BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0068.p (564 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313312|ref|YP_003777404.1| methyl-accepting chemotaxis pro... 1029 0.0 gi|300312220|ref|YP_003776312.1| methyl-accepting chemotaxis tra... 452 e-128 gi|300312219|ref|YP_003776311.1| methyl-accepting chemotaxis tra... 428 e-121 gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis pro... 393 e-110 gi|300311614|ref|YP_003775706.1| methyl-accepting chemotaxis tra... 377 e-105 >gi|300313312|ref|YP_003777404.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 544 Score = 1029 bits (2660), Expect = 0.0, Method: Compositional matrix adjust. Identities = 525/540 (97%), Positives = 525/540 (97%) Query: 24 MKLSIVNRLYILIGAVIIGCVVLAGANMVKMNRTYADASFASENVVPSLLSLSTITQGIN 83 MKLSIVNRLYILIGAVIIGCVVLAGANMVKMNRTYADASFASENVVPSLLSLSTITQGIN Sbjct: 1 MKLSIVNRLYILIGAVIIGCVVLAGANMVKMNRTYADASFASENVVPSLLSLSTITQGIN 60 Query: 84 GMRIATWRYLALTESVDRGAIESSFNSAKKRVEDALQKYEQEQLADATDKELLRAVRVSY 143 GMRIATWRYLALTESVDRGAIESSFNSAKKRVEDALQKYEQEQLADATDKELLRAVRVSY Sbjct: 61 GMRIATWRYLALTESVDRGAIESSFNSAKKRVEDALQKYEQEQLADATDKELLRAVRVSY 120 Query: 144 QEFDKSLAEALKLANAGQWNEARALLANNQPVIDRLNKAMTELRAYNEKISQAGAHRAQE 203 QEFDKSLAEALKLANAGQWNEARALLANNQPVIDRLNKAMTELRAYNEKISQAGAHRAQE Sbjct: 121 QEFDKSLAEALKLANAGQWNEARALLANNQPVIDRLNKAMTELRAYNEKISQAGAHRAQE 180 Query: 204 TMAAARLHAILISAAVIAVVTLMGLLIARKIARSLAEAVELAETIAEGDLTREIDISSTD 263 TMAAARLHAILISAAVIAVVTLMGLLIARKIARSLAEAVELAETIAEGDLTREID SSTD Sbjct: 181 TMAAARLHAILISAAVIAVVTLMGLLIARKIARSLAEAVELAETIAEGDLTREIDTSSTD 240 Query: 264 EIGHLMAAMAKMNRNLVTIVATVRTGVNTVAAASSQIATGNMDLSARTEQQASSLEQTAA 323 EIGHLMAAMAKMNRNLVTIVATVRTGVNTVAAASSQIATGNMDLSARTEQQASSLEQTAA Sbjct: 241 EIGHLMAAMAKMNRNLVTIVATVRTGVNTVAAASSQIATGNMDLSARTEQQASSLEQTAA 300 Query: 324 ATEELTSTVKQNAENALQADLLASAASGIAAEGGEVVKQVVTTMEEIRSSSLNIADIISV 383 ATEELTSTVKQNAENALQAD LASAASGIAAEGGEVVKQVVTTMEEIRSSSLNIADIISV Sbjct: 301 ATEELTSTVKQNAENALQADQLASAASGIAAEGGEVVKQVVTTMEEIRSSSLNIADIISV 360 Query: 384 IDGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSASAAKEIKQLIDDSVSK 443 IDGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSASAAKEIKQLIDDSVSK Sbjct: 361 IDGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSASAAKEIKQLIDDSVSK 420 Query: 444 