAAGIAIINSVGNLGGFVGPYLLGALTTAFGSPRYGLMMLGG 420 TSIALFWGFPGAYFTGATAAAGIAIINSVGNLGGFVGPYLLGALTTAFGSPRYGLMMLGG Sbjct: 361 TSIALFWGFPGAYFTGATAAAGIAIINSVGNLGGFVGPYLLGALTTAFGSPRYGLMMLGG 420 Query: 421 ALFLAGVLIALGGRKRGPAAVTASA 445 ALFLAGVLIALGGRKRGPAAVTASA Sbjct: 421 ALFLAGVLIALGGRKRGPAAVTASA 445 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 393 bits (1010), Expect = e-110, Method: Compositional matrix adjust. Identities = 201/417 (48%), Positives = 283/417 (67%), Gaps = 11/417 (2%) Query: 13 DGLYKKVTWKILPWLLLGYVIAYLDRVNVGFAKLSMLSDLGMSEAAYGLGAGIFFLGYFI 72 D Y KVTW++LP L L YV +YLDRVNVGFAKL ML DL S+ YGLGAGIFFLGYF+ Sbjct: 18 DATYSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGYFV 77 Query: 73 FEVPSNLILHRVGAKIWIARIMITWGMISSLMAFTAPIAQWLGVSNETVFYTLRFLLGAS 132 FEVPSN+ILH+VGA++WIARIMITWG+IS MA+ V+ +FY +RFLLG + Sbjct: 78 FEVPSNMILHKVGARLWIARIMITWGIISGAMAY---------VTTPEMFYIMRFLLGVA 128 Query: 133 EAGFFPGVILYMTYWYPAAMHSRVMAVVLLGNPFSYILGGPISGALMQFMGSGDSLAGWQ 192 EAGFFPGVILY+TYWYPAA ++ A+ + G S ++GGP+SG +M M GWQ Sbjct: 129 EAGFFPGVILYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPGVWGHTGWQ 188 Query: 193 WMFVIEALPACLLGIVILFVLSNDIASAKWLAPEEKAELARRLAAENKEKVNVPLKSLYA 252 WMF++EA+P+ +LG++++ + + I A WL+ EEK L ++ E +K ++ L +++A Sbjct: 189 WMFILEAIPSLILGVMVILYMKDRIRDADWLSEEEKRLLEANISHEESQKEHLSLGAMFA 248 Query: 253 LPILWFLALVYLLMMIGSYGINFWAPTIIRDAGVKSVLDIGMISAIPYILGATAMVFITR 312 +W AL+Y ++G YG++FW PTII+ GV L++G+++AIPY A AMV I R Sbjct: 249 NGRVWLGALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGR 308 Query: 313 HAEKAGKHRSYSVAV-SMVAGLALVLCTLFPHNIYITLAGIAIAVGGSLTSIALFWGFPG 371 A+ A + R + VA+ + + + L+L T + HN + +A + +A G LT++ LFW P Sbjct: 309 SAD-ARRERRWHVAIPAFIGCIGLLLSTQYGHNTTLAMASLTLASVGILTTLPLFWSLPT 367 Query: 372 AYFTGATAAAGIAIINSVGNLGGFVGPYLLGALTTAFGSPRYGLMMLGGALFLAGVL 428 A+ +G AAAGIA+INS+GNL GFV P+L+G L A S G+ +L +L + G+L Sbjct: 368 AFLSGTAAAAGIALINSLGNLAGFVSPFLVGWLKDATQSTNAGMYVLAASLVIGGLL 424 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 375 bits (963), Expect = e-105, Method: Compositional matrix adjust. Identities = 198/407 (48%), Positives = 270/407 (66%), Gaps = 10/407 (2%) Query: 13 DGLYKKVTWKILPWLLLGYVIAYLDRVNVGFAKLSMLSDLGMSEAAYGLGAGIFFLGYFI 72 DGLYKKV W+I+P+L+L YVIAYLDRVNVGFAKL M DLG SE +GLGAG+FF+GYF+ Sbjct: 19 DGLYKKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSQDLGFSETVFGLGAGLFFIGYFL 78 Query: 73 FEVPSNLILHRVGAKIWIARIMITWGMISSLMAFTAPIAQWLGVSNETVFYTLRFLLGAS 132 FEVPSN+++H+VGA+IWIARIMITWG++S+ F V N T FY LRFLLG + Sbjct: 79 