BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0077.p (393 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313338|ref|YP_003777430.1| periplasmic trypsin-like serine... 795 0.0 gi|300313678|ref|YP_003777770.1| periplasmic trypsin-like serine... 263 2e-71 gi|300311256|ref|YP_003775348.1| periplasmic trypsin-like serine... 241 1e-64 gi|300310487|ref|YP_003774579.1| serine proteinase [Herbaspirill... 87 3e-18 >gi|300313338|ref|YP_003777430.1| periplasmic trypsin-like serine protease [Herbaspirillum seropedicae SmR1] Length = 393 Score = 795 bits (2053), Expect = 0.0, Method: Compositional matrix adjust. Identities = 393/393 (100%), Positives = 393/393 (100%) Query: 1 MRRLWLLFAQTVTVGLALWFIVATLKPDWLSGSSSSRARPAQSTVQMQEATPNAPALDSY 60 MRRLWLLFAQTVTVGLALWFIVATLKPDWLSGSSSSRARPAQSTVQMQEATPNAPALDSY Sbjct: 1 MRRLWLLFAQTVTVGLALWFIVATLKPDWLSGSSSSRARPAQSTVQMQEATPNAPALDSY 60 Query: 61 RHAARQAMPSVVNIFTTTEARPQKSPFQNDPFFRKFFGDQFDEQQQDDKQSSLGSGVIVS 120 RHAARQAMPSVVNIFTTTEARPQKSPFQNDPFFRKFFGDQFDEQQQDDKQSSLGSGVIVS Sbjct: 61 RHAARQAMPSVVNIFTTTEARPQKSPFQNDPFFRKFFGDQFDEQQQDDKQSSLGSGVIVS 120 Query: 121 PQGYILTNNHVVEAADKIEVALADGRKASAKVVGIDPETDLAVIKIDLPNLPAITLGHPE 180 PQGYILTNNHVVEAADKIEVALADGRKASAKVVGIDPETDLAVIKIDLPNLPAITLGHPE Sbjct: 121 PQGYILTNNHVVEAADKIEVALADGRKASAKVVGIDPETDLAVIKIDLPNLPAITLGHPE 180 Query: 181 NSSVGDVVLAIGNPFGVGQTVTMGIVSALGRNHLGINTFENFIQTDAAINPGNSGGALVD 240 NSSVGDVVLAIGNPFGVGQTVTMGIVSALGRNHLGINTFENFIQTDAAINPGNSGGALVD Sbjct: 181 NSSVGDVVLAIGNPFGVGQTVTMGIVSALGRNHLGINTFENFIQTDAAINPGNSGGALVD 240 Query: 241 TNGNLLGINTAIYSRTGGNLGIGFAIPMSTAKTVMEAIISHGQVVRGWIGVEPQDITPEL 300 TNGNLLGINTAIYSRTGGNLGIGFAIPMSTAKTVMEAIISHGQVVRGWIGVEPQDITPEL Sbjct: 241 TNGNLLGINTAIYSRTGGNLGIGFAIPMSTAKTVMEAIISHGQVVRGWIGVEPQDITPEL 300 Query: 301 AESFGLGKKTGAIIAGVLKGGPADRAGMRPGDILVSIDDKPVADTTEMLNVIAQLTPGQQ 360 AESFGLGKKTGAIIAGVLKGGPADRAGMRPGDILVSIDDKPVADTTEMLNVIAQLTPGQQ Sbjct: 301 AESFGLGKKTGAIIAGVLKGGPADRAGMRPGDILVSIDDKPVADTTEMLNVIAQLTPGQQ 360 Query: 361 IAMTVLRKSQETKLNITVGKRPPPPKNEDADEE 393 IAMTVLRKSQETKLNITVGKRPPPPKNEDADEE Sbjct: 361 IAMTVLRKSQETKLNITVGKRPPPPKNEDADEE 393 >gi|300313678|ref|YP_003777770.1| periplasmic trypsin-like serine endoprotease [Herbaspirillum seropedicae SmR1] Length = 494 Score = 263 bits (673), Expect = 2e-71, Method: Compositional matrix adjust. Identities = 143/319 (44%), Positives = 201/319 (63%), Gaps = 8/319 (2%) Query: 69 PSVVNIFTTTEARPQKSPFQN-----DPF--FRKFFGDQFDEQQQDDKQSSLGSGVIVSP 121 P+VVNI T +AR N DPF F K FG Q QQ LGSG I+SP Sbjct: 74 PAVVNISVTGKARKVADDDDNGLDPNDPFSEFFKRFGPQLQMPQQPRIMHGLGSGFIISP 133 Query: 122 QGYILTNNHVVEAADKIEVALADGRKASAKVVGIDPETDLAVIKIDLPNLPAITLGHPEN 181 G ILTN HVV+ A ++ V L D R+ AKV+GID ++D+AVI+ID NLP + +G P Sbjct: 134 DGLILTNAHVVDGAQEVVVKLTDRREFKAKVLGIDKQSDIAVIRIDAKNLPTVQIGDPSR 193 Query: 182 SSVGDVVLAIGNPFGVGQTVTMGIVSALGRNHLGINTFENFIQTDAAINPGNSGGALVDT 241 VG VLAIG+P+G T T GI+SA R+ L + + FIQTD A+NPGNSGG L D Sbjct: 194 VKVGQPVLAIGSPYGFDNTATAGIISAKSRS-LPDDNYVPFIQTDVAVNPGNSGGPLFDL 252 Query: 242 NGNLLGINTAIYSRTGGNLGIGFAIPMSTAKTVMEAIISHGQVVRGWIGVEPQDITPELA 301 NG ++GIN+ IYS+TGG G+ F+IP+ A V + +++HG+V RG +GV QD+ L+ Sbjct: 253 