VENGSQLVMSAGNTMQQVVESVRRVTDVVTEISAASREQSLGIEQVNQAISHMDQSTQEN 503 VENGSQLVMSAGNTMQQVVESVRRVTDVVTEISAASREQSLGIEQVNQAISHMDQSTQEN Sbjct: 421 VENGSQLVMSAGNTMQQVVESVRRVTDVVTEISAASREQSLGIEQVNQAISHMDQSTQEN 480 Query: 504 AALVEEATAAAQSLQEQARQLERAVNVFKLRQEAGAILEMEDRHTTHALSSPALPKLPAP 563 AALVEEATAAAQSLQEQARQLERAVNVFKLRQEA ILEMED T L L LPAP Sbjct: 481 AALVEEATAAAQSLQEQARQLERAVNVFKLRQEAMDILEMEDHRVTQTLPGRELSLLPAP 540 >gi|300312220|ref|YP_003776312.1| methyl-accepting chemotaxis transducer transmembrane I protein [Herbaspirillum seropedicae SmR1] Length = 535 Score = 452 bits (1162), Expect = e-128, Method: Compositional matrix adjust. Identities = 269/509 (52%), Positives = 366/509 (71%), Gaps = 1/509 (0%) Query: 26 LSIVNRLYILIGAVIIGCVVLAGANMVKMNRTYADASFASENVVPSLLSLSTITQGINGM 85 ++I +L++L AVI+G L+ + + AS++S N VPSLL+L + G+ Sbjct: 1 MTIAKKLHLLTLAVILGLASLSLLGIYQSREVAKAASYSSVNTVPSLLALGDAGEAAFGI 60 Query: 86 RIATWRYLALTESVDRGAIESSFNSAKKRVEDALQKYEQEQLADATDKELLRAVRVSYQE 145 R+ W+Y A T+ V R +E + RV A +Y +E ++D TD++LL A R ++ + Sbjct: 61 RVGIWKYAAATDPVSRTRLEKAMAEQHDRVLAAFDRYAREDISDDTDRKLLEADRQAFAD 120 Query: 146 FDKSLAEALKLANAGQWNEA-RALLANNQPVIDRLNKAMTELRAYNEKISQAGAHRAQET 204 ++K A L++ + EA RA+ P D+L A+ E R Y E++ + G A+ Sbjct: 121 YEKQRAVVLRIGAESRNEEALRAIEQLVVPPGDKLIAALAEHRKYKEQLGRQGVETAEGI 180 Query: 205 MAAARLHAILISAAVIAVVTLMGLLIARKIARSLAEAVELAETIAEGDLTREIDISSTDE 264 + ++ AIL+S V A+V LLI+R I+ SL +A+ +A IA GDL+ ++ ++ DE Sbjct: 181 LQSSTYQAILVSLLVAAIVGATSLLISRGISVSLDDAIRVANAIAHGDLSSKVKMTGKDE 240 Query: 265 IGHLMAAMAKMNRNLVTIVATVRTGVNTVAAASSQIATGNMDLSARTEQQASSLEQTAAA 324 +G L+ A+ +M +LV IV VR V + +ASS+IA+GN+DLSARTE QA S+E+TA+A Sbjct: 241 VGQLLHAIGEMTASLVNIVGNVRQSVAAIESASSEIASGNLDLSARTEAQAGSIEETASA 300 Query: 325 TEELTSTVKQNAENALQADLLASAASGIAAEGGEVVKQVVTTMEEIRSSSLNIADIISVI 384 E+LT+TVKQNA+NA QAD LAS+AS +A+EGG+VV++VV TM+ I SSSL I DIISVI Sbjct: 301 MEQLTATVKQNADNAHQADTLASSASSVASEGGKVVQEVVHTMDAINSSSLKIVDIISVI 360 Query: 385 DGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSASAAKEIKQLIDDSVSKV 444 DGIAFQTNILALNAAVEAARAGEQGRGFAVVA+EVRSLAQRSASAA+EIK+LIDDSV KV Sbjct: 361 DGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAREIKELIDDSVEKV 420 Query: 445 ENGSQLVMSAGNTMQQVVESVRRVTDVVTEISAASREQSLGIEQVNQAISHMDQSTQENA 504 ++GS+LVM AGNTM+QVV SV+RV+D+V +IS AS EQS GI QVNQAI MDQ+TQ+NA Sbjct: 