FEVPSNILMHKVGARIWIARIMITWGILSAAFMF---------VQNATQFYILRFLLGLA 129 Query: 133 EAGFFPGVILYMTYWYPAAMHSRVMAVVLLGNPFSYILGGPISGALMQFMGSGDSLAGWQ 192 EAGF+PG+ILY+TYW+P+ ++V+AV + G P + ILG P+SG +M L GWQ Sbjct: 130 EAGFYPGIILYLTYWFPSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDAFHQNGGLEGWQ 189 Query: 193 WMFVIEALPACLLGIVILFVLSNDIASAKWLAPEEKAELARRLAAENKEKVNVP-LKSLY 251 WMF+IEA+PA L+G+ + L ND+ SAKWL EEKA L + + K K + ++ Sbjct: 190 WMFLIEAIPAVLIGVATVLYLDNDVKSAKWLNDEEKASLQADIDGDAKGKESKHGFGAIV 249 Query: 252 ALPILWFLALVYLLMMIGSYGINFWAPTIIRDAGVKSVLDIGMISAIPYILGATAMVFIT 311 +W + L+Y ++G YG+ W PT+++ GVK L+IG++SAIP+ AM I Sbjct: 250 KDARVWLMCLIYFSFVMGQYGLTLWMPTLVKATGVKGNLEIGLLSAIPFGCAIIAMNLIG 309 Query: 312 RHAEKAGKHRSYSVAVSMVAGLALVLCTLFPHNIYITLAGIAIAVGGSLTSIALFWGFPG 371 R A++ + R + V +++ G+ V LF N +++A +++A G LT LFW P Sbjct: 310 RSADRMRERRWHLVIPALMGGVGFVGAALFADNTAVSIASLSLAAAGVLTCAPLFWSLPT 369 Query: 372 AYFTGATAAAGIAIINSVGNLGGFVGPYLLGALTTAFGSPRYGLMML 418 A+ +GA AA GIA INSVGNL GFV PYL+G L + G+ ML Sbjct: 370 AFLSGAAAAVGIAAINSVGNLAGFVSPYLIGYLKDLTHNNATGMYML 416 >gi|300311164|ref|YP_003775256.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 332 bits (850), Expect = 5e-92, Method: Compositional matrix adjust. Identities = 189/422 (44%), Positives = 265/422 (62%), Gaps = 10/422 (2%) Query: 16 YKKVTWKILPWLLLGYVIAYLDRVNVGFAKLSMLSDLGMSEAAYGLGAGIFFLGYFIFEV 75 Y+K+ W+++P+L+ +V+A++DRVNVGFAKL ML DL SEA YGLGAGIFF+GYFIFEV Sbjct: 25 YRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAVYGLGAGIFFIGYFIFEV 84 Query: 76 PSNLILHRVGAKIWIARIMITWGMISSLMAFTAPIAQWLGVSNETVFYTLRFLLGASEAG 135 PSNL+L ++GA+ +ARI I WG+ S MA+ V+ T FY LRFLLG EAG Sbjct: 85 PSNLLLEKIGARKTLARITILWGLASMAMAY---------VTTPTSFYILRFLLGIFEAG 135 Query: 136 FFPGVILYMTYWYPAAMHSRVMAVVLLGNPFSYILGGPISGALMQFMGSGDSLAGWQWMF 195 FFPGV+LY+TYW+PA +RV + + + +GGPI+G +M M LA WQW+F Sbjct: 136 FFPGVVLYLTYWFPAQRRARVNGLFMTSFAIAGAVGGPIAGLIMSGMEGVGHLANWQWLF 195 Query: 196 VIEALPACLLGIVILFVLSNDIASAKWLAPEEKAELARRLAAENKEK-VNVPLKSLYALP 254 ++E +P+ L G +L L A+AKWL+ EK + + LAAEN + K Sbjct: 196 ILEGIPSILAGFAVLAWLPERPANAKWLSEAEKNAVTQVLAAENSAPGKHFSFKDACTNY 255 Query: 255 ILWFLALVYLLMMIGSYGINFWAPTIIRDAGVKSVLDIGMISAIPYILGATAMVFITRHA 314 +W A VY ++ G+ I FWAP+II++ GVK L IG+ISAIP+I G AM++ H+ Sbjct: 256 RVWICAAVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISAIPFIAGTLAMIWNGFHS 315 Query: 315 EKAGKHRSYSVAVSMVAGLALVLCTLFPHNIYITLAGIAIAVGGSLTSIALFWGFPGAYF 374 +K G+ R +S +++A + LV + N + L + +A G L + +FW PGA+ Sbjct: 316 DKTGERRLHSAIATLIACIGLVATGMTLGNATLALCSLTLAAIGILAAFPVFWSIPGAFL 375 Query: 375 TGATAAAGIAIINSVGNLGGFVGPYLLGALTTAFGSPRYGLMMLGGALFLAGVLIALGGR 434 G AA GIA+INS+GNL GFV PY++GAL T+ GS GL + +A L+ + R Sbjct: 376 AGTAAAGGIALINSIGNLAGFVAPYMIGALKTSTGSLSSGLYFVAALELVASFLVVVFIR 435 Query: 435 KR 436 K Sbjct: 436 KH 437 >gi|300313738|ref|YP_003777830.