NGQVIGINSQIYSQTGGFQGLSFSIPIDVAMKVEQQLVTHGKVTRGRLGVSVQDLNQALS 312 Query: 302 ESFGLGKKTGAIIAGVLKGGPADRAGMRPGDILVSIDDKPVADTTEMLNVIAQLTPGQQI 361 +SFGL K GA+++ V KG PAD+AG++ GD+++S + + + ++ ++A PG Sbjct: 313 DSFGLKKAEGALVSSVEKGSPADKAGLQAGDVIISFNGHAIDHSVDLPTLVADTPPGSTK 372 Query: 362 AMTVLRKSQETKLNITVGK 380 M V+R + +N+TVG+ Sbjct: 373 PMEVMRAGKVRTINVTVGE 391 >gi|300311256|ref|YP_003775348.1| periplasmic trypsin-like serine protease [Herbaspirillum seropedicae SmR1] Length = 492 Score = 241 bits (614), Expect = 1e-64, Method: Compositional matrix adjust. Identities = 138/351 (39%), Positives = 205/351 (58%), Gaps = 27/351 (7%) Query: 51 TPNAPALDSYRHAARQAMPSVVNIFTTTEARPQKSPFQ---NDP----FFRKFFGDQFDE 103 +P AL + + P VVNI TT R Q + Q +DP FFR+FFG Sbjct: 32 SPAVAALPDFADIVERTGPGVVNIRTTERVR-QNAQGQGGVDDPEMQEFFRRFFGIPIPR 90 Query: 104 QQQ----------------DDKQSSLGSGVIVSPQGYILTNNHVVEAADKIEVALADGRK 147 QQQ ++ +GSG I+S G+I+TN HVVE A ++ V L D R+ Sbjct: 91 QQQPGTPRGNGKQGGQGQQEEVPRGVGSGFIISADGFIMTNAHVVEGASEVYVTLTDKRE 150 Query: 148 ASAKVVGIDPETDLAVIKIDLPNLPAITLGHPENSSVGDVVLAIGNPFGVGQTVTMGIVS 207 AK+VG D TD+AV+KID NLP + +G + VG+ VLAIG+PFG+ TVT GIVS Sbjct: 151 FKAKIVGSDTRTDVAVLKIDGSNLPRLNMGDSDKIRVGEWVLAIGSPFGLENTVTAGIVS 210 Query: 208 ALGRNHLGINTFENFIQTDAAINPGNSGGALVDTNGNLLGINTAIYSRTGGNLGIGFAIP 267 A R+ + IQTD A+NPGNSGG L++ G ++GIN+ IYSR+GG +GI FA+P Sbjct: 211 AKARDT---GDYLPLIQTDVAVNPGNSGGPLINLKGEVIGINSQIYSRSGGFMGISFAVP 267 Query: 268 MSTAKTVMEAIISHGQVVRGWIGVEPQDITPELAESFGLGKKTGAIIAGVLKGGPADRAG 327 + A V + + + G+V RG IGV+ ++T ++AES GL + GA++ V GGPA++AG Sbjct: 268 IDEALRVADQLKASGRVTRGRIGVQIGEVTKDVAESLGLARAQGALVQRVEAGGPAEKAG 327 Query: 328 MRPGDILVSIDDKPVADTTEMLNVIAQLTPGQQIAMTVLRKSQETKLNITV 378 + GDI++ + + +++ ++ PG + +++ RK +++TV Sbjct: 328 LEAGDIILKYNGAAIERPSDLPRMVGSTKPGAKATVSIWRKGSARDVSVTV 378 >gi|300310487|ref|YP_003774579.1| serine proteinase [Herbaspirillum seropedicae SmR1] Length = 438 Score = 87.0 bits (214), Expect = 3e-18, Method: Compositional matrix adjust. Identities = 56/163 (34%), Positives = 94/163 (57%), Gaps = 15/163 (9%) Query: 114 GSGVIVSPQGYILTNNHVVEAADKIEVALADGRKASAKVVGIDPETDLAVIKIDLPNLPA 173 G+G + G++L+N HVV A + V L GR+ A+V+ +D D+A++K + P + Sbjct: 271 GTGFYIGQSGWLLSNQHVVGTARSVRVRLPGGRELPAEVLRVDAARDVALLKTEPPGVRP 330 Query: 174 ITLGHPENSSVGDVVLAIGNPFG--VGQTVTMGIVSALGRNHLGINTF--ENFIQTDAAI 229 + L + +G+ V A+G+P G T+T GI+S G+ + F+Q+D AI Sbjct: 331 LPL-RLDEPGLGEDVYALGSPLGDTFNTTLTRGILS-------GVRLLNGQEFLQSDVAI 382 Query: 230 NPGNSGGALVDTNGNLLGINTA-IYSRTGGNLGIGFAIPMSTA 271 PG+SGG L+D +G ++GI A + +R G G+ F IP+++A Sbjct: 383 LPGSSGGPLLDRSGQVVGITVAGLGAR--GMAGMNFFIPLASA 423 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.133 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,292,187 Number of extensions: 56124 Number of successful extensions: 280 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 275 Number of HSP's successfully gapped: 4 Length of query: 393 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 306 Effective length of database: 1,207,925 Effective search space: 369625050 Effective search space used: 369625050 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 131 (55.1 bits)