BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0031.0059.p (591 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbasp... 1183 0.0 gi|300309596|ref|YP_003773688.1| acyl-CoA dehydrogenase [Herbasp... 523 e-149 gi|300310835|ref|YP_003774927.1| acyl-CoA dehydrogenase [Herbasp... 394 e-111 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 131 2e-31 gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbasp... 125 9e-30 >gi|300313303|ref|YP_003777395.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 591 Score = 1183 bits (3060), Expect = 0.0, Method: Compositional matrix adjust. Identities = 583/591 (98%), Positives = 584/591 (98%) Query: 1 MTMHYAPPLRDFAFVLDEVLQAPQVLRALPAHAEIDGDLMMQVLEEAGRFAADVVAPLNG 60 MTMHYAPPLRDFAFVLDEVLQAPQVLRALPAHAEIDGDLMMQVLEEAGRFAADVVAPLNG Sbjct: 1 MTMHYAPPLRDFAFVLDEVLQAPQVLRALPAHAEIDGDLMMQVLEEAGRFAADVVAPLNG 60 Query: 61 IGDREGCRFKNGTVTTPPGFERAYAQFCAGGWPALACAPEHGGQGLPHVLDCALYEMLSA 120 IGDREGCRFKNGTVTTPPGFERAYAQFCAGGWPALACAPEHGGQGLPHVLDCALYEMLSA Sbjct: 61 IGDREGCRFKNGTVTTPPGFERAYAQFCAGGWPALACAPEHGGQGLPHVLDCALYEMLSA 120 Query: 121 ANHGWTMYPGLLHGAYACLAAHGSAALQARYLDKIASGEWLATMCLTEAQAGSDLGQLRT 180 ANHGWTMYPGLLHGAYACLAAHGSAALQARYLDKIASGEWLATMCLTEAQAGSDLGQLRT Sbjct: 121 ANHGWTMYPGLLHGAYACLAAHGSAALQARYLDKIASGEWLATMCLTEAQAGSDLGQLRT 180 Query: 181 RAVPQDDGSYRLSGDKIFISGGEHDLTPNIVHLVLARLPDAPAGSKGLSLLLAPRVLDDG 240 RAVPQDDGSYRLSGDKIFISGGEHDLTPNIVHLVLARLPDAPAGSKGLSL LAPRVLDDG Sbjct: 181 RAVPQDDGSYRLSGDKIFISGGEHDLTPNIVHLVLARLPDAPAGSKGLSLFLAPRVLDDG 240 Query: 241 SRNGVHCTGIEHKMGIHGSATCSLHFEQAQAWLVGEAGRGLAAMFVMMNAARLHVGAQGL 300 SRNGVHCTGIEHKMGIHGSATCSLHFEQAQAWLVGEAGRGLAAMFVMMNAARLHVGAQGL Sbjct: 241 SRNGVHCTGIEHKMGIHGSATCSLHFEQAQAWLVGEAGRGLAAMFVMMNAARLHVGAQGL 300 Query: 301 GLAEAAYQRASAYIYRRRQSRAPGSSGNDLIVHHPAVQKLLMEQRCRIEGARLLTCWAGL 360 GLAEAAYQRASAY RRQSRAPGSSGNDLIVHHPAVQKLLMEQRCRIEGARLLTCWAGL Sbjct: 301 GLAEAAYQRASAYARERRQSRAPGSSGNDLIVHHPAVQKLLMEQRCRIEGARLLTCWAGL 360 Query: 361 LLDLAEHDPEAALRSRHHERLEFITPVIKAFLTDQGFQCASRALQVFGGHGYVVETGIEQ 420 LLDLAEHDPEAALRSRHHERLEFITPVIKAFLTDQGFQCASRALQVFGGHGYVVETGIEQ Sbjct: 361 LLDLAEHDPEAALRSRHHERLEFITPVIKAFLTDQGFQCASRALQVFGGHGYVVETGIEQ 420 Query: 421 FMRDARITMIYEGTNEIQAIDFLMRKVLGDEGRRLDDFLGLVRADIAEASGLGEQARLLG 480 FMRDARITMIYEGTNEIQAIDFLMRKVLGDEGRRLDDFLGLVRADIAEASGLGEQARLLG Sbjct: 421 FMRDARITMIYEGTNEIQAIDFLMRKVLGDEGRRLDDFLGLVRADIAEASGLGEQARLLG 480 Query: 481 EAVDKLRAFASRIAAAAVEAPALPYYVADEMLRLCGHVALGWMWLRAGRRALFAMAQDPL 540 EAVDKLR FASRIAAAA +APALPYYVADEMLRLCGHVALGWMWLRAGRRAL AMAQDPL Sbjct: 481 EAVDKLRIFASRIAAAAAQAPALPYYVADEMLRLCGHVALGWMWLRAGRRALSAMAQDPL 540 Query: 541 WYGGKRDAACYHFAFVFAEVQQLSSVIEACLATGLPPLPTDLDHHAAQEAS 591 WYGGKRDAACYHFAFVFAEVQQLSSVIEACLATGLPPLPTDLDHHAAQEAS Sbjct: 541 WYGGKRDAACYHFAFVFAEVQQLSSVIEACLATGLPPLPTDLDHHAAQEAS 591 >gi|300309596|ref|YP_003773688.