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 313 bits (803), Expect = 2e-86, Method: Compositional matrix adjust. Identities = 183/437 (41%), Positives = 260/437 (59%), Gaps = 16/437 (3%) Query: 13 DGLYKKVTWKILPWLLLGYVIAYLDRVNVGFAKLSMLSDLGMSEAAYGLGAGIFFLGYFI 72 D KKV +++P++LL YV++YLDR+NVGFA L+M DLG++ + +GLGAG+FF+GYF Sbjct: 21 DATMKKVMRRLIPFILLCYVVSYLDRINVGFAALTMNKDLGLTPSQFGLGAGLFFIGYFF 80 Query: 73 FEVPSNLILHRVGAKIWIARIMITWGMISSLMAFTAPIAQWLGVSNETVFYTLRFLLGAS 132 FE+PSNL LHR GA++WI+RIMI+WG+IS AF V F RFLLG + Sbjct: 81 FEIPSNLALHRFGARMWISRIMISWGLISMATAF---------VVGPKSFALARFLLGMA 131 Query: 133 EAGFFPGVILYMTYWYPAAMHSRVMAVVLLGNPFSYILGGPISGALMQFMGSGDSLAGWQ 192 EAGF PG+ LY T W+P A + A L+G P + I+G P+SGALM+ G GWQ Sbjct: 132 EAGFTPGIYLYFTQWFPGAWRGKATAFFLIGIPVANIIGSPLSGALMELHGMW-GFKGWQ 190 Query: 193 WMFVIEALPACLLGIVILFVLSNDIASAKWLAPEEKAELARRLAAENK---EKVNVPLKS 249 + ++EALPA LLG++ LF+L + A AKWL+ +EK L L+ E + LK Sbjct: 191 VLLLLEALPAVLLGVMCLFLLPDRPAKAKWLSADEKQWLENELSTEQNVLAARHGNKLKD 250 Query: 250 LYALPILWFLALVYLLMMIGSYGINFWAPTIIRDAGVKSVLDIGMISAIPYILGATAMVF 309 + ++ LA +IGS I W P IIR+ G+ S +G+++AIPY++GA AM Sbjct: 251 AFTNWRVFALAGANFCGIIGSLSIGLWLPQIIREFGLPS-HQVGLVAAIPYLVGAVAMTL 309 Query: 310 ITRHAEKAGKHRSYSVAVSMVAGLALVLCTLFPHNIYITLAGIAIAVGGSLTSIALFWGF 369 R A ++ + + ++A L+L + F H + + I +AV L+ A FW Sbjct: 310 WARLANRSERRLFFVAGAIVLAALSLGISA-FLHTPLLKMLAITVAVASILSFQATFWAI 368 Query: 370 PGAYFTGATAAAGIAIINSVGNLGGFVGPYLLGALTTAFGSPRYGLMMLGGALFLAGVL- 428 P + TG AA G+A+I S+GNLGGFVGP ++G + A Y L+ + GAL L V+ Sbjct: 369 PSGFLTGRAAAGGLALIVSIGNLGGFVGPSVIGFIREATQGFTYPLIFVAGALLLGAVIT 428 Query: 429 IALGGRKRGPAAVTASA 445 +ALG R P +A Sbjct: 429 LALGDPSRVPPTSKETA 445 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.141 0.437 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,281,241 Number of extensions: 52402 Number of successful extensions: 340 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 296 Number of HSP's successfully gapped: 19 Length of query: 445 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 357 Effective length of database: 1,203,190 Effective search space: 429538830 Effective search space used: 429538830 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)