BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0069.p (501 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313329|ref|YP_003777421.1| transmembrane protein [Herbaspi... 995 0.0 gi|300313918|ref|YP_003778010.1| transmembrane protein [Herbaspi... 608 e-175 gi|300313782|ref|YP_003777874.1| transmembrane protein [Herbaspi... 409 e-115 gi|300312843|ref|YP_003776935.1| response regulator containing a... 194 9e-51 gi|300310865|ref|YP_003774957.1| response regulator containing a... 193 3e-50 >gi|300313329|ref|YP_003777421.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 501 Score = 995 bits (2573), Expect = 0.0, Method: Compositional matrix adjust. Identities = 489/501 (97%), Positives = 494/501 (98%) Query: 1 MGRLQKYLALILGVIIMMVLTICGASLYEGYRQTRISAITEANNVTLIVQRAISRNMEIL 60 MGRLQKYLALILG+IIMMVL+ICGASLYEGYRQTRISAITEANNVTLIVQRAISRNMEIL Sbjct: 1 MGRLQKYLALILGLIIMMVLSICGASLYEGYRQTRISAITEANNVTLIVQRAISRNMEIL 60 Query: 61 SLAMDTLAYRYQHPLDLTNASDEERYNYLFGSTAAAHHITALAILDADGYVRVTSRRSAS 120 SLAMDTLAYRYQHPLDLT+ASDEERYNYLFGSTAAAHHITALAILDADGYVRVTSRRSAS Sbjct: 61 SLAMDTLAYRYQHPLDLTHASDEERYNYLFGSTAAAHHITALAILDADGYVRVTSRRSAS 120 Query: 121 PSVVVNYSERTYFTVHRDSADAGLFISRPIKANLGNELPVVVLSKRLSRKDGSFDGVVLM 180 PSVVVNYSERTYFTVHRDSADAGLFISRPIKANLGNELPVVVLSKRLSRKDGSFDGVVLM Sbjct: 121 PSVVVNYSERTYFTVHRDSADAGLFISRPIKANLGNELPVVVLSKRLSRKDGSFDGVVLM 180 Query: 181 ALDMSYFRDLFTGLTLGEDGIMSLYSDDGVTYMRVPYHPSSIGREITNNPNFQRLRAMLV 240 ALDMSYFRDLFTGLTLGEDGIMSLYSDDGVTYMRVPYHPSSIGREITNNPNFQRLRAMLV Sbjct: 181 ALDMSYFRDLFTGLTLGEDGIMSLYSDDGVTYMRVPYHPSSIGREITNNPNFQRLRAMLV 240 Query: 241 DNDGNFFARAHTDGVERLYTFRRIPDAPLIVLLGRSERFIFKTWYDTLYSVLLLLIIFTA 300 DNDGNFFARAHTDGVERLYTFRRIPDAPLIVLLGRSERFIFKTWYDTLYSVLLLLIIFTA Sbjct: 241 DNDGNFFARAHTDGVERLYTFRRIPDAPLIVLLGRSERFIFKTWYDTLYSVLLLLIIFTA 300 Query: 301 LAGVLLMLVRRELLKRVAAEKQLEDLARTDGLTGLLNRRSLDEALLAVWQRSQRSPTVGF 360 LAGVLLMLVRRELLKRVAAEKQLEDLARTDGLTGLLNRRSLDEALLAVWQRSQRSPT GF Sbjct: 301 LAGVLLMLVRRELLKRVAAEKQLEDLARTDGLTGLLNRRSLDEALLAVWQRSQRSPTAGF 360 Query: 361 STLFIDVDHFKRYNDTYGHEMGDTVLQAVAAVLRAHLQRQNDLAGRYGGEEFVVLLEQTD 420 STLFIDVD+FKRYNDTYGHEMGDTVLQAVAAVLR