421 QSGSRLVMQAGNTMEQVVTSVQRVSDIVRDISVASGEQSDGIAQVNQAIGMMDQTTQQNA 480 Query: 505 ALVEEATAAAQSLQEQARQLERAVNVFKL 533 ALVEEA AA+QSL+EQA+ L +AV+VF+L Sbjct: 481 ALVEEAAAASQSLREQAKGLAQAVSVFRL 509 >gi|300312219|ref|YP_003776311.1| methyl-accepting chemotaxis transducer transmembrane I protein [Herbaspirillum seropedicae SmR1] Length = 563 Score = 428 bits (1101), Expect = e-121, Method: Compositional matrix adjust. Identities = 249/515 (48%), Positives = 356/515 (69%), Gaps = 3/515 (0%) Query: 26 LSIVNRLYILIGAVIIGCVVLAGANMVKMNRTYADASFASENVVPSLLSLSTITQGINGM 85 ++I +LY+LI AV++G ++ A + + +R + A +++ + +PS+L+L+ T I G+ Sbjct: 31 MTIARKLYLLIVAVVLGVIMQAVVGLYQSHRIASSAGYSATDTIPSVLALNGATDAIYGI 90 Query: 86 RIATWRYLALTESVDRGAIESSFNSAKKRVEDALQKYEQEQLADATDKELLRAVRVSYQE 145 R+ RY+ E R ++ + ++ +D ++E+ ++D TD+ +L A R + E Sbjct: 91 RLNIARYV--IEPTAREKTVANMAALHQKAQDFFDQFERCCISDDTDRRMLEADRRFFAE 148 Query: 146 FDKSLAEALKLANAGQWNEARALLANNQ-PVIDRLNKAMTELRAYNEKISQAGAHRAQET 204 ++K + L +AG+ + R + P RL + E + YNE++ + GA A E Sbjct: 149 YEKHRMAMVDLGSAGKEDALRVAMEEKMAPTGQRLMAVLNEHKNYNEQLGRQGAEAADEH 208 Query: 205 MAAARLHAILISAAVIAVVTLMGLLIARKIARSLAEAVELAETIAEGDLTREIDISSTDE 264 + + I + VI +V+ + LL+ R I SL A ELA+TI GDL+ +I + DE Sbjct: 209 LQLSIYLTIAAATLVIVMVSALSLLLVRNIRHSLHAATELADTIGRGDLSSQIVLRGRDE 268 Query: 265 IGHLMAAMAKMNRNLVTIVATVRTGVNTVAAASSQIATGNMDLSARTEQQASSLEQTAAA 324 + LM ++ +M +LV I+ V T+ A+ +IA+GN+DLS+RTE QA SLE+T +A Sbjct: 269 VTQLMLSLQRMTGSLVQILGKVNDSAATIETATREIASGNLDLSSRTEAQAGSLEETVSA 328 Query: 325 TEELTSTVKQNAENALQADLLASAASGIAAEGGEVVKQVVTTMEEIRSSSLNIADIISVI 384 +LT+TVKQNA+NA QAD LA +ASG+A + G+VV +V+ TME I +SSL I DIISVI Sbjct: 329 MGQLTTTVKQNADNARQADELAQSASGVAGQAGKVVDEVILTMEAINASSLKIVDIISVI 388 Query: 385 DGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSASAAKEIKQLIDDSVSKV 444 DGIAFQTNILALNAAVEAARAGEQGRGFAVVA+EVRSLAQRSASAA+EIK+LIDDSV+KV Sbjct: 389 DGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAREIKELIDDSVNKV 448 Query: 445 ENGSQLVMSAGNTMQQVVESVRRVTDVVTEISAASREQSLGIEQVNQAISHMDQSTQENA 504 ++GS+LV+ AG+TM++VV SVRRV+ +V +IS AS EQS GI QVNQAI MDQ+TQ+NA Sbjct: 449 QSGSRLVLQAGSTMEEVVTSVRRVSGIVRDISVASGEQSDGIAQVNQAIGMMDQTTQQNA 508 Query: 505 ALVEEATAAAQSLQEQARQLERAVNVFKLRQEAGA 539 ALVEEA AA+QSL+EQA+ L +AV+VF+L + A A Sbjct: 509 ALVEEAAAASQSLREQAKGLAQAVSVFRLERSAEA 