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 596 Score = 523 bits (1346), Expect = e-149, Method: Compositional matrix adjust. Identities = 271/584 (46%), Positives = 364/584 (62%), Gaps = 33/584 (5%) Query: 4 HYAPPLRDFAFVLDEVLQAPQVLRALPAHAEIDGDLMMQVLEEAGRFAADVVAPLNGIGD 63 Y PLRD FVL E+L +V + LP HA+ + +L+ QVLEE G+FA+ V+ PLN GD Sbjct: 3 QYNAPLRDMQFVLHELLNVEEVFKELPPHADTNRELVDQVLEEGGKFASQVLFPLNHSGD 62 Query: 64 REGCRF--KNGTVTTPPGFERAYAQFCAGGWPALACAPEHGGQGLPHVLDCALYEMLSAA 121 REGC + ++ TVTTP GF++AY Q+ GW AL+C PE GGQGLP ++ + YEML+++ Sbjct: 63 REGCHYDRESKTVTTPKGFKQAYQQYVEAGWAALSCDPEFGGQGLPLTINNSFYEMLNSS 122 Query: 122 NHGWTMYPGLLHGAYACLAAHGSAALQARYLDKIASGEWLATMCLTEAQAGSDLGQLRTR 181 N WTMYPGL HGAY CL AHGS + YL K+ SGEW TMCLTEA G+DLG LR++ Sbjct: 123 NQAWTMYPGLSHGAYECLHAHGSEEQKQLYLPKLVSGEWTGTMCLTEAHCGTDLGLLRSK 182 Query: 182 AVPQDDGSYRLSGDKIFISGGEHDLTPNIVHLVLARLPDAPAGSKGLSLLLAPRVLDD-- 239 A PQ DGSY+++G+KIFIS GE+D++ NI+HLVLARLPDAP GSKG+SL L P+ L + Sbjct: 183 AEPQADGSYKITGNKIFISAGENDVSANIIHLVLARLPDAPEGSKGISLFLVPKFLPNAD 242 Query: 240 ---GSRNGVHCTGIEHKMGIHGSATCSLHFEQAQAWLVGEAGRGLAAMFVMMNAARLHVG 296 G+RN + C IE KMGIHG+ATC ++ + A WL+G+ +GL AMFV MNAARL VG Sbjct: 243 GSLGARNPIFCGAIEEKMGIHGNATCQMNLDGATGWLIGQPHKGLQAMFVFMNAARLGVG 302 Query: 297 AQGLGLAEAAYQRASAYIYRRRQSRA-----PGSSGNDLIVHHPAVQKLLMEQRCRIEGA 351 QGLGL E AYQ A Y R Q R+ D I+ HP V+++L+ + EG Sbjct: 303 MQGLGLTEVAYQNALVYAKDRLQMRSLSGIKAADKPADPIIVHPDVRRMLLTAKAFAEGG 362 Query: 352 RLLTCWAGLLLDLAEHDPEAALRSRHHERLEFITPVIKAFLTDQGFQCASRALQVFGGHG 411 R T + L LD P+ +R + + +TPV+KAFLTD + S LQV+GGHG Sbjct: 363 RAFTSYVALELDKELTHPDEQVRKDSADIVALLTPVVKAFLTDNAWMATSECLQVYGGHG 422 Query: 412 YVVETGIEQFMRDARITMIYEGTNEIQAIDFLMRKVLGDEGRRLDDFLGLVRADIAEASG 471 Y+ E G+EQ++RDARI MIYEGTN IQ++D L RK+L D G +L F ++A + E Sbjct: 423 YIAEWGMEQYVRDARINMIYEGTNTIQSLDLLGRKILMDNGAKLKKFGAQIQAFVEEHGT 482 Query: 472 LGEQARL------LGEAVDKL------RAFASRIAAAAVEAPALPYYVADEMLRLCGHVA 519 + +G+ V KL ++FA++ A A A+PY LR+ GH+ Sbjct: 483 DESMSEFITPLADIGDKVTKLTMEIGMKSFANQDEAGAA---AVPY------LRVVGHLV 533 Query: 520 LGWMWLRAGRRALFAMAQDPLWYGGKRDAACYHFAFVFAEVQQL 563 +++ R + AL +Y K A ++FA + E L Sbjct: 534 FSYLFARMAKIALEKQDSGDSFYAAKLATARFYFARLLPETAML 577 >gi|300310835|ref|YP_003774927.