HLQRQNDLAGRYGGEEFVVLLEQTD Sbjct: 361 STLFIDVDYFKRYNDTYGHEMGDTVLQAVAAVLREHLQRQNDLAGRYGGEEFVVLLEQTD 420 Query: 421 EQGALRMAQQLCEAIYSRGIPHSGSPLGVISVSIGVATMNRARHQRMEDVLNAADHAPYI 480 E GALRMAQQLCEAIY+RGIPHSGSPLGVISVSIGVATMNRA HQRMEDVLNAADHA YI Sbjct: 421 EHGALRMAQQLCEAIYNRGIPHSGSPLGVISVSIGVATMNRAPHQRMEDVLNAADHALYI 480 Query: 481 AKREGRNRVCVAHPCQNDPPA 501 AKREGRNRVCVAHP QNDP A Sbjct: 481 AKREGRNRVCVAHPGQNDPLA 501 >gi|300313918|ref|YP_003778010.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 515 Score = 608 bits (1569), Expect = e-175, Method: Compositional matrix adjust. Identities = 298/492 (60%), Positives = 380/492 (77%), Gaps = 7/492 (1%) Query: 1 MGRLQKYLALILGVIIMMVLTICGASLYEGYRQTRISAITEANNVTLIVQRAISRNMEIL 60 + +L+ YL +IL + ++MV+ I ++LY+ Y+QTR SA++ A+NVTL+ QRAISRN+E+L Sbjct: 22 IKQLKNYLVVILAIAVLMVMAISASTLYQSYQQTRQSAVSNASNVTLVSQRAISRNLEML 81 Query: 61 SLAMDTLAYRYQHPLDLTNASDEERYNYLFGSTAAAHHITALAILDADGYVRVTSR---R 117 SL++DTLAYR+QHPL +D +RY YLFGST HI +A+LDADG V+V+SR Sbjct: 82 SLSLDTLAYRFQHPLSARRLNDAQRYAYLFGSTTNVSHIAVMAVLDADGRVQVSSRLGRG 141 Query: 118 SASPSVVVNYSERTYFTVHRDSADAGLFISRPIKANLGNELPVVVLSKRLSRKDGSFDGV 177 + +P +Y++R YF HRDS GL++SRP++A+LGNEL VVVLSKRLS +DGSF GV Sbjct: 142 NGAP----HYADRAYFIAHRDSPTVGLYVSRPMQASLGNELQVVVLSKRLSNEDGSFAGV 197 Query: 178 VLMALDMSYFRDLFTGLTLGEDGIMSLYSDDGVTYMRVPYHPSSIGREITNNPNFQRLRA 237 V+MALD++YFR+LF GL LG++GI+SLYSD+G+ YMRVPY + IGR+I+ NFQRLRA Sbjct: 198 VVMALDLAYFRELFEGLALGDEGIISLYSDEGIAYMRVPYRETIIGRDISQTGNFQRLRA 257 Query: 238 MLVDNDGNFFARAHTDGVERLYTFRRIPDAPLIVLLGRSERFIFKTWYDTLYSVLLLLII 297 ML + +G+FFA A DGV RLYTFRRIP +PLIV +G +E IFKTW T Y+VL+ L I Sbjct: 258 MLSEEEGSFFAHATNDGVRRLYTFRRIPGSPLIVFVGYAEEAIFKTWRKTFYAVLVSLAI 317 Query: 298 FTALAGVLLMLVRRELLKRVAAEKQLEDLARTDGLTGLLNRRSLDEALLAVWQRSQRSPT 357 F L LL+ VR+EL +RV AE+QLE LARTDGLT LLNRR+LDEAL A W+R QR+ Sbjct: 318 FGLLTAWLLLHVRQELRRRVTAERQLEALARTDGLTDLLNRRALDEALQATWERCQRNLG 377 Query: 358 VGFSTLFIDVDHFKRYNDTYGHEMGDTVLQAVAAVLRAHLQRQNDLAGRYGGEEFVVLLE 417 FS LFIDVD+FK YNDTYGH++GDTVL+ VAA L A L R D AGRYGGEEFVVLLE Sbjct: 378 ASFSVLFIDVDYFKAYNDTYGHKLGDTVLKEVAATLTARLPRHTDCAGRYGGEEFVVLLE 