543 >gi|300313897|ref|YP_003777989.1| methyl-accepting chemotaxis protein I [Herbaspirillum seropedicae SmR1] Length = 516 Score = 393 bits (1010), Expect = e-110, Method: Compositional matrix adjust. Identities = 266/521 (51%), Positives = 354/521 (67%), Gaps = 22/521 (4%) Query: 26 LSIVNRLYILIGAVIIGCVVLAGANMVKMNRTYADASFASENVVPSLLSLSTITQGINGM 85 L I +L + AVI+ V+L + ++ ++ + N +P++ + +I + M Sbjct: 7 LKIAKKLIVSFAAVIVLTVILGLFAIRELKAVNQASTDMATNWLPTIKAAQSIQASLPRM 66 Query: 86 RIATWRYLALTESVDRGAIESSFNSAKKRVEDALQK----YEQEQLADATDKELLRAVRV 141 RI+ + + +R E + + K R+ D L K YE Q+++A +K L Sbjct: 67 RISELQMVTAATPAER---EDALKAIKSRL-DILAKQRAAYEA-QISEAEEKVLYEKFSK 121 Query: 142 S---YQEFDKSLAEALKLANAGQWNEARALLANNQPVIDRLNKAMTELRAYNEKISQAGA 198 S Y E DK LA +++ G+ EARAL + ++ ++M E K++ AG+ Sbjct: 122 SFATYLEIDKELA---RISAEGKEEEARALFKGDS---NKTFRSMIENMDEIIKVNDAGS 175 Query: 199 HR----AQETMAAARLHAILISAAVIAVVTLMGLLIARKIARSLAEAVELAETIAEGDLT 254 R A++ +A L I + AA++ + + +++AR ++R L EAV +AE +A+GDLT Sbjct: 176 KRSDDMAEQVYRSATLWIIGLLAAIVVIALGLAVMVARAVSRPLNEAVAVAERVAQGDLT 235 Query: 255 REIDISSTDEIGHLMAAMAKMNRNLVTIVATVRTGVNTVAAASSQIATGNMDLSARTEQQ 314 +I +STDE G LM ++ MN +L IV VRTG +T+ AS +IATGN+DLS+RTE+Q Sbjct: 236 MDIRATSTDETGMLMHSLRAMNDSLQQIVGEVRTGTDTITTASQEIATGNLDLSSRTEEQ 295 Query: 315 ASSLEQTAAATEELTSTVKQNAENALQADLLASAASGIAAEGGEVVKQVVTTMEEIRSSS 374 A SLE+TA+A EELT+TVKQNA+NA QA+ LA++AS IA +GG VV QVV TME I SS Sbjct: 296 AGSLEETASAMEELTATVKQNADNARQANQLAASASDIATQGGAVVGQVVQTMEGITESS 355 Query: 375 LNIADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSASAAKEIK 434 IADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVA+EVRSLAQRSASAAKEIK Sbjct: 356 NRIADIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVASEVRSLAQRSASAAKEIK 415 Query: 435 QLIDDSVSKVENGSQLVMSAGNTMQQVVESVRRVTDVVTEISAASREQSLGIEQVNQAIS 494 QLIDDSV KV GSQLV AG TM +VV SVRRVTDVV EISAAS EQS GIE++N+AI+ Sbjct: 416 QLIDDSVEKVGAGSQLVERAGATMSEVVASVRRVTDVVAEISAASNEQSTGIEEINRAIT 475 Query: 495 HMDQSTQENAALVEEATAAAQSLQEQARQLERAVNVFKLRQ 535 MD+ TQ+NAALVEEA AAAQSLQ+QA +L + V+VFKL++ Sbjct: 476 QMDEVTQQNAALVEEAAAAAQSLQDQAARLSQVVSVFKLQR 516 >gi|300311614|ref|YP_003775706.1| methyl-accepting chemotaxis transducer transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 581 Score = 377 bits (967), Expect = e-105, Method: Compositional matrix adjust. Identities = 238/448 (53%), Positives = 309/448 (68%), Gaps = 11/448 (2%) Query: 100 DRGAIESSF---NSAKKRVEDALQKYEQEQLADATDKELLRAVRVSYQEFDKSLAEALKL 156 +R A E F A K+ ++AL K Q+ A ++ +A+ + FD S A+ L L Sbjct: 80 NRPANEKRFAQLEVAVKQTKEALDKITARQVT-AVGQKKAKALDEARIAFDASKADFLAL 138 Query: 157 ANAGQWNEARALLANNQPVIDRLNKAMTELRAY----NEKISQAGAHRAQETMAAARLHA 212 AG A + + + D N + RA+ + ++ GA + A + Sbjct: 139 VKAGNSTSADEYVL--RKITDTQNNYLALARAFADSQDAQLQAEGAKAVADGAMAIEVTL 196 Query: 213 ILISAAVIAVVTLMGLLIARKIARSLAEAVELAETIAEGDLTREIDISSTDEIGHLMAAM 272 AAV+A + ++G L+AR I + L EAV +AE +A GDLT I+ S DE G LM A+ Sbjct: 197 ACSMAAVLAAI-ILGYLMARSIVKPLTEAVRVAENVAAGDLTTRIEPHSRDETGQLMQAL 255 Query: 273 AKMNRNLVTIVATVRTGVNTVAAASSQIATGNMDLSARTEQQASSLEQTAAATEELTSTV 332 KMN NL+ IV+ VR G ++ AS +IA+GN+DLSARTEQQA SLE+TA+A EE+TSTV Sbjct: 256 RKMNDNLIEIVSGVRRGTESITTASGEIASGNLDLSARTEQQAGSLEETASAMEEMTSTV 315 Query: 333 KQNAENALQADLLASAASGIAAEGGEVVKQVVTTMEEIRSSSLNIADIISVIDGIAFQTN 392 +QNA+NA QA+ +A +AS IA +GG++V +VV TM+EI SS I DII VIDGIAFQTN Sbjct: 316 RQNADNARQANQMAVSASEIALQGGQIVGRVVHTMDEINQSSRKIVDIIGVIDGIAFQTN 375 Query: 393 ILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSASAAKEIKQLIDDSVSKVENGSQLVM 452 ILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSA+AAKEIK LI DSV KVE GS LV Sbjct: 376 ILALNAAVEAARAGEQGRGFAVVATEVRSLAQRSAAAAKEIKALIADSVGKVEGGSALVA 435 Query: 453 SAGNTMQQVVESVRRVTDVVTEISAASREQSLGIEQVNQAISHMDQSTQENAALVEEATA 512 AG TM+QVV SVR VTD+V EISAAS EQS GIE++N+A++ MD+ TQ+NAALVE+A A Sbjct: 436 QAGATMEQVVSSVRSVTDIVAEISAASAEQSSGIEEINRAVTQMDEGTQQNAALVEQAAA 495 Query: 513 AAQSLQEQARQLERAVNVFKLRQEAGAI 540 AA+S+QEQA +L +++++FKL Q A +I Sbjct: 496 AAKSMQEQAVRLTQSISIFKLEQGAASI 523 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.313 0.124 0.319 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,094,624 Number of extensions: 33036 Number of successful extensions: 500 Number of sequences better than 1.0e-08: 70 Number of HSP's gapped: 284 Number of HSP's successfully gapped: 71 Length of query: 564 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 474 Effective length of database: 1,193,720 Effective search space: 565823280 Effective search space used: 565823280 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 133 (55.8 bits)