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 394 bits (1013), Expect = e-111, Method: Compositional matrix adjust. Identities = 229/538 (42%), Positives = 300/538 (55%), Gaps = 25/538 (4%) Query: 3 MHYAPPLRDFAFVLDEVLQAPQVLRALPAHAEIDGDLMMQVLEEAGRFAADVVAPLNGIG 62 M Y P++D FV++E L + ALP + + +LEE +F ++VVAPLN Sbjct: 1 MSYTAPVKDMLFVMNE-LAGLAAVNALPGCEDATPETAEAILEENAKFCSEVVAPLNHSA 59 Query: 63 DREGCRFKNGTVTTPPGFERAYAQFCAGGWPALACAPEHGGQGLPHVLDCALYEMLSAAN 122 D+E + +G VTT PGF+ A+ QF GW + E GGQGLP +L EML++AN Sbjct: 60 DKEPSSWADGKVTTSPGFKEAFRQFGEAGWQGVQHPVEFGGQGLPKLLATPCIEMLNSAN 119 Query: 123 HGWTMYPGLLHGAYACLAAHGSAALQARYLDKIASGEWLATMCLTEAQAGSDLGQLRTRA 182 + + P L G L G+ + ++ SG+W TM LTE QAGSDL +RTRA Sbjct: 120 LSFALCPLLTDGTIEALMTAGTQQQKETFIANFISGKWTGTMNLTEPQAGSDLALVRTRA 179 Query: 183 VPQDDGSYRLSGDKIFISGGEHDLTPNIVHLVLARLPDAPAGSKGLSLLLAPRVLDD--- 239 VP+ DG+Y+L G KIFI+ GEHD+ NIVHLVLAR P+AP G KG+SL + P+ L + Sbjct: 180 VPEGDGTYKLFGTKIFITYGEHDMAENIVHLVLARTPNAPEGVKGISLFIVPKFLVNADG 239 Query: 240 --GSRNGVHCTGIEHKMGIHGSATCSLHFEQ---AQAWLVGEAGRGLAAMFVMMNAARLH 294 G+RN VHC IEHK+GI S T L F A LVGE RGL MF+MMNAAR Sbjct: 240 SLGARNDVHCVSIEHKLGIKASPTAVLQFGDHGGAIGTLVGEENRGLEYMFIMMNAARFA 299 Query: 295 VGAQGLGLAEAAYQRASAYIYRRRQSR-APGSSGNDLIVHHPAVQKLLMEQRCRIEGARL 353 VG QGL ++E AYQ+A AY R QSR GS G I+H P V+++LM R +IE AR Sbjct: 300 VGIQGLSVSERAYQKAVAYARDRIQSRDLAGSPGPVAIIHQPDVRRMLMSMRAQIEAARA 359 Query: 354 LTCWAGLLLDLAEHDPEAALRSRHHERLEFITPVIKAFLTDQGFQCASRALQVFGGHGYV 413 L+ A D A + A R + E++ P++K ++T+ S +QV GG G++ Sbjct: 360 LSYVAAATSDAAHFSDDEATRKSNQAFYEYLVPIVKGWITEMSIDVTSTGVQVHGGMGFI 419 Query: 414 VETGIEQFMRDARITMIYEGTNEIQAIDFLMRKVLGDEGRRLDDFLGLVRADIAEASG-- 471 ETG Q+ RDARI IYEGT IQA D + RK D G L VR ++E G Sbjct: 420 EETGAAQYYRDARILPIYEGTTAIQANDLVGRKTARDGGAVAKGILAQVRKTVSELQGDA 479 Query: 472 -LGEQARLLGEAVDKLRAFASRIAA------AAVEAPALPYYVADEMLRLCGHVALGW 522 L A LGEA D L + A AV A ++PY L++ G V GW Sbjct: 480 QLQPIAARLGEAADALEQVVDYVVANVKSDIKAVFAGSVPY------LKMAGVVLGGW 531 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 131 bits (329), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 111/349 (31%), Positives = 161/349 (46%), Gaps = 40/349 (11%) Query: 97 CAPEH-GGQGLPHV-LDCALYEMLSAANHGWTMYPGLLHGAYAC--LAAHGSAALQARYL 152 C PE GG G+ ++ L AL E+ AA G T + + AC +GS + ++L Sbjct: 52 CVPEQWGGAGMDYMSLVLALEEI--AAGDGATSTIVSVQNSLACGITEKYGSPTQKEQWL 109 Query: 153 DKIASGEWLATMCLTEAQAGSDLGQLRTRAVPQDDGSYRLSGDKIFISGGEHDLTPNIVH 212 +A GE L CLTE GSD +RTRA +D + L+G K FI+ G+H V Sbjct: 110 KPLARGEMLGCFCLTEPHTGSDAAAIRTRA-ERDGDDFILNGTKQFITSGKH----AGVA 164 Query: 213 LVLARLPDAPAGSKGLSLLLAPRVLDDGSRNGVHCTGIEHKMGIHGSATCSLHFEQAQ-- 270 +V A + D AG KG+S L P G E KMG H S T + + + Sbjct: 165 