437 Query: 418 QTDEQGALRMAQQLCEAIYSRGIPHSGSPLGVISVSIGVATMNRARHQRMEDVLNAADHA 477 QTD QGA MAQ+LC+A+ R I H+ SPL V+++S+GVAT+ R H+R+EDVLNAAD A Sbjct: 438 QTDLQGAQMMAQRLCDAVRERHIAHTSSPLAVVTISVGVATLERDHHRRVEDVLNAADAA 497 Query: 478 PYIAKREGRNRV 489 Y AK+ GR+RV Sbjct: 498 LYRAKQAGRDRV 509 >gi|300313782|ref|YP_003777874.1| transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 475 Score = 409 bits (1050), Expect = e-115, Method: Compositional matrix adjust. Identities = 216/474 (45%), Positives = 303/474 (63%), Gaps = 5/474 (1%) Query: 18 MVLTICGASLYEGYRQTRISAITEANNVTLIVQRAISRNMEILSLAMDTLAYRYQHPLDL 77 M T+ A LY+ +RQ +AI N+ I QR+ISRN+E+L+LA+D+L +RY+H L Sbjct: 1 MTATVSCAVLYQSHRQVERAAIEHGANIARIAQRSISRNVELLTLALDSLTWRYRHT-QL 59 Query: 78 TNASDEERYNYLFGSTAAAHHITALAILDADGYVRVTSRRSASPSVVVNYSERTYFTVHR 137 +++ +L G A ++ AL I+D DG + V S + S + Y++ +FT H+ Sbjct: 60 HEMPLQQKMEFLLGENIEARYVAALGIVDTDGELVVGSA-ALSQILPGPYAQSDFFTAHQ 118 Query: 138 D---SADAGLFISRPIKANLGNELPVVVLSKRLSRKDGSFDGVVLMALDMSYFRDLFTGL 194 D GL+IS P+ L + VVVLSKRL++ DGSF G+ LM L + YFRDLF GL Sbjct: 119 THHAQGDTGLYISAPLTVRLERRMQVVVLSKRLAQPDGSFAGISLMVLYLDYFRDLFQGL 178 Query: 195 TLGEDGIMSLYSDDGVTYMRVPYHPSSIGREITNNPNFQRLRAMLVDNDGNFFARAHTDG 254 TL DG++SLYS DG+ YMR+PY+ + IG + + + +R+ + G++FARA DG Sbjct: 179 TLENDGVISLYSADGLAYMRIPYNEAFIGSRLNISEDLERIFSPSGPAQGSYFARAQQDG 238 Query: 255 VERLYTFRRIPDAPLIVLLGRSERFIFKTWYDTLYSVLLLLIIFTALAGVLLMLVRRELL 314 LYTF R+P V +GR+ IF+ WY+ LY+VL L+ F + L+ VR EL Sbjct: 239 PRWLYTFARVPGTGWTVFIGRTGARIFREWYNMLYAVLFLMASFCIASIYLIRRVRVELQ 298 Query: 315 KRVAAEKQLEDLARTDGLTGLLNRRSLDEALLAVWQRSQRSPTVGFSTLFIDVDHFKRYN 374 +R A ++QLE+LARTD LT LLNRR+LDEAL W+R++ +P FS LF+DVD FK YN Sbjct: 299 RRSALDQQLEELARTDKLTSLLNRRALDEALARAWKRAEENPDAIFSLLFVDVDFFKLYN 358 Query: 375 DTYGHEMGDTVLQAVAAVLRAHLQRQNDLAGRYGGEEFVVLLEQTDEQGALRMAQQLCEA 434 DTYGH+ GD L AV+ + A + R +D +GRYGGEEFVVLL TD GA +A+++ A Sbjct: 359 DTYGHKAGDYALMAVSRCIVAAVSRLSDQSGRYGGEEFVVLLNGTDTVGATHVAKRIISA 418 Query: 435 IYSRGIPHSGSPLGVISVSIGVATMNRARHQRMEDVLNAADHAPYIAKREGRNR 488 I S IPH SP +++VSIGV ++ R H +EDV+ AD A Y AK++GRN+ Sbjct: 419 IQSLKIPHERSPFDILTVSIGVTSLLREHHSSIEDVVKDADAALYRAKQDGRNK 472 >gi|300312843|ref|YP_003776935.1| response regulator containing a CheY-like receiver and a GGDEF domains protein [Herbaspirillum seropedicae SmR1] Length = 525 Score = 194 bits (494), Expect = 9e-51, Method: Compositional matrix adjust. Identities = 144/418 (34%), Positives = 223/418 (53%), Gaps = 22/418 (5%) Query: 98 HITALAILDADGYVRVTSRRSASPSVVVNYSERTYFTVHRDSADAGLFISRPIKANLGNE 157 + L + D G +TS SA P N S+R YF HRD D G + + + + Sbjct: 109 QLDGLYVFDEHGKRLLTS--SAPPLAPANNSDRDYFIWHRDHDDVGPHVGKALLSRSTGR 166 Query: 158 LPVVVLSKRLSRKDGSFDGVVLMALDMSYFRDLFTGLTLGEDGIMSLYSDDGVTYMRVPY 217 ++ +S+RL+ DGSF GV+L LD+++F L+ + +G+ G ++L DG R+P+ Sbjct: 167 W-IIPMSRRLNHPDGSFAGVLLATLDINHFNQLYRTVEIGKGGSIALLLRDGTLLTRMPF 225 Query: 218 HPSSIGREITNNPNFQRLRAMLVDNDGNFFARAHTDGVERLYTFRRIPDAPLIVLLGRSE 277 P I R+ + F L + G + D +R +++ + PL++++ + Sbjct: 226 DPGYINRDFSQGRLF--LEGLPHSPFGYLEVVSPLDARQRFLSYQAVDKYPLVLVVTLAR 283 Query: 278 RFIFKTWYDTLY----SVLLLLIIFTA----LAGVLLMLVRRE------LLKRVAAEKQL 323 W + +VLLLL I L G + + +R E + + A +L Sbjct: 284 EEALADWKQQTFVYGAAVLLLLAIIGVFGWRLVGQIELRLRAEDRALQAMGELQMANHRL 343 Query: 324 EDLARTDGLTGLLNRRSLDEALLAVWQRSQRSPTVGFSTLFIDVDHFKRYNDTYGHEMGD 383 E LA DGLTGL NRR LD L ++R++RS + + IDVD FK+YND YGH+ GD Sbjct: 344 EMLAHQDGLTGLANRRHLDTMLETEFKRAERS-GAALALIMIDVDFFKQYNDLYGHQAGD 402 Query: 384 TVLQAVAAVLRAHLQRQNDLAGRYGGEEFVVLLEQTDEQGALRMAQQLCEAIYSRGIPHS 443 L+ VA VL+ +R DLA RYGGEE +LL TD +GA +A++L I + +PH Sbjct: 403 ECLRRVAGVLKERQRRPGDLAARYGGEEMAMLLPGTDAEGACAVAEKLRAGIQALELPHR 462 Query: 444 GSPLGVISVSIGVATMN--RARHQRMEDVLNAADHAPYIAKREGRNRVCVAHPCQNDP 499 G+P+GV++VS+GV + + + ++ +L AAD A Y AK EGRN+V +A P + P Sbjct: 463 GNPVGVVTVSVGVCALELLPSAARSVQALLGAADAALYEAKHEGRNQVRLAAPMKTAP 520 >gi|300310865|ref|YP_003774957.1| response regulator containing a CheY-like receiver domain and a GGDEF domain transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 554 Score = 193 bits (490), Expect = 3e-50, Method: Compositional matrix adjust. Identities = 137/471 (29%), Positives = 243/471 (51%), Gaps = 31/471 (6%) Query: 