IVFA-VTDRSAGKKGISCFLVP-----CDTPGFVVGRSEEKMGQHASDTVQIMLDNCRVP 218 Query: 271 -AWLVGEAGRGLAAMFVMMNAARLHVGAQGLGLAEAAYQRASAYIYRRRQSRAPGSSGND 329 L+G+ G G + A R+ + AQ +G+A AA++ A +Y +R P Sbjct: 219 ATALLGKEGEGYRIALSNLEAGRIGIAAQSVGMARAAFEAAVSYARQRESFGVP------ 272 Query: 330 LIVHHPAVQKLLMEQRCRIEGARLLTCWAGLLLDLAEHDPEAALRSRHHERLEFITPVIK 389 I+ H AV L + ++ ARL+ A L D + A + K Sbjct: 273 -IIEHQAVNFRLADMNTLLDAARLMVWRAAQLKDQGRPCLKEA-------------SMAK 318 Query: 390 AFLTDQGFQCASRALQVFGGHGYVVETGIEQFMRDARITMIYEGTNEIQ 438 F ++ + AS A+Q+ GG GY + +E+ RD RI IYEG N+IQ Sbjct: 319 MFASEAAEKIASDAIQIHGGVGYTSDFPVERIYRDVRICQIYEGANDIQ 367 >gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 125 bits (314), Expect = 9e-30, Method: Compositional matrix adjust. Identities = 113/372 (30%), Positives = 180/372 (48%), Gaps = 39/372 (10%) Query: 91 GWPALACAPEHGGQGLPHVLDCALYEMLSAANHGWTMYPGLLHGAYACLAAHGSAALQAR 150 G+ L GG GL + ++E L+ A Y + + +A+ G +L+A+ Sbjct: 49 GFCGLYTPEADGGLGLSRLDATVVFEELARACTSTAAYLTIHNMVSWMIASWGQPSLKAQ 108 Query: 151 YLDKIASGEWLATMCLTEAQAGSDLGQLRTRAVPQDDGSYRLSGDKIFISG-GEHDLTPN 209 + +++A+G + + CLTE +GSD L+T A +DG Y L+G K FISG G D Sbjct: 109 WCEQLAAGRKIGSYCLTEPGSGSDAASLKTTA-RLEDGHYVLNGSKAFISGAGSTD---- 163 Query: 210 IVHLVLARLPDAPAGSKGLSLLLAPRVLDDGSRNGVHCTGIEHKMGIHGSATCSLHFEQA 269 V +V+AR G++G+S ++ P G+ GV E KMG + T ++ F+ Sbjct: 164 -VLVVMART--GGEGARGVSAIVVP-----GNAPGVSYGKKESKMGWNSQPTRTISFDNV 215 Query: 270 QA---WLVGEAGRGLAAMFVMMNAARLHVGAQGLGLAEAAYQRASAYIYRRRQSRAPGSS 326 + L+G+ G+G ++ R+++ +G A+AA A AY+ R+Q P Sbjct: 216 KVPADHLLGQEGQGFIFAMKGLDGGRINIATCSVGAAQAALDAAHAYMKERKQFGRP--- 272 Query: 327 GNDLIVHHPAVQKLLMEQRCRIEGARLLTCWAGLLLDLAEHDPEAALRSRHHERLEFITP 386 I A+Q L + + + AR + A LD PEA +RL Sbjct: 273 ----IADFQALQFKLADMQTELVAARQMVRLAATKLDAGS--PEATTYCAMAKRLA---- 322 Query: 387 VIKAFLTDQGFQCASRALQVFGGHGYVVETGIEQFMRDARITMIYEGTNEIQAIDFLMRK 446 TD GF+ + ALQ+ GG+GY+ E +E++ RD R+ I EGTNEI + + RK Sbjct: 323 ------TDLGFRVCNEALQLHGGYGYIREYPLERYFRDVRVHQILEGTNEIMRV-IISRK 375 Query: 447 VLGDEGRRLDDF 458 +L + LDD Sbjct: 376 LLDRD--NLDDI 385 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.137 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,981,536 Number of extensions: 89049 Number of successful extensions: 290 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 268 Number of HSP's successfully gapped: 9 Length of query: 591 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 500 Effective length of database: 1,188,985 Effective search space: 594492500 Effective search space used: 594492500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 133 (55.8 bits)