38 AITEANNVTLIVQRAISRNMEILSLAMDTLAYRYQHPLDLTNASDEERYNYLFGSTAAAH 97 + T+A+NV L + + ++++ + ++T+ H D + ++ ++++ S A+ Sbjct: 56 STTDASNVALALIQHAEQSIKEADIVLETVQEHLDHE-DRSASARIRTHDFMIRSVASLP 114 Query: 98 HITALAILDADGYVRVTSRR----SASPSVVVNYSERTYFTVHRDSADAGLFISRPIKAN 153 + L ++D G +S++ + SP + YF H+ FI +P++++ Sbjct: 115 QLAQLTVVDEQGRTVASSQQRMVQTGSPV------QDDYFQYHQHRRSDRPFIGQPMRSH 168 Query: 154 LGNELPVVVLSKRLSRKDGSFDGVVLMALDMSYFRDLFTGLTLGEDGIMSLYSDDGVTYM 213 + + + V S+R++ DGSF GV++ +D++YF+ + +G G++ G + Sbjct: 169 VTGQRTLTV-SRRINHVDGSFAGVIVAEIDLAYFQRYYEQFNIGPHGVILFGLTGGSLIL 227 Query: 214 RVPYHPSSIGREITNNPNFQRLRAMLVDNDGNFFARAHTDGVERLYTFRRIPDAPLIVLL 273 R P R + ++ M G+F R+ DGVER+ F++ P+ ++ Sbjct: 228 RRPLLADMTDRSPAMSEALRK--HMEQARRGSFAMRSERDGVERISCFQQGEAYPIFTVV 285 Query: 274 GRSERFIFKTWYDTLY----SVLLLLIIFTALAGVLLMLV---RRELLKRVAAEKQLEDL 326 ++ I + W ++ V+LLL + L++LV R + +AA ++LE L Sbjct: 286 ALAKDDILEDWSRHVWVRGIGVVLLLAVICFGGKRLVVLVAGRERAEAQAIAARQELESL 345 Query: 327 ART-------DGLTGLLNRRSLDEALLAVWQRSQRSPTVGFSTLFIDVDHFKRYNDTYGH 379 RT DGLT + NRR D AL A R R+ S + IDVDHFK++NDTYGH Sbjct: 346 YRTLEAQSQKDGLTDVFNRRYFDAALAAELARLNRTGE-SLSLMMIDVDHFKQFNDTYGH 404 Query: 380 EMGDTVLQAVAAVLRAHLQRQNDLAGRYGGEEFVVLLEQTDEQGALRMAQQLCEAIYSRG 439 GD L+ VAA + +RQ D+ RYGGEEF V+L A +A ++ +A+ Sbjct: 405 AAGDVCLRQVAAAISTVARRQGDIVARYGGEEFAVILPNCHPGSAQGLAHRVVQAVRGLE 464 Query: 440 IPHSGSPLGVISVSIGVATMNRARHQRME--DVLNAADHAPYIAKREGRNR 488 I H SP+GV+++S+GVA++ + +E +++++AD A Y AK EGR+R Sbjct: 465 IAHERSPMGVVTISVGVASLASGKSLLIEPRELIDSADGALYRAKEEGRDR 515 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.137 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,444,954 Number of extensions: 58430 Number of successful extensions: 281 Number of sequences better than 1.0e-08: 42 Number of HSP's gapped: 199 Number of HSP's successfully gapped: 42 Length of query: 501 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 412 Effective length of database: 1,198,455 Effective search space: 493763460 Effective search space used: 493763460 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 133 (55.8 bits)